BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0371.p (474 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313868|ref|YP_003777960.1| major facilitator superfamily p... 694 0.0 gi|300310615|ref|YP_003774707.1| major facilitator superfamily p... 134 1e-32 gi|300310666|ref|YP_003774758.1| major facilitator superfamily p... 112 5e-26 gi|300310645|ref|YP_003774737.1| major facilitator superfamily b... 99 9e-22 gi|300312616|ref|YP_003776708.1| major facilitator superfamily p... 80 4e-16 >gi|300313868|ref|YP_003777960.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 478 Score = 694 bits (1790), Expect = 0.0, Method: Compositional matrix adjust. Identities = 399/475 (84%), Positives = 430/475 (90%), Gaps = 1/475 (0%) Query: 1 MIATPSATIPARLDRLPWSRWHWRVVIALGVAWVLDGLEVTLVGSLGSVLERSDTLALDA 60 M + +A++PARLDRLPWSRWHWRVVIALGVAWVLDGLEVTLVGS+GSVLER DTL LDA Sbjct: 1 MPLSAAASLPARLDRLPWSRWHWRVVIALGVAWVLDGLEVTLVGSIGSVLERPDTLGLDA 60 Query: 61 AQVGWTGSLYIAGTVLGALVFGRLTDRLGRKRLFMLTLMLYMAATLATAFAQDFLFLALC 120 AQVGWTGSLYIAG V+GALVFGRLTDRLGRK+LFM+TL LYMAATLA+AFA DFL+ ALC Sbjct: 61 AQVGWTGSLYIAGAVVGALVFGRLTDRLGRKKLFMITLALYMAATLASAFATDFLWFALC 120 Query: 121 RFVTGLGIGGEYAAINSAIDELVPARVRGRVNLIINASFWIGAALGAGLSLVILDPQVMG 180 R TGLGIGGEYAAINSAIDELVPARVRGRVNL+INASFWIGAALGAGLSLVILD +V+G Sbjct: 121 RLATGLGIGGEYAAINSAIDELVPARVRGRVNLVINASFWIGAALGAGLSLVILDERVLG 180 Query: 181 AALGWRACFLLGAVLAVAVLLVRRHVPESPRWLLMHGRHAEAEAVVRRIEAEVEQQHGPL 240 +GWR CFLLGAVLAVA++LVRRHVPESPRWL MHG HA AEAVVR IEA+V +HG L Sbjct: 181 PVMGWRVCFLLGAVLAVAIVLVRRHVPESPRWLAMHGHHARAEAVVREIEAQVMAEHGTL 240 Query: 241 PAVEL-APSASTQSGTAGSGMLPWREIAHVLLHRYRQRSVMVALMMVAQAFVYNAIFFTY 299 PA+ AP S++S G+L WREIA VLLH YR RS+MVALMMVAQAFVYNAIFFTY Sbjct: 241 PALPTAAPGESSRSPGQAVGLLSWREIAQVLLHHYRARSLMVALMMVAQAFVYNAIFFTY 300 Query: 300 SLVLTRFFQVPDHRVALYIFPFALGNVLGPLLLGHLFDSIGRRRMIAATYLLSGAGLAAT 359 SLVLTRFFQVPD RVALY+FPFALGNVLGPLLLGHLFDSIGRRRMIAATYLL+G GLAAT Sbjct: 301 SLVLTRFFQVPDTRVALYLFPFALGNVLGPLLLGHLFDSIGRRRMIAATYLLAGVGLAAT 360 Query: 360 AWAFNAGMLDARSLALCWSAVFFVASAAASSAYLTASEVFPLRMRAMAISIFYAIGTGVG 419 A AFNAG LDA LALCWSAVFFVASAAASSAYLTASEVFPL+MRAMAI+IFYAIGTGVG Sbjct: 361 AAAFNAGWLDALGLALCWSAVFFVASAAASSAYLTASEVFPLQMRAMAIAIFYAIGTGVG 420 Query: 420 GFVAPVLLGWLIASGDRHAVAAGYALGACLALLAGVCALRFAVDAERRTLEEVAE 474 GF+AP LLGWLIASGDR AVAAGYA+GA LALLAG+CALR+AVDAERRTLEEVAE Sbjct: 421 GFIAPALLGWLIASGDRQAVAAGYAVGAVLALLAGLCALRWAVDAERRTLEEVAE 475 >gi|300310615|ref|YP_003774707.