BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0191.p (400 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312624|ref|YP_003776716.1| acetylornithine aminotransferas... 793 0.0 gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferas... 166 3e-42 gi|300312816|ref|YP_003776908.1| aminotransferase class III prot... 143 2e-35 gi|300313229|ref|YP_003777321.1| 4-aminobutyrate aminotransferas... 140 1e-34 gi|300310456|ref|YP_003774548.1| family II aminotransferase [Her... 125 8e-30 >gi|300312624|ref|YP_003776716.1| acetylornithine aminotransferase [Herbaspirillum seropedicae SmR1] Length = 400 Score = 793 bits (2047), Expect = 0.0, Method: Compositional matrix adjust. Identities = 383/400 (95%), Positives = 392/400 (98%) Query: 1 MQYSQSDTNKLMYVTNRPELVFTEGHGMWLTDHNGKRYLDYLQGWAVNTLGHSPQCIADA 60 MQYSQSD NKLMYVTNRPELVFTEGHGMWLTDHNGKRYLDYLQGWAVNTLGH+PQCIADA Sbjct: 1 MQYSQSDANKLMYVTNRPELVFTEGHGMWLTDHNGKRYLDYLQGWAVNTLGHAPQCIADA 60 Query: 61 LNAQSKKLINPSPAFYNAPSMELAELLTANSVFDRVFFANSGAEANEGAIKLARKWGKKN 120 L AQSKKLINPSPAFYN PS+ELA+LLTANSVFDRVFFANSG EANEGAIKLARKWGKKN Sbjct: 61 LAAQSKKLINPSPAFYNEPSIELAKLLTANSVFDRVFFANSGGEANEGAIKLARKWGKKN 120 Query: 121 PAADGSSRFEIITFKHSFHGRTLATMSASGKDGWDTMFAPQVPGFPKAILNDLASVEALI 180 PAADGS+RFEIITFKHSFHGRTLATMSASGKDGWDTMFAPQVPGFPKA+LNDL SV+ALI Sbjct: 121 PAADGSARFEIITFKHSFHGRTLATMSASGKDGWDTMFAPQVPGFPKAVLNDLESVKALI 180 Query: 181 GSHTVAVMLEPVQGEGGVIPATKEFMQGLRRLTKEKNLLLIVDEVQAGMGRTGQLFAYQH 240 G HTVAVMLEPVQGEGGVIPA+KEFMQGLR LTKEKNLLLIVDEVQ+GMGRTGQLFAYQH Sbjct: 181 GEHTVAVMLEPVQGEGGVIPASKEFMQGLRSLTKEKNLLLIVDEVQSGMGRTGQLFAYQH 240 Query: 241 SGIEPDIMTLAKGIGGGVPLAALLAREEIACFEAGEQGGTYNGNPLMTAVGVAVIKELLK 300 SGIEPDIMTLAKGIGGGVPLAALLAREEIACFEAGEQGGTYNGNPLMTAVGVAVIKELLK Sbjct: 241 SGIEPDIMTLAKGIGGGVPLAALLAREEIACFEAGEQGGTYNGNPLMTAVGVAVIKELLK 300 Query: 301 PGFMESVRERGQYLRQRSLEISEKYGFEGERGEGLLRALQLGRDIGPQIVEAARNLEPVG 360 PGFMESVRERGQYLRQRSLEISEKYGFEGERGEGLLRALQLGRDIGPQIVEAARNLEPVG Sbjct: 301 PGFMESVRERGQYLRQRSLEISEKYGFEGERGEGLLRALQLGRDIGPQIVEAARNLEPVG 360 Query: 361 LLLNSPRPNLLRFMPALNVTKEEIDQMFSMLEDVLAKIEK 400 LLLNSPRPNLLRFMPALNVTKEEIDQMFSMLE+VLAKI K Sbjct: 361 LLLNSPRPNLLRFMPALNVTKEEIDQMFSMLEEVLAKIGK 400 >gi|300310415|ref|YP_003774507.