BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0172.p (445 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312638|ref|YP_003776730.1| cytochrome c precursor protein ... 876 0.0 gi|300313762|ref|YP_003777854.1| cytochrome c precursor protein ... 433 e-122 gi|300313847|ref|YP_003777939.1| membrane-bound D-gluconate 2-de... 389 e-109 gi|300310719|ref|YP_003774811.1| cytochrome c protein [Herbaspir... 293 2e-80 gi|300313053|ref|YP_003777145.1| gluconate 2-dehydrogenase [Herb... 279 3e-76 >gi|300312638|ref|YP_003776730.1| cytochrome c precursor protein [Herbaspirillum seropedicae SmR1] Length = 441 Score = 876 bits (2263), Expect = 0.0, Method: Compositional matrix adjust. Identities = 421/441 (95%), Positives = 429/441 (97%) Query: 5 MIKHYFIAACAAVMSLSAFTAAAQSNAAAPSADQQLVQRGEYLAKAGDCVACHTAKGGKP 64 MIKHYFIAACAAVMSLSA TAAAQSN AAPSADQQLVQRGEYLAKAGDCVACHTAKGGKP Sbjct: 1 MIKHYFIAACAAVMSLSALTAAAQSNPAAPSADQQLVQRGEYLAKAGDCVACHTAKGGKP 60 Query: 65 FAGGLPIATPIGTVYSSNITPDKEHGIGNYSEEDFDRALRHGIRKDGASLYPAMPYPSYA 124 FAGGL IATPIGTVYSSNITPDKE+GIGNYSEEDFDRALRHGIRKDGASLYPAMPYPSYA Sbjct: 61 FAGGLAIATPIGTVYSSNITPDKENGIGNYSEEDFDRALRHGIRKDGASLYPAMPYPSYA 120 Query: 125 KVKPADVKALYAYFMQGVQADPAPNRGVDITWPLSMRWPLSVWRKVFAPAVTADGPEDNS 184 KVKPADVKALYAYFM GVQADPAPNRGVDITWPLSMRWPLS+WRKVFAPAV DGPEDNS Sbjct: 121 KVKPADVKALYAYFMHGVQADPAPNRGVDITWPLSMRWPLSIWRKVFAPAVAVDGPEDNS 180 Query: 185 PLVRGQYLVEGLGHCGACHTPRGVGMQEKALSNDSSQFLSGGVIDGYLANNLRGDARDGL 244 PLVRGQYLVEGLGHCGACHTPRGVGMQEKALSNDSSQFLSGGVIDGYLANNLRGD RDGL Sbjct: 181 PLVRGQYLVEGLGHCGACHTPRGVGMQEKALSNDSSQFLSGGVIDGYLANNLRGDGRDGL 240 Query: 245 GNWSEADIVAFLKTGRNSHSAAFGGMADVVANSTQYMTGEDLSAMAKYLKSLKAVKEGTA 304 GNWSEADIVAFLKTGRNSHSAAFGGMADVVANSTQYMT EDLSAMAKYLKSLK VK+GT Sbjct: 241 GNWSEADIVAFLKTGRNSHSAAFGGMADVVANSTQYMTEEDLSAMAKYLKSLKPVKDGTP 300 Query: 305 ALAYDDKTHQALRKGSDQSPGAMAFLNNCAACHRSSGKGYEETFPTLALSPTVNAENPAS 364 ALAYDDKTHQALRKGSDQSPGAMAFLNNCAACHRSSGKGY+ETFP+LALSPTVNAENPAS Sbjct: 301 ALAYDDKTHQALRKGSDQSPGAMAFLNNCAACHRSSGKGYDETFPSLALSPTVNAENPAS 360 Query: 365 LIRIVLEGAEMPWTHKAPTQFAMPGFASRLSDQEVADVVTFIRSSWGNQASSVNASDVAK 424 LIRIVLEGAEMPWTHKAPTQFAMP F SRLSDQEVA+VVTFIRSSWGNQASSV+ASDVAK Sbjct: 361 LIRIVLEGAEMPWTHKAPTQFAMPAFGSRLSDQEVAEVVTFIRSSWGNQASSVSASDVAK 420 Query: 425 VRKQLPEKKTVEAGSAPLAKQ 445 VRKQLPEKKTVEAGSAP+AKQ Sbjct: 421 VRKQLPEKKTVEAGSAPVAKQ 441 >gi|300313762|ref|YP_003777854.1| cytochrome