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 478 Score = 134 bits (337), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 146/479 (30%), Positives = 222/479 (46%), Gaps = 50/479 (10%) Query: 12 RLDRLPWSRWHWRVVIALGVAWVLDGLEVTLVGSLGSVLERSDTL-ALDAAQVGWTGSLY 70 R++ +P+SRWH + I +G A + D SL VL L L AAQ+G + Sbjct: 32 RIENVPFSRWHTKARIIMGSATLFDAFHAL---SLAFVLPVLVGLWQLSAAQIGLLIASG 88 Query: 71 IAGTVLGALVFGRLTDRLGRKRLFMLTLMLYMAATLATAFAQDFLFLALCRFVTGLGIGG 130 G V+GAL FG + +R GR +T+ L ++ A F L LCRF+ G+G+GG Sbjct: 89 YLGQVIGALFFGWMAERYGRVPSASVTVGLMSVLSIVCALTGSFHMLFLCRFLQGIGVGG 148 Query: 131 EYAAINSAIDELVPARVRGRVNLIINASFWIGAALGAGLSLVILDPQVMGAALGWRACFL 190 E + I+EL A RG+ ++ F +G L A L P+ GW FL Sbjct: 149 EVPVAATYINELSQAHGRGKFFVLYELIFPLG-LLAAAQVGAFLVPR-----FGWEYMFL 202 Query: 191 LGAVLAVAVLLVRRHVPESPRWLLMHGRHAEAEAVVRRIEAEVEQQHGPLPAVELAPSAS 250 +G + + V L+ +PESPRWL+ GR +AE ++R +EA +++ + A Sbjct: 203 VGGIPGILVALLITRLPESPRWLIARGRLDDAEKLIRTLEASTPKRNLDAQTDKQAIEQR 262 Query: 251 TQSGTAGSGML---PWREIAHVLLHRYRQRSVMVALMMVAQAFVYNAIFFTYSLVLTRFF 307 + AG+G W+E+ + YR R+++V L+ + FV N I + + Sbjct: 263 INALAAGAGKRQRGSWKELFSPV---YRGRTIIVWLLWASSYFVANGINNWLPSLYKTVY 319 Query: 308 QVPDH---RVALYIFPFALGNVLGPLLLGHLFDSIGRRRMIAATYLLSGAGLAATAWAFN 364 +P R+A + VLG L L D +GRRR A+++LSGA LA Sbjct: 320 HLPLQESLRMASVSNVLSACAVLGCAL---LIDRVGRRRWAIASFILSGALLALLG---- 372 Query: 365 AGMLDARSLALCWSAVFFVASAAASSA------YLTASEVFPLRMRA----MAISIFYAI 414 +L A S WS +F +SA A YL E++P RMRA +A S A Sbjct: 373 --VLKAES---AWSVMFLASSAYAVMGTTTVLLYLYTPEIYPTRMRAIGTGLATSWLRAA 427 Query: 415 GTGVGGFVAPVLLGWLIASGDRHAVAAGYALGACLALLAGVCALRFAVDAERRTLEEVA 473 V VL G H +A + + A + +L + A R ++ R+LEE++ Sbjct: 428 SAAAPAMVGVVLAG--------HGIATVFLMFAGVTVLGLLAAWRM-IETTNRSLEEIS 477 >gi|300310666|ref|YP_003774758.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 438 Score = 112 bits (281), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 73/226 (32%), Positives = 123/226 (54%), Gaps = 9/226 (3%) Query: 20 RWHWRVVIALGVAWVLDGLEVTLVGSLGSVLERSDTLALDAAQVGWTGSLYIAGTVLGAL 79 ++ +R+ + G+ W+ D ++V +G + + T L Q TG+++ G +LGA Sbjct: 19 KFQYRLFVIFGLVWLADAMQVLSIGFSAPTIAK--TFGLTVPQALQTGTMFFVGMLLGAF 76 Query: 80 VFGRLTDRLGRKRLFMLTLMLYMAATLATAFAQDFLFLALCRFVTGLGIGGEYAAINSAI 139 VFGRL DR+GR+ + M +++ +A+AFA DF +L R +TG+G+GG + + Sbjct: 77 VFGRLADRIGRRPVLMGAVVIDACFGVASAFAPDFTWLLALRLLTGIGVGGTLPVDYTMM 136 Query: 140 DELVPARVRGRVNLIINASFW-IGAALGAGLSLVILDPQVMGAALGWRACFLLGAVLAVA 198 E +P+ RGR L++ SFW IG A L+LV V WR F + + A+ Sbjct: 137 AEFLPSARRGR-WLVMLESFWAIGTICLAVLALV----AVSWGNDAWRVIFFVTGLPALV 191 Query: 199 VLLVRRHVPESPRWLLMHGRHAEAEAVVRRIEAEVEQQHGPLPAVE 244 +++R +VPESP +L G+ A V+ R+ A+V P+P ++ Sbjct: 192 GVVLRFYVPESPMYLNRSGKSDAARQVLERV-AKVNGSSTPIPPLQ 236 >gi|300310645|ref|YP_003774737.1| major facilitator superfamily benzoate permease [Herbaspirillum seropedicae SmR1] Length = 455 Score = 98.6 bits (244), Expect = 9e-22, Method: Compositional matrix adjust. Identities = 63/220 (28%), Positives = 115/220 (52%), Gaps = 8/220 (3%) Query: 13 