BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0465.p (204 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310565|ref|YP_003774657.1| response regulator containing a... 144 4e-36 gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protei... 113 1e-26 gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent p... 104 5e-24 gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protei... 102 2e-23 gi|300310279|ref|YP_003774371.1| HD-GYP domain-containing protei... 102 3e-23 >gi|300310565|ref|YP_003774657.1| response regulator containing a CheY-like receiver domain and an HD-GYP domain [Herbaspirillum seropedicae SmR1] Length = 348 Score = 144 bits (364), Expect = 4e-36, Method: Compositional matrix adjust. Identities = 82/192 (42%), Positives = 109/192 (56%), Gaps = 3/192 (1%) Query: 14 KAARQAAHDEQL-LMSLFLLAWAVEARDPYTGGHLWRVARLSRLLAHSAGLPAEQVARIT 72 + +Q H + L L S+ LLA A RDP TG HL R+A +R++A GL ++Q + Sbjct: 141 QVDQQVRHSDTLALDSVDLLARAAGYRDPETGEHLTRMANYARIIAARMGLDSQQQELLL 200 Query: 73 IGAFLHDLGKIGIPDAVLGKKSRLDEAEYAVIRTHPEIGHRMLAG--HPLAALAEEAVLL 130 A +HD+GK+GIPD +L K RLDE E V+R H IG +L PL A + L Sbjct: 201 NAAPMHDIGKMGIPDHILLKPGRLDEEELKVMRMHARIGADILVASPSPLLQTASQIALT 260 Query: 131 HHETPDGRGYPFGRRGEDIPLAARIVGICDAFDAMTSTRPYRSGMPIARAMEIIEGELGK 190 HHE DG GYP G +GE IPL RIV + D FDA+TS+RPY+ G + RA + + + G Sbjct: 261 HHEKWDGSGYPNGLKGEQIPLYGRIVAVADVFDALTSSRPYKPGWSLERASQYLREQSGA 320 Query: 191 QFDAELGRRFIG 202 FD F Sbjct: 321 HFDPRCVEAFFA 332 >gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 406 Score = 113 bits (283), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 60/163 (36%), Positives = 93/163 (57%), Gaps = 2/163 (1%) Query: 40 DPYTGGHLWRVARLSRLLAHSAGLPAEQVARITIGAFLHDLGKIGIPDAVLGKKSRLDEA 99 D YT H V + LA GL A++ + + LHD+GK+ +P ++L K S+L E Sbjct: 162 DNYTYMHSVAVCAMMISLADQLGLSAQERQQAGMAGLLHDIGKMTVPPSILNKPSKLTEE 221 Query: 100 EYAVIRTHPEIGHRMLAGHP-LAALAEEAVLLHHETPDGRGYPFGRRGEDIPLAARIVGI 158 E+ +++HP GHR+L P + + L HHE DG GYP+ G DI + AR+ I Sbjct: 222 EFVSVQSHPLAGHRLLQELPGIGDAPLDVSLHHHEKMDGTGYPYKLSGRDISMTARMGAI 281 Query: 159 CDAFDAMTSTRPYRSGMPIARAMEIIEGELGKQFDAELGRRFI 201 CD +DA+TS RPY+SG +R+++ + G FD ++ + F+ Sbjct: 282 CDVYDAITSDRPYKSGWDPSRSIQHMAQSKG-HFDFDVLQAFV 323 >gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent phosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 406 Score = 104 bits (260), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 60/163 (36%), Positives = 84/163 (51%), Gaps = 2/163 (1%) Query: 40 DPYTGGHLWRVARLSRLLAHSAGLPAEQVARITIGAFLHDLGKIGIPDAVLGKKSRLDEA 99 D YT H V L L + GL +G FLHD+GK IP +L K +L + Sbjct: 156 DEYTYLHSVAVCALMVALGRTLGLDETACREAGLGGFLHDVGKAFIPHELLNKPGKLSDE 215 Query: 100 EYAVIRTHPEIGHRMLAGHP-LAALAEEAVLLHHETPDGRGYPFGRRGEDIPLAARIVGI 158 E++ I+ HPE+G++ L+ P + A + L HHE DG GYP G R I L +R+ I Sbjct: 216 EFSQIKKHPELGYKYLSQDPNVPDYARDVCLHHHEKIDGNGYPEGLRAGAITLYSRMAAI 275 Query: 159 CDAFDAMTSTRPYRSGMPIARAMEIIEGELGKQFDAELGRRFI 201 CD +DA+TS RPY+ A A+ + G FD + F+ Sbjct: 276 CDVYDALTSDRPYKRAWDPAEAISAMASWTG-HFDRAMFLAFV 317 >gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 401 Score = 102 bits (254), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 55/167 (32%), Positives = 90/167 (53%), Gaps = 2/167 (1%) Query: 36 VEARDPYTGGHLWRVARLSRLLAHSAGLPAEQVARITIGAFLHDLGKIGIPDAVLGKKSR 95 ++ D YT H V + LA+ G+ +Q + + LHD+GK+ +P +L K ++ Sbjct: 155 LKTTDDYTYLHSVAVCAMMISLANQLGMTPQQTKQAGMAGLLHDVGKMAVPLDILNKPAK 214 Query: 96 LDEAEYAVIRTHPEIGHRMLAG-HPLAALAEEAVLLHHETPDGRGYPFGRRGEDIPLAAR 154 L E E+A +R H GH +L + +A + L HHE DG GYPF + + I + A+ Sbjct: 215 LTEEEFASVRMHTVQGHGILQQIEGIGEVALDVSLHHHEKIDGSGYPFNLKADSISIMAK 274 Query: 155 IVGICDAFDAMTSTRPYRSGMPIARAMEIIEGELGKQFDAELGRRFI 201 + +CD +DA+TS RPY++G AR++ + G FD + F+ Sbjct: 275 MGAVCDVYDAITSNRPYKAGWDPARSIRHMAASAG-HFDPITMQAFV 320 >gi|300310279|ref|YP_003774371.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 408 Score = 102 bits (253), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 59/167 (35%), Positives = 84/167 (50%), Gaps = 5/167 (2%) Query: 16 ARQAAHDEQLLMSLFLLAWAVEARDPYTGGHLWRVARLSRLLAHSAGLPAEQVARITIGA 75 A A D L+S+ L + D YT H V L LA + G+ + Sbjct: 130 ATSVARDSNALLSIIRL----KRVDEYTYLHSIAVCTLMVALARTLGMDDIACKEAGLAG 185 Query: 76 FLHDLGKIGIPDAVLGKKSRLDEAEYAVIRTHPEIGHRMLAGHP-LAALAEEAVLLHHET 134 +LHD+GK IP +L K RL E EY +I+ HP +G+ L G + + L HHE Sbjct: 186 YLHDVGKAFIPLEILNKPDRLSEQEYKIIQRHPALGYDYLKGFSGVPDYVLDVCLHHHEK 245 Query: 135 PDGRGYPFGRRGEDIPLAARIVGICDAFDAMTSTRPYRSGMPIARAM 181 DG GYP +G I + +R+ +CD +DA+TS RPY+SG A ++ Sbjct: 246 IDGSGYPSRHQGNQISVYSRMAAVCDVYDAVTSERPYKSGWDPAESL 292 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.139 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 665,622 Number of extensions: 27638 Number of successful extensions: 100 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 90 Number of HSP's successfully gapped: 10 Length of query: 204 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 123 Effective length of database: 1,236,335 Effective search space: 152069205 Effective search space used: 152069205 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 128 (53.9 bits)