BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0290.p (300 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312579|ref|YP_003776671.1| hydrolase [Herbaspirillum serop... 563 e-162 gi|300313233|ref|YP_003777325.1| alpha/beta hydrolase superfamil... 94 1e-20 gi|300311290|ref|YP_003775382.1| hydrolase or acyltransferase [H... 63 4e-11 >gi|300312579|ref|YP_003776671.1| hydrolase [Herbaspirillum seropedicae SmR1] Length = 300 Score = 563 bits (1450), Expect = e-162, Method: Compositional matrix adjust. Identities = 264/299 (88%), Positives = 282/299 (94%) Query: 1 MRQTIAKKISRSEFLDVRGLRYHVRHWGDPQAPKIFMMHGWMDMSASFQFMVDALQAEWH 60 MR T+AK SRSEFL +RGLRYHVRHWGDP+APKIFM+HGWMDMSASFQFMVDALQ +WH Sbjct: 1 MRPTVAKTASRSEFLTIRGLRYHVRHWGDPEAPKIFMLHGWMDMSASFQFMVDALQGQWH 60 Query: 61 VIAPDWRGFGLTERAPAQSYWFPDYLADLDAILDHYAGEEAINLLGHSMGGNVVSLYAGV 120 VIAPDWRGFGLTER+ A SYWFPDYLADLDAI+DHYAG EAINLLGHSMGGNVV+LYAGV Sbjct: 61 VIAPDWRGFGLTERSQADSYWFPDYLADLDAIIDHYAGSEAINLLGHSMGGNVVTLYAGV 120 Query: 121 RPQRIKRLVNLEGIGLPTAKPEQAPGRFTQWLDEMKNRPVLRGYETLEEVAARLQKNNAR 180 RPQRIKRL+NLEG+GLP +PEQAPGRF QW+DE++NRPVLRGY TLEEVA RLQ+NNAR Sbjct: 121 RPQRIKRLINLEGLGLPATQPEQAPGRFAQWMDEVRNRPVLRGYATLEEVAQRLQRNNAR 180 Query: 181 LSDERAGFLAQHWAQQDESGLWQILGDPAHKQVSPILYRVDEMMACWSRITAPVLWIEAI 240 LSDERA FLAQHWAQQD+SGLWQILGDPAHKQVSPILYR DEMMACW RITAPVLWIEAI Sbjct: 181 LSDERAAFLAQHWAQQDDSGLWQILGDPAHKQVSPILYRADEMMACWRRITAPVLWIEAI 240 Query: 241 DSDIWRFFGPASLKREEIDRRIACIPKTQVEMIADAGHMLHHDQPERLAQLIEAFLQQP 299 DSDIWRFFGPASLKREEIDRRIACIP+ QVEMIADAGHMLHHDQPE+LAQLIEAFLQQP Sbjct: 241 DSDIWRFFGPASLKREEIDRRIACIPRVQVEMIADAGHMLHHDQPEQLAQLIEAFLQQP 299 >gi|300313233|ref|YP_003777325.1| alpha/beta hydrolase superfamily protein [Herbaspirillum seropedicae SmR1] Length = 289 Score = 94.0 bits (232), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 80/298 (26%), Positives = 128/298 (42%), Gaps = 32/298 (10%) Query: 11 RSEFLDVRGLRYHVRHWGDPQAPKIFMMHGWMDMSASFQFMVDALQAEWHVIAPDWRGFG 70 R L + L +H+ WGDPQ + ++HG +F + L + IA D RG G Sbjct: 4 RDYLLTLSALNFHITEWGDPQGFPLLLLHGIRGYGETFGDLAQRLPPGYRCIALDQRGRG 63 Query: 71 LTERAPAQSYWFPDYLADLDAILDHYAGEEAINLLGHSMGGNVVSLYAGVRPQRIKRLV- 129 ++ +Y+ Y+AD++A++ + +LLGHSMGG +YA P +++RL+ Sbjct: 64 QSDWDAGANYYTDAYVADVEALVARLNLRQ-FDLLGHSMGGINAIVYAARHPAKVRRLII 122 Query: 130 ---------NLEGIGLPTAKPEQAPGRFTQWLDEMKNRPVLRGYETLEEVAARLQKNNAR 180 N G + P F W + LR T E RL +N Sbjct: 123 EDAGPGAFENSPGATRIRRELATTPSHFASWDEASTFMRALRPSVTEEARQQRL--HNML 180 Query: 181 LSDERAGFLAQHWAQQDESGLWQILGDPAHKQVSPILYRVDEMMACWSRITAPVLWIEAI 240 + G+ W + D G+ + ++SP L RV ++ + P L + Sbjct: 181 KPAAQGGYT---W-RYDHVGI-------SSTRLSPDLSRVVDLRPHIMALMCPTLVVRGE 229 Query: 241 DSDIWRFFGPASLKREEIDRRIACIPKTQVEMIADAGHMLHHDQPERLAQLIEAFLQQ 298 SD A ++ +++ RI IADAGH +H DQPE A+++ FL Q Sbjct: 230 RSDYLSAEMAAEMR--QLNARIL------TSTIADAGHYIHDDQPEAFAEVVRQFLAQ 279 >gi|300311290|ref|YP_003775382.1| hydrolase or acyltransferase [Herbaspirillum seropedicae SmR1] Length = 288 Score = 62.8 bits (151), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 78/282 (27%), Positives = 120/282 (42%), Gaps = 32/282 (11%) Query: 25 RHWGDPQAPKIFM-MHGWMDMSASFQFMVDALQAEWHVIAPDWRGFGLTER-APAQSYWF 82 + WGD + P + + +HG +S F M L + VI PD G G + R A Q Y Sbjct: 23 KEWGDARNPNVLVCVHGVTRVSDDFDRMARELCDTYRVICPDVVGRGRSGRLANPQFYTV 82 Query: 83 PDYLADLDAILDHYAGEEAINLLGHSMGGNVVSLYAGVRPQRIKRLVNLEGIGLPTAKPE 142 P Y++D+ +L + ++ G SMGG + A + I+RLV L IG P+ Sbjct: 83 PQYVSDMVTLLARLDA-QIVDWFGTSMGGLIGMALASLPGNPIRRLV-LNDIG-PSIN-G 138 Query: 143 QAPGRFTQWLDEMKNRPVLRGYETLEEVAARLQKNNARL---SDERAGFLAQHWAQQDES 199 A R ++ + +++ EE A ++ +A SDE LA+ +Q+E Sbjct: 139 AALARIGDYIGQDVR------FDSFEEAAQYIRTISASFGQHSDEEWHKLAKDVLRQNEQ 192 Query: 200 GLWQILGDPA----HKQVSPILYRVDEMM--ACWSRITAPVLWIEAIDSDIWRFFGPASL 253 G W D A KQ +P E M A + I P L + SD+ Sbjct: 193 GQWVRHYDLALALPFKQTTPEAASAAEQMLWAAYDAIRCPTLLVRGAQSDLL-----LPE 247 Query: 254 KREEIDRRIACIPKTQVEMIADAGH---MLHHDQPERLAQLI 292 +E+ RR PK ++ + D GH +H Q E Q + Sbjct: 248 VAQEMTRR---GPKAKLVELPDVGHAPTFMHAPQIEVARQFL 286 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.137 0.437 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,067,069 Number of extensions: 48225 Number of successful extensions: 163 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 162 Number of HSP's successfully gapped: 4 Length of query: 300 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 215 Effective length of database: 1,217,395 Effective search space: 261739925 Effective search space used: 261739925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 130 (54.7 bits)