BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0161.p (106 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312647|ref|YP_003776739.1| 2-keto-4-pentenoate hydratase [... 163 3e-42 >gi|300312647|ref|YP_003776739.1| 2-keto-4-pentenoate hydratase [Herbaspirillum seropedicae SmR1] Length = 247 Score = 163 bits (413), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 80/105 (76%), Positives = 89/105 (84%) Query: 1 MADLQSHGALIIGAAQPLPATLSLRQIAASLHIDGQEITRTTGGNPADLWPMLGWLALHC 60 MADLQSHGAL+ GA Q LP LSLRQI ASL IDGQ+++ TTGGNPADLWP+LGWLALHC Sbjct: 142 MADLQSHGALVTGAPQALPDLLSLRQIEASLSIDGQQVSHTTGGNPADLWPLLGWLALHC 201 Query: 61 ARRGQPWCQGQVITTGSCTGLQLARAGRQIEGALAGIGAVSFSFA 105 ARRGQPW GQ+ITTGSCTGLQ+AR GR++E AL GIGAV FA Sbjct: 202 ARRGQPWRAGQIITTGSCTGLQMARGGRRVEAALHGIGAVWLDFA 246 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.135 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 375,012 Number of extensions: 15709 Number of successful extensions: 54 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 54 Number of HSP's successfully gapped: 1 Length of query: 106 Length of database: 1,619,870 Length adjustment: 72 Effective length of query: 34 Effective length of database: 1,278,950 Effective search space: 43484300 Effective search space used: 43484300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 123 (52.0 bits)