BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0233.p (319 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310534|ref|YP_003774626.1| transmembrane sensor histidine ... 89 5e-19 gi|300311805|ref|YP_003775897.1| two component hybrid sensor his... 85 8e-18 gi|300312076|ref|YP_003776168.1| signal transduction histidine k... 57 1e-09 gi|300311282|ref|YP_003775374.1| transmembrane sensor histidine ... 56 4e-09 >gi|300310534|ref|YP_003774626.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 677 Score = 89.0 bits (219), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 75/253 (29%), Positives = 127/253 (50%), Gaps = 20/253 (7%) Query: 31 DSTGEGIYGIDLEGRCTFINRAGAAMLGYAPAEVLGHNMHELVHHSHEHGGHYPEAECPI 90 +ST + I+ + +G F N A LG E + + EL PE I Sbjct: 326 ESTSDPIFVKNRQGLMIFANPATLHKLGKTWEETMYRSSRELFL--------VPEEADTI 377 Query: 91 LKAFRK----GESCRVDQEVFWRADGSCFATEYSSYQIIDGGQVRGAVIAFSDISKRRQA 146 + R+ G+ +++Q + F T + DG V+G + +DI++R+QA Sbjct: 378 DRDDRRVMASGKPEKLEQTLHLPEGVVTFQTAKVPWFGKDG-SVQGVIGISTDITERKQA 436 Query: 147 EEQLQRAKAELEVRVAERTRELSAALRQVRQLSAHAHSVREDERTRIAREIHDELGSLLV 206 E++L++ +++LE V +RT L R+L+ H +VRE+E+ IARE+HD +G+ L Sbjct: 437 EDELRQRESQLEKTVIQRTALL-------RELTNHLETVREEEKRAIARELHDNMGASLT 489 Query: 207 ALKLDVNWMAKRVADLDPVARKCEAMSRLIETAVDNVGRIITDLRPSILDHQGLIAALEW 266 AL + + + + + + A + M L+++ V RI T+LRP+ LD GL AA+ Sbjct: 490 ALSMHLEGVYQILPPDEKWADRKVRMQGLMKSLVATTRRIQTELRPNTLDLFGLKAAISE 549 Query: 267 QVQEFSEATELPC 279 Q+ E E T + C Sbjct: 550 QLDELHERTGIAC 562 >gi|300311805|ref|YP_003775897.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1278 Score = 85.1 bits (209), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 46/130 (35%), Positives = 72/130 (55%), Gaps = 3/130 (2%) Query: 29 LLDSTGEGIYGIDLEGRCTFINRAGAAMLGYAP-AEVLGHNMHELVHHSHEHGGHYPEAE 87 LLDST +G + ID G T N A +LGYA +VLG +H+++ E Sbjct: 446 LLDSTDQGFFSIDSAGIITLCNAAFLQLLGYAAEKDVLGRPIHDVILQPSEE--TQSRTG 503 Query: 88 CPILKAFRKGESCRVDQEVFWRADGSCFATEYSSYQIIDGGQVRGAVIAFSDISKRRQAE 147 C +L+A R+G S VD E F R DG+ F +Y ++ + GQ++GA+ F D+++RR+ Sbjct: 504 CAVLRAAREGVSAHVDSENFLRVDGTSFPVQYQTHPVWQDGQLQGALCTFVDLTERRRGL 563 Query: 148 EQLQRAKAEL 157 + L ++ L Sbjct: 564 QALSWSENRL 573 Score = 62.4 bits (150), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 49/145 (33%), Positives = 74/145 (51%), Gaps = 10/145 (6%) Query: 40 IDLEGRCTFINRAGAAMLGYAPAEVLGHNMHELVHHSHEHGGHYPEAECPILKAFRKGES 99 +DL+GR +N+A MLG + E+LG + ++ H P + G S Sbjct: 587 LDLQGRYLRVNQALCRMLGRSEEEMLGMTLADVTHPDD-----VPMNMSLFNRCIEDGRS 641 Query: 100 CRVDQEVFWRADGSCFATEYSSYQIIDGGQVRGAVIAFS-DISKRRQAEEQLQRAKAELE 158 +++ + R DGS F +I+DG A+IA S DI++RR+AEE L+ LE Sbjct: 642 FTLEKR-YLRPDGSTFWVSSDISRIVDGEGRPQAMIAVSTDITERRRAEEALRELNDTLE 700 Query: 159 VRVAERTRELS---AALRQVRQLSA 180 RV E E S AALRQ++++ A Sbjct: 701 QRVVEEILERSRAEAALRQMQKMEA 725 >gi|300312076|ref|YP_003776168.1| signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 356 Score = 57.4 bits (137), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 35/106 (33%), Positives = 64/106 (60%), Gaps = 5/106 (4%) Query: 180 AHAHSVREDERTRIAREIHDELGSLLVALKLDVNWMAKRVADLD---PVARKCEAMSRLI 236 A A +RE ER RIAR++HD+LG +L + + + + +++A P+A EA +L+ Sbjct: 145 ATAIHIREQERERIARDLHDDLGGVLAGVGMALGRLQEQLAAQGLAAPLAEADEA-RQLL 203 Query: 237 ETAVDNVGRIITDLRPSILDHQGLIAALEWQVQEFSEATELPCEVE 282 + A+ ++ II +L P I++ GL+ AL+WQ ++ L C+++ Sbjct: 204 QQAMASMHSIIDELHPPIVEF-GLVDALQWQCRQIQREYRLHCQLD 248 >gi|300311282|ref|YP_003775374.1| transmembrane sensor histidine kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 464 Score = 55.8 bits (133), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 42/136 (30%), Positives = 66/136 (48%), Gaps = 8/136 (5%) Query: 174 QVRQLSAHAHSVREDERTRIAREIHDELGSLLVALKLDVNWMAKRVADLDPVARKC---- 229 ++RQL+ +E+ER R++R++HD + LV++KL + R+ A Sbjct: 234 KLRQLAQRVVRSQEEERARLSRDLHDGISQWLVSIKLQIEAALVRLNGTPEQAATARAGF 293 Query: 230 EAMSRLIETAVDNVGRIITDLRPSILDHQGLIAALEWQVQEFSEATELPCEVEFDIAPE- 288 E + I + V RI DLRP+ILD GL AALE E+ +A E+ F+ + Sbjct: 294 EKTATQINGVLAEVRRISHDLRPAILDDLGLAAALEHLCGEWRDAAGAGVEIVFENQAQA 353 Query: 289 ---AFVPDGTKATPFK 301 A +PD F+ Sbjct: 354 DDGATLPDVVNTVLFR 369 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 884,357 Number of extensions: 34757 Number of successful extensions: 181 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 174 Number of HSP's successfully gapped: 6 Length of query: 319 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 234 Effective length of database: 1,217,395 Effective search space: 284870430 Effective search space used: 284870430 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)