BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0421.p (443 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313823|ref|YP_003777915.1| C4-dicarboxylate transport tran... 768 0.0 gi|300311930|ref|YP_003776022.1| C4-dicarboxylate transport tran... 393 e-110 gi|300313118|ref|YP_003777210.1| proton glutamate symport protei... 214 1e-56 >gi|300313823|ref|YP_003777915.1| C4-dicarboxylate transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 445 Score = 768 bits (1983), Expect = 0.0, Method: Compositional matrix adjust. Identities = 417/442 (94%), Positives = 428/442 (96%) Query: 1 VSKVFKSLFGQVVLALVAGIVIGLLWPDFGQSLKPLGDGFIKLIKMIIPVIVFCVVVQGI 60 VSK+FKSLFGQVVLAL+ GI+IGL WPDFGQ+LKPLGDGFIKLIKMIIPVIVFCVVVQGI Sbjct: 2 VSKLFKSLFGQVVLALIGGIIIGLFWPDFGQNLKPLGDGFIKLIKMIIPVIVFCVVVQGI 61 Query: 61 CGASDLKKVGSVGVKAIIYFEVVTTIALLLGLVLALVVHPGAGMNIDPSNLDASSLSGYM 120 CGASDLKKVGSVGVKAIIYFEVVTTIALLLGLVLALVV PGAGMNIDPSNLDASSLSGYM Sbjct: 62 CGASDLKKVGSVGVKAIIYFEVVTTIALLLGLVLALVVQPGAGMNIDPSNLDASSLSGYM 121 Query: 121 ANAGKVKETGFADFIMKLIPATAVSAFTSGDVLQVLLISVTFGCALLLIGDKGAPVVALV 180 ANAGKVKETGFA+FIMKLIPATAVSAFTSGDVLQVLLISVTFGCALLLIG+KGAPVVALV Sbjct: 122 ANAGKVKETGFAEFIMKLIPATAVSAFTSGDVLQVLLISVTFGCALLLIGEKGAPVVALV 181 Query: 181 SSLSDAFFKCMSFFIRLAPLGVLGAIAFTVGKYGIGSLKQLALLVLLFYGAAIFFVLVVL 240 +SLSDAFFKCMSFFIRLAPLGVLGAIAFTVGKYGIGSLKQLALLVLLFYGA IFFVLVVL Sbjct: 182 ASLSDAFFKCMSFFIRLAPLGVLGAIAFTVGKYGIGSLKQLALLVLLFYGAVIFFVLVVL 241 Query: 241 GGILRASGLSIFKLIRYLREELVVVLATTSSDSVLPQIMRKLEHMGIKKSTVGLVIPTGY 300 GGILRASGLSIFKLIRYLREELVVVLATTSSDSVLPQIM+KLEHMGIKKSTVGLVIPTGY Sbjct: 242 GGILRASGLSIFKLIRYLREELVVVLATTSSDSVLPQIMKKLEHMGIKKSTVGLVIPTGY 301 Query: 301 SFNLDAFSIYLTMAALFIAQATNTHLSWGDLAAILAIALVTSKGAHGVPGSAIVILAATL 360 SFNLDAFSIYLTMAALFIAQATNTHLS GDLAAILAIALVTSKGAHGVPGSAIVILAATL Sbjct: 302 SFNLDAFSIYLTMAALFIAQATNTHLSMGDLAAILAIALVTSKGAHGVPGSAIVILAATL 361 Query: 361 TTIPAIPVVGLVLVLSIDWFIGIARALGNLLGNCVATVVIASWEKDIDKQRARAVLNGEY 420 TTIPAIPVVGLVLVLSIDWFIGIARALGNLLGNCVATVVIASWE+DIDK RARAVLNGE Sbjct: 362 TTIPAIPVVGLVLVLSIDWFIGIARALGNLLGNCVATVVIASWERDIDKVRARAVLNGES 421 Query: 421 VTPLAEELPVIDLPGVAGNPAR 442 V PL EE PVI+L GV PA+ Sbjct: 422 VAPLVEENPVIELMGVTVKPAQ 443 >gi|300311930|ref|YP_003776022.1| C4-dicarboxylate transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 441 Score = 393 bits (1010), Expect = e-110, Method: Compositional matrix adjust. Identities = 212/429 (49%), Positives = 302/429 (70%), Gaps = 3/429 (0%) Query: 4 VFKSLFGQVVLALVAGIVIGLLWPDFGQSLKPLGDGFIKLIKMIIPVIVFCVVVQGICGA 63 ++KSL+ QV++A+V GI++G +P G+++KPLGDGF+KLIKMII ++FC VV GI G Sbjct: 8 LYKSLYFQVLVAIVIGILLGHFYPSTGEAMKPLGDGFVKLIKMIIAPVIFCTVVIGIAGM 67 Query: 64 SDLKKVGSVGVKAIIYFEVVTTIALLLGLVLALVVHPGAGMNIDPSNLDASSLSGYMANA 123 D+KKVG G A++YFEVV+T+AL++GL+L + PG GMN+DP++LD S++ Y A Sbjct: 68 EDMKKVGKTGGLALLYFEVVSTVALIIGLLLVNFLQPGVGMNVDPASLDTKSIAAYTA-P 126 Query: 124 GKVKETGFADFIMKLIPATAVSAFTSGDVLQVLLISVTFGCALLLIGDKGAPVVALVSSL 183 GK+ DF++ +IP + V AF GDVLQVLL++V FG AL G +G V + + Sbjct: 127 GKMGSV--TDFVLGIIPTSMVDAFAKGDVLQVLLVAVLFGFALHKFGGRGTLVFDFIEKI 