BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0221.p (300 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312785|ref|YP_003776877.1| transcription regulator protein... 506 e-144 gi|300310322|ref|YP_003774414.1| LysR family transcription regul... 126 2e-30 gi|300312373|ref|YP_003776465.1| LysR family transcription regul... 122 5e-29 gi|300310059|ref|YP_003774151.1| LysR family transcription regul... 107 1e-24 gi|300312300|ref|YP_003776392.1| LysR family transcription regul... 106 3e-24 >gi|300312785|ref|YP_003776877.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 297 Score = 506 bits (1303), Expect = e-144, Method: Compositional matrix adjust. Identities = 254/297 (85%), Positives = 271/297 (91%) Query: 4 LADIEPNWEWYRSFLKVLETGSLSAAGREMGLTQPTVGRHIEQLESALGLKLFVRSFDGF 63 +ADIEPNWEWYRSFLKVLETGSLSAAGREMGLTQPTVGRHIE LES LGLKLFVRSFDGF Sbjct: 1 MADIEPNWEWYRSFLKVLETGSLSAAGREMGLTQPTVGRHIENLESTLGLKLFVRSFDGF 60 Query: 64 SPTDAALELRPYAAGIAATSAALRRVASSHGREVRGTVRLTASEVISVEVLPPILAELKH 123 SPTDAALEL+PYAA IAA SAALRR ASS G+EVRGTVRLTASEVISVEVLPPILA L+H Sbjct: 61 SPTDAALELKPYAAAIAANSAALRRAASSQGKEVRGTVRLTASEVISVEVLPPILAALRH 120 Query: 124 RHPGLCIELVMSNRADDLLQREADIAVRMFKPVQDALIAKRLGSIEVGLHAHQRYLAAHG 183 RHP L +ELV+SN+ADDLLQREADIAVRMF+P QDAL+AKRLGSIE+GLHAHQ YL HG Sbjct: 121 RHPELRVELVLSNKADDLLQREADIAVRMFRPAQDALVAKRLGSIELGLHAHQSYLTVHG 180 Query: 184 VPHSIDALAGHALIGFDQESAFVRKVLEQFPWLARAGLAFRADSDLAQLAAIRAGFGIGI 243 P S+DALA H LIGFDQESA+VR VL+Q PWL+RA LA R DSDLAQLAAIRAGFGIGI Sbjct: 181 APKSVDALADHTLIGFDQESAYVRNVLKQIPWLSRAALALRTDSDLAQLAAIRAGFGIGI 240 Query: 244 CQVGLAARDDALVHLLPRKFSLKMETWIAMHEDLRASPRCAVTFAALVAGLGAYIRG 300 CQV LAARD ALV LLPR+F+LKMETW+AMHEDLRASPRCAVTFAALVAGLGAYIR Sbjct: 241 CQVALAARDVALVRLLPRQFALKMETWVAMHEDLRASPRCAVTFAALVAGLGAYIRS 297 >gi|300310322|ref|YP_003774414.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 288 Score = 126 bits (316), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 81/287 (28%), Positives = 138/287 (48%), Gaps = 1/287 (0%) Query: 10 NWEWYRSFLKVLETGSLSAAGREMGLTQPTVGRHIEQLESALGLKLFVRSFDGFSPTDAA 69 +W+ R FL + G+L A + L Q TVGR + LE +LG +LF+R+ +G+ PT A Sbjct: 2 DWDNARIFLAIYRVGTLRGAAETLELDQATVGRRLAALERSLGARLFLRTPNGYVPTPAG 61 Query: 70 LELRPYAAGIAATSAALRRVASSHGREVRGTVRLTASEVISVEVLPPILAELKHRHPGLC 129 A + + L+R + G VRL A++V + L P L L+ HP + Sbjct: 62 