c precursor protein [Herbaspirillum seropedicae SmR1] Length = 482 Score = 433 bits (1114), Expect = e-122, Method: Compositional matrix adjust. Identities = 217/413 (52%), Positives = 284/413 (68%), Gaps = 7/413 (1%) Query: 21 SAFTAAAQSNAA--APSADQQLVQRGEYLAKAGDCVACHTAKGGKPFAGGLPIATPIGTV 78 +A TA AQ AA + SA+ Q + RG YLA+AGDCVACHT+KGG PFAGGL +A+PIG + Sbjct: 61 AADTALAQRIAAQGSASAEDQQILRGAYLARAGDCVACHTSKGGAPFAGGLALASPIGAI 120 Query: 79 YSSNITPDKEHGIGNYSEEDFDRALRHGIRKDGASLYPAMPYPSYAKVKPADVKALYAYF 138 YS+NITPDK+HGIG++S EDF R +R G+ K G ++YPAMPYPSY+++ D++ALYAYF Sbjct: 121 YSTNITPDKQHGIGDWSYEDFARLMRTGVTKAGYTVYPAMPYPSYSRLTDEDMQALYAYF 180 Query: 139 MQGVQADPAPNRGVDITWPLSMRWPLSVWRKVFAPAVTADGPEDNS--PLVRGQYLVEGL 196 + V NR DI WPLSMRWPL++WRKVFAP P S L RG YLVEGL Sbjct: 181 SKAVPPSAQENRANDIPWPLSMRWPLALWRKVFAPTPAPYTPAAGSDQELARGAYLVEGL 240 Query: 197 GHCGACHTPRGVGMQEKALSNDSSQ-FLSGG-VIDGYLANNLRGDARDGLGNWSEADIVA 254 GHCG+CH+PR V MQEKAL D + FLSGG V+DG+ +LR + G+ WS+AD+V Sbjct: 241 GHCGSCHSPRAVTMQEKALKEDGGRLFLSGGQVVDGWSVPSLRNEHGGGIAGWSQADLVE 300 Query: 255 FLKTGRNSHSAAFGGMADVVANSTQYMTGEDLSAMAKYLKSLKAVKEGTAALAYDDKTHQ 314 FL+TGRN ++A+FG M DV+ +S QYM+ DL+AMA+YL SL ++ A YD T Q Sbjct: 301 FLRTGRNQYTASFGAMNDVIEDSMQYMSDADLNAMARYLLSLPP-RQQAAPYRYDSATAQ 359 Query: 315 ALRKGSDQSPGAMAFLNNCAACHRSSGKGYEETFPTLALSPTVNAENPASLIRIVLEGAE 374 A G PGA +L+ CAACHRS+G GY + FP LA +P + + S IRI+L+G Sbjct: 360 AAYDGRPDGPGARIYLDRCAACHRSNGTGYGKAFPALAGNPVLQTSDATSAIRIILQGGR 419 Query: 375 MPWTHKAPTQFAMPGFASRLSDQEVADVVTFIRSSWGNQASSVNASDVAKVRK 427 P T A M +A L DQ+VA+V ++I+++WGN+ + A++VAKVRK Sbjct: 420 QPSTASATAGLVMAPYAQLLDDQQVAEVTSYIQTAWGNRGGTTTAAEVAKVRK 472 >gi|300313847|ref|YP_003777939.1| membrane-bound D-gluconate 2-dehydrogenase cytochrome c subunit [Herbaspirillum seropedicae SmR1] Length = 423 Score = 389 bits (998), Expect = e-109, Method: Compositional matrix adjust. Identities = 197/406 (48%), Positives = 261/406 (64%), Gaps = 13/406 (3%) Query: 33 APSADQQLVQRGEYLAKAGDCVACHTAKGGKPFAGGLPIATPIGTVYSSNITPDKEHGIG 92 A +ADQ LV RG+YLA+AGDC+ACH+A G ++GGL I + G +YS+NITPDKEHGIG Sbjct: 24 AQAADQDLVARGQYLARAGDCMACHSAAGKPAYSGGLAIDSGHGIIYSTNITPDKEHGIG 83 Query: 93 NYSEEDFDRALRHGIRKDGASLYPAMPYPSYAKVKPADVKALYAYFMQGVQADPAPNRGV 152 NYSE F A+RHG+R DG LYPAMPYPSYAKV D+ ALY YFMQGVQ + Sbjct: 84 NYSEAQFSAAVRHGVRADGTQLYPAMPYPSYAKVSDEDIHALYTYFMQGVQPVASTPPAS 143 