BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0267.p (473 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312596|ref|YP_003776688.1| 3-isopropylmalate dehydratase l... 917 0.0 gi|300312283|ref|YP_003776375.1| aconitate hydratase [Herbaspiri... 93 4e-20 gi|300311598|ref|YP_003775690.1| aconitate hydratase [Herbaspiri... 80 5e-16 >gi|300312596|ref|YP_003776688.1| 3-isopropylmalate dehydratase large subunit [Herbaspirillum seropedicae SmR1] Length = 466 Score = 917 bits (2370), Expect = 0.0, Method: Compositional matrix adjust. Identities = 443/448 (98%), Positives = 446/448 (99%) Query: 1 MLKTLYDKLWESHVVHTEDDGTAILYIDRHLLHEVTSPQAFEGLKLAGRQPWRNSANLMV 60 MLKTLYDKLWESHVVHTEDDGTAILYIDRHLLHEVTSPQAFEGLKLAGRQPWRNSANLMV Sbjct: 1 MLKTLYDKLWESHVVHTEDDGTAILYIDRHLLHEVTSPQAFEGLKLAGRQPWRNSANLMV 60 Query: 61 ADHNVPTTDRAQGIADPISRLQVETLDGNAKEFSLTYFGMNDKRQGIVHVIGPEQGATLP 120 ADHNVPTTDRA GIADPISRLQVETLDGNAKE+SLTYFGMNDKRQGIVHVIGPEQGATLP Sbjct: 61 ADHNVPTTDRAHGIADPISRLQVETLDGNAKEYSLTYFGMNDKRQGIVHVIGPEQGATLP 120 Query: 121 GMTVVCGDSHTSTHGAFAALAHGIGTSEVEHVLATQTLLARKSKAMLVQVDGALPAGVTA 180 GMTVVCGDSHTSTHGAFAALAHGIGTSEVEHVLATQTLLARKSKAMLVQVDGALPAGVTA Sbjct: 121 GMTVVCGDSHTSTHGAFAALAHGIGTSEVEHVLATQTLLARKSKAMLVQVDGALPAGVTA 180 Query: 181 KDIVLAVIGKIGTAGGTGYAIEFAGSTIRSLSMEGRMTVCNMAIEAGARAGMVAFDDTTL 240 KDIVLAVIGKIGTAGGTGYAIEFAGSTIRSLSMEGRMTVCNMAIEAGARAGMVAFDDTTL Sbjct: 181 KDIVLAVIGKIGTAGGTGYAIEFAGSTIRSLSMEGRMTVCNMAIEAGARAGMVAFDDTTL 240 Query: 241 EYLKGRPFSPSGPHWERAVSYWRTLHSDQGAKFDLVVTLNAAEIKPQVTWGTSPEMVVAV 300 EYLKGRPFSPSGPHWERAVSYWR+LHSD GAKFD+VVTLNAAEIKPQVTWGTSPEMVVAV Sbjct: 241 EYLKGRPFSPSGPHWERAVSYWRSLHSDPGAKFDMVVTLNAAEIKPQVTWGTSPEMVVAV 300 Query: 301 DARVPDPDKEKDPTKRDGMEKALAYMALKPNTPIEDIRIDKVFIGSCTNSRIEDLREAAK 360 DARVPDPDKEKDPTKRDGMEKALAYMALKPNTPIEDIRIDKVFIGSCTNSRIEDLREAAK Sbjct: 301 DARVPDPDKEKDPTKRDGMEKALAYMALKPNTPIEDIRIDKVFIGSCTNSRIEDLREAAK 360 Query: 361 VVRGKFRASNVKLAMVVPGSGLVKEQAEREGLDKIFRDAGFEWREPGCSMCLAMNADRLE 420 VVRGKFRASNVKLAMVVPGSGLVKEQAEREGLDKIFRDAGFEWREPGCSMCLAMNADRLE Sbjct: 361 VVRGKFRASNVKLAMVVPGSGLVKEQAEREGLDKIFRDAGFEWREPGCSMCLAMNADRLE 420 Query: 421 PGERCASTSNRNFEGRQGAGGRTHLVSP 448 PGERCASTSNRNFEGRQGAGGRTHLVSP Sbjct: 421 PGERCASTSNRNFEGRQGAGGRTHLVSP 448 >gi|300312283|ref|YP_003776375.1| aconitate hydratase [Herbaspirillum seropedicae SmR1] Length = 903 Score = 93.2 bits (230), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 110/388 (28%), Positives = 160/388 (41%), Gaps = 36/388 (9%) Query: 98 FGMNDKRQGIVHVIGPEQ---------GATLPGMTVVCGDSHTSTHGAFAALAHGIGTSE 148 FG+ GIVH + E G P T+V DSHT+ + G+G E Sbjct: 171 FGVVPPGFGIVHQVNLEYLARGVHNKTGVYYPD-TLVGTDSHTTMINGIGVVGWGVGGIE 229 Query: 149 VEHVLATQTLLARKSKAMLVQVDGALPAGVTAKDIVLAVIGKIGTAGGTGYAIEFAGSTI 208 E + Q + + V + GAL GVTA D+VL + + G +EF G Sbjct: 230 AEAGMLGQPVYFLTPDVVGVNLTGALREGVTATDLVLTITELLRQTKVVGKFVEFFGEGT 