BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0245.p (360 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312610|ref|YP_003776702.1| spermidine/putrescine ABC trans... 572 e-164 gi|300311547|ref|YP_003775639.1| ABC transporter ATPase [Herbasp... 223 2e-59 gi|300313814|ref|YP_003777906.1| spermidine/putrescine ABC trans... 211 5e-56 gi|300310986|ref|YP_003775078.1| sulfate/molybdate ABC transport... 205 5e-54 gi|300309936|ref|YP_003774028.1| spermidine/putrescine ABC trans... 200 1e-52 >gi|300312610|ref|YP_003776702.1| spermidine/putrescine ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 434 Score = 572 bits (1475), Expect = e-164, Method: Compositional matrix adjust. Identities = 298/374 (79%), Positives = 320/374 (85%), Gaps = 20/374 (5%) Query: 1 MFLEVDHVSIRYPRSAQAAVDAVSLRLQPGELGALIGPSGSGKTSLLRAVAGLEPAQSGR 60 MFLEV+H+SIRYPR+ AVDAVSLRL PGELGALIGPSGSGKTSLLRAVAGLEPAQ+G Sbjct: 22 MFLEVEHLSIRYPRTPAPAVDAVSLRLAPGELGALIGPSGSGKTSLLRAVAGLEPAQAGL 81 Query: 61 IRLDGALLEGDGVRVPAEARRIGMVFQDFALFPHLDVAANIGFGLRGMARAQREQIVTQM 120 IRLDG LLEG GVRVPAE RRIGMVFQDFALFPHL+VA NI FGLRG + +R IV M Sbjct: 82 IRLDGQLLEGGGVRVPAEQRRIGMVFQDFALFPHLNVADNIAFGLRGRDKRERSAIVAGM 141 Query: 121 LALVGLPEAGSKFPHQLSGGQQQRIALARALAPQPRLLLLDEPFSSLDVELRERLAHDVR 180 LALVGLPEAG+KFPHQLSGGQQQRIALARALAPQPRLLLLDEPFSSLDVELRERLAHDVR Sbjct: 142 LALVGLPEAGAKFPHQLSGGQQQRIALARALAPQPRLLLLDEPFSSLDVELRERLAHDVR 201 Query: 181 DILKRTGTTALMVTHDQLEAFAVADVVGVMRHGRLEQWDTPYALYHRPATRFAADFIGHG 240 DILKRT TTALMVTHDQ+EAFAVADVVGVMR G+LEQWDTPYALYHRPATRFAADFIGHG Sbjct: 202 DILKRTHTTALMVTHDQMEAFAVADVVGVMRQGQLEQWDTPYALYHRPATRFAADFIGHG 261 Query: 241 VLLHGEVQAAADAGVKG----------------EISVQTAAGLLRGLPVESGRDASAAQ- 283 VLL G+++ DA + EI V+TAAGLLRG+P+ + AAQ Sbjct: 262 VLLQGQLR-GPDANAQALASRPATDHHQGDEQEEIEVETAAGLLRGVPLHALHGQQAAQT 320 Query: 284 --VDVLLRADDVMHDDASPFKAQVVRKSFRGAEFLYTLALENGQQLLSLVPSHHDHAVGE 341 VDVLLRADDV+HDDASPF+A+VVRKSFRGAEFLYTLALENGQ LLSL PSHHDHAVGE Sbjct: 321 SEVDVLLRADDVIHDDASPFRARVVRKSFRGAEFLYTLALENGQTLLSLAPSHHDHAVGE 380 Query: 342 WIGIRLDVTHLVTF 355 WIGIRL+VTHLVTF Sbjct: 381 WIGIRLEVTHLVTF 394 >gi|300311547|ref|YP_003775639.1| ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 223 bits (568), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 153/357 (42%), Positives = 214/357 (59%), Gaps = 29/357 (8%) Query: 1 MFLEVDHVSIRYPRSAQAAVDAVSLRLQPGELGALIGPSGSGKTSLLRAVAGLEPAQSGR 60 +FL V V R+ + A+D VSL ++ GE+ L+GPSG GKT+LLR +AGLE SGR Sbjct: 11 VFLSVREVEKRF--GSFTALDRVSLEVRRGEMVCLLGPSGCGKTTLLRTIAGLERQDSGR 68 Query: 61 IRLDGALLEGDGVRVPAEARRIGMVFQDFALFPHLDVAANIGFGLRGMARAQREQIVTQM 120 + DG D ++P +AR G++FQ +ALFP+L VA N+ +GL M+R Q+ + VT+M Sbjct: 69 LHADGR----DISQLPPQARDYGILFQSYALFPNLSVADNVAYGLGRMSRQQKRERVTEM 124 Query: 121 LALVGLPEAGSKFPHQLSGGQQQRIALARALAPQPRLLLLDEPFSSLDVELRERLAHDVR 180 L++VGL + K+P QLSGGQQQR+ALARALAP P LLLLDEP S+LD ++RE L ++R Sbjct: 125 LSMVGLDGSQDKYPGQLSGGQQQRVALARALAPSPSLLLLDEPLSALDAQVREHLQLEIR 184 Query: 181 DILKRTGTTALMVTHDQLEAFAVADVVGVMRHGRLEQWDTPYALYHRPATRFAADFIGHG 240 + K+ T LMVTHDQ EA +AD + VM+HGR+EQ+ TP +Y RPAT F ADFIG Sbjct: 185 RLQKQFRITTLMVTHDQEEAMVMADRIAVMQHGRIEQFGTPEQIYRRPATPFVADFIGQA 244 Query: 241 VLLHGEVQAAADAGVKG-EISVQTAAGLLRGLPVESGRDASAAQVDVLLRADDVMHDD-- 297 L + + V G + +Q P ES D ++ ++ A + D+ Sbjct: 245 NWLPFTRLSGSQVAVGGLHLEIQ---------PDES--DRASGRLFCRPEAVQLFSDESC 293 Query: 298 ASPFKAQVVRKSFRGAEFLYTLALEN--GQQLLSLVPSHHDHAV-------GEWIGI 345 A+ F A+VV + + G + LA + GQ LL+ V S V G W+ + Sbjct: 294 ANRFLARVVDRIYLGDRYRLALAADALPGQTLLADVSSQAHERVASLDTQDGLWVTL 350 >gi|300313814|ref|YP_003777906.1| spermidine/putrescine ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 362 Score = 211 bits (538), Expect = 5e-56, Method: Compositional matrix adjust. Identities = 124/276 (44%), Positives = 178/276 (64%), Gaps = 10/276 (3%) Query: 3 LEVDHVSIRYPRSAQAA--VDAVSLRLQPGELGALIGPSGSGKTSLLRAVAGLEPAQSGR 60 L V+ + + Y A A + VS+ LQ GE+ AL+GPSGSGKT+LLRAVAGLE ++G Sbjct: 4 LSVNDLHLDYGTGAHANPILKGVSMELQRGEVVALLGPSGSGKTTLLRAVAGLESPKAGA 63 Query: 61 IRLDGALLEGDGVR---VPAEARRIGMVFQDFALFPHLDVAANIGFGLRGMARAQREQIV 117 I + G DG R +PAE R +G+VFQ +AL+PH VA N+ +GL+ + + +I Sbjct: 64 IDI-GKRRVFDGARNFEMPAEERNLGLVFQSYALWPHKTVADNVAYGLK-LRKTPSGEIA 121 Query: 118 TQM---LALVGLPEAGSKFPHQLSGGQQQRIALARALAPQPRLLLLDEPFSSLDVELRER 174 T++ L+ +GL G ++PHQLSGGQQQR+A+ARAL P+++LLDEP S+LD +LRE Sbjct: 122 TRVKAVLSQLGLGHLGERYPHQLSGGQQQRVAIARALVYNPQVILLDEPLSNLDAKLREE 181 Query: 175 LAHDVRDILKRTGTTALMVTHDQLEAFAVADVVGVMRHGRLEQWDTPYALYHRPATRFAA 234 +R+++ R G +ALMVTHDQ EA A++D + ++ +GR+EQ TP ++Y P T F A Sbjct: 182 ARAFLRELIVRLGLSALMVTHDQGEAMAISDRILLLNNGRIEQQGTPQSMYQAPNTLFTA 241 Query: 235 DFIGHGVLLHGEVQAAADAGVKGEISVQTAAGLLRG 270 +F+G L EV + V ++ TA LRG Sbjct: 242 EFMGSNNKLAAEVLSREGERVTVKLDGGTAVATLRG 277 >gi|300310986|ref|YP_003775078.1| sulfate/molybdate ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 205 bits (521), Expect = 5e-54, Method: Compositional matrix adjust. Identities = 125/279 (44%), Positives = 171/279 (61%), Gaps = 18/279 (6%) Query: 1 MFLEVDHVSIRYPRSAQAAVDAVSLRLQPGELGALIGPSGSGKTSLLRAVAGLEPAQSGR 60 M +EV H+ R+ + A++ VSL GEL AL+GPSG GKT+LLR +AGLE SG+ Sbjct: 1 MSIEVHHIQKRF--GSFTALNDVSLNFPAGELTALLGPSGCGKTTLLRIIAGLESPDSGQ 58 Query: 61 IRLDGALLEGDGVRVPAEARRIGMVFQDFALFPHLDVAANIGFGLRGMARAQR------E 114 + LDG VR R++G VFQ +ALF