BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0347.p (476 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312566|ref|YP_003776658.1| multidrug resistance protein pr... 929 0.0 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 404 e-113 gi|300312486|ref|YP_003776578.1| major facilitator superfamily p... 199 5e-52 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 119 5e-28 gi|300311040|ref|YP_003775132.1| major facilitator superfamily p... 113 3e-26 >gi|300312566|ref|YP_003776658.1| multidrug resistance protein protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 929 bits (2401), Expect = 0.0, Method: Compositional matrix adjust. Identities = 455/473 (96%), Positives = 467/473 (98%) Query: 4 NVSVSTIAGALGASTSQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFILSMVMFGLFS 63 NVSVSTIAGALGASTSQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFILSMVMFGLFS Sbjct: 53 NVSVSTIAGALGASTSQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFILSMVMFGLFS 112 Query: 64 ALCGLANSLGMLIAFRVMQGLAGGPLMPLSQTLLLRIFPKEKAPAAVGLWAMTTLVAPIA 123 ALCG+ANSLGMLIAFRV+QGLAGGPLMPLSQTLLLRIFPKEKAPAAVGLWAMTTLVAPIA Sbjct: 113 ALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKAPAAVGLWAMTTLVAPIA 172 Query: 124 GPILGGVLCDQYSWPYIFWINVPVALICGYLGWRMLKRFETKTSKLPIDRVGLILMVVWV 183 GPILGGVLCDQYSWPYIFWINVPVALICGY+GWRMLKR+ETK SKLPIDRVGL+LMVVWV Sbjct: 173 GPILGGVLCDQYSWPYIFWINVPVALICGYIGWRMLKRYETKMSKLPIDRVGLVLMVVWV 232 Query: 184 AALQLMLDEGKEKDWFASTEIVLLAVTAVVFFIAFLIWELTEHNPIVDLRVFRHRGFTAS 243 AALQLMLDEGKEKDWFASTEIVLLAVTAVVFFIAFLIWELTE NPIVDLRVFRHRGFTAS Sbjct: 233 AALQLMLDEGKEKDWFASTEIVLLAVTAVVFFIAFLIWELTERNPIVDLRVFRHRGFTAS 292 Query: 244 VTTICLAFGAFFGSVVLTPLWLQGYMGYTATWSGCISALTGILAVLTAPMVAKMSTTVDA 303 VTTICLAFGAFFGSVVLTPLWLQGYMGYTATWSGCISALTGILAV+TAPMVAKMSTT+DA Sbjct: 293 VTTICLAFGAFFGSVVLTPLWLQGYMGYTATWSGCISALTGILAVMTAPMVAKMSTTIDA 352 Query: 304 RKLVCIGVTWLGFITFLRAFNNTDMSFFQIGWPLFLQGIGLPLFFVPLTGLALSSVNEEE 363 RKLVCIGVTWLGFITFLRAFN TDMSFFQIGWPLFLQGIGLPLFFVPLTGLALSSVNEEE Sbjct: 353 RKLVCIGVTWLGFITFLRAFNTTDMSFFQIGWPLFLQGIGLPLFFVPLTGLALSSVNEEE 412 Query: 364 TASAAGLMSFCRTMAGAIATSIVNTSWENDATYFHAELSGLVDRSGDAVNAMMASGMSAE 423 TASAAGLMSFCRTMAGAIATSIVNTSWENDATYFHAELSGLVDR GDA++ MMASGMSAE Sbjct: 413 TASAAGLMSFCRTMAGAIATSIVNTSWENDATYFHAELSGLVDRGGDAIHTMMASGMSAE 472 Query: 424 QATGSLAQTVQNQAVMLATNHIFMMAAVSFSLAALAVWLAPKPTRVADTTSAH 