BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0360.p (591 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme I... 261 1e-70 gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbasp... 124 2e-29 gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspi... 111 2e-25 >gi|300311064|ref|YP_003775156.1| acetolactate synthase isozyme III large subunit [Herbaspirillum seropedicae SmR1] Length = 572 Score = 261 bits (666), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 161/546 (29%), Positives = 272/546 (49%), Gaps = 20/546 (3%) Query: 3 KMRAVDAAVHVLVKEGCLQAFGVPGAAINPFYSALKKHGGISHILARHVEGASHMAEGYT 62 ++ D V L +EG FG PG A+ Y A+ K HIL RH + A H A+ Y+ Sbjct: 5 QLTGADILVRCLAEEGVEHVFGYPGGAVLYIYDAIFKQDKFQHILVRHEQAAVHAADAYS 64 Query: 63 RAKAGNIGLCIGTSGPAGTDMITGLYSAQADSIPILCVTGQAPRARLYKEDFQAVDIESI 122 R+ + +G+ I TSGP T+ +TGL +A DSIP++ ++GQ P + ++ FQ D I Sbjct: 65 RS-SQKVGVAIVTSGPGVTNAVTGLATAYMDSIPMVVISGQVPSTAIGQDAFQECDTVGI 123 Query: 123 AKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLIDLPFDVQMAEIDFDPDTYTPLP 182 +P K V++ + ++AF++ +GRPGPVL+D+P D+ M + + Sbjct: 124 TRPCVKHNFLVKDVKDLAETVKKAFYIATTGRPGPVLVDIPKDITMHTCAYAYPKAVEMR 183 Query: 183 VYRPA--ATRAQIEKAFDMLNAAQRPLLTIGGGVVNADAAALAVEFAELTGVPVIPTLMA 240 Y+P QI KA +L +A+RP++ GGG++ A+AA + + G P TLM Sbjct: 184 SYKPVDKGHSGQIRKALQLLLSAERPMIYTGGGIILANAAPELNKLVDRLGYPCTNTLMG 243 Query: 241 WGAIADDHPQMAGMVGLQTSHRYGNATLLASDFVLGIGNRWANRHTGSIDVF-TKGRTFV 299 GA + GM G+ ++ N + D ++ IG R+ +R G+ F + R + Sbjct: 244 LGAYRSSSDKFVGMPGMHGTYE-ANMAMQHCDVLIAIGARFDDRVIGNPKHFASHARKII 302 Query: 300 HVDIEPTQIGRVFGPDYGIVSDAGAALKLFVEVAKEWKTAGRLPDRSAWLAECQQRKKSL 359 H+DI+P+ I + D IV + L+ + +T AW + + +K Sbjct: 303 HIDIDPSSISKRVKVDIPIVGNVKDVLQELLSQLDAAETRQNGAALDAWWKQINEWRKRD 362 Query: 360 QRRTHFNDTPVKPQRVYEEMNRAFGRETCYVATIGLSQIAAAQFLHVYRDRHWINCGQAG 419 + ++ +KPQ V +++ + + +G Q+ AAQ+ + R WIN G G Sbjct: 363 CLKFATSEEFIKPQSVVQKVWEVTKGDAFITSDVGQHQMWAAQYYGFDKPRRWINSGGLG 422 Query: 420 PLGWTIPAALGVVAADPQKKVVAISGDYDFQFMIEELAVGAQFKLPYLHVLVNNSYLGLI 479 +G +P A+GV A+P V I+G+ Q I+ELA Q+ L +L+NN +LG++ Sbjct: 423 TMGVGLPYAMGVQMANPDATVACITGEASIQMCIQELATCKQYHLTPKIILLNNRFLGMV 482 Query: 480 RQAQRGFEMDYCVQLAFENINA-PELNGYGVDHVAVVEGLGCKAIRVTDP----NAIQDA 534 RQ Q ++DY + + +++ P+ N + E G +++ P A+++A