BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0422.p (400 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313822|ref|YP_003777914.1| N-carbamoyl-L-amino acid amidoh... 652 0.0 gi|300312555|ref|YP_003776647.1| bifunctional OHCU decarboxylase... 382 e-107 gi|300313936|ref|YP_003778028.1| amidohydrolase [Herbaspirillum ... 155 8e-39 >gi|300313822|ref|YP_003777914.1| N-carbamoyl-L-amino acid amidohydrolase [Herbaspirillum seropedicae SmR1] Length = 417 Score = 652 bits (1683), Expect = 0.0, Method: Compositional matrix adjust. Identities = 336/417 (80%), Positives = 362/417 (86%), Gaps = 17/417 (4%) Query: 1 MNAMDLSSQQAGQQAMQWAELLAQHSEAPGMLTRTYLTPTHQAAAAELTRWMEQSGLRVR 60 M A+ QQAG+QAMQWA+ LAQH+E+PGMLTRTYLTP HQAAAA L RWME++G+RVR Sbjct: 1 MKAVPEPFQQAGRQAMQWAQQLAQHTESPGMLTRTYLTPQHQAAAAALARWMEEAGMRVR 60 Query: 61 RDNAGNVIGRYEAAPGHEHRPALVTGSHFDTVRNAGKYDGNLGIVLPLACIAQWHRAGKR 120 RDNAGNVIGRYEAAPGHE P+ VTGSHFDTVRN G YDGNLGIVLPLACIA+WHR G+R Sbjct: 61 RDNAGNVIGRYEAAPGHESAPSFVTGSHFDTVRNGGWYDGNLGIVLPLACIARWHRQGQR 120 Query: 121 FAFPIEVIGFAEEEGVRFKATLLGSRTVAGTFDQAVLENVDGEGISMRQAIRAAGLDPAG 180 FAFPIEVIGFAEEEGVRFKATLLGSRTVAGTFDQAVLEN D +G++MR AIRAAGLDP G Sbjct: 121 FAFPIEVIGFAEEEGVRFKATLLGSRTVAGTFDQAVLENRDSDGVTMRAAIRAAGLDPDG 180 Query: 181 IAGDAWSPASVAAFVEVHIEQGPLLLNEGLPVGVVTAISGASRFMAEVRGLAGHAGTVPM 240 IA DAW P S+AAFVEVHIEQGPLLLNE LPVGVVTAISGASRFMAEV GLAGHAGTVPM Sbjct: 181 IAADAWQPGSMAAFVEVHIEQGPLLLNEDLPVGVVTAISGASRFMAEVHGLAGHAGTVPM 240 Query: 241 HLRRDAAMAAAEIGLYIERRCGIKPGLVGTMGILEVVQGAANVVPGLASFSIDIRAEEDA 300 HLRRDAAMAAAEIGLYIERRC IKP LVGTMG+LEVVQGAANVVPGLASFSIDIRAE+D+ Sbjct: 241 HLRRDAAMAAAEIGLYIERRCSIKPELVGTMGLLEVVQGAANVVPGLASFSIDIRAEDDS 300 Query: 301 DRLAAVADVRAEIERIAARRPL----------AAVXALAR-------AIETAGWPVRHLP 343 DRLAAVADV+AEI+RIAARR + A+V R +I AGWPVRHLP Sbjct: 301 DRLAAVADVKAEIDRIAARRQVQLALRQTHEAASVPCAPRLQAALAQSIAAAGWPVRHLP 360 Query: 344 SGAGHDAMAMAPLGEVAMLFVRCGNGGISHHPDETMSTEDACIAAQVFSHLVEQFQP 400 SGAGHDAMA+A L +VAMLFVRCGNGGISHHPDETM+ DA IAAQVFSHLVEQFQP Sbjct: 361 SGAGHDAMALAGLADVAMLFVRCGNGGISHHPDETMTEADAAIAAQVFSHLVEQFQP 417 >gi|300312555|ref|YP_003776647.1| bifunctional OHCU decarboxylase/N-carbamoyl-L-amino acid amidohydrolase [Herbaspirillum seropedicae SmR1] Length = 587 Score = 382 bits (982), Expect = e-107, Method: Compositional matrix adjust. Identities = 204/407 (50%), Positives = 267/407 (65%), Gaps = 21/407 (5%) Query: 12 GQQAMQWAELLAQHSEAPGMLTRTYLTPTHQAAAAELTRWMEQSGLRVRRDNAGNVIGRY 71 G MQWAE + SE G LT TYLT THQ AA++ +WM ++G++V D GNV+GRY Sbjct: 180 GATVMQWAEEIGALSEEEGALTCTYLTETHQRTAAQIAQWMREAGMQVHIDAVGNVVGRY 239 Query: 72 EAAPGHEHRPALVTGSHFDTVRNAGKYDGNLGIVLPLACIAQWHRAGKRFAFPIEVIGFA 131 ++ H L+TGSH+DTVRN GKYDG GI+LP+A + H G+ F +EVIGF+ Sbjct: 240 QSP--HPQAKTLLTGSHYDTVRNGGKYDGREGILLPIAVVKYLHERGETLPFHLEVIGFS 297 Query: 132 EEEGVRFKATLLGSRTVAGTFDQAVLENVDGEGISMRQAIRAAGLDPAGIAGDAWSPASV 191 EEEGVRFK+T LGS + G FD A+L+ D E ++MR+ I AG D