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 426 Score = 166 bits (420), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 134/398 (33%), Positives = 200/398 (50%), Gaps = 42/398 (10%) Query: 28 MWLTDHNGKRYLDYLQGWAVNTLGHSPQCIADALNAQSKKLINPSPAFY-NAPSMELAEL 86 +W D G+R++D+ G AV GH + DA+ AQ K + + A +ELAE Sbjct: 38 LW--DVEGRRFIDFAAGIAVLNTGHRHPKLLDAMRAQMDKFTHTAYQIVPYASYVELAER 95 Query: 87 ---LTANSVFDRVFFANSGAEANEGAIKLARKWGKKNPAADGSSRFEIITFKHSFHGRTL 143 LT + + F ++GAEA E AIK+AR + R +I F FHGRT+ Sbjct: 96 INRLTPGNYPKKTAFFSTGAEAVENAIKIARAH---------TGRPGVIAFAGGFHGRTM 146 Query: 144 ATMSASGKDG-WDTMFAPQVPG------FPKAIL-----NDLASVEALIGS-----HTVA 186 M+ +GK + F P PG +P A+ + L +V+ L S A Sbjct: 147 MGMALTGKVAPYKLGFGP-FPGDVFHAPYPSALHGITSEDALEAVKGLFKSDIEAKRVAA 205 Query: 187 VMLEPVQGEGGVIPATKEFMQGLRRLTKEKNLLLIVDEVQAGMGRTGQLFAYQHSGIEPD 246 ++LEPVQGEGG A +FM+GLR L E +LLI DEVQ+G GRTG+LFA +H + PD Sbjct: 206 IILEPVQGEGGFYAAPADFMRGLRALCDEHGILLIADEVQSGYGRTGKLFAMEHYDVLPD 265 Query: 247 IMTLAKGIGGGVPLAALLAREEIACFEA-GEQGGTYNGNPLMTAVGVAVIKELLKPGFME 305 +MT+AK + GG+PL+A+ R EI A G GGTY GNPL A +AV+ + + + Sbjct: 266 LMTMAKSLAGGMPLSAVNGRAEIMDAPAPGGLGGTYAGNPLAIASALAVLDVMEEEQLVT 325 Query: 306 SVRERGQYLRQRSLEISEKYGFEGE-RGEGLLRALQL-----GRDIGPQIVEAARNLEPV 359 + G L++ E+ E RG G + A++ G+ + ++ Sbjct: 326 RGQRLGDKLQEHLKELRSSVPQIAEVRGVGAMVAVEFADPATGKPDAEYTKKVQQHALNN 385 Query: 360 GLLLNS--PRPNLLRFMPALNVTKEEIDQMFSMLEDVL 395 GLLL + N++RF+ L + +D+ +L + Sbjct: 386 GLLLLTCGSYGNVIRFLFPLTIPDTVMDEALGILAKAI 423 >gi|300312816|ref|YP_003776908.1| aminotransferase class III protein [Herbaspirillum seropedicae SmR1] Length = 440 Score = 143 bits (361), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 122/424 (28%), Positives = 198/424 (46%), Gaps = 58/424 (13%) Query: 25 GHGMWLTDHNGKRYLDYLQGWAVNTLGHSPQCIADALNAQSKKLINPSPAFY-NAPSMEL 83 G GM L D +GKRY+D G AV+ LGH + +A+ Q +L +F+ AP+ EL Sbjct: 19 GEGMELIDQDGKRYIDASGGAAVSCLGHGHPRVIEAIRKQVGELAYAHTSFFTTAPAEEL 78 Query: 84 AELL--TANSVFDRVFFANSGAEANEGAIKLARKWGKKNPAADGSSRFEIITFKHSFHGR 141 A +L A + V+F + G+EA E A+KLAR++ + R II + S+HG Sbjct: 79 AAMLADAAPGSLNHVYFLSGGSEAVEAALKLARQYYVE---VGQPQRRHIIARRQSYHGN 135 Query: 142 TLATMSASGKDGWDTMFAPQV-------PGFPKAILNDLAS---------------VEAL 179 TL ++ G MF P + P + D S + +L Sbjct: 136 TLGALAIGGNAWRREMFMPMLIEAHHVSPCYAYRNRADGESDVQYVQRLADELEQKILSL 195 Query: 180 