H+ V NI FGLR R+QR Sbjct: 59 VLLDGEDASARHVR----ERQVGFVFQHYALFKHMTVFENIAFGLRVKPRSQRPSEAVIR 114 Query: 115 QIVTQMLALVGLPEAGSKFPHQLSGGQQQRIALARALAPQPRLLLLDEPFSSLDVELRER 174 + V ++L LV L ++P QLSGGQ+QRIALARALA +PR+LLLDEPF +LD ++R+ Sbjct: 115 EKVKRLLELVQLDWLADRYPPQLSGGQRQRIALARALAVEPRVLLLDEPFGALDAKVRKE 174 Query: 175 LAHDVRDILKRTGTTALMVTHDQLEAFAVADVVGVMRHGRLEQWDTPYALYHRPATRFAA 234 L +R + T++ VTHDQ EA VAD V +M G++EQ TP A+Y+ PA+ F Sbjct: 175 LRRWLRRLHDELHVTSIFVTHDQEEALEVADQVVLMNKGQVEQIGTPEAVYNHPASPFVY 234 Query: 235 DFIGHGVLLHGEV-QAAADAG-----VKGEISVQTAAGL 267 F+G+ L HG + + DAG G +++ AAG+ Sbjct: 235 GFLGNVNLFHGRLHEGVLDAGGARFEAPGHDNIRDAAGV 273 >gi|300309936|ref|YP_003774028.1| spermidine/putrescine ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 361 Score = 200 bits (508), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 138/371 (37%), Positives = 201/371 (54%), Gaps = 34/371 (9%) Query: 6 DHVSIRYPRSAQA------AVDAVSLRLQPGELGALIGPSGSGKTSLLRAVAGLE-PAQS 58 + VSIR + A++ A+ + L++ PGE L+GPSG GKT+ LR +AGLE P + Sbjct: 6 EAVSIRLSQCAKSFANGTRALQPLDLQIHPGETLVLLGPSGCGKTTTLRMIAGLEFPDEG 65 Query: 59 GRIRLDGALLEGDGVRVPAEARRIGMVFQDFALFPHLDVAANIGFGL--RGMARAQREQI 116 GR+ E D +P E R +GMVFQ++ALFP++ V NI +G+ R +A +R + Sbjct: 66 GRVLFG----EEDVTMLPIEKRGVGMVFQNYALFPNMSVGENIAYGMKIRKIAAGERAER 121 Query: 117 VTQMLALVGLPEAGSKFPHQLSGGQQQRIALARALAPQPRLLLLDEPFSSLDVELRERLA 176 V ++L +V L + QLSGGQ+QR+ALARALA +PR+LLLDEP ++LD +LRE + Sbjct: 122 VERLLEMVHLQGLSHRRVDQLSGGQKQRVALARALAMEPRVLLLDEPLTALDAKLREAVR 181 Query: 177 HDVRDILKRTGTTALMVTHDQLEAFAVADVVGVMRHGRLEQWDTPYALYHRPATRFAADF 236 D+ +L+ G TA+ VTHDQ EA A+ D + VM G++ Q TP +Y+ PA F A+F Sbjct: 182 SDLNKLLRSLGITAIYVTHDQGEAMALGDRIVVMERGKISQIGTPQEIYYHPANDFVAEF 241 Query: 237 IGHGVLLHGEVQAAADAGVKGEISVQTAAGLLRGLPVESGRDASAAQ-VDVLLRADDV-- 293 IG D V G+ +Q G L P DA A Q L R +DV Sbjct: 242 IG--------AMNRVDGIVAGD-RLQLPTGTLPLPP-----DAVAGQPAQALFRPEDVEV 287 Query: 294 ---MHDDASPFKAQVVRKSFRGAEFLYTLALENGQQLLSLVPSHHDHAVGEWIGIRLDVT 350 DA + +++ F G + + + + +++ G+ I IR+ T Sbjct: 288 IDITEADAHCLRGKLINTFFLGDRTRFEIEIGAERPVIAETSRRGWWEAGQQIAIRVPAT 347 Query: 351 HLVTF-PLAAN 360 LVT P AA Sbjct: 348 ALVTMAPRAAK 358 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,145,752 Number of extensions: 50212 Number of successful extensions: 635 Number of sequences better than 1.0e-08: 131 Number of HSP's gapped: 417 Number of HSP's successfully gapped: 147 Length of query: 360 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 274 Effective length of database: 1,212,660 Effective search space: 332268840 Effective search space used: 332268840 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)