BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0159.p (343 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbasp... 644 0.0 gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Her... 139 3e-34 gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbasp... 125 5e-30 gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Her... 120 1e-28 gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Her... 115 4e-27 >gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 343 Score = 644 bits (1662), Expect = 0.0, Method: Compositional matrix adjust. Identities = 307/342 (89%), Positives = 327/342 (95%) Query: 1 MTEPLTSHLQPDSHVGTRRLGSNGPLVSAIGLGCMGMSDFYAQRDDAESLATIDRALELG 60 M+EPLTSHLQ S VGTRRLGSNGPLVSAIGLGCMGMSDFYAQRDDAESLATIDRALELG Sbjct: 1 MSEPLTSHLQAHSPVGTRRLGSNGPLVSAIGLGCMGMSDFYAQRDDAESLATIDRALELG 60 Query: 61 INLLDTADMYGPYTNEELIGRALQGRRDKFFIATKFGIRRDPANPSARGVDGSPAYIRQA 120 INLLDTADMYGPYTNEEL+GRA++GRRDKFFIATKFGIRRDP++P ARGVDGSPAYIRQA Sbjct: 61 INLLDTADMYGPYTNEELLGRAIKGRRDKFFIATKFGIRRDPSDPGARGVDGSPAYIRQA 120 Query: 121 VEGSLKRLGIETIDLYYQHRMDPNTPIEATMGVLADLVQAGKIRYIGLSEASSATLERAH 180 VEGSLKRLG+ETIDLYYQHR+DP TPIE TMGVLADLVQAGKIRYIGLSEAS+ATLERAH Sbjct: 121 VEGSLKRLGVETIDLYYQHRIDPATPIEVTMGVLADLVQAGKIRYIGLSEASAATLERAH 180 Query: 181 RVHPVTALQSEYSLWTRDPETEVLATCRALGIGFVAYSPLGRGFLTGALQRFEDLAEDDF 240 RVHPVTALQSEYSLWTRDPE EVLA CR LGIGFVAYSPLGRGFLTGA+QRFEDLAEDDF Sbjct: 181 RVHPVTALQSEYSLWTRDPEAEVLAACRTLGIGFVAYSPLGRGFLTGAIQRFEDLAEDDF 240 Query: 241 RRTNPRFQGENFARNLRLVEQVKEMAAQHGCTPSQLALAWVMAQDPHIVPIPGTKRRRYL 300 RR+NPRFQGENFARNL+L E+VKEMA QHGCTPSQLALAWVMAQDPHIVPIPGTKRRRYL Sbjct: 241 RRSNPRFQGENFARNLQLAEKVKEMAGQHGCTPSQLALAWVMAQDPHIVPIPGTKRRRYL 300 Query: 301 EENAGAFSVKLSAQDLMALEAVFPRGAAAGERYTEASMKMLN 342 E+NAG+ VKL+ +DL AL+A+FPRGAAAGERYT+ASMKMLN Sbjct: 301 EDNAGSVGVKLAPEDLQALDAIFPRGAAAGERYTDASMKMLN 342 >gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 335 Score = 139 bits (351), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 101/330 (30%), Positives = 160/330 (48%), Gaps = 22/330 (6%) Query: 18 RRLGSNGPLVSAIGLGCMGMSDFYAQRDDAESLATIDRALELGINLLDTADMYGPYTNEE 77 RRLG + VSA+ LG M D Q D ES + A E G+N +DTAD+Y +EE Sbjct: 4 RRLGKSNLKVSALCLGSMMFGD---QTDLQESGRIVASAREHGVNFIDTADVYTKGKSEE 60 Query: 78 LIGRALQGRRDKFFIATKFGIRRDPANPSARGVDGSPAYIRQAVEGSLKRLGIETIDLYY 137 ++G+ L G+R ++ +A+K G + + + S ++I + E L RL + +D+ Y Sbjct: 61 IVGKLLAGQRHEWVLASKLG---NAMSGKPNESNYSRSWIVRETENILTRLDTDYLDILY 117 Query: 138 