BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0151.p (463 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312656|ref|YP_003776748.1| alpha-ketoglutarate permease of... 868 0.0 gi|300312423|ref|YP_003776515.1| proline/betaine transporter pro... 211 8e-56 gi|300310790|ref|YP_003774882.1| major facilitator superfamily p... 200 2e-52 gi|300311827|ref|YP_003775919.1| sugar permease of the major fac... 198 6e-52 gi|300309575|ref|YP_003773667.1| proline/betaine transporter pro... 196 3e-51 >gi|300312656|ref|YP_003776748.1| alpha-ketoglutarate permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 457 Score = 868 bits (2243), Expect = 0.0, Method: Compositional matrix adjust. Identities = 441/456 (96%), Positives = 448/456 (98%) Query: 8 SAPSTTSTPLSAAEYRKRIFAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDATSQL 67 S + STPLSAAEYRKRIFAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGD TSQL Sbjct: 2 SDTTQASTPLSAAEYRKRIFAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDPTSQL 61 Query: 68 LNTAGVFAAGFLMRPIGGWLFGRIADKHGRKTSMLISVLMMCGGSLAVAVLPTYATIGAW 127 LNTAGVFAAGFLMRPIGGWLFGRIADKHGRKTSMLISVLMMCGGSLAVAV+PTYATIGAW Sbjct: 62 LNTAGVFAAGFLMRPIGGWLFGRIADKHGRKTSMLISVLMMCGGSLAVAVMPTYATIGAW 121 Query: 128 APALLLLARLFQGLSVGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLFA 187 APALLLLARLFQGLSVGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLF Sbjct: 122 APALLLLARLFQGLSVGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLFG 181 Query: 188 MQQWLTKAELMAWGWRIPFVLGAVGALVAMYLRSSLAETSSAGARKKKDAGTLKGLLQHK 247 MQQWLTKAELMAWGWR+PFVLGAVGALVAMYLRSSLAETSSAGARKKKDAGTLKGLLQHK Sbjct: 182 MQQWLTKAELMAWGWRVPFVLGAVGALVAMYLRSSLAETSSAGARKKKDAGTLKGLLQHK 241 Query: 248 RAFLNVVGFTAGGSLMFYTFTTYMQKYLVNTAGMDPKVANGVMTGALFVYMILQPIFGAI 307 RAFLNVVGFTAGGSLMFYTFTTYMQKYLVNTAGMDPKVANGVMTGALFVYMILQPIFGAI Sbjct: 242 RAFLNVVGFTAGGSLMFYTFTTYMQKYLVNTAGMDPKVANGVMTGALFVYMILQPIFGAI 301 Query: 308 SDKIGRRNSMLCFAFFGMVGTFPILHFLKDVSSPGVAMALAILALTIVSFYTSISGLIKA 367 SDKIGRRNSMLCFAFFGMVGTFPILHFLKDVSSPGVAMALAILALTIVSFYTSISGLIKA Sbjct: 302 SDKIGRRNSMLCFAFFGMVGTFPILHFLKDVSSPGVAMALAILALTIVSFYTSISGLIKA 361 Query: 368 EMFPPEVRALGVGLSYAVGNAIFGGSAEFVALSLKSAGVESAFYWYVSALCLVALIISLR 427 EMFPPEVRALGVGLSYAVGNAIFGGSAEFVALSLKSAG+ESAFYWYVSALCLVALIISLR Sbjct: 362 EMFPPEVRALGVGLSYAVGNAIFGGSAEFVALSLKSAGIESAFYWYVSALCLVALIISLR 421 Query: 428 MPDPQRDGHLKGDVAMNPDDEHGDADAASGKLHKPA 463 MPDPQRDGHLKGDVAMNPD+ G+ADAA+GKLHKPA Sbjct: 422 MPDPQRDGHLKGDVAMNPDEHQGEADAAAGKLHKPA 457 >gi|300312423|ref|YP_003776515.