A I A PA + Sbjct: 298 EEEGVRFKSTFLGSNAITGHFDMALLDLRDKEDVTMRELIIQAGHDVAAIPQIARDPAEI 357 Query: 192 AAFVEVHIEQGPLLLNEGLPVGVVTAISGASRFMAEVRGLAGHAGTVPMHLRRDAAMAAA 251 A+VEVHIEQGP+LL LPVG+VT+I+G+SR++ +++G+A HAGT PM +R+DAA AAA Sbjct: 358 LAYVEVHIEQGPVLLKRDLPVGIVTSIAGSSRYLVDLKGVASHAGTTPMDMRKDAAAAAA 417 Query: 252 EIGLYIERRCGIKP--GLVGTMGILEVVQGAANVVPGLASFSIDIRAEEDADRLAAVADV 309 EI LY+E+RC LVGT+G L+V +G+ NV+PG S+DIRA D RLAAV D+ Sbjct: 418 EIILYVEQRCSQDQHAALVGTVGQLQVPRGSTNVIPGACQLSLDIRAATDEVRLAAVKDI 477 Query: 310 RAEIERIAARR---------------PLAA--VXALARAIETAGWPVRHLPSGAGHDAMA 352 A+IE I RR P A + L+ A + AG P L SGAGHDAMA Sbjct: 478 LAKIEEICTRRSIDLKLEETLSAPAAPCAPWLMAQLSAATQRAGVPPFELASGAGHDAMA 537 Query: 353 MAPLGEVAMLFVRCGNGGISHHPDETMSTEDACIAAQVFSHLVEQFQ 399 M L +V MLF RCGNGGISH+P ETM+ +DA ++ Q+ + F+ Sbjct: 538 MVRLTDVCMLFTRCGNGGISHNPLETMTADDAEVSGQILLDFLRNFK 584 >gi|300313936|ref|YP_003778028.1| amidohydrolase [Herbaspirillum seropedicae SmR1] Length = 419 Score = 155 bits (391), Expect = 8e-39, Method: Compositional matrix adjust. Identities = 128/408 (31%), Positives = 189/408 (46%), Gaps = 40/408 (9%) Query: 4 MDLSSQQAGQQ---AMQWAELLAQH---SEAPGMLTRTYLTPTHQAAAAELTRWMEQSGL 57 MD+++Q + + A W L+ + A G + R LT + ++ W G+ Sbjct: 1 MDMNTQISTLRIDGARLWDSLMELARIGATAKGGVCRLALTDLDKQGRDLVSSWARAEGM 60 Query: 58 RVRRDNAGNVIGRYEAAPGHEH-RPALVTGSHFDTVRNAGKYDGNLGIVLPLACIAQWHR 116 V D GN+ R PG + P ++TGSH DT GK+DGN G++ + + ++ Sbjct: 61 SVVVDQIGNIFMR---RPGRNNALPPVMTGSHIDTQPTGGKFDGNYGVLAGIEVVRTLNQ 117 Query: 117 AGKRFAFPIEVIGFAEEEGVRFKATLLGSRTVAGTF--DQAVLENVDGEGISMRQAIRAA 174 + PIEV + EEG RF ++GS G F D A D EG +R + Sbjct: 118 HNIQTEAPIEVAVWTNEEGSRFVPVMMGSGVFCGAFTLDHA-YNATDVEGKKVRDELERI 176 Query: 175 GLDPAGIAGDAWSPASVAAFVEVHIEQGPLLLNEGLPVGVVTAISGASRFMAEVRGLAGH 234 G + GD + A+ E HIEQGP+L N +GVV A+ G S + V G H Sbjct: 177 GYIGEQVCGDH----PIGAYYEAHIEQGPVLENADTVIGVVPAVLGLSWYDCTVTGFESH 232 Query: 235 AGTVPMHLRRDAAMAAAEIG---LYIERRCGIKPGLVGTMGILEVVQGAANVVPGLASFS 291 AG PM +R+DA + I + I R P GT+G ++V+ + NV+PG FS Sbjct: 233 AGPTPMEIRKDALQVSTYIMQEVVAIAHR--YAPYGRGTVGQVQVMPNSRNVIPGQVKFS 290 Query: 292 IDIRAEEDA-------DRLAAVADVRA------EIERIAARRPLA----AVXALARAIET 334 ID+R +A D LA + +R E+ER++ P V + A Sbjct: 291 IDLRNVSEASLTRMHEDLLAYLEAMRKKTGLSIELERVSYFPPCPFHPDCVDKVRAASAA 350 Query: 335 AGWPVRHLPSGAGHDAMAMAPLGEVAMLFVRCGNGGISHHPDETMSTE 382 G+ + SGAGHDA+ A L M+FV C + G+SH+ E E Sbjct: 351 LGYKTMDVVSGAGHDAIYAARLAPAGMIFVPCKD-GVSHNEIEDARPE 397 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,276,895 Number of extensions: 56417 Number of successful extensions: 183 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 175 Number of HSP's successfully gapped: 3 Length of query: 400 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 313 Effective length of database: 1,207,925 Effective search space: 378080525 Effective search space used: 378080525 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)