QHRMDPNTPIEATMGVLADLVQAGKIRYIGLSE------ASSATLERAHRVHPVTALQSE 191 HR + +E + L DL++ GKIR G+S A L + V Q Sbjct: 118 MHRDYQDENLEEAVQALGDLIRVGKIRGFGVSNFRGWRIAEIVRLCEKNNVPLPLVCQPY 177 Query: 192 YSLWTRDPETEVLATCRALGIGFVAYSPLGRGFLTGALQRFEDLAED------DFRRTNP 245 Y+L R PE E+L C G+G V YSP+ RG LTG Q + A+D D R Sbjct: 178 YNLLNRQPEVEILPACAHYGLGVVPYSPIARGVLTGKYQPGQKPAQDTRAGRGDKRMMET 237 Query: 246 RFQGENFARNLRLVEQVKEMAAQHGCTPSQLALAWVMAQDPHIVPIPGTKRRRYLEENAG 305 F+ E +L + +++++ G Q A AWV+A I G + +E+ Sbjct: 238 EFREE----SLLIAQRLQQHCEARGVKLGQFATAWVLANSAISSVIAGPRTLAQMEDYYD 293 Query: 306 AFSVKLSAQDLMALEAVFPRGAAAGERYTE 335 A +K+SA++ ++++ G A+ Y + Sbjct: 294 AAELKISAEEEALVDSLVTPGHASTHGYND 323 >gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 349 Score = 125 bits (314), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 117/349 (33%), Positives = 167/349 (47%), Gaps = 50/349 (14%) Query: 18 RRLGSNGPLVSAIGLGCMGMSDFYAQRDDAESLATIDRALELGINLLDTADMYGPY---- 73 R LG VS I LG M + Q +A++ A +D A+E GINL+D A+MY P Sbjct: 4 RPLGRTDLKVSLITLGTMTWGE---QNSEADAHAQLDYAVERGINLIDVAEMY-PVPPKA 59 Query: 74 ----TNEELIGRALQ--GRRDKFFIATKF-GIRRDPANPSARGVDGSPAYI-----RQAV 121 E +G L+ G+RD+ IATK G R P NP R V G + +A+ Sbjct: 60 ETQGLTERYLGTWLKKSGKRDQLLIATKCTGPARKPHNP--RHVRGGINNLDRKGLTEAL 117 Query: 122 EGSLKRLGIETIDLY----------------YQHRMDPNT-PIEATMGVLADLVQAGKIR 164 GSL+RL ++ +DLY YQH D +T PIE T+ VL++ V+AGKIR Sbjct: 118 HGSLERLQLDHVDLYQLHWPDRSVNSFGQLGYQHVEDESTVPIEETLTVLSEFVKAGKIR 177 Query: 165 YIGLSEASS---ATLERA---HRVHPVTALQSEYSLWTRDPETEVLATCRALGIGFVAYS 218 IGLS ++ A RA H + + +Q+ Y+L R E + +G +AYS Sbjct: 178 TIGLSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFEINLAEFAHREQVGLLAYS 237 Query: 219 PLGRGFLTGALQRFEDLAEDDFRRTN--PRFQGENFARNLRLVEQVKEMAAQHGCTPSQL 276 PL G L+G ++ D A R RF A+ R + +A QHG P+Q+ Sbjct: 238 PLAFGMLSG---KYLDGARPAGGRLTLFERFSRYTNAQAERATAEYVALARQHGLDPAQM 294 Query: 277 ALAWVMAQDPHIVPIPGTKRRRYLEENAGAFSVKLSAQDLMALEAVFPR 325 ALA+V ++ I G L N + ++L L A+EA+ R Sbjct: 295 ALAYVNSRPFLTSNIIGATTLAQLRSNIDSAELQLGEDVLAAIEAIHRR 343 >gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 344 Score = 120 bits (302), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 93/291 (31%), Positives = 146/291 (50%), Gaps = 34/291 (11%) Query: 12 DSHVGTRRLGSNGPLVSAIGLGCM----GMSDFYAQRDDAESLATIDRALELGINLLDTA 67 D + RR G +G L+ AI LG G+ +F R A + RA + G+ D A Sbjct: 11 DGRMPYRRTGRSGLLLPAISLGLWQNFGGVDNFETGR------AVLRRAFDRGVTHFDLA 64 Query: 68 DMYGP--YTNEELIGRALQG----RRDKFFIATKFGIRRDPANPSARGVDGSPAYIRQAV 