BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0362.p (295 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogen... 160 9e-41 gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogen... 124 9e-30 gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenas... 124 9e-30 gi|300311332|ref|YP_003775424.1| dehydrogenase [Herbaspirillum s... 87 1e-18 >gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 297 Score = 160 bits (406), Expect = 9e-41, Method: Compositional matrix adjust. Identities = 109/288 (37%), Positives = 158/288 (54%), Gaps = 10/288 (3%) Query: 2 AKVGFIGLGIMGAPMADNLQKGGHKLYLHDQ-KNPPAPLLE-GGATVCTSGAEVARRADI 59 A + FIGLG MG PMA NL K G + HD K L+E GG T S A VA+ AD+ Sbjct: 3 ANIVFIGLGNMGLPMAQNLVKAGFSVSGHDLVKASVDKLVEVGGKTEADSMAAVAK-ADV 61 Query: 60 VIVMVPDTPHVQAVLFGEKGVAEGLSPGKIVVDMSSISPIATKEFAQKINALGCQYLDAP 119 VI M+P + HV+++ GE GV PG +++D S+I+P A ++ A A G + LDAP Sbjct: 62 VITMLPASRHVESLYLGEAGVLASAKPGTLLIDCSTIAPEAARKVAAAAKARGFEMLDAP 121 Query: 120 VSGGEVGAKAASLTIMVGGSESAFNDVKPLFELMGKNITLVGGNGDGQTTKVANQIIVAL 179 VSGG GA A +LT MVGGS+ AF +P + MGK I G +G GQT KV N +++ + Sbjct: 122 VSGGTNGATAGTLTFMVGGSKEAFEAAQPYLQKMGKAIYHAGESGSGQTVKVCNNMLLGI 181 Query: 180 NIQAVAEALLFASKAGADPARVRQALMGGFASSRILEVHG-------ERMVKRTFAPGFR 232 + +EA+ G DP + + + + LEV+ + +A GF Sbjct: 182 LMIGTSEAIRLGMANGMDPKVLSEIMSKSSGRNWTLEVYNPCPGVMETAPASKGYAGGFG 241 Query: 233 IELHQKDLNLALQGAKALGISLPNTAAAQELMNACAANGLAGMDHSAL 280 ++L KDL LA++ A A ++P A A+ L + + NG +D S++ Sbjct: 242 VDLMLKDLGLAVENALATNCAIPLGATARNLYDIHSMNGAGKLDFSSI 289 >gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 288 Score = 124 bits (311), Expect = 9e-30, Method: Compositional matrix adjust. Identities = 81/246 (32%), Positives = 140/246 (56%), Gaps = 2/246 (0%) Query: 1 MAKVGFIGLGIMGAPMADNLQKGGHKLYLHD-QKNPPAPLLEGGATVCTSGAEVARRADI 59 M K+G IGLG MG MA +LQ+GG+++ +D Q++ L + G S A +A ++ Sbjct: 1 MKKIGVIGLGNMGRGMAFSLQRGGYEVLGYDAQESTRTALAQQGLHCVDSIAALAPAVEM 60 Query: 60 VIVMVPDTPHVQAVLFGEKGVAEGLSPGKIVVDMSSISPIATKEFAQKINALGCQYLDAP 119 V++ +P + V+ V+ GE G+ + +PG ++VD ++ P +T+ A ++ A G + +DAP Sbjct: 61 VVLSLPTSDIVEQVVEGEGGLLQHGAPGLVIVDTTTADPNSTRRLAARLQAAGMELVDAP 120 Query: 120 VSGGEVGAKAASLTIMVGGSESAFNDVKPLFELMGKNITLVGGNGDGQTTKVANQIIVAL 179 VSGG GA A++ +++GGS ++P+ +M VG G G K+ N ++ Sbjct: 121 VSGGPKGAATATMGMVIGGSAETVARIEPVLAVMSAKRVHVGPVGAGHVAKLLNNLVTGT 180 Query: 180 NIQAVAEALLFASKAGADPARVRQALMGGFASSRILEV-HGERMVKRTFAPGFRIELHQK 238 ++ EA A+ AG D ++ Q L G A+S+++EV + + F GF ++L +K Sbjct: 181 HLLIAGEAARIAAAAGLDARQIFQGLAAGSANSKVIEVFYPTWIFNEAFDSGFTMKLMRK 240 Query: 239 DLNLAL 244 DL LA+ Sbjct: 241 DLRLAM 246 >gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 298 Score = 124 bits (311), Expect = 9e-30, Method: Compositional matrix