LDRLPWSRWHWRVVIALGVAWVLDGLEVTLVGSLGSVLERSDTLALDAAQVGWTGSLYIA 72 +D ++++HW V+ + V DG ++ + G++ VL + +L Q G GS + Sbjct: 10 IDGARFTKFHWMVMCLCALLLVFDGYDLFIYGAVLPVLMKE--WSLSPVQAGALGSYALF 67 Query: 73 GTVLGALVFGRLTDRLGRKRLFMLTLMLYMAATLATAFAQDFLFLALCRFVTGLGIGGEY 132 G + GA +FG L DR+GRK+ ++ +L+ +T + FA + RF+ GLG GG Sbjct: 68 GMMFGAFIFGPLADRIGRKKGVAISFVLFSVSTFLSGFATSPTEFGVYRFLAGLGCGGLM 127 Query: 133 AAINSAIDELVPARVRGRVNLIINASFWIGAALGAGLSLVILDPQVMGAALGWRACFLLG 192 + ++E P R+R + I+ + + +G L AGL + +L GW++ F Sbjct: 128 PNAVALMNEYAPKRLRSTLVAIMFSGYSLGGVLCAGLGIWMLP------NFGWQSMFFAA 181 Query: 193 AVLAVAVLLVRRHVPESPRWLLMHGRHAEAEAVVRRIEAE 232 AV V + ++ +PES +LL GR +A A++ +++ + Sbjct: 182 AVPLVLLPVILWKLPESVGFLLRQGRLEQARAMLTQVDPQ 221 >gi|300312616|ref|YP_003776708.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 414 Score = 80.1 bits (196), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 97/386 (25%), Positives = 159/386 (41%), Gaps = 63/386 (16%) Query: 57 ALDAAQVGWTGSLYIAGTVLGALVFGRLTDRLGRKRLFMLTLMLYMAATLATAFAQDFLF 116 ++ A+ G+ + + + +G G L DR GR R+ T++ + T + F F Sbjct: 43 SMSKAEAGYIATGALLTSAIGGWAAGVLADRYGRVRVLQWTVLWFAFFTFLSGFTTSFGQ 102 Query: 117 LALCRFVTGLGIGGEYAAINSAIDELVPARVRGRVNLIINASF---WIGAALGAGLSLVI 173 L R + G G+GGE+A + I E + AR RG+ + ++ +S+ W G+AL + Sbjct: 103 LFFTRAMQGFGMGGEWAVGSVLIAETISARHRGKASGLVQSSWAVGWAGSALAFWGVYAV 162 Query: 174 LDPQVMGAALGWRACFLLGAVLAVAVLLVRRHVPESPRWLLMHGRHAEAEAVVRRIEAEV 233 L P+ W+ F +G + A+ L +RR + E Sbjct: 163 LPPET-----AWKVLFWIGVLPALLTLYIRRAISE------------------------- 192 Query: 234 EQQHGPLPAVELAPSAS-TQSGTAGSGMLPWREIAHVLLHRYRQRSVMVALMMVAQAFVY 292 PAV LA A +SG G+ + + HR R+ ++A ++ Sbjct: 193 -------PAVYLATQARLRESGQRGNFLR--------IFHRDLLRTTLLASLLATGM--- 234 Query: 293 NAIFFTYSLVLTRFFQVPDHRVAL----YIFPFALGNVLGPLLLGHLFDSIGRRRMIAAT 348 +++ + L F + H L Y+ G+ G L L D+IGRRR Sbjct: 235 QGAYYSVTTWLPTFLKTERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTIGRRRCFM-- 292 Query: 349 YLLSGAGLAATAWAFNAGMLDARSLALCWSAVFFVASA-AASSAYLTASEVFPLRMRAMA 407 L + G+ + DA LAL + FF++ + AYL SE+FP +R Sbjct: 293 -LFAAMGIVLVLSYTQMIITDAWMLALGFVLGFFLSGIFSGMGAYL--SELFPSDVRGSG 349 Query: 408 ISIFYAIGTGVGGFVAPVLLGWLIAS 433 Y G VG + P L+G+L S Sbjct: 350 QGFCYNFGRAVGA-ICPALVGYLSNS 374 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.139 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,232,531 Number of extensions: 45614 Number of successful extensions: 424 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 407 Number of HSP's successfully gapped: 20 Length of query: 474 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 385 Effective length of database: 1,198,455 Effective search space: 461405175 Effective search space used: 461405175 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)