IGSHTVAVMLEPVQG-EGGVIPATKEFMQGLRRLTKEKNLLLIVDEVQAGMGRTGQLFAY 238 +A + E V G G +P ++ + +R + + +LLI+DEV +GMGRTG LFA Sbjct: 196 GADQVIAFVAETVVGATAGAVPPVGDYFRKIRAVCDKYGVLLILDEVMSGMGRTGYLFAC 255 Query: 239 QHSGIEPDIMTLAKGIGGGV-PLAALLAREEI--ACFEAG---EQGGTYNGNPLMTAVGV 292 + G+ PDI+ +AKG+G G P+ A++ + I A + G TY G+ A V Sbjct: 256 EEDGVVPDIVVIAKGLGAGYQPIGAMICSDHIYDAVLRGSGFFQHGHTYIGHATACAAAV 315 Query: 293 AVIKELLKPGFMESVRERGQYLRQRSLEISEKYGFEGE----RGEGLLRALQLGRD---- 344 AV K + + +E+VR+RG+ LR E+ + +G + RG GL ++L + Sbjct: 316 AVQKTIQEERLLENVRQRGEQLRS---ELRQAFGDQAHVGDIRGRGLFVGVELVAERSSK 372 Query: 345 --IGPQIVEAARN----------LEPVGLLLNSPRPNLLRFMPALNVTKEEIDQMFSMLE 392 + P + AR + P+G ++ + + P + +I ++ L Sbjct: 373 LPLSPDLRTHARVKAEAMKRGLLVYPMGGTIDGKNGDHILLAPPFIASSNDISEIVQRLS 432 Query: 393 DVLA 396 D +A Sbjct: 433 DAVA 436 >gi|300313229|ref|YP_003777321.1| 4-aminobutyrate aminotransferase [Herbaspirillum seropedicae SmR1] Length = 456 Score = 140 bits (354), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 119/412 (28%), Positives = 190/412 (46%), Gaps = 65/412 (15%) Query: 17 RPELVFTEGHGMWLTDHNGKRYLDYLQGWAVNTLGHSPQCIADALNAQSKKLINPSPAFY 76 R + + G+ D +G ++D G V+++G + +A+ QS+ L++ S Sbjct: 46 RMPIAMDQAFGVTFKDPDGNTFIDLSAGVGVSSVGRCNPRVVEAIRKQSESLMH-SMEVN 104 Query: 77 NAPSMELA----ELLTANSVFDRV-FFANSGAEANEGAIKLARKWGKKNPAADGSSRFEI 131 ++ ELA E++ D + FF G++A E A+K A++ + R +I Sbjct: 105 SSKRTELAAKISEIMPDGLRGDCITFFTQGGSDALEAAVKFAKRV---------TGRHQI 155 Query: 132 ITFKHSFHGRTLATMSASGKDGWDTMFAPQVPG-----FPKAIL----NDLASVEALIGS 182 I F +HG A+ + + + F P + G +P A S E + G Sbjct: 156 IAFHGGYHGIWNASNALTTGTAYRKGFGPFMGGVIHAPYPYAYRFPFDTSHKSAEQIAGE 215 Query: 183 HT--------------VAVMLEPVQGEGGVIPATKEFMQGLRRLTKEKNLLLIVDEVQAG 228 + AV++EPVQGEGG +P + EF+Q LR+ LLIVDEVQAG Sbjct: 216 YVDYLLNTPYTAADDVAAVIVEPVQGEGGYVPPSPEFLQILRKACDRSGALLIVDEVQAG 275 Query: 229 MGRTGQLFAYQHSGIEPDIMTLAKGIGGGVPLAALLAREEIAC-FEAGEQGGTYNGNPLM 287 GRTG+++A +HSG++PD++T KGIGG +P+A L+ R ++A G Q T+ N + Sbjct: 276 AGRTGKMWAVEHSGVKPDMLTFGKGIGGDMPMAGLVMRSDLAAKIPDGSQPNTFAANSIS 335 Query: 288 TAVGVAVIKELLKPGFMESVRERGQYLRQRSLEISEKYGFEGE-RGEGLLRALQLGRDIG 346 AV + I L P R L + S + GE RG GL+ IG Sbjct: 336 AAVALTNISILQDPRLDLVNRAHTLGLEAQERIRSFNSPWVGEVRGRGLM--------IG 387 Query: 347 PQIVEAARNLEPVG----------------LLLNSPR-PNLLRFMPALNVTK 381 ++VE EP+ L++ R N++R MP+L + + Sbjct: 388 IELVENRETREPLSREKLGKLMDYVVGHGVLMIPCGRYTNVMRVMPSLTIPR 439 >gi|300310456|ref|YP_003774548.1| family II aminotransferase [Herbaspirillum seropedicae SmR1] Length = 458 Score = 125 bits (313), Expect = 8e-30, Method: Compositional matrix adjust. Identities = 117/425 (27%), Positives = 201/425 (47%), Gaps = 53/425 (12%) Query: 20 LVFTEGHGMWLTDHNGKRYLDYLQG-WAVNTLGHSPQCIADALNAQSKKLINPSPAFYNA 78 L+ G G +LTDH K+ LD G W VNT G+ + + A Q ++L + F+ A Sbjct: 30 LILKSGQGAYLTDHQDKQLLDAFSGLWCVNT-GYGQESVIRAATEQMQRLPYATGYFHFA 88 Query: 79 --PSMELAELLT--ANSVFDRVFFANSGAEANEGAIKLARKWGKKNPAADGSSRFEIITF 134 P++ LA+ L + + V+F G++A + AI+ + + G F I+ Sbjct: 89 SEPAILLAKKLVDISPASLQHVYFTLGGSDAVDAAIRYITHY-HHSIGKPGKQHF--ISL 145 Query: 135 KHSFHGRTLATMSASGKDGWDTMFAPQVPG-------FP--------KAILNDLASVEAL 179 + +HG + + + F +P +P +AI+ ASV+AL Sbjct: 146 QRGYHGSSSVGAGLTALPNFHYHFNLPLPTQHYLPSPYPYRSELQGDEAIIA--ASVQAL 203 Query: 180 ------IGSHTVAVML-EPVQGEGGVIPATKEFMQGLRRLTKEKNLLLIVDEVQAGMGRT 232 +G+ VA EP+QG GGV+ K +++ ++ E ++L +VDEV G GRT Sbjct: 204 RDKVAELGADNVAAFFCEPIQGSGGVVVPPKGWLKAMQLACNELDILFVVDEVITGFGRT 263 Query: 233 GQLFAYQHSGIEPDIMTLAKGIGGG-VPLAALLAREEIACFEAG--------EQGGTYNG 283 G +FA ++PD+MT+AKG+ G P+ A++ + + A G TY+ Sbjct: 264 GPMFACLDEDVQPDLMTMAKGLTAGYAPMGAVMMSDRVYNGIADGAPMSTVVGHGQTYSA 323 Query: 284 NPLMTAVGVAVIKELLKPGFMESVRERGQYLRQRSLEISEKYGFEGE-RGEGLLRALQLG 342 +P+ AV + V++ + G +++ R R + + L + G+ R GLL AL+L Sbjct: 324 HPVSAAVALEVLRLYEEGGLLDNGR-RLEPVFAGGLRALLAHPLVGDARSRGLLGALELV 382 Query: 343 RD------IGPQIVEAARNLEPV---GLLLNSPRPNLLRFMPALNVTKEEIDQMFSMLED 393 D P++ R + GL+ + N+L F PAL T+E+ MF+ L+ Sbjct: 383 ADKQTKARFAPELKLHDRIFQAAYRHGLVFRAFGDNILGFAPALCYTQEDFALMFARLKA 442 Query: 394 VLAKI 398 +L ++ Sbjct: 443 ILDEV 447 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.135 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,162,353 Number of extensions: 48378 Number of successful extensions: 172 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 152 Number of HSP's successfully gapped: 10 Length of query: 400 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 313 Effective length of database: 1,207,925 Effective search space: 378080525 Effective search space used: 378080525 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)