adjust. Identities = 80/281 (28%), Positives = 138/281 (49%), Gaps = 2/281 (0%) Query: 4 VGFIGLGIMGAPMADNLQKGGHKLYLHD-QKNPPAPLLEGGATVCTSGAEVARRADIVIV 62 VG IGLG MG +A +L + G ++ D +K G C + AE+A + D+VI Sbjct: 5 VGVIGLGAMGYGVASSLLRAGFNVHACDVRKEVLDKFAAAGGVACANPAELAGKVDVVIT 64 Query: 63 MVPDTPHVQAVLFGEKGVAEGLSPGKIVVDMSSISPIATKEFAQKINALGCQYLDAPVSG 122 +V + + VLFG+ G + PG +V+ ++++P E +++ G LDAPVSG Sbjct: 65 LVVNAAQTETVLFGDNGAVSAMKPGSVVISSATVAPDFAIELGKRLVEKGLLLLDAPVSG 124 Query: 123 GEVGAKAASLTIMVGGSESAFNDVKPLFELM-GKNITLVGGNGDGQTTKVANQIIVALNI 181 G A + +T+M G + ++ + M GK L +G G K+ NQ++ ++I Sbjct: 125 GAARAASGEMTMMTSGPAEVYAKIEDVLAGMAGKVYRLGDTHGIGSKVKIINQLLAGVHI 184 Query: 182 QAVAEALLFASKAGADPARVRQALMGGFASSRILEVHGERMVKRTFAPGFRIELHQKDLN 241 A AEA+ + G +P + + + +S + E ++ + P +++ KDL Sbjct: 185 AASAEAMALGLREGVNPDALYEVITHSAGNSWMFENRVPHILSGDYTPLSAVDIFVKDLG 244 Query: 242 LALQGAKALGISLPNTAAAQELMNACAANGLAGMDHSALCR 282 L L A+ LP +AAA ++ + G G D SA+ + Sbjct: 245 LVLDTARRSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVIK 285 >gi|300311332|ref|YP_003775424.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 292 Score = 87.4 bits (215), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 90/287 (31%), Positives = 142/287 (49%), Gaps = 10/287 (3%) Query: 3 KVGFIGLGIMGAPMADNLQKGGHKLYLHDQKNPPAPL---LEGGATVCTSGAEVARRADI 59 K+ F+GLG MG PMA NL + G+ + ++ PAP+ GA + AE A AD+ Sbjct: 2 KIAFLGLGTMGFPMAANLVRAGYAVRAWNRS--PAPVERLAALGAQAAATPAEAAADADV 59 Query: 60 VIVMVPDTPHVQAVLFGEKGVAEGLSPGKIVVDMSSISPIATKEFAQKINALGCQYLDAP 119 +I M+ D + L +A L PG I V+M++IS A A G Y+ AP Sbjct: 60 LISMLADDDATETSLLDAGALAA-LRPGAIHVNMATISVALALRLAGLHQARGVAYVAAP 118 Query: 120 VSGGEVGAKAASLTIMVGGSESAFNDVKPLFELMGKNITLVGGNGD-GQTTKVANQIIVA 178 V G A+A L I+ G E A V+PL +++G+ +G + K+A ++A Sbjct: 119 VLGRVNVAEAGQLNILAAGEEQALAAVQPLLDVLGQKTWRLGRQPEQANAAKLAMNFMIA 178 Query: 179 LNIQAVAEALLFASKAGADPA-RVRQALMGGFASSRILEVHGERMVKRTFAP-GFRIELH 236 I A++EA+ G D A + A FA+ + + +G+ + F P GF++ L Sbjct: 179 SAIGAMSEAVALVQGYGLDKAGFIELATSTAFAAP-VYKGYGQAIADDRFEPAGFKLALG 237 Query: 237 QKDLNLALQGAKALGISLPNTAAAQELMNACAANGLAGMDHSALCRA 283 KD+ LAL+ + + L +A ++L A+G +D +AL RA Sbjct: 238 LKDVRLALEAGEQAHVPLSLASALRDLHIDGLAHGEGHLDWAALSRA 284 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.134 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 932,905 Number of extensions: 38783 Number of successful extensions: 192 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 184 Number of HSP's successfully gapped: 4 Length of query: 295 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 210 Effective length of database: 1,217,395 Effective search space: 255652950 Effective search space used: 255652950 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)