Query: 153 DITWPLSMRWPLSVWRKVFA---PAVTADGPEDNSPLVRGQYLVEGLGHCGACHTPRGVG 209 +++P ++RW + +W FA P DG ++ + RG YLVEGLGHCG+CHTPRGV Sbjct: 144 SMSFPFNIRWGMKLWNVFFANDKPFREQDGW--SAEIKRGAYLVEGLGHCGSCHTPRGVA 201 Query: 210 MQEKALSNDSSQFLSGGVIDGYLANNLRGDARDGLGNWSEADIVAFLKTGRNSHSAAFGG 269 M EKA + +QFLSGG ++G+ +LR G+ +WS DIV +L+TGRN ++ G Sbjct: 202 MNEKASDSSQAQFLSGGDLNGWAVPSLR-----GMPHWSAQDIVDYLQTGRNKTASVAGE 256 Query: 270 MADVVANSTQYMTGEDLSAMAKYLKSLKAVKEGTAALAYD---DKTHQALRKGSDQSPGA 326 M+ VV +ST ++ EDL AMA YLK+L V + D T L D + G Sbjct: 257 MSLVVEHSTSHLKREDLQAMAAYLKTLSPVASSGSGRVIPQGVDATVSKLTAAKDLTLGE 316 Query: 327 MAFLNNCAACHRSSGKGYEETFPTLALSPTVNAENPASLIRIVLEGAEMPWTHKAPTQFA 386 +L+NCAACH +G+G FP L + VNAENP++L+ ++L GA P T KAP+ Sbjct: 317 RLYLDNCAACHFVTGRGAPGIFPVLDGATVVNAENPSALLHVILAGARTPSTEKAPSILV 376 Query: 387 MPGFASRLSDQEVADVVTFIRSSWGNQASSVNASDVAKVRKQLPEK 432 MPGFA RLSD+E A + TF+R WGN A V+ +V K+R + ++ Sbjct: 377 MPGFAHRLSDEEAAALATFVRQGWGNHAGKVSEREVGKLRAGMDKQ 422 >gi|300310719|ref|YP_003774811.1| cytochrome c protein [Herbaspirillum seropedicae SmR1] Length = 431 Score = 293 bits (749), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 173/398 (43%), Positives = 232/398 (58%), Gaps = 16/398 (4%) Query: 33 APSADQQLVQRGEYLAKAGDCVACHTAKGGKPFAGGLPIATPIGTVYSSNITPDKEHGIG 92 AP +QL Q G+YLA+AG+C+ACHTA+GG P+AGG IA+P GT+Y+SNITPDK GIG Sbjct: 41 APDPTRQLAQ-GQYLARAGNCMACHTARGGAPYAGGRAIASPFGTLYTSNITPDKTTGIG 99 Query: 93 NYSEEDFDRALRHGIRKDGASLYPAMPYPSYAKVKPADVKALYAYFMQGVQADPAPNRGV 152 +S +DF RAL HG K+G LYPA PY SY ++ AD AL+AY +Q + N+ Sbjct: 100 QWSADDFWRALHHGRGKEGQFLYPAFPYTSYTRLSRADSDALFAY-LQSLPPVARANQAH 158 Query: 153 DITWPLSMRWPLSVWRKV-FAPAVTADGPEDNSPLVRGQYLVEGLGHCGACHTPRG-VGM 210 + +P R L +WR + F P P ++ RG YLV+G GHC ACH+ R +G Sbjct: 159 TLRFPYDQRMLLGLWRALYFTPQSYQPDPRQDAQWNRGAYLVQGAGHCSACHSARNRLGA 218 Query: 211 QEKALSNDSSQFLSGGVIDG--YLANNLRGDARDGLGNWSEADIVAFLKTGRNSHSAAFG 268 + L+ L+GGVI + A L G A DGL +WS ADI A LKTG + H+ G Sbjct: 219 SIEPLA------LAGGVIPALQWYAPPLHGGA-DGLQDWSTADIAALLKTGVSPHAVTLG 271 Query: 269 GMADVVANSTQYMTGEDLSAMAKYLKSLKAVKEGTAALAYDDKTHQALRKGSDQSPGAMA 328 M+++V S QY+ D+SAMA YLKSL A AA + +A R G Sbjct: 272 PMSEIVGRSLQYLNDADVSAMAVYLKSLPASAADDAARPASVEPAEAERI---MKQGKQL 328 Query: 329 FLNNCAACHRSSGKGYEETFPTLALSPTVNAENPASLIRIVLEGAEMPWTHKAPTQFAMP 388 + C CH G+G +P LA + + +PA+ +R VL G P T P F MP Sbjct: 329 