BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0203.p (422 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310486|ref|YP_003774578.1| major facilitator superfamily p... 72 8e-14 gi|300310805|ref|YP_003774897.1| major facilitator superfamily p... 68 1e-12 gi|300310608|ref|YP_003774700.1| major facilitator superfamily p... 57 2e-09 >gi|300310486|ref|YP_003774578.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 430 Score = 72.0 bits (175), Expect = 8e-14, Method: Compositional matrix adjust. Identities = 101/435 (23%), Positives = 188/435 (43%), Gaps = 43/435 (9%) Query: 1 MTTLTQTAPASAQGLLRNRWFQLLIGLICMMAISSPQYVWTLFTKPLTAKLGVPLSELQI 60 MT TQ+ + W + + + ++ + + ++ PL + G S + + Sbjct: 1 MTKFTQSLANAVTPRFHYAWVVVGVIFLVLLCSAGIRATPSVMILPLEQEFGWNRSTISV 60 Query: 61 TFSMLIVLQTFFSPFQGSLIERFGARRLIAIGTFMSGLSWILAAQASTIGMFYLAYGLVG 120 S+ I L PF + ++RFG RR++ + L+ L++GL+ Sbjct: 61 VISVNIALYGLIGPFSAAAMQRFGIRRVVLAALVLLASGTALSTLMHMPWHMLLSWGLLV 120 Query: 121 GLGTGIVYVGVVGQMV-RWFPDRRGFAVGMVAAGYGMGAILTTFPISASLASAGLETTLW 179 G GTG+ + +V RWF RRG A+G++ A G ++ ++A + S G + Sbjct: 121 GAGTGVAANTLGATIVSRWFETRRGLAMGLLTASAATGQMVFLPLMAAMVGSYGWRSV-- 178 Query: 180 QFGLVFAVIGVLAAQAL-----RSPDA------SHAAAMPAPAIAASK-----LD-LRQM 222 + + + +LA + SP A + + ++ LD LR Sbjct: 179 --AFLVSAVALLAIPLVWLLLPESPAAIGQKMLGQTSEIKDEGLSRRNPLFIALDALRTS 236 Query: 223 LRTPLFWLMFV--MMTMMSTSGLMVTSQMATFARDFGVANVMVFG-LAALPLALTIDRLT 279 +R P FWL+F + +ST+G + +Q D+G+ V LAA+ + +D + Sbjct: 237 VRMPDFWLLFASFFVCGLSTNG-YIGTQFIAMCNDYGINEVSSASILAAMGM---LDLVG 292 Query: 280 NGLTRPFFGWISDRYGRENTMFFAFALEGVAMALWLATRENAVLFVLLSGIVFFGWGEIF 339 L+ GW+SDRY +F+ + L G+A+ ++L + L +F+G I Sbjct: 293 TTLS----GWLSDRYNPRVLLFWYYGLRGLAL-IFLPYAFGLQYYGLTIFAIFYGLDWIA 347 Query: 340 SLFPST--LTDTFGTRNATANYGWLYISQGIGSIFGGPLAAMMHEKTGSWHPVFGTAITL 397 ++ P+ D FG A +GW+ +G+ LA + G++ T ++ Sbjct: 348 TVPPTVRLANDVFGRLAAPIVFGWIVAGHQLGAATATTLAGYLRASLGNY-----TLSSI 402 Query: 398 DILTACL--ALMVLK 410 + ACL A++VL+ Sbjct: 403 LMGVACLVGAVLVLR 417 >gi|300310805|ref|YP_003774897.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 580 Score = 68.2 bits (165), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 100/383 (26%), Positives = 164/383 (42%), Gaps = 53/383 (13%) Query: 43 FTKPLTAKLGVPLSELQITFSMLIVLQTFFSPFQGSLIERFGARR--LIAIGTFMSGL-S 99 F + ++ +S L ++M VL + G +E G R+ ++A + GL Sbjct: 97 FAQVFSSSCDWKISMLGWMYTMFFVLLGISAALWGGWLEHAGPRKAGVVAAICWCGGLLI 156 Query: 100 WILAAQASTIGMFYLAYGLVGGLGTGIVYVGVVGQMVRWFPDRRGFAVGMVAAGYGMGAI 159 L + I + +L G++GG+G G+ Y+ V +++WFPDRRG A GM G+G GA+ Sbjct: 157 SALGVKTHQIWLMWLGSGVIGGVGLGLGYISPVSTLIKWFPDRRGMATGMAIMGFGGGAL 216 Query: 160 LTTFPISASLASAGLETT--LWQFGLVFAVIGVL----AAQALRSPDASHAAAMPAPAIA 213 + T A++ T+ +W+ +V AVI + A R P A P +A Sbjct: 217 