BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0165.p (417 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310279|ref|YP_003774371.1| HD-GYP domain-containing protei... 629 0.0 gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent p... 466 e-132 gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protei... 410 e-115 gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protei... 327 1e-90 gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protei... 325 5e-90 >gi|300310279|ref|YP_003774371.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 408 Score = 629 bits (1622), Expect = 0.0, Method: Compositional matrix adjust. Identities = 295/409 (72%), Positives = 344/409 (84%), Gaps = 6/409 (1%) Query: 1 MTQLKKISVNDLAVGMYFSGFDGPWIDNPFWRSKFLIEQAADVSRARSAGFEFCWIDVSL 60 MT+LKKI V L+VGMYFSGFDG WID PFWR+ FL+ +A++++RAR+AG+ +CWID Sbjct: 1 MTRLKKIEVGQLSVGMYFSGFDGAWIDTPFWRTSFLVRKASEIARARAAGYTYCWIDTDQ 60 Query: 61 GRDVIPGSAIDAMDVAAEPRVELVPLTPEDEYRNELSEALRLYESAKVSTQQMFRQARLG 120 G D + G+ D + EP R+EL +ALRLYE+AK +TQQMF+QARLG Sbjct: 61 GID-LAGAEADRLPETEEPEA-----AGSSSSRSELDDALRLYENAKQTTQQMFKQARLG 114 Query: 121 KTLDVAQCQDLVEEIAASVTRNSNALLSIIRLKRADEYTYLHSIAVCTMMVALARTLGMD 180 KTLDV C+ LVEEIA SV R+SNALLSIIRLKR DEYTYLHSIAVCT+MVALARTLGMD Sbjct: 115 KTLDVENCKSLVEEIATSVARDSNALLSIIRLKRVDEYTYLHSIAVCTLMVALARTLGMD 174 Query: 181 NTACKEAGLAGYLHDVGKAFIPLDVLNKPGKLTDQEFRIIQRHTTLGYEYLSGFAGVPDY 240 + ACKEAGLAGYLHDVGKAFIPL++LNKP +L++QE++IIQRH LGY+YL GF+GVPDY Sbjct: 175 DIACKEAGLAGYLHDVGKAFIPLEILNKPDRLSEQEYKIIQRHPALGYDYLKGFSGVPDY 234 Query: 241 VLDVCLHHHEKIDGTGYPGRQQGNQIDLYSRMAAVCDVYDAITSDRTYKSAWDPAESLAR 300 VLDVCLHHHEKIDG+GYP R QGNQI +YSRMAAVCDVYDA+TS+R YKS WDPAESL+R Sbjct: 235 VLDVCLHHHEKIDGSGYPSRHQGNQISVYSRMAAVCDVYDAVTSERPYKSGWDPAESLSR 294 Query: 301 MASWSGHFDRPIFLTFVKTLGIYPIGSIVKLRSGRTALVIRQNHKDLTKPVVKAFTTALS 360 MASW GHFDRPIFL FVK LGIYPIGSIVKL+SGRTALVIRQNH DLTKPVVKAF T S Sbjct: 295 MASWQGHFDRPIFLAFVKALGIYPIGSIVKLQSGRTALVIRQNHVDLTKPVVKAFVTTPS 354 Query: 361 IESLESEIIDLSSPDQDDVISERSGQDWIRSNGLVLDSLKALVEGNERP 409 IE +ESEIIDLS+P Q D I+ERSGQDW++SNGLV+DSLKA++E ++ P Sbjct: 355 IEVVESEIIDLSAPGQTDAIAERSGQDWLKSNGLVIDSLKAMMEASDLP 403 >gi|300310307|ref|YP_003774399.1| HD-GYP domain metal-dependent phosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 406 Score = 466 bits (1199), Expect = e-132, Method: Compositional matrix adjust. Identities = 227/408 (55%), Positives = 296/408 (72%), Gaps = 12/408 (2%) Query: 4 LKKISVNDLAVGMYFSGFDGPWIDNPFWRSKFLIEQAADVSRARSAGFEFCWIDVSLGRD 63 LKKI V DL +GMYF GF+ W+D+PFW++KFL++ +++ +A ++G +CWID G D Sbjct: 2 LKKIKVEDLVLGMYFCGFEAAWLDHPFWKNKFLLKNDSELRQAHASGIAYCWIDTERGLD 61 Query: 64 VIPGSAIDAMDVAAEPRVELVPLTPEDEYRNE------LSEALRLYESAKVSTQQMFRQA 117 V +A D AA P E P +PE E+ L +A+ L E+AK +T+ MF +A Sbjct: 62 V--AQEEEAADSAA-PLQETEP-SPEPEHAAAPPIDAVLLDAMALRETAKKATKAMFSEA 117 Query: 118 RLGKTLDVAQCQDLVEEIAASVTRNSNALLSIIRLKRADEYTYLHSIAVCTMMVALARTL 177 R+GK LD+ +C +VEEIA SV ++ N LS++RLK ADEYTYLHS+AVC +MVAL RTL Sbjct: 118 RMGKVLDMERCVSVVEEIAGSVIKSPNGALSVLRLKLADEYTYLHSVAVCALMVALGRTL 177 Query: 178 GMDNTACKEAGLAGYLHDVGKAFIPLDVLNKPGKLTDQEFRIIQRHTTLGYEYLSGFAGV 237 G+D TAC+EAGL G+LHDVGKAFIP ++LNKPGKL+D+EF I++H LGY+YLS V Sbjct: 178 GLDETACREAGLGGFLHDVGKAFIPHELLNKPGKLSDEEFSQIKKHPELGYKYLSQDPNV 237 Query: 238 PDYVLDVCLHHHEKIDGTGYPGRQQGNQIDLYSRMAAVCDVYDAITSDRTYKSAWDPAES 297 PDY DVCLHHHEKIDG GYP + I LYSRMAA+CDVYDA+TSDR YK AWDPAE+ Sbjct: 238 PDYARDVCLHHHEKIDGNGYPEGLRAGAITLYSRMAAICDVYDALTSDRPYKRAWDPAEA 297 Query: 298 LARMASWSGHFDRPIFLTFVKTLGIYPIGSIVKLRSGRTALVIRQNHKDLTKPVVKAFTT 357 ++ MASW+GHFDR +FL FVKTLGIYP+GSIVKL SGR+ VIRQN +DLT+PV+K F Sbjct: 298 ISAMASWTGHFDRAMFLAFVKTLGIYPVGSIVKLESGRSGFVIRQNAQDLTRPVLKVFHY 357 Query: 358 ALSIESLESEIIDLSSPDQDDVISERSGQDWIRSNGLVLDSLKALVEG 405 S+ ++EI+DL + + D I R G++W R + LD+++ L G Sbjct: 358 DDSMTVTDTEILDLLANPEADTIVARGGEEWARLSH--LDAMQLLSTG 403 >gi|300313282|ref|YP_003777374.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 410 Score = 410 bits (1053), Expect = e-115, Method: Compositional matrix adjust. Identities = 198/387 (51%), Positives = 265/387 (68%), Gaps = 1/387 (0%) Query: 4 LKKISVNDLAVGMYFSGFDGPWIDNPFWRSKFLIEQAADVSRARSAGFEFCWIDVSLGRD 63 LKKI V DL +GMYF GF+ W+ +PFW+++FL+ + +D+ +AR++G +CWIDVS GRD Sbjct: 2 LKKIKVGDLVLGMYFCGFEASWLAHPFWKTRFLLSRESDLQQARASGIAYCWIDVSRGRD 61 Query: 64 VIPGSAIDAMDVAAEPRVELVPLTPEDEYRNELSEALRLYESAKVSTQQMFRQARLGKTL 123 V + A AA + T + S A+ L E A+ T+QMF AR+GK + Sbjct: 62 VAGAAPAKAEAAAAPASPDQSAATHAGADADRAS-AVALREHAREVTRQMFNAARMGKMV 