BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0424.p (396 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312557|ref|YP_003776649.1| hydroxy-atrazine ethyl amino hy... 771 0.0 gi|300309804|ref|YP_003773896.1| hydroxydechloroatrazine ethylam... 236 2e-63 gi|300312562|ref|YP_003776654.1| guanine deaminase [Herbaspirill... 90 2e-19 gi|300311604|ref|YP_003775696.1| formiminoglutamate deiminase [H... 62 1e-10 >gi|300312557|ref|YP_003776649.1| hydroxy-atrazine ethyl amino hydrolase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 771 bits (1990), Expect = 0.0, Method: Compositional matrix adjust. Identities = 369/393 (93%), Positives = 383/393 (97%) Query: 1 VVIPGLINTHHHMYQSLTRAIPSAQNGELFNWLTNLYPIWAGLTPEMIRVSTLTAMAELI 60 +V+PGLINTHHHMYQSLTRAIPSAQNGELFNWLT LYPIWAGLTPEMI+VSTLTAMAELI Sbjct: 63 IVLPGLINTHHHMYQSLTRAIPSAQNGELFNWLTKLYPIWAGLTPEMIQVSTLTAMAELI 122 Query: 61 LSGCTTSSDHLYIYPNQTRLDDSLEAAAQIGMRFHGARGAMSVGLSKGGLPPDRVVEEEP 120 LSGCTTSSDHLYIYPNQTRLDDSLEAAA+IGMRFHGARGAMSVG SKGGLPPDRVV++E Sbjct: 123 LSGCTTSSDHLYIYPNQTRLDDSLEAAARIGMRFHGARGAMSVGQSKGGLPPDRVVQDEA 182 Query: 121 AILKDTQRLIETYHDSQRHAMQRIVVAPCSPFSVSRDLMREAADLARHYGVSLHTHLAEN 180 AILKDTQRLIETYHD QRHAMQRIVVAPCSPFSVSR+LMREAADLARHYGVSLHTHLAEN Sbjct: 183 AILKDTQRLIETYHDHQRHAMQRIVVAPCSPFSVSRELMREAADLARHYGVSLHTHLAEN 242 Query: 181 VNDIAYSREKFNMTPAQYAEGCGWVGHDVWHAHCVQLDEHGIDLFARTGTGVAHCPCSNM 240 VNDIAYSREKFNM+PA+YAE CGWVGHDVWHAHCVQLD HGIDLFARTGTGVAHCPCSNM Sbjct: 243 VNDIAYSREKFNMSPAEYAEDCGWVGHDVWHAHCVQLDAHGIDLFARTGTGVAHCPCSNM 302 Query: 241 RLASGIAPIRKMLDAGVPVGLGVDGSASNDSAHMLGEVRQAMLLQRVGFGPDAMTARQAL 300 RLASGIAPIRKMLDAGVPVGLGVDGSASNDSAHMLGEVRQAMLLQRVGFGPDAMTARQAL Sbjct: 303 RLASGIAPIRKMLDAGVPVGLGVDGSASNDSAHMLGEVRQAMLLQRVGFGPDAMTARQAL 362 Query: 301 EVATLGGAKVLNRDDIGALKPGMSADVVMFDTRQIAFAGALHDPVAALVFCTPANVNTSI 360 EVATLGGAKVLNRDDIGALKPGMSADVVMFDTRQIAFAGALHDPVAALVFCTPANV+ SI Sbjct: 363 EVATLGGAKVLNRDDIGALKPGMSADVVMFDTRQIAFAGALHDPVAALVFCTPANVDCSI 422 Query: 361 INGRVIVREGQLTTIDLGAVLERHNHLAYQLAE 393 INGRVIVREGQLT+I+LGAVLERHN L+ LA+ Sbjct: 423 INGRVIVREGQLTSIELGAVLERHNQLSLALAQ 455 >gi|300309804|ref|YP_003773896.1| hydroxydechloroatrazine ethylaminohydrolase [Herbaspirillum seropedicae SmR1] Length = 471 Score = 236 bits (602), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 150/404 (37%), Positives = 204/404 (50%), Gaps = 33/404 (8%) Query: 