121 + YGP + EE GR + RD+ I++K G + P P G G+ ++ + Sbjct: 65 NNYGPPAGSAEENFGRWMAKDFLPYRDELVISSKAGWQMWPG-PYG-GPSGARKHLIASC 122 Query: 122 EGSLKRLGIETIDLYYQHRMDPNTPIEATMGVLADLVQAGKIRYIGLSEASSATLERAHR 181 E SLKR+G++ +D++Y HR+D +TP+E TMG LA L + GK Y+G+S S +A Sbjct: 123 EQSLKRMGLDYVDIFYSHRVDADTPLEETMGALAHLHRQGKALYVGISSYSPELTRKAAA 182 Query: 182 VH-----PVTALQSEYSLWTRDPETEVLATCRALGIGFVAYSPLGRGFLTGALQRFEDLA 236 + P+ Q YS+ R E +L LG+G + +SPL +G LT ++ Sbjct: 183 ILKSEGVPLLIHQPNYSMLNRAIEGGLLPALEELGVGCIGFSPLAQGLLT---SKYLGGV 239 Query: 237 EDDFRRTNP-----RFQGENFARNLRLVEQVKEMAAQHGCTPSQLALAWVM 282 + R T R E+ NL + + E+A++ G T +Q+A+AWV+ Sbjct: 240 PEGARVTRAPSLPQRMLSED---NLARIRALNEIASKRGQTLAQMAIAWVL 287 >gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 329 Score = 115 bits (289), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 92/321 (28%), Positives = 153/321 (47%), Gaps = 20/321 (6%) Query: 17 TRRLGSNGPLVSAIGLGCMGMSDFYAQRDDAESLATIDRALELGINLLDTADMYGPYTNE 76 +RR G G VS IG G + + ++ A + AL+ G++ +DTAD+YG +E Sbjct: 3 SRRFGRTGAQVSEIGFGAWAIGGSWGDVSAQDAKAALHAALDAGVSFIDTADVYGDGRSE 62 Query: 77 ELIGRALQGR-RDKFFIATKFGIRRDPANPSARGVDGSPAYIRQAVEGSLKRLGIETIDL 135 +LI + L+ R ++ F+ATK G R P S A + ++ SLK LG++ +DL Sbjct: 63 QLIAQVLKERGGERPFVATKAGRRLSPHVASGY---NDRAQLSAFIDRSLKNLGVDCLDL 119 Query: 136 YYQHRMDPNTPI---EATMGVLADLVQAGKIRYIGLSEASSATLERAHRVHPVTALQSEY 192 H P T + + G L LV AGKIR+ G+S +A V ++Q + Sbjct: 120 VQLH--CPPTEVYYQQEVFGHLDALVAAGKIRHYGVSVEKVEEALKAIEYPNVKSVQIIF 177 Query: 193 SLWTRDPETEVLATCRALGIGFVAYSPLGRGFLTGALQRFEDLAEDDFRRTN-------- 244 +++ + P + L + I +A PL G LTG + A DD R+ N Sbjct: 178 NIFRQRPISLFLQEAQRRDIAVIARVPLASGLLTGKMSAQTTFAADDHRQFNRHGEAFDV 237 Query: 245 -PRFQGENFARNLRLVEQVKEMAAQHGCTPSQLALAWVMAQDPHIVPIPGTKRRRYLEEN 303 F G + L VE+++ + Q G +Q AL W++ + IPG + + N Sbjct: 238 GETFSGVPYEVALAAVEKIRALVPQ-GVPMAQFALRWILMESGISTVIPGARNVAQAQSN 296 Query: 304 AGAFSV-KLSAQDLMALEAVF 323 + A ++ ++A+ + AL A++ Sbjct: 297 SAASALPPITAEVMQALRAIY 317 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,092,358 Number of extensions: 46188 Number of successful extensions: 156 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 141 Number of HSP's successfully gapped: 6 Length of query: 343 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 257 Effective length of database: 1,212,660 Effective search space: 311653620 Effective search space used: 311653620 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)