BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0375.p (489 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Her... 813 0.0 gi|300310482|ref|YP_003774574.1| vanillin: NAD dehydrogenase oxi... 255 6e-69 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 253 2e-68 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 244 1e-65 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 244 1e-65 >gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 487 Score = 813 bits (2100), Expect = 0.0, Method: Compositional matrix adjust. Identities = 409/485 (84%), Positives = 446/485 (91%) Query: 5 STPLLDPARWQRHLFRGRWEETAHGTTVIEPATGAPLTEVGLAGADDVAISVQAAVAAQA 64 ++P LD A+WQ+HLFRG+W A G TV+EPATG PLT+VGLA DDV+ SV+ A+AAQA Sbjct: 3 ASPFLDSAQWQQHLFRGQWVAAAQGATVLEPATGRPLTQVGLASPDDVSASVELAIAAQA 62 Query: 65 EWLAVPPRERAEIFRRAGNLFQQHFDELARMVARETGGVLFKGEHEVREAITLCHLAAGM 124 W+A+PPRERA+IFRRA LF QHFDELARMVARETGGVLFKGEHEVREAITLCHLAAGM Sbjct: 63 AWVAMPPRERADIFRRAAGLFHQHFDELARMVARETGGVLFKGEHEVREAITLCHLAAGM 122 Query: 125 PLQAQGQLLPSAPGRLSIAKRAPLGVIGVISPFNFPLILTLRTVAPALAAGNAVVVKPDV 184 PLQAQGQLLPS PGRLSIA+RAPLGVIGVISPFNFPLILTLRTVAPALAAGNAVVVKPD+ Sbjct: 123 PLQAQGQLLPSTPGRLSIARRAPLGVIGVISPFNFPLILTLRTVAPALAAGNAVVVKPDL 182 Query: 185 RTPVSGGFMLARIFEEAGLPAGLLHVLPGGAEAGEALVTAPQVPMIAFTGSPAVGRRIGE 244 RTPVSGGFMLARIFE+AGLPAGLLHVLPGGAE GEALVTAP VPMIAFTGSPAVGRRIGE Sbjct: 183 RTPVSGGFMLARIFEQAGLPAGLLHVLPGGAETGEALVTAPGVPMIAFTGSPAVGRRIGE 242 Query: 245 LAGRHLKKVSLELGGANALVILDDADLDVAASNAAWGAWLHQGQICMAANRILVHASQAA 304 LAGRHLKK+SLELGGANALVIL+DADLDVAAS+AAWGAWLHQGQICMAANRILVHAS Sbjct: 243 LAGRHLKKLSLELGGANALVILEDADLDVAASHAAWGAWLHQGQICMAANRILVHASLEE 302 Query: 305 GLLERLVDKARHLPVGDGASGQVALGPLIDARQLQRVHGLVQDSVAAGARLLAGGSYEGL 364 GLL+RL DKARHLPVGDGASGQVALGP+ID RQLQRVH LVQ+SVAAGA+L+AGG YE L Sbjct: 303 GLLQRLADKARHLPVGDGASGQVALGPMIDERQLQRVHALVQESVAAGAQLVAGGRYEQL 362 Query: 365 FYQPTVLSGVRPGMRVFDEEIFGPVASVITVDSEEEAITLANQNQGGLAAGVISGSLARA 424 FYQPTVL GVRPGMRVF+EEIFGPVASVI D++E+AI LAN++ GGLAAGV+S S+ RA Sbjct: 363 FYQPTVLGGVRPGMRVFEEEIFGPVASVIRFDNDEDAIALANRHAGGLAAGVLSPSVGRA 422 Query: 425 LRVAGRLRQGMVHVNDQTVNDECVNPFGGPGIAGNGTSVGGPADWEEYTQWKWITVKEQA 484 + VA RLRQGMVHVNDQTVNDECVNPFGGPGIAGNG SVGGPADWEEYTQW+W T+++QA Sbjct: 423 MAVAARLRQGMVHVNDQTVNDECVNPFGGPGIAGNGASVGGPADWEEYTQWRWTTIRQQA 482 Query: 485 TPYPF 489 PYPF Sbjct: 483 MPYPF 487 >gi|300310482|ref|YP_003774574.1| vanillin: NAD dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 