1 VVIPGLINTHHHMYQSLTRAIPSAQNGELFNWLT-NLYPIWAGLTPEMIRVSTLTAMAEL 59 V P +NTHHH++QSL + P N L WL Y A ++ R++ M EL Sbjct: 56 VAYPAWVNTHHHLFQSLLKGDPLGINATLTPWLAATPYRYRASFDADLFRLAARIGMVEL 115 Query: 60 ILSGCTTSSDHLYIYPNQTRLDDS---LEAAAQIGMRFHGARGA------MSVGLSKGGL 110 + SGC T +DH Y+Y D S E A Q+G+RF RG + LS Sbjct: 116 MRSGCGTIADHNYLYYPGMPFDTSEILFEEAQQLGLRFVLCRGTATRTRQLEAELSSALR 175 Query: 111 PPDRVVEEEPAILKDTQRLIETYHDSQRHAMQRIVVAPCSP-FSVSRDLMREAADLARHY 169 P E L D Q L +HD +M+R+V+AP +P +S + D +RE A AR Sbjct: 176 P-----ETLDGFLADMQGLAGRFHDPAPASMRRVVMAPTTPLYSAAPDELREMAVAAREM 230 Query: 170 GVSLHTHLAENVNDIAYSREKFNMTPAQYAEGCGWVGHDVWHAHCVQLDEHGIDLFARTG 229 G+ LH+HL+E V + P ++ E GW+G DVW AH V+LD I L TG Sbjct: 231 GLRLHSHLSETVGYQDSAHAMHGCRPVEFCERVGWLGRDVWFAHLVKLDAEEIQLLGGTG 290 Query: 230 TGVAHCPCSNMRLASGIAPIRKMLDAGVPVGLGVDGSASNDSAHMLGEVRQAMLLQRV-- 287 TG+AHCP SN RL SGIAPIR + AGVPV +GVDG+ASN++A M+ E A L+QR Sbjct: 291 TGIAHCPQSNGRLGSGIAPIRALEAAGVPVSIGVDGAASNEAADMISETHAAWLMQRARR 350 Query: 288 ------GF-------GPDAMTARQALEVATLGGAKVLNRDDIGALKPGMSADVVMFDTRQ 334 F G DA T + T GGA+VL D + L+ G AD+ ++ Sbjct: 351 GQEATPSFRGGHFEGGADAATVEDVVRWGTTGGARVLGLDALDGLQVGQQADIALYRLDD 410 Query: 335 IAFAGALHDP-VAALVFCTPANVNTSIINGRVIVREGQLTTIDL 377 + G LHDP + + + + GR +VRE + +DL Sbjct: 411 PRYFG-LHDPAIGPVASGGRPFLKAMWVGGRAVVREDAIPGVDL 453 >gi|300312562|ref|YP_003776654.1| guanine deaminase [Herbaspirillum seropedicae SmR1] Length = 432 Score = 90.1 bits (222), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 88/338 (26%), Positives = 154/338 (45%), Gaps = 25/338 (7%) Query: 1 VVIPGLINTHHHMYQSLTRAIPSAQNGELFNWLTNL-YPIWAGL-TPEMIRVSTLTAMAE 58 ++ PG I+TH H Q T I S G L WL N +P PE +AE Sbjct: 67 ILCPGFIDTHVHYPQ--TDIIGSPAPG-LLPWLDNYTFPAERRFQQPEHAAEVADFFLAE 123 Query: 59 LILSGCTTSSDHLYIYPNQTRLDDSLEAAAQIGMRFHGARGAMSVGLSKGGLPPDRVVEE 118 L+ +G T+++ + ++P +D A+ +R + M PD + + Sbjct: 124 LLRNGTTSAAVYCTVHPQS--VDAFFAASHARNLRMVAGKCLMDRH------APDFLRDT 175 Query: 119 EPAILKDTQRLIETYHDSQR-HAMQRIVVAPCSPFSVSRDLMREAADLARHY-GVSLHTH 176 + +++++ LI +H+ R H + AP S + + M+ A +LA Y + TH Sbjct: 176 AESGVRESEALIGRWHNKGRQHYAITVRFAPTS----THEQMKLAGELAAQYPDTYIQTH 231 Query: 177 LAENVNDIAYSREKF--NMTPAQYAEGCGWVGHDVWHAHCVQLDEHGIDLFARTGTGVAH 234 +AEN +++ + +E + + +G G + +AHC+ LD+ A T + Sbjct: 232 VAENTDEVKWVKELYPDARSYLDVYDGYGLMRQRALYAHCIWLDDQDRARMAATQSAATL 291 Query: 235 