BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0209.p (363 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310800|ref|YP_003774892.1| tartrate dehydrogenase [Herbasp... 315 4e-87 gi|300312594|ref|YP_003776686.1| 3-isopropylmalate dehydrogenase... 160 1e-40 gi|300310728|ref|YP_003774820.1| isocitrate dehydrogenase [Herba... 100 1e-22 >gi|300310800|ref|YP_003774892.1| tartrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 315 bits (806), Expect = 4e-87, Method: Compositional matrix adjust. Identities = 165/360 (45%), Positives = 229/360 (63%), Gaps = 15/360 (4%) Query: 2 KTYRIATIPGDGIGKEVIPAGRQLLQALAAARADLAFDFEDFGWGG-DYYRQHGVMMPAD 60 KT+RIA I GDGIG+EV+P G + +QA A+ + + +F F W +YY +HG MMP D Sbjct: 3 KTHRIAVIAGDGIGQEVMPEGLRAVQA-ASKKFGIDIEFTHFDWAHCNYYAEHGKMMPDD 61 Query: 61 GLDALRDKDAILFGSAGDP-HIPDHVTLWGLRLKICQGFDQYANVRPTRILPGIDAPLKR 119 + L+ +AI FG+ G P +PDHV+LWG LK + FD Y N+RP R++PG+ PL Sbjct: 62 WFEQLKGFEAIYFGAVGWPATVPDHVSLWGSLLKFRRDFDLYVNLRPVRLMPGVPCPLAN 121 Query: 120 CRAEDLNWVIVRENSEGEYAGVGGRVHQGHPIEAATDVSMMTRAGVERIMRFAFKLAQSR 179 + D+++ +VREN+EGEY+ +GGR+++G E S+ +R G +RI++FAF LAQSR Sbjct: 122 RKPGDIDFYVVRENTEGEYSSIGGRMYEGTERETVIQESVFSRKGTDRILKFAFDLAQSR 181 Query: 180 PRKLLTVVTKSNAQRYAMVMWDEIAVQIAKEFPDVKWDKELVDAATARMVNRPATLDTIV 239 P+K LT TKSN M WDE + ++PDV+ DK +D TA V P D +V Sbjct: 182 PKKHLTSATKSNGIAITMPYWDERVEAMGAKYPDVRRDKYHIDILTAHFVLNPDRFDVVV 241 Query: 240 ATNLHADILSDLAAALAGSLGIAPTGNIDPECRYPSMFEPIHGSAFDIMGKGLANPVGTF 299 A+NL DILSDL A AG++GIAP+ NI+P PS+FEP+HGSA DI GK +ANPV Sbjct: 242 ASNLFGDILSDLGPACAGTIGIAPSANINPTRELPSLFEPVHGSAPDIFGKNIANPVAMI 301 Query: 300 WSVVMLLEHLGE--------HDAAAQVMQAIEAVTADPALHTGDLGGKATTVQVTEAVCS 351 WS M+L+ LG+ HDA ++ AIE V + T DLGGK+ T +V +A+ + Sbjct: 302 WSGAMMLDFLGDGQGKYRQAHDA---MLAAIETVLVN-GPTTPDLGGKSNTAEVGQAIAA 357 >gi|300312594|ref|YP_003776686.1| 3-isopropylmalate dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 356 Score = 160 bits (406), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 125/370 (33%), Positives = 182/370 (49%), Gaps = 35/370 (9%) Query: 5 RIATIPGDGIGKEVIPAGRQLLQALAAARADLAFDFEDFGWGGDYYRQHGVMMPADGLDA 64 +IA +PGDGIG E++ ++L AL D F+ E GG Y HG +P L Sbjct: 2 KIAILPGDGIGPEIVNEAVRVLNAL-----DEKFEMETAPVGGAGYEAHGHPLPDGTLKL 56 Query: 65 LRDKDAILFGSAGDPHI--------PDHVTLWGLRLKICQGFDQYANVRPTRILPGID-- 114 ++ DAILFG+ GD P+ L GLR + +AN RP P + Sbjct: 57 AKEADAILFGAVGDWKYDKLERALRPEQAIL-GLR----KHLQLFANFRPAICYPELTGA 111 Query: 115 APLKRCRAEDLNWVIVRENSEGEYAGV--GGRVHQGHPIEAAT---DVSMMTRAGVERIM 169 + LK +L+ +IVRE + Y G G R P + A D + + RI Sbjct: 112 SSLKPELVANLDILIVRELNGDIYFGQPRGMREAPDGPFKGAREGFDTMRYSEPEIRRIA 