Sbjct: 483 RQWQ---QIDYGSRYSESYMDSLPDFN-------KLAESYGHVGMKIEKPGDVDGALREA 532 Query: 535 FAQAQR 540 FA R Sbjct: 533 FAMKDR 538 >gi|300311584|ref|YP_003775676.1| pyruvate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 564 Score = 124 bits (312), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 134/534 (25%), Positives = 214/534 (40%), Gaps = 28/534 (5%) Query: 8 DAAVHVLVKEGCLQAFGVPGAAINPFYSALKKHGGISHILARHVEGASHMAEGYTRAKAG 67 D + L + G Q FGV G A+NP A+++ I I RH EGA+ A G + G Sbjct: 7 DILIDTLYEIGVRQIFGVVGDALNPLTDAIRRDKRIEWIGVRHEEGAALAAAGQAKL-TG 65 Query: 68 NIGLCIGTSGPAGTDMITGLYSAQADSIPILCVTGQAPRARLYKEDFQAVDIESIAKPVT 127 + +C GT+GP ++ GLY A+ D P+L ++G P A + Q E + + V Sbjct: 66 RLAVCCGTTGPGANHLVAGLYEARKDHAPVLAISGGVPAAHRGTDYLQENSPELLFRDVA 125 Query: 128 KWAVTVREPALVPRVFQQAFHLMRSGRPGPVLIDLPFDVQMAEIDFD-PDTYT---PLPV 183 ++ + P P VF QA + R G +++P DV A+ P +T P+ Sbjct: 126 AYSQIITSPDQAPAVFHQAIAQAYAQR-GVAHLNIPADVIGAKTSGSVPSLHTLRERTPL 184 Query: 184 YRPAATRAQIEKAFDMLNAAQRPLLTIGGGVVNADAAALAVEFAELTGVPVIPTLMAWGA 243 PA A I+ A D++NAA + G G A LA A PV+ T Sbjct: 185 SPPA---ADIQAAADLINAAGSVAIFAGNGCRGAIDEVLA--LASKLQAPVMHTFRGKDL 239 Query: 244 IADDHPQMAGMVGLQTSHRYGNATLLASDFVLGIGNRWANRHTGSIDVFTKGRTFVHVDI 303 + DHP G VGL G L A++ +L +G+ + + + +D Sbjct: 240 ASYDHPHWIGGVGL-IGGAPGTDALHAAEVMLMLGSDYPYS-----EFLPTKSKVIQIDE 293 Query: 304 EPTQIGRVFGPDYGIVSDAGAALKLFVEVAKEWKTAGRLPDRSAWLAECQQRKKSLQRRT 363 +GR GI A++ +E E KT G ++ E K Sbjct: 294 RGFVLGRRAPVTLGITGSVAPAVQQLLERV-EGKTDGAFLQQANAHRERWNAKLDSAAEI 352 Query: 364 HFNDTPVKPQRVYEEMNRAFGRETCYVATIGLSQIAAAQFLHVYRDRHWINCGQAGPLGW 423 + +KPQ V ++ + +V G+ + ++ + + +G Sbjct: 353 KRHHKKIKPQSVARAISDLADDDAAFVVDTGVVTLWCGNWIRQRGGQRILASFNNAAVGT 412 Query: 424 TIPAALGVVAADPQKKVVAISGDYDFQFMIEELAVGAQFKLPYLHVLVNNSYLGLIRQAQ 483 ++ A G+ A D +++VV GD F ++ E + +LP V+ NN GL+ Sbjct: 413 SLGQANGIQALDRERQVVVAVGDGGFTMLLGEFMTSVEHRLPVKVVVFNNREWGLVH--- 469 Query: 484 RGFEMDYCVQLAFENINAPELNGYGVDHVAVVEGLGCKAIRVTDPNAIQDAFAQ 537 EM+ AFE P D A G + V P ++ Q Sbjct: 470 --LEMEEAGMPAFEGSEFPN-----PDFAAFARACGGQGFSVRTPEELEGGLQQ 516 >gi|300311743|ref|YP_003775835.1| acetolactate synthase [Herbaspirillum seropedicae SmR1] Length = 625 Score = 111 bits (277), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 133/538 (24%), Positives = 228/538 (42%), Gaps = 41/538 (7%) Query: 48 ARHVEGASHMAEGYTRAKAGNIGLCIGTS-GPAGTDMITGLYSAQADSIPILCVTG---- 102 A + + +H A Y +A + + TS GP T+++T A + +P+L + G Sbjct: 69 AHNEQAMAHSAIAYAKAHLRQRMMAVTTSIGPGATNLLTAAALAHVNRLPVLLLPGDVFV 128 Query: 103 -QAPRARLYK-EDFQ--AVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRS-GRPGP 157 + P L + EDF + + +P+++ + P + +A ++ + GP Sbjct: 129 SRRPDPVLQQLEDFNDPGLSVNDAFRPLSRMFDRICYPEQLLTALPRAIQVLTDPAQCGP 188 Query: 158 VLIDLPFDVQMAEIDFDPDTYTP-LPVYRPAATRAQ-IEKAFDMLNAAQRPLLTIGGGVV 215 V + LP DVQ D+ D ++P + V R A AQ +E A +L A++PLL GGGV+ Sbjct: 189 VTLALPQDVQTMAYDYPLDFFSPRVIVPRAQAPAAQELEDAVALLRQARQPLLIAGGGVL 248 Query: 216 NADAAALAVEFAELTGVPVIPTLMAWGAIADDHPQMAGMVGLQTSHRYGNATLLASDFVL 275 +A FAE GVPV T A+ H G +G+ T NA +D V+ Sbjct: 249 YGNACEHLRRFAEQHGVPVAETQAGKSALPWSHALQMGAIGV-TGSPAANALAQEADVVI 307 Query: 276 GIGNRWANRHTGSIDVFTKGRTFVHVDIEPTQIGRVFGPDYGIVSDAGAALKLFVEVAKE 335 +G R + TGS +F + R +++++ + G + +DAG L+ + Sbjct: 308 AVGTRLQDFTTGSHTLFAQAR-LLNLNVNAMDAHKWRG--LALQADAGLGLEALSHALEG 364 Query: 336 WKTAGRLPDRSAWLAE-CQQRKKSLQRRTHFNDTPVKPQRVYEEMNRAFGRE------TC 388 W++A R+ LA+ ++R + +T V + R+ C Sbjct: 365 WRSAPEWHARAHTLAQDWRERVAQITGQTDTGGRLPYDGEVIGAIQRSVADSPSQDIVVC 424 Query: 389 YVATI--GLSQIAAAQFLHVYRDRHWINCGQAGPLGWTIPAALGVVAADPQKKVVAISGD 446 T+ L ++ A Y + +C +G+ + LGV A PQ++V+ I GD Sbjct: 425 AAGTLPAELHKLWRAGRPGAYHVEYGYSC-----MGYEVAGGLGVKLAQPQREVIVIVGD 479 Query: 447 YDFQFMIEELAVGAQFKLPYLHVLVNNSYLGLIRQAQRGFEMDYCVQLAFENINAPELN- 505 + M ELA + V+++N G I + Q+ C F N+ A L Sbjct: 480 GSYLMMNSELATSVMLGAKLIVVVLDNRGYGCINRLQQA-----CGGAPFNNMLADCLQA 534 Query: 506 GYG---VDHVAVVEGLGCKAIRVTDPNAIQDAFAQAQRWMAEYRVPVVVEIILERVTN 560 G G +D A LG V ++ A +A+ A+ V ++ R T+ Sbjct: 535 GPGAPAIDFAAHARSLGALGENVKTITELEAALQRAR--AADRSYLVCIDTDASRTTD 590 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,945,113 Number of extensions: 85246 Number of successful extensions: 244 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 234 Number of HSP's successfully gapped: 4 Length of query: 591 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 500 Effective length of database: 1,188,985 Effective search space: 594492500 Effective search space used: 594492500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)