BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0349.p (519 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312564|ref|YP_003776656.1| outer membrane multidrug resist... 876 0.0 gi|300312015|ref|YP_003776107.1| NodT family protein [Herbaspiri... 273 2e-74 gi|300311423|ref|YP_003775515.1| NodT family protein [Herbaspiri... 247 2e-66 gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 174 1e-44 gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 171 1e-43 >gi|300312564|ref|YP_003776656.1| outer membrane multidrug resistance lipoprotein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 876 bits (2264), Expect = 0.0, Method: Compositional matrix adjust. Identities = 459/519 (88%), Positives = 474/519 (91%), Gaps = 4/519 (0%) Query: 1 MNKNSSLPDQRAPYPALRSERPTLPWRRTAAVSAAVLTAALMAGCASFTDLGARAEPKSI 60 MN NSS D P +R W R +V AAVL A LMAGCASF+DLG RA+PKSI Sbjct: 1 MNSNSSTLD----LPIQPPQRSGRAWPRAVSVLAAVLAATLMAGCASFSDLGERAQPKSI 56 Query: 61 DQYQSSQTLAATAVQTAWPSDQWWTVYGDAQLNGLIAEALQSAPSMAVAKARLMKAEGTA 120 D+YQS Q+LAA+AVQ AWPSDQWW VYGDAQLN LI EALQSAPSMAVAKARLMKAEG A Sbjct: 57 DRYQSQQSLAASAVQAAWPSDQWWRVYGDAQLNALIDEALQSAPSMAVAKARLMKAEGAA 116 Query: 121 QQQGAALYPQVSGNASLDRMKQSYNNGVPPDFVPKGYNNETRATLDFSYEIDFWGKNRAA 180 QQQGAALYPQVS NASLDRMKQSYNNGVPPDFVPK YNN TRATLDFSYEIDFWGKNRAA Sbjct: 117 QQQGAALYPQVSANASLDRMKQSYNNGVPPDFVPKDYNNATRATLDFSYEIDFWGKNRAA 176 Query: 181 LAAATSELEASRADAAQARITLATSIASAYADLAQLYAQRDTNEAALKVREETLDLFNQR 240 LAAATSELEASRADAAQARITLATSIASAYA+LAQLYAQRDTNEAALKVR E+LDLFNQR Sbjct: 177 LAAATSELEASRADAAQARITLATSIASAYAELAQLYAQRDTNEAALKVRVESLDLFNQR 236 Query: 241 FTNGLETRGSVKQMEARRAIAQADLKATDESIALQRNKLAALAGAGPDRGLQLARPQIDL 300 FTNGLETRGSVKQMEARRAIAQADLKATDESI LQRNKLAAL GAGPDRGLQL RPQIDL Sbjct: 237 FTNGLETRGSVKQMEARRAIAQADLKATDESIGLQRNKLAALLGAGPDRGLQLTRPQIDL 296 Query: 301 SKPFGLPAQLPVELLGRRPDIVAARLRAEAAGKQIKVARAAFYPNVNLSAYFGFQSLGID 360 S+PF LPAQLPVELLGRRPDIVAARLRAEAAGKQIKVARAAFYPNVNL+AYFGFQSLGID Sbjct: 297 SRPFALPAQLPVELLGRRPDIVAARLRAEAAGKQIKVARAAFYPNVNLTAYFGFQSLGID 356 Query: 361 MLTRSGSDIGSFGPAISLPIFNGGRLRGQFRSASASYDEAVANYDQAVTQALQDVADVGV 420 MLTR+GSDIGS GPAISLPIFNGGRLRGQFRSASASYDEAVANYDQAVTQALQDVADVGV Sbjct: 357 MLTRAGSDIGSIGPAISLPIFNGGRLRGQFRSASASYDEAVANYDQAVTQALQDVADVGV 416 Query: 421 SEKALAGRLQDVQAAADAAEEAYRIVSNRYNGGLNTYLDVLNAQDTLISNLRQLSDLRSR 480 SEKALAGRL DVQAAADAAEEAYRIVSNRYNGGL TYLDVLNAQDTLISNLRQLSDLRSR Sbjct: 417 SEKALAGRLADVQAAADAAEEAYRIVSNRYNGGLATYLDVLNAQDTLISNLRQLSDLRSR 476 Query: 481 LFTLDVALVRALGGGYRAADDSKSQDSATAPHTEQQAKG 519 +FTLDVALVRALGGGYRAADDS SQ+SA AP + QAKG Sbjct: 477 MFTLDVALVRALGGGYRAADDSSSQNSADAPLHDTQAKG 515 >gi|300312015|ref|YP_003776107.