476 QAT SLAQTVQNQAVML+TNHIFMMAAVSFSLAALAVWLAPKPTRVADT++AH Sbjct: 473 QATASLAQTVQNQAVMLSTNHIFMMAAVSFSLAALAVWLAPKPTRVADTSAAH 525 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 404 bits (1037), Expect = e-113, Method: Compositional matrix adjust. Identities = 209/455 (45%), Positives = 292/455 (64%), Gaps = 5/455 (1%) Query: 4 NVSVSTIAGALGASTSQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFILSMVMFGLFS 63 NV++ TI+G LG S +GT+VITS+A A AI++PLTGWL R G VR+F+ S+++F L S Sbjct: 44 NVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRLGAVRLFVTSVLLFVLAS 103 Query: 64 ALCGLANSLGMLIAFRVMQGLAGGPLMPLSQTLLLRIFPKEKAPAAVGLWAMTTLVAPIA 123 LCGLA +L +L+A RV+QGL GP++PLSQ+LLL +PK K+ A+ LW MT +VAP+A Sbjct: 104 WLCGLAPTLPILLAARVLQGLVAGPMVPLSQSLLLGAYPKSKSSFALALWGMTAVVAPVA 163 Query: 124 GPILGGVLCDQYSWPYIFWINVPVALICGYLGWRMLKRFETKTSKLPIDRVGLILMVVWV 183 GP LGG + D Y+WP+IF+IN+PV LI + + + ET T KLPID++GL L+V WV Sbjct: 164 GPALGGWITDSYTWPWIFYINIPVGLIAAGVTLAIYRSRETPTHKLPIDKIGLALLVTWV 223 Query: 184 AALQLMLDEGKEKDWFASTEIVLLAVTAVVFFIAFLIWELTEHNPIVDLRVFRHRGFTAS 243 AA Q+MLD+GK+ DWFAS IV L + A++ F F++WEL +P+VDLR+F R F Sbjct: 224 AAFQIMLDKGKDLDWFASPTIVTLGIIALIAFAYFVVWELNNDHPVVDLRLFGRRNFAGG 283 Query: 244 VTTICLAFGAFFGSVVLTPLWLQGYMGYTATWSGCISALTGILAVLTAPMVAKMSTTVDA 303 I + +G FFG++V+ P WLQ Y+GY + SG +A GI AV+ P++ + VDA Sbjct: 284 TIAISIGYGVFFGNLVILPQWLQQYLGYRSIDSGLSTAPIGIFAVILMPLIGRFLPLVDA 343 Query: 304 RKLVCIGVTWLGFITFLRAFNNTDMSFFQIGWPLFLQGIGLPLFFVPLTGLALSSVNEEE 363 RK+ + +LR+ N + + P LQGI +FFVPLT L LS + E Sbjct: 344 RKVATASFIGFAIVFWLRSRYNLQVDQMTVILPTLLQGIPTAMFFVPLTVLLLSGLPPER 403 Query: 364 TASAAGLMSFCRTMAGAIATSIVNTSWENDATYFHAELSGLVDRSG----DAVNAMMAS- 418 +AAGL SF R GA+ TSI T+W N + HA+L+ + VN + ++ Sbjct: 404 IPAAAGLSSFVRVFCGAVGTSISTTAWNNRSIMHHAQLTEHATPYSPWLQETVNNLSSTL 463 Query: 419 GMSAEQATGSLAQTVQNQAVMLATNHIFMMAAVSF 453 G++AEQ G L +T+ QA ML N IF ++AV F Sbjct: 464 GLNAEQTAGMLERTITFQAAMLGINDIFYVSAVIF 498 >gi|300312486|ref|YP_003776578.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 514 Score = 199 bits (506), Expect = 5e-52, Method: Compositional matrix adjust. Identities = 145/485 (29%), Positives = 245/485 (50%), Gaps = 22/485 (4%) Query: 4 NVSVSTIAGALGASTSQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFILSMVMFGLFS 63 N S+ TI G +GAS ++GT++ T+Y VAE + +P++ WL G + + + +F