BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0487.p (385 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312509|ref|YP_003776601.1| arabinose efflux permease [Herb... 724 0.0 gi|300312245|ref|YP_003776337.1| transmembrane efflux transmembr... 326 3e-90 gi|300313915|ref|YP_003778007.1| transmembrane arabinose efflux ... 121 1e-28 gi|300310405|ref|YP_003774497.1| arabinose efflux permease [Herb... 94 2e-20 gi|300312340|ref|YP_003776432.1| transporter transmembrane prote... 60 3e-10 >gi|300312509|ref|YP_003776601.1| arabinose efflux permease [Herbaspirillum seropedicae SmR1] Length = 385 Score = 724 bits (1868), Expect = 0.0, Method: Compositional matrix adjust. Identities = 369/385 (95%), Positives = 375/385 (97%) Query: 1 MNWPLFALAVAAFGIGTTEFVIMGLLPDVALDLGVTAPAAGMLVSGYALGVAAGAPILAI 60 MNWPLFALAVAAFGIGTTEFVIMGLLPDVA DLGVTAPAAGMLVSGYALGVAAGAPILAI Sbjct: 1 MNWPLFALAVAAFGIGTTEFVIMGLLPDVARDLGVTAPAAGMLVSGYALGVAAGAPILAI 60 Query: 61 LTAKWPRKQALVGLMCLFILGNVLCALAPNYPFLMVARVVTAFCHAAFFGIGSVVAAELV 120 LTAKWPRKQALVGLMCLFILGNVLCALAPNY FLMVARVVTAFCHAAFFGIGSVVAAELV Sbjct: 61 LTAKWPRKQALVGLMCLFILGNVLCALAPNYVFLMVARVVTAFCHAAFFGIGSVVAAELV 120 Query: 121 TPQKRAQAVALMFTGLTVANILGVPLGTAFGHAAGWRSTFGVVTGIGVLAAVMLWTMLPK 180 PQKRAQAVALMFTGLTVANILGVPLGTAFGHAAGWRSTFG VT IGVLAA+MLWTMLPK Sbjct: 121 APQKRAQAVALMFTGLTVANILGVPLGTAFGHAAGWRSTFGAVTAIGVLAAIMLWTMLPK 180 Query: 181 KIPMQAGNIFQEFRSIIDTRVQLSLLTSTLASISMFSVITYIAYILLDVTGFSAREESWV 240 KIPMQAGNIFQEFRSIIDTRVQLSLLTSTLASISMFSVITYIAYILLDVTGF+ ++ESWV Sbjct: 181 KIPMQAGNIFQEFRSIIDTRVQLSLLTSTLASISMFSVITYIAYILLDVTGFTTQQESWV 240 Query: 241 LLLFGAGITVGGLVGGKLADWKLMPVMCGVFLCIAIVLGLFSFTSHYKWPTLATMFAWGA 300 LLLFGAGITVGGLVGGKLADWKLMPVMCGVFLCIA+VLGLFS TSHYKWPTLATMFAWGA Sbjct: 241 LLLFGAGITVGGLVGGKLADWKLMPVMCGVFLCIAVVLGLFSITSHYKWPTLATMFAWGA 300 Query: 301 FAFAVVPGAQIRVLDTARGAPNLASTLNQGAFNLGNATGAWVGGLAISAGLPLTDLPLIG 360 FAFAVVPGAQIRVLDTARGAPNLASTLNQGAFNLGNATGAWVGGLAISAGLPLTDLPLIG Sbjct: 301 FAFAVVPGAQIRVLDTARGAPNLASTLNQGAFNLGNATGAWVGGLAISAGLPLTDLPLIG 360 Query: 361 SVFALLGLVTVAVSASLDKRVRAPA 385 + FALLGL TVA SASLDKRVRAPA Sbjct: 361 AFFALLGLATVAFSASLDKRVRAPA 385 >gi|300312245|ref|YP_003776337.1| transmembrane efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 395 Score = 326 bits (835), Expect = 3e-90, Method: Compositional matrix adjust. Identities = 189/374 (50%), Positives = 249/374 (66%) Query: 5 LFALAVAAFGIGTTEFVIMGLLPDVALDLGVTAPAAGMLVSGYALGVAAGAPILAILTAK 64 L+AL ++AF IGTTEFVIMGLLP+VA +LGV+ +AG+LV+GYALGV GAP+L T + Sbjct: 5 LWALTISAFAIGTTEFVIMGLLPEVAANLGVSLSSAGLLVTGYALGVFVGAPLLTATTGR 64 Query: 65 