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 427 Score = 211 bits (538), Expect = 8e-56, Method: Compositional matrix adjust. Identities = 130/394 (32%), Positives = 212/394 (53%), Gaps = 15/394 (3%) Query: 11 STTSTPLSAAEYRKRIFAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDATSQLLNT 70 S T+ P + + + AI+ +S GN +EWFD +Y A+ A FFP GD + LL T Sbjct: 3 SNTAAPGADITHPQARRAIISSSIGNALEWFDILIYGAFAVVIAKQFFPTGDDSVSLLLT 62 Query: 71 AGVFAAGFLMRPIGGWLFGRIADKHGRKTSMLISVLMMCGGSLAVAVLPTYATIGAWAPA 130 F F MRP+G + G +D+ GRK ++++S+++M G+ +A +P+YA+IG APA Sbjct: 63 FATFGVSFFMRPLGAVVLGAYSDRAGRKAALMLSIMLMTVGTAMIAFMPSYASIGLLAPA 122 Query: 131 LLLLARLFQGLSVGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLFAMQQ 190 + L ++ QG S GGE+G+S ++ E AP+ RRGFF+S+Q + LLA L + Sbjct: 123 GIALGKMIQGFSAGGEFGSSTAFLVEHAPH-RRGFFSSWQVASQGISLLLAALFGAVLNN 181 Query: 191 WLTKAELMAWGWRIPFVLGAVGALVAMYLRSSLAETSSAGARKKKDAGTLKGLLQHKRAF 250 LT +L +WGWR+PF+ G + A +Y+R L E +K L+ H++ Sbjct: 182 MLTPEQLASWGWRVPFIFGLLIAPAGIYIRRHLDEAPEFKESSEKTNAPLRDTFAHQKMR 241 Query: 251 LNVVGFTAGGSLMFYTFTTYMQKYL----VNTAGMDPKVANGVMTGALFVYMILQPIFGA 306 L + GS++ T + Y+ Y+ V G+ + M+ A + I P+ GA Sbjct: 242 LLI----GAGSVIMATVSVYLSLYIPTYAVKQLGLPAWSSFAAMSVAGLIMFIGSPLVGA 297 Query: 307 ISDKIGRRNSMLCFAFFGMVGTFPILHFLKDVSSPGVAMAL---AILALTIVSFYTSISG 363 +SDKIGR M+ + +V T+P+ FL + SPG L ++ + + ++ ++ Sbjct: 298 LSDKIGRTPFMITSSALYIVLTYPMFVFLTN--SPGFLQLLLLQTVIGVLMTMYFAAMPA 355 Query: 364 LIKAEMFPPEVRALGVGLSYAVGNAIFGGSAEFV 397 L+ A++FP R G+ L+Y + IFGG A + Sbjct: 356 LL-ADIFPVATRGTGMSLAYNIAVTIFGGFAGLI 388 >gi|300310790|ref|YP_003774882.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 432 Score = 200 bits (508), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 134/427 (31%), Positives = 229/427 (53%), Gaps = 21/427 (4%) Query: 14 STPLSAAEYRKRIFAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDATSQLLNTAGV 73 +T + A++ K + +L +SGN +E FDF++Y F A Y + FFP + L+ T G Sbjct: 2 TTTTTNAKHSK-LSTVLRVTSGNFLEMFDFFLYGFYASYISKTFFPSDNEFVSLILTFGT 60 Query: 74 FAAGFLMRPIGGWLFGRIADKHGRKTSMLISVLMMCGGSLAVAVLPTYATIGAWAPALLL 133 F AGFLMRP+G + G D+ GR+ +++++ +M G+L +A +P YATIG AP L+L Sbjct: 61 FGAGFLMRPLGAIILGSYIDRVGRRQGLIVTLAIMAIGTLLIAFVPGYATIGVAAPILVL 120 Query: 134 LARLFQGLSVGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLFAMQQWLT 193 RL QG S G E G + Y++E+A G +GF+ S+Q + + + L+ + + Q L+ Sbjct: 121 TGRLLQGFSAGVELGGVSVYLAEMATPGNKGFYVSWQSASQQAAIMASALIGYLVSQSLS 180 Query: 194 KAELMAWGWRIPFVLGAVGALVAMYLRSSLAETSSAGARKKKDA--GTLKGLLQHKRAFL 251 ++ WGWRIPF +G + +R SL ET ARK+ + + + Q+ + + Sbjct: 181 PVQISDWGWRIPFFIGCMIVPAIFIIRRSLQETDEFLARKRHPSFGEIMSSIAQNWKIVV 240 Query: 252 NVVGFTAGGSLMFYTFTTYMQ---KYLVNTAGMDPKVANGVMTGALFVYMILQPIFGAIS 308 + ++ FY T Y K ++ + D + + + F ++ PI GA+S Sbjct: 241 AGMMMVVMTTVSFYLITVYTPTFGKNVLKLSASDSLIVTFCVGLSNFCWL---PIMGALS 297 Query: 309 DKIGRRNSMLCFAFFGMVGTFPILHFL-KDVSSPGVAMALAILALTIVSFYTSISG---L 364 D++GRR ++ F ++ +P++ +L D+S L I L + Y S +G + Sbjct: 298 DRVGRRPVLVAFTLLTLLTAYPVMSWLVSDISFKN----LLITELWLSFLYASYNGAAVV 353 Query: 365 IKAEMFPPEVRALGVGLSYAVGNAIFGGSAEFVALSL-KSAGVESA-FYWY-VSALC-LV 420 E+ P VR +G L+Y++ AIFGG V+ L +++G ++A YW +A+C L+ Sbjct: 354 ALTEVMPAHVRTVGFSLAYSLATAIFGGFTPLVSTWLIETSGDKAAPGYWMSFAAVCGLL 413 Query: 421 ALIISLR 427 A ++ R Sbjct: 414 