BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0348.p (410 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312565|ref|YP_003776657.1| multidrug resistance transmembr... 765 0.0 gi|300312016|ref|YP_003776108.1| multidrug resistance A (translo... 251 9e-68 gi|300312487|ref|YP_003776579.1| multidrug resistance transport ... 137 1e-33 gi|300311270|ref|YP_003775362.1| HlyD family membrane-fusion pro... 61 1e-10 gi|300311421|ref|YP_003775513.1| multidrug resistance efflux pum... 60 4e-10 >gi|300312565|ref|YP_003776657.1| multidrug resistance transmembrane efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 411 Score = 765 bits (1975), Expect = 0.0, Method: Compositional matrix adjust. Identities = 386/405 (95%), Positives = 398/405 (98%) Query: 1 MSHSDSQAAHTLAEETRQRNEAATKAAAAQKRKKLFAIFGGVVAIAAIGYGTYWYLIGSR 60 MSHSDSQAAHTLAEE +QRNEAA+ AAAAQKRKKLF+IFGGVVAIAAIGYG YWYLIGSR Sbjct: 1 MSHSDSQAAHTLAEEAKQRNEAASAAAAAQKRKKLFSIFGGVVAIAAIGYGAYWYLIGSR 60 Query: 61 YVETDNAYTATEVATVTPAINGIVAAVDVVDTQAVKKGDVLVRIDDADAKLAVDQAAADL 120 YVETDNAYTATE+ATVTPAINGIVAAVDVVDTQAVKKGDVLVRIDDADA+LAVDQAAADL Sbjct: 61 YVETDNAYTATEIATVTPAINGIVAAVDVVDTQAVKKGDVLVRIDDADARLAVDQAAADL 120 Query: 121 DRTERKVKGYFANDAGLAAQVLAREAEQKRASAQLLSAQADMKRAEIDLQRREALAKSGS 180 DRTERKVKG+FANDAGLAAQVLAREAEQKRASAQLLSAQAD+KRAEIDLQRREALAKSGS Sbjct: 121 DRTERKVKGFFANDAGLAAQVLAREAEQKRASAQLLSAQADLKRAEIDLQRREALAKSGS 180 Query: 181 VSGEELSNARTALLTAQANLKAAEAAEVQSRANIKATQGAQKASTVLTANTTVDDNPEVV 240 VSGEELSNARTALLTAQANLKAAEAAEVQSRANIKATQGAQKASTVLTANTTVDDNPEVV Sbjct: 181 VSGEELSNARTALLTAQANLKAAEAAEVQSRANIKATQGAQKASTVLTANTTVDDNPEVV 240 Query: 241 LARAKLDQAKLDLERTVLRAPVDGVIARRQVQVGQRVQSGASLLSVVPLQQMHVDANFKE 300 LARAKL+QAKLDLERTVLRAPVDGVIARRQVQVGQRVQSGA+LLSVVPLQQMHVDANFKE Sbjct: 241 LARAKLEQAKLDLERTVLRAPVDGVIARRQVQVGQRVQSGATLLSVVPLQQMHVDANFKE 300 Query: 301 GQLTKVRIGQPVTMKADIYGGSVEYHGVVTGLSGGTGSAFAVIPAQNATGNWIKVVQRLP 360 GQLTKVRIGQPVTMKAD+YGGSVEYHGVVTGLSGGTGSAFAVIPAQNATGNWIKVVQRLP Sbjct: 301 GQLTKVRIGQPVTMKADLYGGSVEYHGVVTGLSGGTGSAFAVIPAQNATGNWIKVVQRLP 360 Query: 361 VRISLDPKELAQRPLSVGLSMVVEIDTRGQMQAGDAQPKSASTET 405 VRISLDPKELAQRPLSVGLSMVVEIDTRGQ+QAGDAQ KSA + Sbjct: 361 VRISLDPKELAQRPLSVGLSMVVEIDTRGQIQAGDAQRKSARNDN 405 >gi|300312016|ref|YP_003776108.1| multidrug resistance A (translocase) transmembrane secretion protein [Herbaspirillum seropedicae