483 Score = 255 bits (651), Expect = 6e-69, Method: Compositional matrix adjust. Identities = 164/424 (38%), Positives = 235/424 (55%), Gaps = 7/424 (1%) Query: 71 PRERAEIFRRAGNLFQQHFDELARMVARETGGVLFKGEHEVREAITLCHLAAGMPLQAQG 130 P +R + A + Q D+ A+ +A ETG V+ A AA M Q G Sbjct: 62 PGQRRALLTHAADALQARADDFAQAMAAETGASHHWAGFNVKLAADGLREAAAMTTQIAG 121 Query: 131 QLLPS-APGRLSIAKRAPLGVIGVISPFNFPLILTLRTVAPALAAGNAVVVKPDVRTPVS 189 +++PS PG L++ R P GV+ I+P+N P+IL +R +A LA GN VV K P + Sbjct: 122 EVIPSDVPGNLAMGVRQPAGVVLGIAPWNAPVILGVRAIALPLACGNTVVFKGSEICPAT 181 Query: 190 GGFMLARIFEEAGLPAGLLHVLPGGAE----AGEALVTAPQVPMIAFTGSPAVGRRIGEL 245 G ++A + +EAGLP G+++ + E EA++ P V + FTGS VGR I Sbjct: 182 HGLIIAAL-QEAGLPPGVVNFVTNAPEDAAAIVEAIIAEPAVRRVNFTGSTRVGRIIAAQ 240 Query: 246 AGRHLKKVSLELGGANALVILDDADLDVAASNAAWGAWLHQGQICMAANRILVHASQAAG 305 RHLK LELGG ++L DADLD A AA+GA+ + GQICM+ RI+V S A Sbjct: 241 CARHLKPAVLELGGKAPFIVLHDADLDAAVEAAAFGAFANSGQICMSTERIIVDDSVADE 300 Query: 306 LLERLVDKARHLPVGDGASGQVALGPLIDARQLQRVHGLVQDSVAAGARLLAGGSYEGLF 365 + RL KA LP+GD G V LG ++D ++R + L+ D++A GA L+ GG Sbjct: 301 FVRRLAAKAASLPLGDPRQGPVVLGSVVDMATVERCNALIDDALAKGATLVCGGRASDTL 360 Query: 366 YQPTVLSGVRPGMRVFDEEIFGPVASVITVDSEEEAITLANQNQGGLAAGVISGSLARAL 425 T+L V M ++ EE FGPV ++ V +E AI AN N+ GL++ + S +ARAL Sbjct: 361 MPATLLDHVSANMLIYREESFGPVKPIVRVAGDEAAIACANDNEYGLSSALFSRDVARAL 420 Query: 426 RVAGRLRQGMVHVNDQTVNDECVNPFGGPGIAGNGTSVGGPADWEEYTQWKWITVKEQAT 485 ++ R+ G+ H+N TV+DE PFGG +G G GG A E+T+ +W+TV+ Sbjct: 421 ALSRRIESGICHINGPTVHDEAQMPFGGVKSSGFG-HFGGKAGIAEFTELRWVTVQTTPR 479 Query: 486 PYPF 489 YPF Sbjct: 480 HYPF 483 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 253 bits (647), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 169/461 (36%), Positives = 242/461 (52%), Gaps = 10/461 (2%) Query: 8 LLDPARWQRHLF-RGRWEETAHGT--TVIEPATGAPLTEVGLAGADDVAISVQAAVAAQA 64 L DPA ++ F G+W + G V PA G L V L GA + +++AA AA Sbjct: 4 LKDPALLRQQAFVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAWP 63 Query: 65 EWLAVPPRERAEIFRRAGNLFQQHFDELARMVARETGGVLFKGEHEVREAITLCHLAAGM 124 W +ER+ I RR L + D+LA ++ E G L + E+ A + A Sbjct: 64 AWKKKTAKERSAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEE 123 Query: 125 PLQAQGQLLPS-APGRLSIAKRAPLGVIGVISPFNFPLILTLRTVAPALAAGNAVVVKPD 183 + G +PS +P + + +GV I+P+NFP + R PALAAG +V+KP Sbjct: 124 GKRTYGDTIPSPSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPA 183 Query: 184 VRTPVSGGFMLARIFEEAGLPAGLLHVLPGGAEA-GEALVTAPQVPMIAFTGSPAVGRRI 