EMAAAAAQQMEQGAEQLQRQMQGIDERLCGVVRLAATDVTARHFLIPALHRLRQEHPHIR 121 Query: 130 IELVMSNRADDLLQREADIAVRMFKPVQDALIAKRLGSIEVGLHAHQRYLAAHGVPHSID 189 + L S + +L +READIAVR +P LIA+ L + ++A + Y+ G+P Sbjct: 122 VVLSTSTQITNLTKREADIAVRTLRPDAPDLIARHLKRLHTAIYASRSYVKKRGLPEPGT 181 Query: 190 ALAGHALIGFDQESAFVRKVLEQFPWLARAGLAFRADSDLAQLAAIRAGFGIGICQVGLA 249 L GH ++ + + + + + A +A + L ++AGFG+G + Sbjct: 182 GLKGHDVVIYQRSVSPAKSEQICGESIINAQVAMEVSTGLVMYDCLQAGFGLGELPTHVG 241 Query: 250 ARDDALVHLLPRKFSLKMETWIAMHEDLRASPRCAVTFAALVAGLGA 296 + LV + P++ + + W+ +H DL + R A++A A Sbjct: 242 DKASGLVRVWPQRET-PYDMWLVLHSDLNRTARVRAVADAIIAAYEA 287 >gi|300312373|ref|YP_003776465.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 305 Score = 122 bits (305), Expect = 5e-29, Method: Compositional matrix adjust. Identities = 81/290 (27%), Positives = 142/290 (48%), Gaps = 8/290 (2%) Query: 10 NWEWYRSFLKVLETGSLSAAGREMGLTQPTVGRHIEQLESALGLKLFVRSFDGFSPTDAA 69 +W+ R FL++ + L A +++G+ TV R +++ E +G +LF R+ G+ T Sbjct: 3 DWDNLRIFLELTRSQGLVDAAKKLGIDHSTVSRRMKRFEEQVGSQLFDRNNHGYKLTADG 62 Query: 70 LELRPYAAGIAATSAALRRVASSHGREVRGTVRLTASEVISVEVLPPILAELKHRHPGLC 129 L YA + +T A H ++ G VRL A+E VL P +A R+P + Sbjct: 63 YRLVEYAEQVESTLYAATEELGGHNSKLSGQVRLGATEGFGTFVLAPHIAHFCARNPHIT 122 Query: 130 IELVMSNRADDLLQREADIAVRMFKPVQDALIAKRLGSIEVGLHAHQRYLAAHGVPHSID 189 ++++ R +L + EADI+V + +P+ + +L + L+A + YLA H H++D Sbjct: 123 VDMLPMPRFVNLSKHEADISVSIERPLSGNYVVTKLSDYVLKLYASRDYLAQHPPIHTMD 182 Query: 190 ALAGHALIGFDQESAFVRKVLEQFPWLARAGLAFRADSDLAQLAAIRAGFGIGICQVGLA 249 LA H IG+ + F ++ + A AFR+ S +AQ A AG + I LA Sbjct: 183 DLAQHHFIGYIDDLVFSEELRYKDTVAPDAFRAFRSTSVVAQYTAACAGRALAILPCFLA 242 Query: 250 ARDDALVHLLPRKFSLKMETWIAMHEDLRASPRCAVTFAALVAGLGAYIR 299 + + L +LP++ ++ W+ + R R V+ L +Y+R Sbjct: 243 QQSEELAPVLPQQIAITRSFWLVASSEQRHVAR--------VSALWSYLR 284 >gi|300310059|ref|YP_003774151.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 283 Score = 107 bits (268), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 88/290 (30%), Positives = 128/290 (44%), Gaps = 16/290 (5%) Query: 10 NWEWYRSFLKVLETGSLSAAGREMGLTQPTVGRHIEQLESALGLKLFVRSFDGFSPTDAA 69 +WE RSF ++ GS S A R +G+T T+GR ++QLE L LF+R D T Sbjct: 4 DWESLRSFAAFVDAGSFSGAARVLGVTHATIGRRLQQLEQTLAAPLFIRRADDIELTRLG 63 Query: 70 LELRPYAAGIAATSAALRRVASSHGREVRGTVRLTASEVISVEVLPPILAELKHRHPGLC 129 + A + + L R + + G +RL ++ + L P L L + P L Sbjct: 64 