BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0015.0256.p
         (242 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protei...   223   9e-60
gi|300310189|ref|YP_003774281.1| transmembrane protein [Herbaspi...    58   6e-10

>gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protein
           [Herbaspirillum seropedicae SmR1]
          Length = 695

 Score =  223 bits (569), Expect = 9e-60,   Method: Compositional matrix adjust.
 Identities = 122/145 (84%), Positives = 127/145 (87%)

Query: 27  MLIANYDKPLVVVSLLVAILASYTALDMAERINTTSHLHSRAARFWLAGGAVAMGLGIWS 86
           MLIA YDK LVVVSLLVAILASYTALDMAERINTT+  H RAAR+WLAGGAVAMGLGIWS
Sbjct: 1   MLIATYDKLLVVVSLLVAILASYTALDMAERINTTASQHGRAARWWLAGGAVAMGLGIWS 60

Query: 87  MHFIGMLAFRLPIALGYDVWITTLSLLIAIATSGYALWIVTRSELPLRRLFGSALLMGTG 146
           MHFIGMLAFRLPIALGYD  IT LSL+IAIA SG+ALWIVTR ELP RRL GSAL MG G
Sbjct: 61  MHFIGMLAFRLPIALGYDAGITALSLVIAIAVSGFALWIVTRPELPTRRLLGSALFMGIG 120

Query: 147 IAGMHYTGMAAMRMTPGIDYDLVLF 171
           IA MHYTGMAA+RM PGI YDL LF
Sbjct: 121 IACMHYTGMAALRMQPGIAYDLFLF 145


>gi|300310189|ref|YP_003774281.1| transmembrane protein
           [Herbaspirillum seropedicae SmR1]
          Length = 706

 Score = 58.2 bits (139), Expect = 6e-10,   Method: Compositional matrix adjust.
 Identities = 44/135 (32%), Positives = 77/135 (57%), Gaps = 5/135 (3%)

Query: 36  LVVVSLLVAILASYTALDMAERINTTSHLHSRAARFWLAGGAVAMGLGIWSMHFIGMLAF 95
           L+  ++LVA L++Y AL+++ER+      H R    WL+ GAV +GLG+W+  F+ + A 
Sbjct: 9   LLAFAVLVASLSAYAALEVSERMVMQESQHRR---LWLSVGAVVLGLGVWATLFLTVAAL 65

Query: 96  RLPIALGYDVWITTLSLLIAIATSGYALWIV-TRSELPLRRLFGSALLMGTGIAGMHYTG 154
            L + +G+D+ +T L+LL+ + ++ Y + +   R   P R   G  L +G  I    +  
Sbjct: 66  GLSMPVGFDIRLTVLALLLCLGSAFYLMHLSGLRRPHPSRLAIG-GLSIGAAINAAFHMA 124

Query: 155 MAAMRMTPGIDYDLV 169
           +AAM++ P + Y  V
Sbjct: 125 LAAMQLLPALLYQQV 139


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.326    0.138    0.416 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 664,257
Number of extensions: 23875
Number of successful extensions: 113
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 108
Number of HSP's successfully gapped: 3
Length of query: 242
Length of database: 1,619,870
Length adjustment: 83
Effective length of query: 159
Effective length of database: 1,226,865
Effective search space: 195071535
Effective search space used: 195071535
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 129 (54.3 bits)