242 TP S LA + E AG+PAG+ V+ G + G + + P V I+FTGS VG+ + Sbjct: 184 EATPFSA-LALAVLAERAGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKLL 242 Query: 243 GELAGRHLKKVSLELGGANALVILDDADLDVAASNAAWGAWLHQGQICMAANRILVHASQ 302 + + +KK+SLELGG ++ DDADLD A A + + GQ C+ ANRI V Sbjct: 243 MQQSASSIKKLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGV 302 Query: 303 AAGLLERLVDKARHLPVGDGASGQVALGPLIDARQLQRVHGLVQDSVAAGARLLAGGSYE 362 +LV+ + VG G V GPLI+ + +Q+V V D+VA GAR+L GG Sbjct: 303 YDAFAAKLVEAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKRH 362 Query: 363 GL---FYQPTVLSGVRPGMRVFDEEIFGPVASVITVDSEEEAITLANQNQGGLAAGVISG 419 L F++PTVL+ V P M+V EE FGP+A + ++EE + LAN + GLA+ S Sbjct: 363 ALGHSFFEPTVLADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYSR 422 Query: 420 SLARALRVAGRLRQGMVHVNDQTVNDECVNPFGGPGIAGNG 460 + R RVA L GMV +N +++E V PFGG +G G Sbjct: 423 DIGRIWRVAEGLESGMVGINTGLISNE-VAPFGGVKQSGLG 462 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 244 bits (623), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 169/424 (39%), Positives = 238/424 (56%), Gaps = 12/424 (2%) Query: 66 WLAVPPRERAEIFRRAGNLFQQHFDELARMVARETGGVLFKGEH-EVREAITLCHLAAGM 124 W + +R ++ + L ++ D LA M AR G + +V AI+ AAG Sbjct: 60 WSKLSGLQRGQLLHKLAALVERDTDLLADMDARAIGRSPMEPRMMDVPNAISHLRAAAGW 119 Query: 125 PLQAQGQLLPSA----PGRLSIAKRAPLGVIGVISPFNFPLILTLRTVAPALAAGNAVVV 180 Q +G+ +P+A LS R P+GV+G I P+N PL++T VA LAAG VV+ Sbjct: 120 ANQMEGRTIPTAGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVI 179 Query: 181 KPDVRTPVSGGFMLARIFEEAGLPAGLLHVLPG-GAEAGEALVTAPQVPMIAFTGSPAVG 239 KP TP S LAR+ +EAG P G+++V+ G G G AL P V I+FTGSP G Sbjct: 180 KPAEETPQSA-LHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEAG 238 Query: 240 RRIGELAGRHLKKVSLELGGANALVILDDADLDVAASNAAWGAWLHQGQICMAANRILVH 299 R I +AG K+V+LELGG + ++ DDA + A G +++QGQ+C A +RILV Sbjct: 239 RAIQRIAGEQFKRVTLELGGKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQ 298 Query: 300 ASQAAGLLERLVDKARHLPVGDGASGQVALGPLIDARQLQRVHGLVQDSVAAGARLLAGG 359 S A + L + A + VGD + V +GP+ Q +RV+ +Q + GA LLAGG Sbjct: 299 RSLAQRFAKALAEAAAGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGG 358 Query: 360 SY---EGLFYQPTVLSGVRPGMRVFDEEIFGPVASVITVDSEEEAITLANQNQGGLAAGV 416 +G F QPT+ + GM + EEIFGPV ++I+ D+EEEAI LAN ++ GLAA V Sbjct: 359 VSRPDKGWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGLAATV 418 Query: 417 ISGSLARALRVAGRLRQGMVHVNDQTVNDECVNPFGGPGIAGNGTSVGGPADWEEYTQWK 