YGTLCVDCHGGKGQGSAPDYPPLAGNRAIAGPHPANAVRAVLNGGFPPSTAGNPYPFGMP 388 Query: 389 GFASRLSDQEVADVVTFIRSSWGNQASSVNASDVAKVR 426 F +LSDQEVA VV+++R+SWGNQ V+A++V + R Sbjct: 389 PFGPQLSDQEVAAVVSYVRNSWGNQGGLVSAAEVNRYR 426 >gi|300313053|ref|YP_003777145.1| gluconate 2-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 499 Score = 279 bits (714), Expect = 3e-76, Method: Compositional matrix adjust. Identities = 166/414 (40%), Positives = 238/414 (57%), Gaps = 29/414 (7%) Query: 40 LVQRGEYLAKAGDCVACHTAKGGKPFAGGLPIATPIGTVYSSNITPDKEHGIGNYSEEDF 99 L++RG YLA A DC+ACHTA GKP AGGL IA+P+G + ++NITP K HGIGNY+EE F Sbjct: 52 LIERGHYLAIASDCIACHTAPRGKPMAGGLAIASPVGEIIATNITPSKTHGIGNYTEEQF 111 Query: 100 DRALRHGIRKDGASLYPAMPYPSYAKVKPADVKALYAYFMQGVQADPAPNRGVDITWPLS 159 ALR G+R DGA+LYPAMPY +YA + ADV+ALYAYFM+GV R D+ +P++ Sbjct: 112 AAALRRGVRADGANLYPAMPYTAYAALSDADVQALYAYFMKGVAPVDVATRPTDLPFPMN 171 Query: 160 MRWPLSVWRKVFAPAV-TADGPEDNSPLVRGQYLVEGLGHCGACHTPRGVGMQEKALSND 218 +RW + W +F P+ ++ +RG+YL EG HC CHTPRG MQEK Sbjct: 172 LRWSMKAWNALFLKEQPLPPQPQRSAQWLRGRYLAEGPAHCSTCHTPRGWLMQEK----- 226 Query: 219 SSQFLSGGVIDGYLANNLRGDARDGLGNWSEADIVAFLKTGR-NSHSAAFGGMADVVANS 277 L+G + + A N+ G+G+WS+ D+VA+L+TGR + A G MA+ + +S Sbjct: 227 PDLQLAGAQVGPWYAPNITSHPSAGIGSWSQDDLVAYLRTGRLEGRAQAAGSMAEAITHS 286 Query: 278 TQYMTGEDLSAMAKYLKSLKAVKEGTAALA---YDD-KTHQAL-----RKGSDQSP---- 324 +T EDL A+A Y+K A+ G A + +D K AL R S +SP Sbjct: 287 FSRLTEEDLRAIAVYIKDRPAIATGDAQGSGSRFDQGKAGNALAQFRGRTLSAESPEEIR 346 Query: 325 GAMAFLNNCAACHRSSGKGYEE-TFPTLALSPTVNAENPASLIRIVLEGAEMPWTHKAPT 383 GA F +CA+CH +G+G + +P+L + N +LI +L G + T + Sbjct: 347 GARIFSASCASCHGYNGQGSRDGYYPSLFRNSATAGVNANNLIATILYGVDRE-TEQGGH 405 Query: 384 QFAMPGFA------SRLSDQEVADVVTFIRSSWGNQASSVNASDVAKVRKQLPE 431 F MP F ++LS+ +VA + ++ +G A++V A DV +R+ P Sbjct: 406 VF-MPPFGDQPNALNKLSNADVAALSNYLLKYYGKPAATVKAEDVEVIRQGGPR 458 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.130 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,402,079 Number of extensions: 60247 Number of successful extensions: 231 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 185 Number of HSP's successfully gapped: 8 Length of query: 445 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 357 Effective length of database: 1,203,190 Effective search space: 429538830 Effective search space used: 429538830 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)