LFS Sbjct: 37 NSSLPTIQGEIGASGTEGTWIATAYLVAEIVIIPMSAWLERLLGLRVLLLTAASLFTLFS 96 Query: 64 ALCGLANSLGMLIAFRVMQGLAGGPLMPLSQTLLLRIFPKEKAPAAVGLWAMTTLVAPIA 123 LCGL+ +L M+I RV QG GG L+P + T++ P+ + P ++ T ++ P+ Sbjct: 97 VLCGLSTTLPMMIVGRVGQGFTGGALIPTAMTIIATRLPRSQQPVGNAMFGATAILGPVL 156 Query: 124 GPILGGVLCDQYSWPYIFWINVPVALICGYLGWRMLKRFETKTSKL-PIDRVGLILMVVW 182 GP++GG L + SW Y F+IN+PV L + K +L D G+ + + Sbjct: 157 GPVMGGWLTENVSWHYAFFINLPVGAALITLLLVAIPYERPKFEQLAEADWFGIAGLALG 216 Query: 183 VAALQLMLDEGKEKDWFASTEIVLLAVTAVVFFIAFLIWELTEHNPIVDLRVFRHRGFTA 242 + L ++L+EG+ + WF+S +I LA T+V+ FI I ++ +P++ LR+ R R F + Sbjct: 217 LGGLTVVLEEGEREQWFSSPDIRWLAATSVLGFILLAIGQVRARHPVIQLRLLRDRQFGS 276 Query: 243 SVTTICLAFGAFFGSVVLTPLWLQGYMGYTATWSGCISALTGILAVLTAPMVAKMSTTVD 302 V + A +G+ + P +L G GY + SG I L+G+ +L P + VD Sbjct: 277 VVMMAMIVGVALYGTAYVIPQFLSGIAGYNSLQSGWIVLLSGVPMILMMPFTPLLMRLVD 336 Query: 303 ARKLVCIGVTWLGFITFLRAFNNTDMSFFQIGWPL----FLQGIGLPLFFVPLTGLALSS 358 R V G L AF TD+S G ++G+G L + L A+ S Sbjct: 337 IRIAVGTGFLLLA----ASAFIETDLSPLSYGGSFVASQLMRGVGTVLAMLFLNQAAIRS 392 Query: 359 VNEEETASAAGLMSFCRTMAGAIATSIVNTSWENDATYFHAELSGLVDRSGDAVNAMMAS 418 V + A+GL S R + G++A + + ++ + H S ++ S +A + ++ S Sbjct: 393 VPPSQAGDASGLYSAARNLGGSLALAAIAV-IQDQRLWLH---SRRLEESLNANSDLVQS 448 Query: 419 GMSAEQ--------ATGSLAQTVQNQAVMLATNHIFMMAAVS-FSLAALAVWLAPKPTRV 469 M+A+ A + T+Q+QA+ + +F M V+ + L ++L P PT Sbjct: 449 SMTAQGHLLGGQDVALQLMGATIQSQALTMTYADLFWMLGVAILCVTPLVLFLRPLPTHA 508 Query: 470 ADTTS 474 A S Sbjct: 509 APVAS 513 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 119 bits (298), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 82/292 (28%), Positives = 148/292 (50%), Gaps = 16/292 (5%) Query: 25 ITSYAVAEAITVPLTGWLANRFGTVRVFILSMVMFGLFSALCGLANSLGMLIAFRVMQGL 84 +TSY V+ A+ +P++GW+A+RFG++ +F ++ +F L S LCG++++L +A R +QG+ Sbjct: 28 LTSYLVSLAVFIPISGWMADRFGSLTIFRSAICVFMLGSILCGVSSTLPQFVAARFLQGI 87 Query: 85 AGGPLMPLSQTLLLRIFPKEKAPAAVGLWAMTTLVAPIAGPILGGVLCDQYSWPYIFWIN 144 G ++P+ + ++LR K A+ + L P+ GP LGG + + W +IF+IN Sbjct: 88 GGAMMVPVGRLVILRSVEKVNLVKALSYLTIPALFGPVLGPPLGGFITTYFHWRWIFFIN 147 Query: 145 VPVALICGYLGWRMLKRFETKTSKLPIDRVGLILMVVWVAALQLMLDEGKEKDWFASTEI 204 +P++++ YL R + + + P+D G +L + + LM+ S E+ Sbjct: 148 IPISILGLYLATRFIPDLKAEQVA-PLDLKGFLLSALGCSL--LMMGLATAGRHLVSGEV 204 Query: 205 VLLAVTAVVFFIAFLIWELTEHN-PIVDLRVFRHRGFTASVTTICLAFGAFFGSV----- 