476 + +LARA RVA ++ G V VN + D + P+GG +G G GG A YT+ K Sbjct: 419 WTTNLARAHRVAAGVKVGAVGVNCWSPLDANL-PWGGIKDSGMGRE-GGLAGALAYTEEK 476 Query: 477 WITV 480 +TV Sbjct: 477 TVTV 480 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 244 bits (622), Expect = 1e-65, Method: Compositional matrix adjust. Identities = 156/469 (33%), Positives = 257/469 (54%), Gaps = 10/469 (2%) Query: 18 LFRGRWEETAHGTT--VIEPATGAPLTEVGLAGADDVAISVQAAVAAQAEWLAVPPRERA 75 G W +A G T VI PAT + ++ A D+ +++AA W + ER+ Sbjct: 8 FINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSAFERS 67 Query: 76 EIFRRAGNLFQQHFDELARMVARETGGVLFKGEHEVREAITLCHLAAGMPLQAQGQLLPS 135 +I RRA +L ++ +E+AR++ E G L + + E A A + G+L+P+ Sbjct: 68 KIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRLVPA 127 Query: 136 -APGRLSIAKRAPLGVIGVISPFNFPLILTLRTVAPALAAGNAVVVKPDVRTPVSGGFML 194 PG + + P+G + +P+NFPL +R ++ ALAAG +++VK TP S ++ Sbjct: 128 RVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAELI 187 Query: 195 ARIFEEAGLPAGLLHVLPG-GAEAGEALVTAPQVPMIAFTGSPAVGRRIGELAGRHLKKV 253 R + +AG+PAG+++++ G AE E L+ P + I+FTGS VG+++ LAG+H+K+ Sbjct: 188 -RAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALAGQHMKRA 246 Query: 254 SLELGGANALVILDDADLDVAASNAAWGAWLHQGQICMAANRILVHASQAAGLLERLVDK 313 ++ELGG ++ DDAD+DVAA A + + GQ+C++ R LV +++ V Sbjct: 247 TMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKFVTY 306 Query: 314 ARHLPVGDGASGQVALGPLIDARQLQRVHGLVQDSVAAGARLLAGGS---YEGLFYQPTV 370 A L VG+G + +GP+ + R++ + L+ D+V+ GA+L GG +G F+QPTV Sbjct: 307 AEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKGYFFQPTV 366 Query: 371 LSGVRPGMRVFDEEIFGPVASVITVDSEEEAITLANQNQGGLAAGVISGSLARALRVAGR 430 L+ + R +EE FGP+A + + ++ +E IT AN+ GLAA + S +A R Sbjct: 367 LTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTKHALAAR 426 Query: 431 LRQGMVHVNDQTVNDECVNPFGGPGIAGNGTSVGGPADWEEYTQWKWIT 479 + GM+ +N + + PFGG +G G S GG E Y K +T Sbjct: 427 VESGMLTINHLGLALPEL-PFGGIKDSGYG-SEGGTEAIEAYINTKLVT 473 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,671,681 Number of extensions: 76160 Number of successful extensions: 345 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 276 Number of HSP's successfully gapped: 21 Length of query: 489 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 400 Effective length of database: 1,198,455 Effective search space: 479382000 Effective search space used: 479382000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)