289 Query: 209 RSLSMEGRMTVCNMAIEAGARAGMVAFDDTTLEYLKGRPFSPSGPHWERAVSYWRTLHS- 267 SLS+ R T+ NMA E GA G DD T+EY KG + + A + L+ Sbjct: 290 ESLSLTDRATIANMAPEYGATMGFFPVDDATIEYFKGTGRTKAEIDAFEAYFKAQELYGV 349 Query: 268 DQGAKFDLV--VTLNAAEIKPQVTWGTSPEMVVAV-DARVPDPDKEKDPTKRDGM-EKAL 323 + + D V+L+ + P + P+ + + + + D PT +G +KA Sbjct: 350 PKAGQIDYTQEVSLDLGTVAPSLAGPKRPQDRIEIGNVKSTFSDLFTKPTAENGFNKKAE 409 Query: 324 AYMALKPNTPIEDIRIDKVFIG---SCTNSRIEDLREAAKVVRGKFRASNVKLA-----M 375 A N D+ V I SCTN+ + AA ++ K + +K+A Sbjct: 410 DLGASYKNADGVDLHNGDVLIAAITSCTNTSNPSVLLAAGLLAKKAVEAGLKVAPHIKTS 469 Query: 376 VVPGSGLVKEQAEREGLDKIFRDAGFEWREPGCSMCLAMNADRLEPGERCAS-------- 427 + PGS +V + E GL GF GC+ C+ NA L P A Sbjct: 470 LAPGSRVVTKYLEAAGLLPYLEKLGFGVTAYGCTTCIG-NAGDLTPAMNEAIVKNDVVAA 528 Query: 428 ---TSNRNFEGRQGAGGRTH-LVSPPLL 451 + NRNFE R R + L SPPL+ Sbjct: 529 AVLSGNRNFEARIHPNIRANFLASPPLV 556 >gi|300311598|ref|YP_003775690.1| aconitate hydratase [Herbaspirillum seropedicae SmR1] Length = 873 Score = 79.7 bits (195), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 105/377 (27%), Positives = 147/377 (38%), Gaps = 66/377 (17%) Query: 110 VIGPEQGATLPGMTVVCGDSHTSTHGAFAALAHGIGTSEVEHVLATQTLLARKSKAMLVQ 169 VI + G P T+V DSHT A +A G+G E E+V+ + R + V+ Sbjct: 183 VIHAKDGVAFPD-TLVGTDSHTPHVDALGVIAIGVGGLEAENVMLGRASWMRLPDIIGVE 241 Query: 170 VDGALPAGVTAKDIVLAVIGKIGTAGGTGYAIEFAGSTIRSLSMEGRMTVCNMAIEAGAR 229 + G G+TA DIVL++ + G +EF G SL++ R T+ NMA E GA Sbjct: 242 LTGRRQPGITATDIVLSLTEFLRKQKVVGAYLEFYGDGAASLTLGDRATISNMAPEYGAT 301 Query: 230 AGMVAFDDTTLEYLK--GRP---------FSPSGPHWERA---VSYWRTLHSDQGAKFDL 275 A M + D T++YLK GR ++ W V Y R L +FDL Sbjct: 302 AAMFSIDQQTIDYLKLTGREDEQVKLVETYAKEAGLWSDTLAKVEYERVL------RFDL 355 Query: 276 --VVTLNAAEIKPQVTWGTSPEMVVAVDARVPDPDKEKDPTKRDGMEKALAYMALKPNTP 333 VV A P S + +V E +P K Sbjct: 356 STVVRTLAGPSNPHKRLPVSELAAQGIAGKV-----ENEPGK------------------ 392 Query: 334 IEDIRIDKVFIGSCTN-SRIEDLREAAKVVR-----GKFRASNVKLAMVVPGSGLVKEQA 387 + D + I SCTN S ++ A + R G R VK ++ PGS V+ Sbjct: 393 MPDGAVIIAAITSCTNTSNPRNVIAAGLLARNANRLGLARKPWVKSSL-APGSKAVELYL 451 Query: 388 EREGLDKIFRDAGFEWREPGCSMCLAMNADRLEPG-----------ERCASTSNRNFEGR 436 E GL GF C+ C M+ L+P + NRNF+GR Sbjct: 452 EEAGLTADLEKLGFGIVAFACTTCNGMSG-ALDPKIQQEIIDRDLYSTAVLSGNRNFDGR 510 Query: 437 -QGAGGRTHLVSPPLLT 452 + L SPPL+ Sbjct: 511 IHPYAKQAFLASPPLVV 527 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,481,744 Number of extensions: 61118 Number of successful extensions: 197 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 196 Number of HSP's successfully gapped: 3 Length of query: 473 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 384 Effective length of database: 1,198,455 Effective search space: 460206720 Effective search space used: 460206720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)