CPCSNMRLASGIAPIRKMLDAGVPVGLGVDGSASNDSAHMLGEVRQAMLLQRVGFGPDAM 294 CP SN+ L SG+ + A +P+ + D S ML + + + R+ G + Sbjct: 292 CPTSNLFLGSGLFDFARADAARLPLSVATDVGGGT-SFSMLQTMNELYKVARM--GGTYL 348 Query: 295 TARQALEVATLGGAKVLNRD-DIGALKPGMSADVVMFD 331 A++ +ATLGGA+ L + IG+ PG AD ++ D Sbjct: 349 PAQRMFYLATLGGARALQLEGTIGSFTPGCDADFIVLD 386 >gi|300311604|ref|YP_003775696.1| formiminoglutamate deiminase [Herbaspirillum seropedicae SmR1] Length = 482 Score = 61.6 bits (148), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 97/418 (23%), Positives = 159/418 (38%), Gaps = 47/418 (11%) Query: 2 VIPGLINTHHHMYQSLTRAIPSAQNG---ELFNWLTNLYPIWAGLTPEMIRVSTLTAMAE 58 V+PG+ N H H +Q + ++W +Y L P + E Sbjct: 69 VLPGMPNLHSHAFQRAMAGLAETMGSPADSFWSWRELMYRFAQRLQPRQLEAIARQTYIE 128 Query: 59 LILSGCTTSSDHLYIY--PNQTRLDDS-------LEAAAQ--IGMRFHGARGAMSVGLSK 107 ++ +G T+ + Y++ P+ D L+AA Q IGM + V + Sbjct: 129 MLKAGYTSVCEFHYLHHAPDGAPYADPAEMAWPLLQAAGQSGIGMTL------LPVLYQQ 182 Query: 108 GGL-------PPDRVVEEEPAILKDTQRLIETYHDSQRHAMQRIVVAPCSPFSVSRDLMR 160 GGL R V +L +RL++ ++ M+R VAP S +V+ + Sbjct: 183 GGLGGRPVSAEQRRFVAAPSWLLGLRERLLQHMPEND---MRRYGVAPHSLRAVAPTGLE 239 Query: 161 EA-ADLARHYGVS---LHTHLAENVNDIAYSREKFNMTPAQYAEGCGWVGHDVWHAHCVQ 216 E + LA G S +H H+AE + ++ P Q+ V H Sbjct: 240 ELLSGLAGQPGGSDAPIHIHVAEQLREVEDCLAWSGQRPVQWLLSSQPVDARWCLVHATH 299 Query: 217 LDEHGIDLFARTGTGVAHCPCSNMRLASGIAPIRKMLDAGVPVGLGVDGSASNDSAHMLG 276 +D+ A +G V CP + L G+ +++D+ G+G D SN + + Sbjct: 300 MDDEEYRAVAGSGAVVGLCPTTEANLGDGVIDAPRLIDSAARWGIGSD---SNIAISLRA 356 Query: 277 EVRQAMLLQRVGFGP-------DAMTARQALEVATLGGAKVLNRDDIGALKPGMSADVVM 329 E+R QR+ DA L L G + L PG AD+V+ Sbjct: 357 ELRLLEYAQRLHHRRRNVLAVIDAPAVADRLFCQALAGGAQAAGRAVAGLMPGQRADLVV 416 Query: 330 FDTRQIAFAG-ALHDPVAALVFCTPAN--VNTSIINGRVIVREGQLTTIDLGAVLERH 384 D + A + ++ALVFC + + GR +V+EG+ D RH Sbjct: 417 LDPDHVNLAQRSSTQLLSALVFCEHDGPMIGDVYVGGRQVVKEGRHALDDRAREDYRH 474 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,291,890 Number of extensions: 55590 Number of successful extensions: 164 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 154 Number of HSP's successfully gapped: 4 Length of query: 396 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 309 Effective length of database: 1,207,925 Effective search space: 373248825 Effective search space used: 373248825 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)