BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0228.p (414 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312616|ref|YP_003776708.1| major facilitator superfamily p... 803 0.0 gi|300313879|ref|YP_003777971.1| major facilitator superfamily p... 419 e-118 gi|300310615|ref|YP_003774707.1| major facilitator superfamily p... 93 3e-20 gi|300310449|ref|YP_003774541.1| 4-hydroxybenzoate transporter t... 91 2e-19 gi|300310666|ref|YP_003774758.1| major facilitator superfamily p... 86 7e-18 >gi|300312616|ref|YP_003776708.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 414 Score = 803 bits (2073), Expect = 0.0, Method: Compositional matrix adjust. Identities = 404/413 (97%), Positives = 408/413 (98%) Query: 1 MWLQETTTSERRTLTATFTGYAVDGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTS 60 MWLQETTTSERRTLTATFTGYAVDGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTS Sbjct: 1 MWLQETTTSERRTLTATFTGYAVDGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTS 60 Query: 61 AIGGWAAGVLADRYGRVRVLQWTVLWFALFTFLSGFTTSFGELFFTRAMQGFGMGGEWAV 120 AIGGWAAGVLADRYGRVRVLQWTVLWFA FTFLSGFTTSFG+LFFTRAMQGFGMGGEWAV Sbjct: 61 AIGGWAAGVLADRYGRVRVLQWTVLWFAFFTFLSGFTTSFGQLFFTRAMQGFGMGGEWAV 120 Query: 121 GSVLIAETISARHRGKASGLVQSSWAVGWAASALAFWGVYAVLPPETAWKVLFWIGVLPA 180 GSVLIAETISARHRGKASGLVQSSWAVGWA SALAFWGVYAVLPPETAWKVLFWIGVLPA Sbjct: 121 GSVLIAETISARHRGKASGLVQSSWAVGWAGSALAFWGVYAVLPPETAWKVLFWIGVLPA 180 Query: 181 LLTLYIRRAISEPEVFLATQARLRESGQRGNFLRIFHRDLLRTTLLASLLATGMQGAYYS 240 LLTLYIRRAISEP V+LATQARLRESGQRGNFLRIFHRDLLRTTLLASLLATGMQGAYYS Sbjct: 181 LLTLYIRRAISEPAVYLATQARLRESGQRGNFLRIFHRDLLRTTLLASLLATGMQGAYYS 240 Query: 241 VTTWLPTFLKTERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTIGRRRCFMLFAAMGIV 300 VTTWLPTFLKTERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTIGRRRCFMLFAAMGIV Sbjct: 241 VTTWLPTFLKTERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTIGRRRCFMLFAAMGIV 300 Query: 301 LVLSYTQLIITDAWMLALGFVLGFFLSGIFSGMGAYLSELFPSDVRGSGQGFCYNFGRAV 360 LVLSYTQ+IITDAWMLALGFVLGFFLSGIFSGMGAYLSELFPSDVRGSGQGFCYNFGRAV Sbjct: 301 LVLSYTQMIITDAWMLALGFVLGFFLSGIFSGMGAYLSELFPSDVRGSGQGFCYNFGRAV 360 Query: 361 GAICPALVGYLSNSLPLGQTIGWIAAGCYGLVIVACLILPETRGKELEAVVAA 413 GAICPALVGYLSNSLPLGQTIGWIAAGCYGLVIVACL+LPETRGKELEAV A Sbjct: 361 GAICPALVGYLSNSLPLGQTIGWIAAGCYGLVIVACLVLPETRGKELEAVAKA 413 >gi|300313879|ref|YP_003777971.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 413 Score = 419 bits (1077), Expect = e-118, Method: Compositional matrix adjust. Identities = 214/407 (52%), Positives = 283/407 (69%) Query: 2 WLQETTTSERRTLTATFTGYAVDGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTSA 61 W ++ T ER+T A F+G+A+D D +++ IP L+AA+S+SKA+AG I+ L+ SA Sbjct: 4 WYRQVTAKERKTFWACFSGWALDALDVQMFSLAIPALIAAFSLSKADAGLISGATLVASA 63 Query: 62 IGGWAAGVLADRYGRVRVLQWTVLWFALFTFLSGFTTSFGELFFTRAMQGFGMGGEWAVG 121 IGGW AG L+DRYGRV+ LQ T++WF+ FTFLS F SF +L +A+QGFG GGEWA G Sbjct: 64 IGGWFAGALSDRYGRVKTLQITIIWFSFFTFLSAFAQSFPQLLVLKALQGFGFGGEWAAG 123 Query: 122 SVLIAETISARHRGKASGLVQSSWAVGWAASALAFWGVYAVLPPETAWKVLFWIGVLPAL 181 +VL+AETI A HRGKA G VQS+WAVGW + L F ++++LP E AW+V+F