BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0376.p (255 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313865|ref|YP_003777957.1| 3-alpha-hydroxysteroid dehydrog... 435 e-123 gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogena... 64 1e-11 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 57 1e-09 >gi|300313865|ref|YP_003777957.1| 3-alpha-hydroxysteroid dehydrogenase/carbonyl reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 255 Score = 435 bits (1119), Expect = e-123, Method: Compositional matrix adjust. Identities = 211/255 (82%), Positives = 222/255 (87%) Query: 1 MTLQDKTIVVTGVSSGIGAETARLLRVQGARVIGVDRNAPIISVEGFVQADLSTADAIHD 60 M LQDKT+VVTGVSSGIGAETAR LR QGARVIGVDRN P ISVE FVQADLS+A+AI Sbjct: 1 MNLQDKTVVVTGVSSGIGAETARQLRAQGARVIGVDRNEPAISVETFVQADLSSAEAIDA 60 Query: 61 AAAQLPEKIDGLCNIAGVPGTAPADLVARVNYLGLRHFTELLIRRMPPGSAIVNVASILG 120 AQLPE+ID LCNIAGVPGTA A LVARVNYLGLRH + L R+ PG AIVNVASILG Sbjct: 61 MVAQLPERIDALCNIAGVPGTAEAQLVARVNYLGLRHLVQRLTARLSPGGAIVNVASILG 120 Query: 121 AEWPQRLDQHKALAQTAGFAAGAEWLAQHPVPQERCYQYFKEALIVWSMAQAQPWFLKHS 180 AEWPQRL+QHKAL+Q A FA GA WLAQHPV QE CYQYFKEALIVWSM QAQP FL+HS Sbjct: 121 AEWPQRLEQHKALSQAASFAEGAAWLAQHPVAQEHCYQYFKEALIVWSMTQAQPMFLQHS 180 Query: 181 IRMNSVAPGPVLTPILGDFVTMLGQDRVQKDAHRMKRPAYPDEIAAAIVWLCDDGSRWIN 240 +RMN VAPGPV TPILGDFVTMLGQ+RVQKDAHRMKRPAY DEIAAAIVWLC D SRWIN Sbjct: 181 VRMNCVAPGPVFTPILGDFVTMLGQERVQKDAHRMKRPAYADEIAAAIVWLCSDDSRWIN 240 Query: 241 GVNLPVDGGLASTYL 255 GVNLPVDGGLASTYL Sbjct: 241 GVNLPVDGGLASTYL 255 >gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 63.9 bits (154), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 80/272 (29%), Positives = 112/272 (41%), Gaps = 56/272 (20%) Query: 3 LQDKTIVVTGVSSGIGAETARLLRVQGARVIGVDRNAPIISVEGFVQADLSTADAIHDAA 62 +DK +VVTG S GIG TA+L +GARV R A +E V+ + A + A Sbjct: 7 FEDKVVVVTGGSEGIGLATAKLFAAEGARVYVSGRRAD--KLEQAVREIGNGAVGVPGDA 64 Query: 63 AQLPE-------------KIDGLCNIAGVPGTAPA----------DLVARVNYLGLRHFT 99 A+L + ++D L AG T D + +N GL Sbjct: 65 AKLDDLDRLYARVQADHGRLDVLFANAGTASTEAKPLGMIGEEDFDGLFNLNVRGLLFSV 124 Query: 100 ELLIRRMPPGSAIVNVASILGAEWPQRLDQHKALAQTAGFAAGAEWLAQHPVPQERCYQY 159 + + M G AIV S+ G + GF P + Y Sbjct: 125 QKALPLMSQGGAIVLNGSVAGVK---------------GF------------PGQSLYNA 157 Query: 160 FKEALIVWSMAQAQPWFLKH-SIRMNSVAPGPVLTPILGDFVTML-GQDRVQKDAHRMKR 217 K A V S A++ LK IR+N VAPG T ++ D++ G + V K + R Sbjct: 158 SKAA--VRSFARSWTADLKERGIRVNVVAPGGTETRLMRDYLEARPGMEDVLKQVVPLGR 215 Query: 218 PAYPDEIAAAIVWLCDDGSRWINGVNLPVDGG 249 PDEI A+++L S +I GV L VDGG Sbjct: 216 LGEPDEIGRAVLFLASAESSYIAGVELFVDGG 247 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 57.0 bits (136), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 76/274 (27%), Positives = 120/274 (43%), Gaps = 63/274 (22%) Query: 6 KTIVVTGVSSGIGAETARLLRVQGARVIGVDRN--APIISVEGFV---------QADLST 54 + +VTG + GIGA ++ L +G V+ D N A + + V Q DL + Sbjct: 9 RVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHRAAPLQMDLGS 68 Query: 55 ADAIHDAAAQLPE---KIDGLCNIAGVPGTA-----PAD---LVARVNYLGL----RHFT 99 ++I A A + + + D L N AGV T P + VN G+ +H Sbjct: 69 PESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGVLLAGQHAA 128 Query: 100 ELLIRRMPPGSAIVNVASILGAEWPQRLDQHKALAQTAGFAAGAEWLAQHPVPQERCYQY 159 L++++ IVN++SI +G AGA A Y Sbjct: 129 RLMVKQQW--GRIVNISSI------------------SGIRAGAGRTA---------YGT 159 Query: 160 FKEALIVWSMAQAQPWFLKHSIRMNSVAPGPVLTPILGDFVTMLGQDRVQKDAHR---MK 216 K A+I + Q ++ I +NSVAPGPV TP+ + ++ ++ +R M+ Sbjct: 160 SKAAVIGLTR-QMAIELAQYGITVNSVAPGPVDTPM----TQAMHSEQTRESYYRLVPMR 214 Query: 217 RPAYPDEIAAAIVWLCDDGSRWINGVNLPVDGGL 250 R DEIA+A+ +L + S +I G +PVDGG Sbjct: 215 RYGTTDEIASAVCYLAAEESAYITGHVIPVDGGF 248 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 853,024 Number of extensions: 35926 Number of successful extensions: 229 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 229 Number of HSP's successfully gapped: 6 Length of query: 255 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 172 Effective length of database: 1,226,865 Effective search space: 211020780 Effective search space used: 211020780 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)