120 Query: 124 DVAQCQDLVEEIAASVTRNSNALLSIIRLKRADEYTYLHSIAVCTMMVALARTLGMDNTA 183 ++ QC LVEEIA SV R+ N LLS++RLK DEYTYLHS++VC +++ L RTLG+ A Sbjct: 121 ELPQCVALVEEIACSVLRDPNGLLSVLRLKLNDEYTYLHSVSVCALVIGLGRTLGLSEAA 180 Query: 184 CKEAGLAGYLHDVGKAFIPLDVLNKPGKLTDQEFRIIQRHTTLGYEYLSGFAGVPDYVLD 243 C+EAGL+GYLHDVGKAFI ++LNKPGKLT +EF +I+RH LGY+YL +P Y D Sbjct: 181 CREAGLSGYLHDVGKAFIDDEILNKPGKLTAEEFALIKRHPELGYQYLIQTPDMPAYASD 240 Query: 244 VCLHHHEKIDGTGYPGRQQGNQIDLYSRMAAVCDVYDAITSDRTYKSAWDPAESLARMAS 303 VC HHHE++DG GYP I LY+RM AVCDVYDA+TS+R YK WDPAE++++MA Sbjct: 241 VCRHHHERLDGRGYPDALPAESITLYARMTAVCDVYDALTSERPYKHGWDPAEAISQMAR 300 Query: 304 WSGHFDRPIFLTFVKTLGIYPIGSIVKLRSGRTALVIRQNHKDLTKPVVKAFTTALSIES 363 W GHFDR + L FVKTLGIYP+GS+V+L SG + +VIRQN DLT+PV+K E+ Sbjct: 301 WEGHFDRRLLLAFVKTLGIYPVGSLVRLESGVSGVVIRQNPADLTRPVLKVLHLQGPHET 360 Query: 364 LESEIIDLSSPDQDDVISERSGQDWIR 390 ++DL + + D I R ++W R Sbjct: 361 TIGGVLDLMAWPRTDTIIGRGDEEWTR 387 >gi|300314021|ref|YP_003778113.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 401 Score = 327 bits (838), Expect = 1e-90, Method: Compositional matrix adjust. Identities = 168/378 (44%), Positives = 228/378 (60%), Gaps = 13/378 (3%) Query: 4 LKKISVNDLAVGMYFSGFDGPWIDNPFWRSKFLIEQAADVSRARSAGFEFCWIDVSLGRD 63 LK I+++ L +GMY G WI +PF R F +E D+ R + I+ + GRD Sbjct: 2 LKSIALDQLRLGMYIHSIPGGWISHPFRRKSFKVETLEDLQTLRDCPIDAITINTAKGRD 61 Query: 64 VIPGSAIDAMDVAAEPRVELVPLTPEDEYRN---------ELSEALRLYESAKVSTQQMF 114 V + ++ + + RN E A R+ S+K + MF Sbjct: 62 VTR----EELEEDDADQDAPAAPPADAANRNPASRTDTAIERERAQRIISSSKSTVLHMF 117 Query: 115 RQARLGKTLDVAQCQDLVEEIAASVTRNSNALLSIIRLKRADEYTYLHSIAVCTMMVALA 174 +AR+GK +DV +LV EI ASV+RN++AL+S+ RLK D+YTYLHS+AVC MM++LA Sbjct: 118 SEARMGKAIDVKDAAELVTEITASVSRNADALISLARLKTTDDYTYLHSVAVCAMMISLA 177 Query: 175 RTLGMDNTACKEAGLAGYLHDVGKAFIPLDVLNKPGKLTDQEFRIIQRHTTLGYEYLSGF 234 LGM K+AG+AG LHDVGK +PLD+LNKP KLT++EF ++ HT G+ L Sbjct: 178 NQLGMTPQQTKQAGMAGLLHDVGKMAVPLDILNKPAKLTEEEFASVRMHTVQGHGILQQI 237 Query: 235 AGVPDYVLDVCLHHHEKIDGTGYPGRQQGNQIDLYSRMAAVCDVYDAITSDRTYKSAWDP 294 G+ + LDV LHHHEKIDG+GYP + + I + ++M AVCDVYDAITS+R YK+ WDP Sbjct: 238 EGIGEVALDVSLHHHEKIDGSGYPFNLKADSISIMAKMGAVCDVYDAITSNRPYKAGWDP 297 Query: 295 AESLARMASWSGHFDRPIFLTFVKTLGIYPIGSIVKLRSGRTALVIRQNHKDLTKPVVKA 