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 495 Score = 273 bits (699), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 182/467 (38%), Positives = 266/467 (56%), Gaps = 14/467 (2%) Query: 43 AGCASFTDLGARAEPKSIDQYQSSQTLA---ATAVQTAWPSDQWWTVYGDAQLNGLIAEA 99 +GCASF +G+ + + + ++L+ A WP W +GDAQL L+ +A Sbjct: 27 SGCASFAGIGSDRQVAQAGDFATQRSLSDPNPGAPNGQWPGSDWVRQFGDAQLVALVEQA 86 Query: 100 LQSAPSMAVAKARLMKAEGTAQQQGAALYPQVSGNASLDRMKQSYNNGVPPDFVPKGYNN 159 L S+PS+ A+AR+ A A+ +GA L P V+ AS+ R + S PP + YN Sbjct: 87 LTSSPSLQQARARIAAASALAESRGAPLLPSVNAEASVTRNQFSSTTIYPPPYGGNWYN- 145 Query: 160 ETRATLDFSYEIDFWGKNRAALAAATSELEASRADAAQARITLATSIASAYADLAQLYAQ 219 E +A L+ YE+D W KN+AALA A S +A++A +AR+ L SI + Y+ LA YA Sbjct: 146 EKKAGLNVGYELDLWNKNQAALAQAISSEKAAQASEQEARLALTASIVTVYSQLAAQYAL 205 Query: 220 RDTNEAALKVREETLDLFNQRFTNGLETRGSVKQMEARRAIAQADLKATDESIALQRNKL 279 D ++ + R + +R GL+++ Q A A+A L ++ I L R +L Sbjct: 206 HDILQSTVDQRTSLEKITAERLRTGLDSQIERDQSRTSSADARAQLAQSEGQIVLLRQQL 265 Query: 280 AALAGAGPDRGLQLARPQID-LSKPFGLPAQLPVELLGRRPDIVAARLRAEAAGKQIKVA 338 ALAG GPD GLQLA P + L+ P GLPA+LP+ L+GRRPDIVAAR + EAA + + VA Sbjct: 266 GALAGKGPDYGLQLAPPALQGLATP-GLPAELPLNLMGRRPDIVAARWQVEAASRGVDVA 324 Query: 339 RAAFYPNVNLSAYFGFQSLGIDMLTRSGSDIGSFGPAISLPIFNGGRLRGQFRSASASYD 398 +A FYP++NLSA GF +L + S +FGPAI+LPIF GG LR + ASY+ Sbjct: 325 KARFYPDINLSAMIGFDTLLDSNPFTAASKSIAFGPAITLPIFEGGALRAGLKGEYASYE 384 Query: 399 EAVANYDQAVTQALQDVAD----VGVSEKALAGRLQDVQAAADAAEEAYRIVSNRYNGGL 454 AVA Y++ + A DVA + +E+ L R + +Q AAE AY + RY GL Sbjct: 385 LAVATYNKTLNDAYADVARQIAAIHATERQLPIRSEALQ----AAERAYALARERYRLGL 440 Query: 455 NTYLDVLNAQDTLISNLRQLSDLRSRLFTLDVALVRALGGGYRAADD 501 + L +L+AQ +++ + + L+++ VAL +ALGGG+ A D Sbjct: 441 VSQLTLLSAQTGVLAQRQAMVALQAQRRDQQVALYKALGGGFDAQRD 487 >gi|300311423|ref|YP_003775515.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 487 Score = 247 bits (630), Expect = 2e-66, Method: Compositional matrix adjust. Identities = 181/464 (39%), Positives = 258/464 (55%), Gaps = 3/464 (0%) Query: 42 MAGCASFTDLGARAEPKSIDQYQSSQTLAATAVQTAWPSDQWWTVYGDAQLNGLIAEALQ 101 MAGCA + +A+ + + + Q + A + WP WW YGDAQL+ L+ A+ Sbjct: 21 MAGCADQGGIQPQAQVRDVAVLDAGQAIRQAASEAGWPQRAWWQAYGDAQLDQLVTRAVA 80 Query: 102 SAPSMAVAKARLMKAEGTAQQQGAALYPQVSGNASLDRMKQSYNNGVPPDFVPKGYNNET 161 PSMAVA AR+ +A+ A A P + S+ R S N F K N T Sbjct: 81 