SmR1] Length = 406 Score = 251 bits (640), Expect = 9e-68, Method: Compositional matrix adjust. Identities = 159/374 (42%), Positives = 224/374 (59%), Gaps = 28/374 (7%) Query: 31 KRKKLFAIFGGVVAIAAIGYGTYWYLIGSRYVETDNAYTATEVATVTPAINGIVAAVDVV 90 KRK+ + V+ + A+ YW+L + ETD+AY V ++ + G V AV Sbjct: 19 KRKRQLILLTLVLLVIAVACFLYWFLHARFFEETDDAYVGGNVVQISAQVGGTVVAVKAD 78 Query: 91 DTQAVKKGDVLVRIDDADAKLAVDQAAADLDRTERKVKGYFANDAGLAAQVLAREAEQKR 150 DTQ VK G LV +D AD +LA+DQA A L + R+ + F N+ LAA V A Sbjct: 79 DTQVVKAGQQLVALDAADTRLALDQAQAALAQAVRQTRQLFLNNDTLAANVAA------- 131 Query: 151 ASAQLLSAQADMKRAEIDLQRREALAKSGSVSGEELSNARTALLTAQANLKAAEAAEVQS 210 A +++ RA DLQRR+A SG+VS E++S+AR A+ +A A L A AA Sbjct: 132 -------ADSNLARAREDLQRRQAGLSSGAVSQEDVSHARDAVKSAVAALDQARAAA--- 181 Query: 211 RANIKATQGAQKASTVLTANTTVDDNPEVVLARAKLDQAKLDLERTVLRAPVDGVIARRQ 270 A+ LT +T+V ++P V+ A + A L+ R + APV G +++R Sbjct: 182 -----------AANRALTDHTSVTEHPNVLQAATAVRNAYLNYARVNIVAPVSGFVSKRS 230 Query: 271 VQVGQRVQSGASLLSVVPLQQMHVDANFKEGQLTKVRIGQPVTMKADIYGGSVEYHGVVT 330 VQVGQR+ +G L+++VPL+Q+ +DANFKE QL +RIGQPV + AD+YG SV+Y G V Sbjct: 231 VQVGQRIAAGNPLMAIVPLEQIWIDANFKESQLQHIRIGQPVEVIADVYGSSVKYKGTVI 290 Query: 331 GLSGGTGSAFAVIPAQNATGNWIKVVQRLPVRISLDPKELAQRPLSVGLSMVVEIDTRGQ 390 G S GTG AF+++PAQNATGNWIKVVQR+PVRI+LDP+++ PL +GLS +D G Sbjct: 291 GFSAGTGGAFSLLPAQNATGNWIKVVQRVPVRIALDPEQVRAHPLRIGLSTTATVDIHGD 350 Query: 391 MQAGDAQPKSASTE 404 +A +A P + T Sbjct: 351 GRALEAVPTNYQTN 364 >gi|300312487|ref|YP_003776579.1| multidrug resistance transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 397 Score = 137 bits (345), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 70/146 (47%), Positives = 99/146 (67%), Gaps = 2/146 (1%) Query: 242 ARAKLDQAKLDLERTVLRAPVDGVIARRQVQVGQRVQSGASLLSVVPLQQMHVDANFKEG 301 A+A+ D A ++L T +RA VDG I +QV+VGQ V G +++VVP + +V ANFKE Sbjct: 229 AKAQYDAASVNLGYTEIRASVDGRIGDKQVRVGQFVSPGTRMMTVVPTR-FYVSANFKET 287 Query: 302 QLTKVRIGQPVTMKADIYGGSVEYHGVVTGLSGGTGSAFAVIPAQNATGNWIKVVQRLPV 361 Q+ +RIGQP +K D G VE+HG V LS GTG+ F+++P QNATGN+ K+VQR+PV Sbjct: 288 QIGIMRIGQPAIIKVDALPG-VEFHGHVESLSPGTGAQFSLLPPQNATGNFTKIVQRVPV 346 Query: 362 RISLDPKELAQRPLSVGLSMVVEIDT 387 R+++D R GLS+ VE++T Sbjct: 347 RVAIDATPEETRAFVAGLSVTVEVNT 372 >gi|300311270|ref|YP_003775362.1| HlyD family membrane-fusion protein [Herbaspirillum seropedicae SmR1] Length = 358 