171 Query: 170 RFAFKLAQSRPRKLLTVVTKSNAQRYAMVMWDEIAVQIAKEFPDVKWDKELVDAATARMV 229 AF+ A R ++L +V K+N W +I ++ KE+PDV+ VD A ++V Sbjct: 172 HVAFQAAAKRGKRLCSV-DKANVLE-TFQFWKDIVTEVGKEYPDVELSHMYVDNAAMQLV 229 Query: 230 NRPATLDTIVATNLHADILSDLAAALAGSLGIAPTGNIDPECRYPSMFEPIHGSAFDIMG 289 P D IV N+ DILSD AA L GS+G+ P+ ++D + ++EP HGSA DI G Sbjct: 230 KAPKNFDVIVTGNMFGDILSDAAAMLTGSIGMLPSASLDANNK--GLYEPSHGSAPDIAG 287 Query: 290 KGLANPVGTFWSVVMLLE-HLGEHDAAAQVMQAIEAVTADPALHTGDL----GGKATTVQ 344 +G+ANP+ T S M+L L + + A ++ A++ V A L T D+ K T Q Sbjct: 288 RGIANPLATILSAAMMLRFSLNKAEQADRIENAVKKVLAQ-GLRTADIYEEGCTKVNTQQ 346 Query: 345 VTEAVCSFVA 354 + EAV +A Sbjct: 347 MGEAVVKALA 356 >gi|300310728|ref|YP_003774820.1| isocitrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 418 Score = 100 bits (250), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 98/364 (26%), Positives = 152/364 (41%), Gaps = 57/364 (15%) Query: 6 IATIPGDGIGKEVIPAGRQLLQALAAARADLAFDFEDFGW-----GGDYYRQHG--VMMP 58 I I GDG+G +V P +L+ + AA W G R +G V +P Sbjct: 31 IPYIVGDGVGVDVTPV---MLKVVNAAVEKAYGGARKIHWMEIYAGEKATRLYGPDVWLP 87 Query: 59 ADGLDALRDKDAILFGSAGDPHIPDHVTLWGLRLKICQGFDQYANVRPTRILPGIDAPLK 118 + L L+ L G P + L + + Q D Y +RP R G+ +PL+ Sbjct: 88 EETLAVLKK---YLVAIKGPLSTPVGGGIRSLNVAMRQQLDLYVCLRPVRYFKGVPSPLR 144 Query: 119 RCRAEDLNWVIVRENSEGEYAGVG-------------------GRVHQGHPIEAATDVSM 159 D+ VI RENSE YAG+ G P +A + Sbjct: 145 EPEKTDM--VIFRENSEDIYAGIEWAAGTPEVNKLIDLLTREMGVKKLRFPESSALGIKP 202 Query: 160 MTRAGVERIMRFAFKLAQSRPRKLLTVVTKSNAQRYAMVMWDEIAVQIA-KEF-----PD 213 ++R G ER++R A + A + +T+V K N ++ + + +A +EF D Sbjct: 203 VSREGTERLVRQAIQYAIDHDKPSVTLVHKGNIMKFTEGAFRDWGYALAAREFGAELIDD 262 Query: 214 VKWDK--------------ELVDAATARMVNRPATLDTIVATNLHADILSDLAAALAGSL 259 W + + DA +++ RPA I NL+ D +SD AA G + Sbjct: 263 GPWMRLKNPKTGRAIIIKDAITDAFFQQVLMRPAEYSVIATLNLNGDYISDAVAAQVGGI 322 Query: 260 GIAPTGNIDPECRYPSMFEPIHGSAFDIMGKGLANPVGTFWSVVMLLEHLGEHDAAAQVM 319 GIAP N+ ++FE HG+A GK NP + S M+L H+G +AA ++ Sbjct: 323 GIAPGANMSDSV---AVFEATHGTAPKYAGKDYVNPGSSILSAEMMLRHMGWIEAADLII 379 Query: 320 QAIE 323 A++ Sbjct: 380 SAMQ 383 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,074,882 Number of extensions: 42867 Number of successful extensions: 143 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 132 Number of HSP's successfully gapped: 3 Length of query: 363 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 277 Effective length of database: 1,212,660 Effective search space: 335906820 Effective search space used: 335906820 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)