VGTPLADAAMKKFATPTSVGVWETFVVMAVIYFVFMMAGAFGYRVPPTGWKPAGWTPPVA 276 Query: 214 AS--------KLDLRQMLRTPLFWLMFVMMTMMSTSGLMVTSQMATFARDFGVANVM--- 262 S + + ++ P FWL+++++ + ++G+ V G+A+ M Sbjct: 277 KSSATMITQNHVHVSKVWGIPQFWLVWLVLCLNVSAGIGV----------IGIASPMLQE 326 Query: 263 VFGLAALPLALTIDRLT--------------NGLTRPF-----FGW--ISDRYGRENT-- 299 VFG L + + + L+ GL F F W SD +GR+ T Sbjct: 327 VFGGKLLGVNIGFNDLSVEQKTAIAALAAGFTGLISLFNIGGRFAWASCSDFFGRKMTYA 386 Query: 300 MFFAFALEGVAMALWLATRENAVLFVLLSGIVFFGWGEIFSLFPSTLTDTFGTRNATANY 359 +FF W A N LFV I+ +G F+ P+ L D FGT+ A + Sbjct: 387 IFFVLGFVLYVSLPWSAKAGNIPLFVGAVCIILSMYGGGFATVPAYLADLFGTQMVGAIH 446 Query: 360 GWLYISQGIGSIFGGPLAAMMHE 382 G L + I G + M E Sbjct: 447 GRLLTAWATAGILGPVVVNYMRE 469 >gi|300310608|ref|YP_003774700.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 407 Score = 57.4 bits (137), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 94/401 (23%), Positives = 162/401 (40%), Gaps = 33/401 (8%) Query: 17 RNRWFQLLIGLICM-MAISSPQYVWTLFTKPLTAKLGVPLSELQITFSMLIVLQTFFSPF 75 R W +L G M MA+ S Q + LF PL+ G+P++ + ++ ++ Sbjct: 8 RKPWLVVLAGAAIMSMALGSRQ-TFGLFISPLSYDHGLPVTLVAFAIALHNLVWGVAQSL 66 Query: 76 QGSLIERFGARRLIAIGTFMSGLSWILAAQASTIGMFYLAYGLVGGLGTGIVYVGVVGQM 135 G+L +R G ++A G + L A A + L G+ G+G GVV Sbjct: 67 AGALTDRHGPAPVVAFGAVCYAIGLALTALAPNGAVLVLGMGVFIGVGISCTSFGVVLPA 126 Query: 136 V-RWFP-DRRGFAVGMVAAGYGMGAILTTFPISASLASAGLETTLWQF----------GL 183 V R P +RR A+G+V+AG +G +L + A++G+ L GL Sbjct: 127 VGRSVPAERRSSAMGLVSAGGSLGQVLMLPLVQGLRATSGMSLALMVLALLVLAVAPLGL 186 Query: 184 VFAVIGVLAAQALRSPDASHAAAMPAPAIAASKLDLRQMLRTPLFWLMFVMMTMMSTSGL 243 A G +AA A R +R+++ + ++T+ + Sbjct: 187 ALARTGPVAADAPRL-----------------DYSVREVIAQATAHRGYQLLTLGFFACG 229 Query: 244 MVTSQMATFARDFGVANVMVFGLAALPLALTIDRLTNGLTRPFFGWISDRYGRENTMFFA 303 + +AT + M GLAA LAL L N GW+ RY + + + Sbjct: 230 FQLAFIATHLPGYLELCHMPMGLAATALALI--GLFNMAGSWACGWLGGRYRPQRVLGWL 287 Query: 304 FALEGVAMALWLATRENAVLFVLLSGIVFFGWGEIFSLFPSTLTDTFGTRNATANYGWLY 363 + + A+ L+ +++ L + ++ W L + FGT + +G + Sbjct: 288 YLVRSTAIGLFFIAPKSSQSVTLFAAVMGLTWLGTVPLTTGLVARLFGTAHMGTLFGVCF 347 Query: 364 ISQGIGSIFGGPLAAMMHEKTGSWHPVFGTAITLDILTACL 404 +S +GS G L M E TGS+ ++ + I+ A L Sbjct: 348 LSHQVGSFLGAWLGGWMLEVTGSYSLLWMATFVVGIVAALL 388 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.139 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,228,560 Number of extensions: 49006 Number of successful extensions: 330 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 319 Number of HSP's successfully gapped: 4 Length of query: 422 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 334 Effective length of database: 1,203,190 Effective search space: 401865460 Effective search space used: 401865460 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)