Score = 61.2 bits (147), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 81/295 (27%), Positives = 137/295 (46%), Gaps = 43/295 (14%) Query: 38 IFGGV--VAIAAIGY-GTYWYL---IGSRYVETDNAYTATEVATVTPAINGIVAAVDVVD 91 +F G+ V +AA+G+ G W+ + V ++ ATEV + + G + + V + Sbjct: 9 LFAGLLLVVLAAVGWTGWAWWRDADAPAGLVSSNGRLEATEV-DIGSRLGGRIRHMLVDE 67 Query: 92 TQAVKKGDVL--VRIDDADAKLAVDQAAADLDRTERKVKGYFANDAGLAAQVLAREAEQK 149 +AV+ G VL +++D A+L +A + +R +G A LA R +EQ+ Sbjct: 68 GEAVQAGQVLAYLQVDSLHAQLREARA-----QQQRASQGVTVAQAQLAM----RRSEQR 118 Query: 150 RASAQLLSAQADMKRAEIDLQRREALAKSGSVSGEELSNARTALLTAQANLKAAEAAEVQ 209 A A + + A ++R + L + +++ +E+ + L A+A L AA+A E Sbjct: 119 IAQALTVQRHKSLSAATRRVERSQPLGRMQALTAQEVDDEENTLAEARAQLDAAQAQE-- 176 Query: 210 SRANIKATQGAQKASTVLTANTTVDDNPEVVLARAKLDQAKL-------DLERTVLRAPV 262 +GA+ A A+ V ARA L+ A+ DLE + +RAP Sbjct: 177 --------EGARAAIRAAEAD--------VASARALLEAAQAATARIEADLEESAIRAPR 220 Query: 263 DGVIARRQVQVGQRVQSGASLLSVVPLQQMHVDANFKEGQLTKVRIGQPVTMKAD 317 DG I R Q G+ V G+ LL+++ L +H+ KVR+GQ V + D Sbjct: 221 DGHIQYRLAQAGEVVSEGSKLLNLIDLSDLHMHFFLPAELAGKVRLGQEVRVVLD 275 >gi|300311421|ref|YP_003775513.1| multidrug resistance efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 324 Score = 59.7 bits (143), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 43/123 (34%), Positives = 66/123 (53%), Gaps = 4/123 (3%) Query: 247 DQAKLDLERTVLRAPVDGVIARRQVQVGQRVQSGASLLSVVPLQQMHVDANFKEGQLTKV 306 D A+L+LERT + +PVDG I V+ G +GA+ ++V+ V A F+E +L + Sbjct: 172 DTARLNLERTKVFSPVDGYITNLNVRRGDYAAAGAAKIAVIDSHSFWVYAYFEETKLPLL 231 Query: 307 RIGQPVTMKADIYGGSVEYH--GVVTGLSGGTGSAFAVIPAQ-NATGNWIKVVQRLPVRI 363 IG ++ I G S+E H + G+ + A N T NW+++ QR+PVRI Sbjct: 232 SIGDRARIQL-INGASMEGHVDSIARGIYDRDNPQSRELTADVNPTFNWVRLAQRIPVRI 290 Query: 364 SLD 366 LD Sbjct: 291 QLD 293 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.126 0.334 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 842,440 Number of extensions: 24309 Number of successful extensions: 162 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 152 Number of HSP's successfully gapped: 6 Length of query: 410 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 323 Effective length of database: 1,207,925 Effective search space: 390159775 Effective search space used: 390159775 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 132 (55.5 bits)