BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0179.p (300 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosp... 612 e-176 gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosp... 388 e-109 gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosp... 201 4e-53 gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosp... 131 8e-32 gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase ... 100 2e-22 >gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 578 Score = 612 bits (1578), Expect = e-176, Method: Compositional matrix adjust. Identities = 295/300 (98%), Positives = 299/300 (99%) Query: 1 VIHLLAIAGRIGIQLDLKDWDDIGQQLPCLLDLQPSGKFLMEDFYYAGGLPAVIRQLESV 60 VIHLLAIAGRIGI+LDLKDWDDIGQQLPCLLDLQPSGKFLMEDFYYAGGLPAVIRQLESV Sbjct: 279 VIHLLAIAGRIGIKLDLKDWDDIGQQLPCLLDLQPSGKFLMEDFYYAGGLPAVIRQLESV 338 Query: 61 IDKTALTANGKTLWENCQDAPNWNPEVIRSFDQPFKEAAGIAILKGNLAPDGAVIKPSAA 120 IDKTALTANGKTLWENCQDAPNWN EVIRSFD+PFKEAAGIAILKGNLAPDGAVIKPSAA Sbjct: 339 IDKTALTANGKTLWENCQDAPNWNEEVIRSFDKPFKEAAGIAILKGNLAPDGAVIKPSAA 398 Query: 121 TPALLKHRGRAVVFENSDDLHKRIDDENLDVDETCVLVLKNCGPKGYPGMAEAGNMPLPP 180 TPALLKHRGRAVVFENSDDLHKRIDDENLDVDETCVLVLKNCGPKGYPGMAEAGNMPLPP Sbjct: 399 TPALLKHRGRAVVFENSDDLHKRIDDENLDVDETCVLVLKNCGPKGYPGMAEAGNMPLPP 458 Query: 181 KVLRKGITDMVRVSDARMSGTAYGTVVLHVSPEAAAGGPLALVQNGDFIELDVEARKLHL 240 K+LRKGITDMVRVSDARMSGTAYGTVVLHVSPEAAAGGPLA+VQNGDFIELDVEARKLHL Sbjct: 459 KILRKGITDMVRVSDARMSGTAYGTVVLHVSPEAAAGGPLAVVQNGDFIELDVEARKLHL 518 Query: 241 DVSDEELARRRAQWQKPELPPQMQRGWVKLYVDHVQQANQGADLDFLVGKSGPYVPKDNH 300 DVSDEELARRRAQWQKPELPPQMQRGWVKLYVDHVQQANQGADLDFLVGKSGPYVPKDNH Sbjct: 519 DVSDEELARRRAQWQKPELPPQMQRGWVKLYVDHVQQANQGADLDFLVGKSGPYVPKDNH 578 >gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 585 Score = 388 bits (996), Expect = e-109, Method: Compositional matrix adjust. Identities = 192/305 (62%), Positives = 236/305 (77%), Gaps = 5/305 (1%) Query: 1 VIHLLAIAGRIGIQLDLKDWDDIGQQLPCLLDLQPSGKFLMEDFYYAGGLPAVIRQL--- 57 VIHL AIAGRIG+ L+L+DW IG+ P ++DLQPSG++LME+FYYAGGLPAV+R+L Sbjct: 281 VIHLKAIAGRIGVDLELEDWTRIGRGTPTIVDLQPSGRYLMEEFYYAGGLPAVLRRLGEA 340 Query: 58 ESVIDKTALTANGKTLWENCQDAPNWNPEVIRSFDQPFKEAAGIAILKGNLAPDGAVIKP 117 + + K ALT NG+T+W+N +DAP +N EV+R +P E GI IL+GNLAP GAV+KP Sbjct: 341 DLLPHKDALTVNGQTMWDNVKDAPIYNDEVVRPLAKPLIEDGGICILRGNLAPRGAVLKP 400 Query: 118 SAATPALLKHRGRAVVFENSDDLHKRIDDENLDVDETCVLVLKNCGPKGYPGMAEAGNMP 177 SAATP L+KHRGRAVVFE+ + +RI+D +LDVD +CVLV+KN GPKGYPGMAE GNM Sbjct: 401 SAATPELMKHRGRAVVFEDFNHYKERINDPDLDVDASCVLVMKNVGPKGYPGMAEVGNMG 460 Query: 178 LPPKVLRKGITDMVRVSDARMSGTAYGTVVLHVSPEAAAGGPLALVQNGDFIELDVEARK 237 LPPKVL G+ DMVR+SDARMSGTAYGTV+LHV+PEAAAGGPL +VQ+GDFIELD A K Sbjct: 461 LPPKVLATGVKDMVRISDARMSGTAYGTVILHVAPEAAAGGPLGIVQDGDFIELDAYAGK 520 Query: 238 LHLDVSDEELARRRAQWQK--PELPPQMQRGWVKLYVDHVQQANQGADLDFLVGKSGPYV 295 L LD+S+EE+ RR K E P+M G+ LYVD V QA++G D DFLVG G V Sbjct: 521 LQLDISEEEMKRRLEARAKVLAERKPEMVGGYQSLYVDRVLQADEGCDFDFLVGCRGAAV 580 Query: 296 PKDNH 300 PK +H Sbjct: 581 PKHSH 585 >gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 201 bits (512), Expect = 4e-53, Method: Compositional matrix adjust. Identities = 114/313 (36%), Positives = 183/313 (58%), Gaps = 18/313 (5%) Query: 3 HLLAIAGRIGIQLDLKDWDDIGQQLPCLLDLQPSGKFLMEDFYYAGGLPAVIRQLESV-- 60 H++A+A G++L +DW G +P LL++QP+GK+L E F+ AGG+PA++ +L+ Sbjct: 285 HIMAMARHAGVELQSEDWMKYGYDVPLLLNMQPAGKYLGERFHRAGGVPAIMWELQQAGK 344 Query: 61 IDKTALTANGKTLWENCQDAPNWNPEVIRSFDQPFKEAAGIAILKGNLAPDGAVIKPSAA 120 + +TA GKT+ EN Q + + E+I F P +E AG +LKGNL D A++K S Sbjct: 345 LRAERITATGKTMAENLQGRASNDREMIYPFAAPLRERAGFLVLKGNLF-DFAIMKTSVI 403 Query: 121 TPALLKH-----------RGRAVVFENSDDLHKRIDDENLDVDETCVLVLKNCGPKGYPG 169 + + RAVVF+ SDD H RI+D L +DE +L ++ GP G+PG Sbjct: 404 SETFRERYLSTPGQENIFECRAVVFDGSDDYHARINDPALKIDENTLLAIRGAGPVGWPG 463 Query: 170 MAEAGNMPLPPKVLRKGITDMVRVSDARMSGTAYGTVVLHVSPEAAAGGPLALVQNGDFI 229 AE NM P ++++G++ + + D R SGT+ +L+ SPE+A GG LA +++GD + Sbjct: 464 SAEVVNMQPPDALIKRGVSTLPTLGDGRQSGTSDSPSILNASPESAVGGGLAYLRDGDRV 523 Query: 230 ELDVEARKLHLDVSDEELARRRAQWQKPELPPQMQRGWVKLYVDHVQQANQGADLDFLVG 289 +D+ + ++ VS+EELARR+++ P +PP Q W ++Y V Q GA ++ + Sbjct: 524 RIDLNTGECNMLVSEEELARRKSE-GIPPVPPS-QTPWQEIYRSTVGQLETGACMELALK 581 Query: 290 KSG--PYVPKDNH 300 G +P+ NH Sbjct: 582 YQGVAQTLPRHNH 594 >gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 620 Score = 131 bits (329), Expect = 8e-32, Method: Compositional matrix adjust. Identities = 99/339 (29%), Positives = 163/339 (48%), Gaps = 62/339 (18%) Query: 1 VIHLLAIAGRIGIQLDLKDWDDIGQQLPCLLDLQP-SGKFLMEDFYYAGGLPAVIRQLE- 58 V+HLLA A ++ + D D I +++PCL + P + K+ +ED + AGG+ +++ +L Sbjct: 286 VLHLLAAAHEAKVEFTMADIDRISRRVPCLCKVAPMTNKYHIEDVHRAGGIISILGELAR 345 Query: 59 -SVIDKTALTANGKTLWENCQD-----------------APNWNPE-------------- 86 ++D + T + KTL E + AP P Sbjct: 346 