IG+LPAL Sbjct: 124 AVLMAETIRAEHRGKAMGTVQSAWAVGWGGAVLLFSVLFSLLPQEMAWRVMFVIGLLPAL 183 Query: 182 LTLYIRRAISEPEVFLATQARLRESGQRGNFLRIFHRDLLRTTLLASLLATGMQGAYYSV 241 L +Y+RR++ E + FLA Q L IF D+LR T++ SLL G G YY++ Sbjct: 184 LVVYVRRSVQETDAFLAGQKAAASKPAASTLLGIFRPDVLRLTIIGSLLGVGAHGGYYAL 243 Query: 242 TTWLPTFLKTERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTIGRRRCFMLFAAMGIVL 301 TWLPT+LKTE+HLSVL T YL V+I +CG + S+ L D IGRRR FA + Sbjct: 244 MTWLPTYLKTEKHLSVLSTGGYLAVIIVAFWCGCIASSQLLDRIGRRRTVAFFALACVAT 303 Query: 302 VLSYTQLIITDAWMLALGFVLGFFLSGIFSGMGAYLSELFPSDVRGSGQGFCYNFGRAVG 361 VL Y L ++D ML GF LGFF +GI + MGA +EL+PS +RG+G GFCYNFGR Sbjct: 304 VLCYIFLPLSDTQMLVCGFPLGFFAAGIPASMGALFNELYPSGIRGTGVGFCYNFGRVAS 363 Query: 362 AICPALVGYLSNSLPLGQTIGWIAAGCYGLVIVACLILPETRGKELE 408 A P LVG++S+S+PLGQ IG AA Y LV+VA L+LPET+G+++E Sbjct: 364 AGFPVLVGHMSDSMPLGQAIGIDAAIAYSLVVVAVLMLPETKGRKME 410 >gi|300310615|ref|YP_003774707.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 478 Score = 93.2 bits (230), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 112/432 (25%), Positives = 175/432 (40%), Gaps = 56/432 (12%) Query: 24 DGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTSAIGGWAAGVLADRYGRVRVLQWT 83 D F + F++P L+ W +S A+ G + L IG G +A+RYGRV T Sbjct: 56 DAFHALSLAFVLPVLVGLWQLSAAQIGLLIASGYLGQVIGALFFGWMAERYGRVPSASVT 115 Query: 84 VLWFALFTFLSGFTTSFGELFFTRAMQGFGMGGEWAVGSVLIAETISARHRGKASGLVQS 143 V ++ + + T SF LF R +QG G+GGE V + I E A RGK L + Sbjct: 116 VGLMSVLSIVCALTGSFHMLFLCRFLQGIGVGGEVPVAATYINELSQAHGRGKFFVLYEL 175 Query: 144 SWAVGWAASALAFWGVYAVLPPETAWKVLFWIGVLPALLTLYIRRAISEPEVFLATQARL 203 + +G A+A V A L P W+ +F +G +P +L + + E +L + RL Sbjct: 176 IFPLGLLAAA----QVGAFLVPRFGWEYMFLVGGIPGILVALLITRLPESPRWLIARGRL 231 Query: 204 RESG--------------------------------------QRGNFLRIFHRDLLRTTL 225 ++ QRG++ +F T+ Sbjct: 232 DDAEKLIRTLEASTPKRNLDAQTDKQAIEQRINALAAGAGKRQRGSWKELFSPVYRGRTI 291 Query: 226 LASLLATGMQGAYYSVTTWLPTFLKTERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTI 285 + LL + WLP+ KT HL + + V S C L A L D + Sbjct: 292 IVWLLWASSYFVANGINNWLPSLYKTVYHLPLQESLRMASVSNVLSACAVLGCALLIDRV 351 Query: 286 GRRRCFMLFAAMGIVLVLSYTQLIITDAWMLALGFVLGFFLSGIFSGMGA-------YLS 338 GRRR + + L+ L AW + F S ++ MG Y Sbjct: 352 GRRRWAIASFILSGALLALLGVLKAESAWSVM------FLASSAYAVMGTTTVLLYLYTP 405 Query: 339 ELFPSDVRGSGQGFCYNFGRAVGAICPALVGYLSNSLPLGQTIGWIAAGCYGLVIVACLI 398 E++P+ +R G G ++ RA A PA+VG + + T+ + AG L ++A Sbjct: 406 EIYPTRMRAIGTGLATSWLRAASAAAPAMVGVVLAGHGIA-TVFLMFAGVTVLGLLAAWR 464 Query: 399 LPETRGKELEAV 410 + ET + LE + Sbjct: 465 MIETTNRSLEEI 476 >gi|300310449|ref|YP_003774541.1| 4-hydroxybenzoate transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 447 Score = 90.5 bits (223), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 103/386 (26%), Positives = 155/386 (40%), Gaps = 54/386 (13%) Query: 24 DGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTSAIGGWAAGVLADRYGRVRVLQWT 83 DGFD ++ P ++A W +SKA G + L +G + DRYGR VL + Sbjct: 41 DGFDVQAMGYVAPAIIADWGVSKASLGPVFGAGLFGMLLGSLVLTPVGDRYGRRPVLILS 100 Query: 84 VLWFALFTFLSGFTTSFGELFFTRAMQGFGMGGEWAVGSVLIAETISARHRGKASGLVQS 