354 A S+ MA+ +GHFD FVK +GIYP GS V L+SGR +V+ Q L P VK Sbjct: 298 ARSIRHMAASAGHFDPITMQAFVKAIGIYPTGSCVMLQSGRLGVVVDQRPDQLLTPRVKV 357 Query: 355 FTTALSIESLESEIIDLS 372 F + + LESEIIDL+ Sbjct: 358 FYSTANRMPLESEIIDLA 375 >gi|300311984|ref|YP_003776076.1| HD-GYP domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 406 Score = 325 bits (833), Expect = 5e-90, Method: Compositional matrix adjust. Identities = 161/377 (42%), Positives = 231/377 (61%), Gaps = 10/377 (2%) Query: 4 LKKISVNDLAVGMYFSGFDGPWIDNPFWRSKFLIEQAADVSRARSAGFEFCWIDVSLGRD 63 LK I+ L +GMY F G W P WR FL+E+ D+ +++ ID++ G D Sbjct: 2 LKFITPKQLRIGMYIQSFSGAWRRPPAWRKPFLLEKDKDLLALQASPIREIVIDIAKGAD 61 Query: 64 VIP-----GSAIDAMDVAAEPRVELVPLTPEDEYRNELS----EALRLYESAKVSTQQMF 114 V G+ +++ + +E+ PE R ++ A R+ S++ + MF Sbjct: 62 VQTDEAEQGNGVES-SLGKNAEIEVAQALPEAGKRTDIQAERERAARIISSSEKTVLTMF 120 Query: 115 RQARLGKTLDVAQCQDLVEEIAASVTRNSNALLSIIRLKRADEYTYLHSIAVCTMMVALA 174 QAR+GKT+DV + DLV EI ASV+RN++A++S++RLK AD YTY+HS+AVC MM++LA Sbjct: 121 SQARMGKTIDVKEASDLVREINASVSRNADAMISLVRLKTADNYTYMHSVAVCAMMISLA 180 Query: 175 RTLGMDNTACKEAGLAGYLHDVGKAFIPLDVLNKPGKLTDQEFRIIQRHTTLGYEYLSGF 234 LG+ ++AG+AG LHD+GK +P +LNKP KLT++EF +Q H G+ L Sbjct: 181 DQLGLSAQERQQAGMAGLLHDIGKMTVPPSILNKPSKLTEEEFVSVQSHPLAGHRLLQEL 240 Query: 235 AGVPDYVLDVCLHHHEKIDGTGYPGRQQGNQIDLYSRMAAVCDVYDAITSDRTYKSAWDP 294 G+ D LDV LHHHEK+DGTGYP + G I + +RM A+CDVYDAITSDR YKS WDP Sbjct: 241 PGIGDAPLDVSLHHHEKMDGTGYPYKLSGRDISMTARMGAICDVYDAITSDRPYKSGWDP 300 Query: 295 AESLARMASWSGHFDRPIFLTFVKTLGIYPIGSIVKLRSGRTALVIRQNHKDLTKPVVKA 354 + S+ MA GHFD + FVK +GIYP+GS V +++G A+V+ Q L P+V+ Sbjct: 301 SRSIQHMAQSKGHFDFDVLQAFVKAVGIYPVGSCVLMQTGHLAMVLEQRPDSLLAPLVRI 360 Query: 355 FTTALSIESLESEIIDL 371 F + ++ +IDL Sbjct: 361 FYSTNKKIAVTPTLIDL 377 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,271,380 Number of extensions: 51377 Number of successful extensions: 150 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 143 Number of HSP's successfully gapped: 9 Length of query: 417 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 329 Effective length of database: 1,203,190 Effective search space: 395849510 Effective search space used: 395849510 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)