184 Query: 184 SDAFFKCMSFFIRLAPLGVLGAIAFTVGKYGIGSLKQLALLVLLFYGAAIFFVLVVLGGI 243 S F + +++AP+G GA++FT+GKYG+GSL LA L+ FY + F+ VVLG I Sbjct: 185 SHVLFSVVGAIMKVAPIGAFGAMSFTIGKYGVGSLFSLAKLMGTFYLTCLLFIFVVLGII 244 Query: 244 LRASGLSIFKLIRYLREELVVVLATTSSDSVLPQIMRKLEHMGIKKSTVGLVIPTGYSFN 303 R G SI+K ++Y++EEL++VL T+SS+SVLP+++ K+E+ G KK+ VGLVIPTGYSFN Sbjct: 245 TRLHGFSIWKFVKYIKEELLIVLGTSSSESVLPRMLAKMENAGAKKTVVGLVIPTGYSFN 304 Query: 304 LDAFSIYLTMAALFIAQATNTHLSWGDLAAILAIALVTSKGAHGVPGSAIVILAATLTTI 363 LD +IYLTMAA+FIAQATNT +++ +L + L+TSKGA G+ GS ++LAATL+ + Sbjct: 305 LDGTAIYLTMAAVFIAQATNTPMTFVQELTLLGVLLLTSKGAAGITGSGFIVLAATLSAV 364 Query: 364 PAIPVVGLVLVLSIDWFIGIARALGNLLGNCVATVVIASWEKDIDKQRARAVLNGEYVTP 423 +PV GL L+L ID F+ ARAL N +GN VAT+V+A W ++D +R VLN E V Sbjct: 365 GHVPVAGLALILGIDRFMSEARALTNTIGNGVATLVVAKWSGELDSERLTKVLNNETVED 424 Query: 424 LAEELPVID 432 ++D Sbjct: 425 AQAPESILD 433 >gi|300313118|ref|YP_003777210.1| proton glutamate symport protein [Herbaspirillum seropedicae SmR1] Length = 431 Score = 214 bits (545), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 143/404 (35%), Positives = 229/404 (56%), Gaps = 28/404 (6%) Query: 15 ALVAGIVIGLL---WPDFGQSLKPLG------DGFIKLIKMIIPVIVFCVVVQGICGASD 65 A++ GI++G L D Q+ + G D F++LIKM+I +VF +V GI D Sbjct: 14 AMLLGILVGYLIFSHNDKAQAKELAGYISIASDLFLRLIKMVIAPLVFSTLVVGIAHMGD 73 Query: 66 LKKVGSVGVKAIIYFEVVTTIALLLGLVLALVVHPGAGMNIDPSNLDASSLSGYMANAGK 125 K VG + K++ +F + + ++L LG+++A ++ PGAG+ + PS DA+ +G + Sbjct: 74 AKSVGRIFGKSLAWFFIASLVSLALGMIMANLLQPGAGVAL-PSP-DAAG-AGLATSKFT 130 Query: 126 VKETGFADFIMKLIPATAVSAFTSGDVLQVLLISVTFGCALLLIGDKGAPVVALVSSLSD 185 VKE F L+P + V A +VLQV++ S+ FG AL +G++G ++A++ LS Sbjct: 131 VKE-----FFNHLVPKSIVEAMAQNEVLQVVVFSMFFGIALASLGERGKHLLAVIDDLSH 185 Query: 186 AFFKCMSFFIRLAPLGVLGAIAFTVGKYGIGSLKQLALLVLLFYGAAIFFVLVVLGGILR 245 K + ++ AP+ V A+A TV G+ L A+ + FY F LV+L IL Sbjct: 186 TMLKITVYVMKFAPVAVFAAMAATVAVNGLEILVSFAVFMRDFY-----FSLVLLWVILI 240 Query: 246 ASGL-----SIFKLIRYLREELVVVLATTSSDSVLPQIMRKLEHMGIKKSTVGLVIPTGY 300 A G IF L+ ++E ++ AT SS++ P+++ L+ G+K+ V+P GY Sbjct: 241 AVGFIFLKKRIFHLLALIKEAFLLAFATASSEAAYPKLLDALDRFGVKRKISSFVMPMGY 300 Query: 301 SFNLDAFSIYLTMAALFIAQATNTHLSWGDLAAILAIALVTSKGAHGVPGSAIVILAATL 360 SFNLD IY T A LFIAQA H+ ++ + ++TSKG GVP +++V++AATL Sbjct: 301 SFNLDGSMIYCTFATLFIAQAYGIHMPISTQITMMLVLMLTSKGIAGVPRASLVVIAATL 360 Query: 361 TTIPAIPVVGLVLVLSIDWFIGIARALGNLLGNCVATVVIASWE 404 IP GL+++L ID F+ + R+ N +GN +A+ V+A WE Sbjct: 361 HQFN-IPEAGLLVILGIDTFLDMGRSATNAVGNSIASAVVAKWE 403 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.142 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,299,843 Number of extensions: 53342 Number of successful extensions: 279 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 274 Number of HSP's successfully gapped: 3 Length of query: 443 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 355 Effective length of database: 1,203,190 Effective search space: 427132450 Effective search space used: 427132450 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)