GNPSMAVAAARVRQAQSMAVVAHAGELPSAQLDVSVGRKDWSDNVYYGAAFRNKLTWNNT 140 Query: 162 RATLDFSYEIDFWGKNRAALAAATSELEASRADAAQARITLATSIASAYADLAQLYAQRD 221 ATL SY +D W +N+ A A E+ A ADA A++ L ++ Y LA Y D Sbjct: 141 -ATLGLSYNVDLWDRNKNATLRAQDEMHAVAADARAAQLDLEGNVVRTYVQLALQYGLLD 199 Query: 222 TNEAALKVREETLDLFNQRFTNGLETRGSVKQMEARRAIAQADLKATDESIALQRNKLAA 281 EA LK ++ LDL ++RF GL T+ + Q E+ + ++A E+IAL RN+LAA Sbjct: 200 NTEALLKEEQKILDLAHRRFKGGLGTQLEITQAESPLPETRRQIEALHENIALIRNQLAA 259 Query: 282 LAGAGPDRGLQLARPQIDLSKPFGLPAQLPVELLGRRPDIVAARLRAEAAGKQIKVARAA 341 L GAGP + RP + L GLP+ LP +L+GRRPDI AAR R EAA K I VA+AA Sbjct: 260 LLGAGPGAADGVTRPGMQLQTSLGLPSALPADLVGRRPDISAARWRVEAAAKGISVAKAA 319 Query: 342 FYPNVNLSAYFGFQSLGIDMLT-RSGSDI-GSFGPAISLPIFNGGRLRGQFRSASASYDE 399 FYPN+NL A G + G + + S ++ ++GPAISLPIF GGRLRGQ +ASA YD Sbjct: 320 FYPNINLLAGIGPAAAGGGLFSFLSAPNLQTTYGPAISLPIFEGGRLRGQLGAASAGYDI 379 Query: 400 AVANYDQAVTQALQDVADVGVSEKALAGRLQDVQAAADAAEEAYRIVSNRYNGGLNTYLD 459 V +Y+ ++QAL+ ++D V+ +++A + + Q + AA++ I + Y GL YL+ Sbjct: 380 EVEHYNALLSQALKGISDQVVTLQSVARQQEQAQQSVQAAQKNVDIATRAYQRGLTDYLN 439 Query: 460 VLNAQDTLISNLRQLSDLRSRLFTLDVALVRALGGGYRAADDSK 503 VL+AQ + + + L+++ AL ALGGG A +K Sbjct: 440 VLHAQTQWLRQQQIVQQLQAQRLAAYAALQTALGGGIEEAAPAK 483 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 174 bits (441), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 140/426 (32%), Positives = 213/426 (50%), Gaps = 9/426 (2%) Query: 72 TAVQTAWPSDQWWTVYGDAQLNGLIAEALQSAPSMAVAKARLMKAEGTAQQQGAALYPQV 131 A + A P W + D L LI +AL+++ + A R+ +A+ A L+P + Sbjct: 55 VANEAASPPLPWRDYFTDPPLQALITQALENSRDLRTAALRVQEAQALYGIGHADLFPTI 114 Query: 132 SGNASLDRMKQSYN-NGVPPDFVPKGYNNETRATLDFS-YEIDFWGKNRAALAAATSELE 189 A LDR + + N V Y +A L S +EID WG+ R+ AA Sbjct: 115 GAQAGLDRSRTPADLNLTRRPLVGSAY----QAGLGLSSWEIDLWGRLRSLDDAALESAR 170 Query: 190 ASRADAAQARITLATSIASAYADLAQLYAQRDTNEAALKVREETLDLFNQRFTNGLETRG 249 AS+A + ++L +A A+ +LA+L + + + R+E+ +F++R G +R Sbjct: 171 ASQATRSAVALSLMAQVADAWLELAELDERLEIANQTIASRQESFRIFSRRVEVGATSRL 230 Query: 250 SVKQMEARRAIAQADLKATDESIALQRNKLAALAGAGPDRGLQLARPQID-LSKPFGLPA 308 ++ Q+E AQA + A + N L L G+ + AR +D + P L Sbjct: 231 NLTQIETLLTQAQALGAQLQQQRAQRLNALTLLVGSPVVLPVGQARAGLDAVFAP--LQP 288 Query: 309 QLPVELLGRRPDIVAARLRAEAAGKQIKVARAAFYPNVNLSAYFGFQSLGIDMLTRSGSD 368 LP LL RRPD+ AA R AA I ARAAF+P+++L+ G S + L R GS Sbjct: 289 GLPALLLTRRPDVAAAEHRLMAANANIGAARAAFFPSISLTGNVGTASAELSGLFRDGSH 348 Query: 369 IGSFGPAISLPIFNGGRLRGQFRSASASYDEAVANYDQAVTQALQDVADVGVSEKALAGR 428 +F P+I+LPIF GRLR + D AVA Y++ V A ++VAD + + L + Sbjct: 349 AWTFSPSITLPIFTAGRLRNNLALSEVRRDLAVAAYEKTVQTAFREVADALAARQWLTQQ 408 Query: 429 LQDVQAAADAAEEAYRIVSNRYNGGLNTYLDVLNAQDTLISNLRQLSDLRSRLFTLDVAL 488 L+ Q+A E R+ + RY+ G +LDVL+AQ L+S +QL +R L + VAL Sbjct: 409 LEIAQSAVRVQRERARLSALRYDNGAAPFLDVLDAQRDLLSAQQQLVQIRRALLSSSVAL 468 Query: 489 VRALGG 494 ALGG Sbjct: 469 YAALGG 474 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 171 bits (433), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 141/430 (32%), Positives = 212/430 (49%), Gaps = 20/430 (4%) Query: 79 PSDQWWTVYGDAQLNGLIAEALQSAPSMAVAKARLMKAEGTAQQQGAALYPQVSGNASLD 138 P WW +GDAQLN L +AL ++A+A ARL +A GAA PQV+ NA Sbjct: 52 PRGPWWERFGDAQLNALQQQALAGNQTLAIATARLAQARAQLNVTGAAQTPQVNLNARAA 111 Query: 139 RMKQSYNNGVPPDFVPKGYNNETRATL--DF------SYEIDFWGKNRAALAAATSELEA 190 R + S N + YN+ +T+ DF SYE+DF+G+ + ++ AA + E Sbjct: 112 RSRISANRPL------TNYNSPNFSTVQNDFALGASVSYEVDFFGRVQRSVEAAGAAAEQ 165 Query: 191 SRADAAQARITLATSIASAYADLAQLYAQRDTNEAALKVREETLDLFNQRFTNGLETRGS 250 S AD R+ L +A+ Y +L +L + D A+ ++ + L+L R G + Sbjct: 166 SAADLENTRLLLTAELATNYFNLRELDVELDVVRRAIALQRQALELATARHDLGATSGLD 225 Query: 251 VKQMEAR--RAIAQADLKATDESIALQRNKLAALAGAGPDRGLQLARPQIDLSKPFGLPA 308 + Q +A + Q DL A + +A L G P + P + P +P Sbjct: 226 MAQQQALLDSTLTQIDLLGRQR--AQYEHAIATLTGT-PAPAFSIT-PSLTPITPPAIPV 281 Query: 309 QLPVELLGRRPDIVAARLRAEAAGKQIKVARAAFYPNVNLSAYFGFQSLGIDMLTRSGSD 368 +P ++L RRPDI AA AA QI VA AA+YP+ L +G S L + S Sbjct: 282 GVPSDVLERRPDIAAAERAMAAANAQIGVASAAYYPSFMLQPSYGVDSRNWATLFNAPSL 341 Query: 369 IGSFGPAISLPIFNGGRLRGQFRSASASYDEAVANYDQAVTQALQDVADVGVSEKALAGR 428 + S G + S +F+GGRLR + A Y+ VA+Y + V A+Q+V D AL Sbjct: 342 LWSLGVSASQSLFDGGRLRAGVDFSQAGYEATVASYRRTVLTAMQEVEDGITGLAALERA 401 Query: 429 LQDVQAAADAAEEAYRIVSNRYNGGLNTYLDVLNAQDTLISNLRQLSDLRSRLFTLDVAL 488 Q A +A I S+RY GG YLDV+ AQ +L+++ RQ + L + + V L Sbjct: 402 YAQSQLAIASARRVLEIASSRYEGGATPYLDVITAQQSLLNSERQAAQLMGQRLLVSVFL 461 Query: 489 VRALGGGYRA 498 ++ALGG +++ Sbjct: 462 IKALGGDWQS 471 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.127 0.355 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,408,981 Number of extensions: 54812 Number of successful extensions: 287 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 227 Number of HSP's successfully gapped: 20 Length of query: 519 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 430 Effective length of database: 1,198,455 Effective search space: 515335650 Effective search space used: 515335650 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)