258 L V A + F+A +W + P++DL + + A V FG F V Sbjct: 205 ALTCVLAGLVFLAAYVWHARRASAPLIDLALLKIPTLRAGV------FGGSFYRVGIGAI 258 Query: 259 -VLTPLWLQGYMGYTATWSGCISALTGILAVLTAPMVAKMSTTVDARKLVCI 309 L PL LQ GYT SG ++ + A+ + A+ R+++ + Sbjct: 259 PFLLPLMLQLGFGYTPFHSGMLTCASAAGAMFMKTIAARFLRRYGFRRVLLV 310 >gi|300311040|ref|YP_003775132.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 113 bits (283), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 88/383 (22%), Positives = 176/383 (45%), Gaps = 10/383 (2%) Query: 25 ITSYAVAEAITVPLTGWLANRFGTVRVFILSMVMFGLFSALCGLANSLGMLIAFRVMQGL 84 +T+Y A + +P++GW+A++ G +VF +M++F S LC ++ SL +A R +QG+ Sbjct: 28 MTAYVAALGVFIPISGWMADKLGARKVFSAAMIVFMTGSILCAVSGSLVTFVAARFLQGI 87 Query: 85 AGGPLMPLSQTLLLRIFPKEKAPAAVGLWAMTTLVAPIAGPILGGVLCDQYSWPYIFWIN 144 G ++P+ + ++ R K + AV + L+ P+ GP+LGG + + W ++F IN Sbjct: 88 GGAMMVPVGRVIIARSVDKSELVKAVSYLTLPALLGPVVGPLLGGFITTYFHWRWVFLIN 147 Query: 145 VPVALICGYLGWRMLKRFETKTSKLPIDRVGLILMVVWVAALQLMLDEGKEKDWFASTEI 204 +P + YL R ++ F+ + + P+D G +L V A + L L +T + Sbjct: 148 IPFCFLGLYLARRFIQNFK-EDNPAPLDVKGFVLTSVGAAMIMLGLSLFGSHLVDGATPV 206 Query: 205 VLLAVTAVVFFIAFLIWELTEHNPIVDLRVFRHRGFTASVTTICLAFGAFFGSV-VLTPL 263 + ++ A+ + + E P++D R+ R ASV L F G+V L PL Sbjct: 207 AMCSIGAIALVMYYRHSTQIEF-PLLDFRLLRIPTLHASVVGGSL-FRIGLGAVPFLLPL 264 Query: 264 WLQGYMGYTATWSGCISALTGILAVLTAPMVAKMSTTVDARKLVCIGVTWLGFITFLRAF 323 LQ +G TA +G I+ + ++ + ++ R ++ + + G Sbjct: 265 ALQEGLGMTAFQAGSITCASAFGSLFIRLLAGRVLRRYGFRTVLMVNAVFAGIALAAYGI 324 Query: 324 NNTDMSFFQIGWPLFLQGIGLPLFFVPLTGLALSSVNEEETASAAGLMSFCRTMAGAIAT 383 + + I + + G+ L F L +A + ++ + L S + ++ + Sbjct: 325 FSIHTPYLLIVSIVLMGGVFPALQFTCLNSIAYADIDSRDAGRVTSLASVIQQLSLGLGV 384 Query: 384 SI------VNTSWENDATYFHAE 400 ++ ++ + AT A+ Sbjct: 385 TVAGIVLQLSNHLQGHATIVEAD 407 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.136 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,421,458 Number of extensions: 58349 Number of successful extensions: 363 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 345 Number of HSP's successfully gapped: 18 Length of query: 476 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 387 Effective length of database: 1,198,455 Effective search space: 463802085 Effective search space used: 463802085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)