143 L+FAL + T+ +L R + GFG+G L+ E + R LV Sbjct: 101 TLFFALCMLATPMVTTLDQLLVLRFITGFGLGSIMPNAMALVGEFSPSSSRVTRMMLVSC 160 Query: 144 SWAVGWAASALAFWGVYAVLPPETAWKVLFWI-GVLPALLTLYIRRAISEPEVFLATQAR 202 + VG AA V A L P W +FW+ G +P +L L + + E FL R Sbjct: 161 GFTVGAAAGGF----VSAALIPAYGWHAVFWVGGAVPLVLGLAMLVWLPESIQFLVLHRR 216 Query: 203 LRESGQRGNFLRIFHRDLL---RTTL-----------LASLLATGMQGA----------- 237 R Q +LR +++ +T + +A+L G G Sbjct: 217 PRT--QVARWLRKLDPNIVIDDKTEVVVKETKAEGMPVAALFRDGRAGVTVLLWLISFMN 274 Query: 238 ---YYSVTTWLPTFLKTERHLSVLGTSTYLLVLIG-----GSFCGYLTSAWLSDTIGRRR 289 Y ++ WLPT ++ G ST + VLIG G G L+ G R Sbjct: 275 LINLYFLSNWLPTLIRDA------GYSTSMAVLIGTSLQVGGVIGTLSLGRFIQRFGFTR 328 Query: 290 ----CFMLFAAMGIVLVLSYTQLIITDAWMLALGFVLGFFLSGIFSGMGAYLSELFPSDV 345 CF+L A + I L+ +L A + V GF + G + A +P+ + Sbjct: 329 VLGSCFLL-ACLSIALI---GKLATVPALLFVAVIVAGFCIVGGQPAVNALAGTFYPTTL 384 Query: 346 RGSGQGFCYNFGRAVGAICPALVGYL 371 R +G G+ GR + P + G L Sbjct: 385 RSTGIGWALGIGRIGSVVGPVIGGQL 410 >gi|300310666|ref|YP_003774758.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 438 Score = 85.5 bits (210), Expect = 7e-18, Method: Compositional matrix adjust. Identities = 105/412 (25%), Positives = 179/412 (43%), Gaps = 35/412 (8%) Query: 24 DGFDYMIYTFLIPTLLAAWSMSKAEAGYIATGALLTSAIGGWAAGVLADRYGRVRVLQWT 83 D + F PT+ + ++ +A T + +G + G LADR GR VL Sbjct: 35 DAMQVLSIGFSAPTIAKTFGLTVPQALQTGTMFFVGMLLGAFVFGRLADRIGRRPVLMGA 94 Query: 84 VLWFALFTFLSGFTTSFGELFFTRAMQGFGMGGEWAVGSVLIAETISARHRGKASGLVQS 143 V+ A F S F F L R + G G+GG V ++AE + + RG+ +++S Sbjct: 95 VVIDACFGVASAFAPDFTWLLALRLLTGIGVGGTLPVDYTMMAEFLPSARRGRWLVMLES 154 Query: 144 SWAVGWAASALAFWGVYAVLPPETAWKVLFWIGVLPALLTLYIRRAISEPEVFL------ 197 WA+G LA + AV AW+V+F++ LPAL+ + +R + E ++L Sbjct: 155 FWAIG--TICLAVLALVAVSWGNDAWRVIFFVTGLPALVGVVLRFYVPESPMYLNRSGKS 212 Query: 198 --ATQARLRESGQRGNFLRI--FHRDLLRTTLLASLLATGMQG--------------AYY 239 A Q R + G+ I +++ +++L + ++ AYY Sbjct: 213 DAARQVLERVAKVNGSSTPIPPLQPEVVERKPMSALFSAALRRRSLSLYLAWGLISIAYY 272 Query: 240 SVTTWLPTFLKTERHLSVLGTSTYLLVLIGGSFCGYLTSAWLSDTIGRRRCFMLFAAMGI 299 V +LP L +E + +L+ L GY SA+ + GR+ + F + Sbjct: 273 GVFVYLPVKLGSE-GFGFMRGQIFLIFLALVQLPGYALSAYGVERWGRKPTLVGFLVLSA 331 Query: 300 VLVLSYTQLIITDAWMLALGFVLGFFLSGIFSGMGAYLSELFPSDVRGSGQGFCYNFGRA 359 V + Y+ L ++ +L F L G ++ + A+ E++P+D+R SG G R Sbjct: 332 VGCMLYS-LGKDPMLVVGSTLLLSFSLLGTWAALYAFTPEVYPTDLRASGMGTAGAVARF 390 Query: 360 VGAICPALVGYLSNS---LPLGQTIGWIAAGCYGLVIVACLILPETRGKELE 408 G PA+V + S L L ++ AG ++ V E+R + LE Sbjct: 391 GGLFAPAIVAPVMASHFTLALAMLSAFLVAGAVAILCVDV----ESRNRALE 438 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.139 0.444 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,307,453 Number of extensions: 56334 Number of successful extensions: 382 Number of sequences better than 1.0e-08: 25 Number of HSP's gapped: 330 Number of HSP's successfully gapped: 26 Length of query: 414 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 326 Effective length of database: 1,203,190 Effective search space: 392239940 Effective search space used: 392239940 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)