WPRKQALVGLMCLFILGNVLCALAPNYPFLMVARVVTAFCHAAFFGIGSVVAAELVTPQK 124 PRK LV LM +F++GN+ CA+AP+Y LM+ RVV +F H AFFG+GSVVA LV +K Sbjct: 65 LPRKTLLVALMVVFVVGNLACAIAPDYTTLMIGRVVASFAHGAFFGVGSVVATSLVPKEK 124 Query: 125 RAQAVALMFTGLTVANILGVPLGTAFGHAAGWRSTFGVVTGIGVLAAVMLWTMLPKKIPM 184 +A A+ALMFTGLTVAN+LGVP GT G + GWR+TF VT IGV+A ++P+ Sbjct: 125 QASAIALMFTGLTVANVLGVPFGTWLGQSYGWRATFWAVTVIGVIALAATAVLVPRSQGG 184 Query: 185 QAGNIFQEFRSIIDTRVQLSLLTSTLASISMFSVITYIAYILLDVTGFSAREESWVLLLF 244 + + +E R ++ +V L LL + L +F+ TYIA IL D++GF A S +LLLF Sbjct: 185 EETDFGKEVRVLMRPQVMLGLLMTVLGFGGVFAAFTYIAPILTDLSGFPASAVSPILLLF 244 Query: 245 GAGITVGGLVGGKLADWKLMPVMCGVFLCIAIVLGLFSFTSHYKWPTLATMFAWGAFAFA 304 G G+ +G L GGKLAD KLM + G + +A VL LF T K + + G AFA Sbjct: 245 GLGLVIGNLAGGKLADKKLMATLLGSLVLLAAVLVLFGLTVDNKVAAIIGVGLMGMAAFA 304 Query: 305 VVPGAQIRVLDTARGAPNLASTLNQGAFNLGNATGAWVGGLAISAGLPLTDLPLIGSVFA 364 VP Q+RVL+ A GAPNLAS+ N AFNLGNA GAW+GG+ I G L +P + ++ Sbjct: 305 TVPPLQMRVLEKATGAPNLASSANIAAFNLGNAAGAWLGGVTIDHGPGLHAVPYVAALLP 364 Query: 365 LLGLVTVAVSASLD 378 L G+V VS +D Sbjct: 365 LAGIVVALVSWKMD 378 >gi|300313915|ref|YP_003778007.1| transmembrane arabinose efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 121 bits (303), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 108/350 (30%), Positives = 170/350 (48%), Gaps = 21/350 (6%) Query: 8 LAVAAFGIGTTEFVIMGLLPDVALDLGVTAPAAGMLVSGYALGVAAGAPILAILTAKWPR 67 LA+ F IGT F+I LLP +A D G++ P LV + L +A +PI +L+A R Sbjct: 29 LALGTFAIGTEGFMIAPLLPRMAADFGMSVPMVASLVVIFTLVLALSSPISTVLSAAMNR 88 Query: 68 KQALVGLMCLFILGNVLCALAPNYPFLMVARVVTAFCHAAFFGIGSVVAAELVTPQKRAQ 127 ++ L+ M F N+L A + ++ L+VAR++ A + S +A +V P +R + Sbjct: 89 RRVLIAAMTAFTAANLLAACSSSFTGLLVARLLMAVAAGLYTPNASALAGAMVAPGQRGR 148 Query: 128 AVALMFTGLTVANILGVPLGTAFGHAAGWRSTFGVVTGIGVLAAVMLWTMLPKK--IPMQ 185 A+A++ G+T + LG+PLG GHA GWR+TF V+ +G +A + +P++ + Sbjct: 149 ALAIVSGGMTSPSALGLPLGAVVGHAFGWRATFLAVSAMGAVALAGILLGVPRQAGAHLH 208 Query: 186 AGNIFQEFRSIIDTRVQLSLLTSTLASISMFSVITYIAYILLDVTGFSAREESWVLLLFG 245 + + I +VQ L + L S+ ++ YIA L V F S + L+G Sbjct: 209 VAGLRERMAVIGQPQVQRLLAVTLLWSVGAYAAYPYIAPYLSHVLSFGESRISLTVSLWG 268 Query: 246 AGITVGGLVGGKLADWKLMPVMCGVFLCIAIVLGLFSFTSHYKW-------PTLATMFAW 298 A VG VGG L D + V L + ++L ++ + P L + W Sbjct: 269 AAAAVGVSVGGTLNDRYGSARVVRVSLLL-LMLSFWTLAAATALPPPLALGPVLLAVVVW 327 Query: 299 GAFAFAVVPGAQIRVLDTARGAPNLA--------STLNQGAFNLGNATGA 340 G +A P R++ GAP A STL G F+LG+A GA Sbjct: 328 GFSVWAFFPAQMARLIGA--GAPAQAPVALSLNTSTLYLG-FSLGSAAGA 374 >gi|300310405|ref|YP_003774497.1| arabinose efflux permease [Herbaspirillum seropedicae SmR1] Length = 422 Score = 93.6 bits (231), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 77/244 (31%), Positives = 124/244 (50%), Gaps = 5/244 (2%) Query: 2 NWP-LFALAVAAFGIGTTEFVIMGLLPDVALDLGVTAPAAGMLVSGYALGVAAGAPILAI 60 NW + +LA+ F + T EF+ LL +A DL V+ AAG V+ A+ A AP + + Sbjct: 35 NWSGIASLALGVFSLVTAEFLPASLLTAIASDLHVSDGAAGQAVTATAIVGAVAAPSIPL 94 Query: 61 LTAKWPRKQALVGLMCLFILGNVLCALAPNYPFLMVARVVTAFCHAAFFGIGSVVAAELV 120 LT RK+ ++ L L ++ N+L APN P L+VARV+ F+ + + +A LV Sbjct: 95 LTRNIDRKRVVLMLSLLLLVSNILAVTAPNLPVLLVARVLLGVALGGFWSMAAALAMRLV 154 Query: 121 TPQKRAQAVALMFTGLTVANILGVPLGTAFGHAAGWRSTFGVVTGIGVLAAVMLWTMLPK 180 A+A++L+ TG++VA + P+G G+ GWRS F + ++A + W +P+ Sbjct: 155 PDHLFARAMSLILTGVSVATVCAAPIGAYMGNLWGWRSAFVAAGVVSLVAIAIQWIAIPR 214 Query: 181 KIPMQAGN--IFQEFRSIIDTRVQLSLLTSTLASISMFSVITYIAYILLDVTGFSAREES 238 P + + E D RV L + ++ F+ TYI ++ +T S S Sbjct: 215 LPPKDHPSLKVLAELLGRKDVRVALLAVLLVVS--GHFAGFTYIRPLMEQITHMSVTTIS 272 Query: 239 WVLL 242 VLL Sbjct: 273 AVLL 276 >gi|300312340|ref|YP_003776432.1| transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 421 Score = 60.5 bits (145), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 59/254 (23%), Positives = 108/254 (42%), Gaps = 7/254 (2%) Query: 32 DLGVTAPAAGMLVSGYALGVAAGAPILAILTAKWPRKQALVGLMCLFILGNVLCALAPNY 91 + +T G++VS YA A + A ++ RK+ L+ F+LG V C LA ++ Sbjct: 51 SMQITPQQFGLVVSAYAFSAGASGLLTAGFADRFDRKRLLLFFYGGFVLGTVWCGLAQSF 110 Query: 92 PFLMVARVVTAFCHAAFFGIGSVVAAELVTPQKRAQAVALMFTGLTVANILGVPLGTAFG 151 L++AR+VT I +A +L P R + + ++ T + +LG+P+G Sbjct: 111 ESLLLARIVTGLFGGVIGSITLAIATDLFAPHLRGRVMGVIQTSFAASQVLGIPIGLYLS 170 Query: 152 HAAGWRSTFGVVTGIGVLAA-VMLWTMLPKK----IPMQAGNIFQEFRSIIDTRVQLSLL 206 + W F + +G+ V+L M P + + + ++ + R + Sbjct: 171 NRWNWHVPFLAMAALGLAGGLVVLVRMQPVDGHLTLKQEHSAWMHLYHTLAEPRYLTAFA 230 Query: 207 TSTLASISMFSVITYIAYILLDVTGFSAREESWVLLLFGAGITVGGLVGGKLADW--KLM 264 T+ L F ++ + + L+ G + L+ G G + G+ AD KL Sbjct: 231 TTALLMTGGFMLMPFSSAYLVGNLGIDMAHLPTIYLVTGLCTIAFGPLIGRAADKLGKLR 290 Query: 265 PVMCGVFLCIAIVL 278 + G L I +VL Sbjct: 291 VFLFGAVLSIIMVL 304 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.140 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,193,075 Number of extensions: 51764 Number of successful extensions: 433 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 430 Number of HSP's successfully gapped: 13 Length of query: 385 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 298 Effective length of database: 1,207,925 Effective search space: 359961650 Effective search space used: 359961650 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 131 (55.1 bits)