ESVLAAARAMQDQTRQLARTLAGEDLRMEGKLRLACTDAMGTLFLAPRLPSLLQQWPALD 123 Query: 130 IELVMSNRADDLLQREADIAVRMFKPVQDALIAKRLGSIEVGLHAHQRYLAAHGVPHSID 189 IEL M ++ L +READ+A+R +P LIA+RLG + L +AA Sbjct: 124 IELAMGHQTLSLARREADLALRFARPQDGELIARRLGVVRFYLCGTADQVAAWRHQR--- 180 Query: 190 ALAGHALIGFDQESAFVRKVLEQFPWLARAG----LAFRADSDLAQLAAIRAGFGIGICQ 245 +IG+D + + WLA + R+ S +AQ A RAG G+ + Sbjct: 181 --GSAPVIGYDDSVPDI----PETRWLAAHADGNPVRLRSASLVAQCMAARAGVGLALLP 234 Query: 246 VGLAARDDALVHLLPRKFSLKMETWIAMHEDLRASPRCAVTFAALVAGLG 295 L A + V P+ L E W H DL+A PR L A G Sbjct: 235 SYLLAPELQTVESTPQ---LVRELWAVFHRDLKALPRLRAVLEWLQACCG 281 >gi|300312300|ref|YP_003776392.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 305 Score = 106 bits (264), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 86/296 (29%), Positives = 139/296 (46%), Gaps = 25/296 (8%) Query: 1 MNTLADIEPNWEWYRSFLKVLETGSLSAAGREMGLTQPTVGRHIEQLESALGLKLFVRSF 60 MNTL W+ R FL + GSLSA+ R + + TV RH+ LE L +KLF R Sbjct: 20 MNTL-----QWDDVRYFLTLSREGSLSASARVLKVEHSTVARHVGNLEKTLRVKLFDRLA 74 Query: 61 DGFSPTDAALELRPYAAGIAATSAALRRVASSHGREVRGTVRLTASEVISVEVLPPILAE 120 G+ T EL A + +L R A ++ + G VR++A ++ + L P L Sbjct: 75 RGWVLTTEGAELSKQAEQLEVEMHSLWRAALANT-TLSGKVRVSAPPILMSDFLVPRLPA 133 Query: 121 LKHRHPGLCIELVMSNRADDLLQREADIAVRMFKPVQDALIAKRLGSIEVGLHAHQRYLA 180 + ++P + +E++ R DL + EADIA+RM +PV L+A+ L ++ + Sbjct: 134 FQEKYPDIELEVIGEARGVDLTRGEADIALRMGEPVSPLLVARMLCNVSYSV-------- 185 Query: 181 AHGVPHSID-ALAGHALIGFDQESAFVRKVLEQFPWLA----RAGLAFRADSDLAQLAAI 235 +G+P ++ + + IG DQES +K WL + A R + A A Sbjct: 186 -YGIPAWLERPESERSFIGLDQESPCRQKA-----WLESQVDQRRYALRTNDIRAMCHAA 239 Query: 236 RAGFGIGICQVGLAARDDALVHLLPRKFSLKMETWIAMHEDLRASPRCAVTFAALV 291 +G G+ + + A D LV + + ++ MH D+R +PR T L+ Sbjct: 240 MSGIGLALLPRFMVAEDTRLVAIPMPETPPPCPLYLVMHSDVRRAPRIRATADFLI 295 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.137 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 948,762 Number of extensions: 39923 Number of successful extensions: 319 Number of sequences better than 1.0e-08: 44 Number of HSP's gapped: 315 Number of HSP's successfully gapped: 44 Length of query: 300 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 215 Effective length of database: 1,217,395 Effective search space: 261739925 Effective search space used: 261739925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 130 (54.7 bits)