AGLLDTSRPTVHSKTLGEAIEKFDIRVSSDPAVHKLFRAAPGGVPTQVAFSQEERFEAND 405 Query: 87 ------VIRSFDQPFKEAAGIAILKGNLAPDGAVIKPSAATPALLKHRGRAVVFENSDDL 140 IR + + + G+A+L GN+A G ++K + ++LK G A VFE+ D Sbjct: 406 LDRANGCIRDREHAYSQDGGLAVLYGNIAEKGCIVKTAGVDESILKFSGTARVFESQDAA 465 Query: 141 HKRIDDENLDVDETCVLVLKNCGPKGYPGMAEAGNMPLPPKVLR-KGITDMVRV-SDARM 198 + I + + + V++++ GPKG PGM E M P ++ KG+ + +D R Sbjct: 466 VEGILGDKVKAGD--VVIVRYEGPKGGPGMQE---MLYPTSYIKSKGLGKACALFTDGRF 520 Query: 199 SGTAYGTVVLHVSPEAAAGGPLALVQNGDFIELDVEARKLHLDVSDEELARRR------- 251 SG + G V+ H SPEAA GG + LV+ GD I++D+ R ++L +S ++LA+RR Sbjct: 521 SGGSSGLVIGHASPEAAEGGAIGLVEEGDMIDIDIPNRTINLRISSDDLAKRRTAMEARG 580 Query: 252 -AQWQKPELPPQMQR---GWVKLYVDHVQQANQGADLDF 286 A WQ P QR ++ Y A++GA D Sbjct: 581 DAAWQ----PGNRQRVVSQALQAYAALTTSADRGAVRDL 615 >gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 623 Score = 100 bits (248), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 86/275 (31%), Positives = 127/275 (46%), Gaps = 31/275 (11%) Query: 1 VIHLLAIAGRIGIQLDLKDWDDIGQQLPCLLDLQPSGKFLMEDFYYAGGLPAVIRQL--E 58 +HL+AIA GI +D D+D + +P + + P+G + F+ AGG V+R+L Sbjct: 306 TLHLVAIAKAAGIVIDWDDFDKLSAVVPLITKIYPNGTADVNHFHAAGGTGFVLRELIDA 365 Query: 59 SVIDKTALTANGKTLWENCQ-------------------DAPNWNPE--VIRSFDQPFKE 97 ++ T G+ L ++C+ DAP + + V+ + +PF Sbjct: 366 GLMHDDVTTILGQGLRQHCKEPMLAAEGENGRPGIVTWHDAPAQSGDEGVLGTVAKPFAA 425 Query: 98 AAGIAILKGNLAPDGAVIKPSAATPALLKHRGRAVVFENSDDLHKRIDDENLDVDETCVL 157 G+ +L+GNL AVIK SA P AVVF + + LD D V+ Sbjct: 426 DGGLKLLQGNLGR--AVIKISAVKPEHRVVEAPAVVFHSQEAFMAAFKAGQLDRDFVAVI 483 Query: 158 VLKNCGPKGYPGMAEAGNMPLPPKVLRKGITDMVRVSDARMSGTAYGTV--VLHVSPEAA 215 + GP+ GM E + +L+ + V+D RMSG A G V +HV+PE Sbjct: 484 TFQ--GPRAN-GMPELHALTPALGILQDKGRHVALVTDGRMSG-ASGKVPAAIHVTPEVL 539 Query: 216 AGGPLALVQNGDFIELDVEARKLHLDVSDEELARR 250 GGPL V++GD I LD EA L V E A R Sbjct: 540 GGGPLGRVRDGDIIRLDAEAGTLEAKVDATEWAAR 574 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.138 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,068,411 Number of extensions: 46888 Number of successful extensions: 141 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 132 Number of HSP's successfully gapped: 5 Length of query: 300 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 215 Effective length of database: 1,217,395 Effective search space: 261739925 Effective search space used: 261739925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)