ATLVLYR 420 >gi|300311827|ref|YP_003775919.1| sugar permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 198 bits (504), Expect = 6e-52, Method: Compositional matrix adjust. Identities = 125/414 (30%), Positives = 219/414 (52%), Gaps = 12/414 (2%) Query: 23 RKRIFAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDATSQLLNTAGVFAAGFLMRP 82 + + +L +SGN +E FDF+++ F A + + AFFP + + L+ T F AGFLMRP Sbjct: 8 QSKFSTVLRVTSGNFMEMFDFFLFGFYATHISKAFFPSDNEFASLMLTFMTFGAGFLMRP 67 Query: 83 IGGWLFGRIADKHGRKTSMLISVLMMCGGSLAVAVLPTYATIGAWAPALLLLARLFQGLS 142 +G + G D+ GR+ +++++ +M G++ +A +P+YATIGA AP L+L+ RL QG S Sbjct: 68 LGAIILGAYVDRVGRRQGLIVTLALMALGTMLIAFVPSYATIGALAPLLVLIGRLLQGFS 127 Query: 143 VGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLFAMQQWLTKAELMAWGW 202 G E G + Y++E+A G +GF+ S+Q + ++A + + + LT AE+ WGW Sbjct: 128 AGVELGGVSVYLAEMATPGNKGFYVSWQSASQQVAIIVAAAIGYLLSTSLTPAEVSGWGW 187 Query: 203 RIPFVLGAVGALVAMYLRSSLAETSSAGARKKKDA--GTLKGLLQHKRAFLNVVGFTAGG 260 RIPF +G + V +R SL ET RK + + + ++++ + + + + Sbjct: 188 RIPFFIGCLIVPVLFVIRRSLQETEEFMRRKHRPSTGQIFRSMIENWKLVIAGMMLVSMT 247 Query: 261 SLMFYTFTTYMQKYLVNTAGMDPKVANGVMTGALFVYMILQPIFGAISDKIGRRNSMLCF 320 ++ FY T Y + N + + + V I PI GA+SD++GRR ++ F Sbjct: 248 TVSFYLITVYTPTFGKNVLKLSTEASLIVTLCVGLSNFIWLPIMGALSDRVGRRPLLVTF 307 Query: 321 AFFGMVGTFPILHFLKDVSSPGVAMALAILALTIVSFYTSISGLIK---AEMFPPEVRAL 377 ++ +P +++L +S + I+ L + Y S +G + E+ P +VR Sbjct: 308 TVLTILTAYPAVNWLVHDASFA---KMLIVELWLSFLYASYNGAMVVALTEVMPADVRTA 364 Query: 378 GVGLSYAVGNAIFGGSAEFVALSLKSAGVESA---FYWYVSALC-LVALIISLR 427 G L+Y++ A+FGG +A L + A + +A+C L+A +I R Sbjct: 365 GFSLAYSLATAVFGGFTPAIATGLIEYTHDKAAPGIWMSTAAVCGLIATLILYR 418 >gi|300309575|ref|YP_003773667.1| proline/betaine transporter protein [Herbaspirillum seropedicae SmR1] Length = 433 Score = 196 bits (499), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 132/420 (31%), Positives = 222/420 (52%), Gaps = 16/420 (3%) Query: 21 EYRKRIFAILGASSGNLVEWFDFYVYSFCAIYFAPAFFPKGDATSQLLNTAGVFAAGFLM 80 + R+R AI+ GN +EWFDF VYSF A+ A FFP G+ + LL F GF M Sbjct: 17 QQRRR--AIVATVIGNGLEWFDFTVYSFFAVIIAKLFFPTGNDLTSLLLAVATFGVGFFM 74 Query: 81 RPIGGWLFGRIADKHGRKTSMLISVLMMCGGSLAVAVLPTYATIGAWAPALLLLARLFQG 140 RP+GG L G AD+ GRK ++ +++L+M G+ + + PTY +IG +AP ++++ARL QG Sbjct: 75 RPVGGILLGIYADRVGRKAALSVTILLMAVGTTMIGLAPTYESIGLFAPLIIVVARLLQG 134 Query: 141 LSVGGEYGTSATYMSEVAPNGRRGFFASFQYVTLIGGQLLAVLVLFAMQQWLTKAELMAW 200 S GGE G++ +++E AP R +++S+ ++ LL V + L A L +W Sbjct: 135 FSAGGEMGSATAFLTEYAPERERAYYSSWIQASIGVAVLLGAAVGTFVTASLDAAALASW 194 Query: 201 GWRIPFVLGAVGALVAMYLRSSLAETSSAGARKKKDAGTLKGLLQH-----KRAFLNVVG 255 GWR+PF+LG + V Y+R L ET + K K LK ++++ +F V+ Sbjct: 195 GWRLPFLLGIIIGPVGYYIRHHLEETPTFLEEKDKTDSPLKEVIRNFPRETSASFSMVIL 254 Query: 256 FTAGGSLMFYTFTTYMQKYLVNTAGMDPKVANGV--MTGALFVYMILQPIFGAISDKIGR 313 +T ++ + TY K L P+ + M G L + M+L PI G ++D+IGR Sbjct: 255 WTVCTYVLLFYMPTYSVKVL-----KLPQTTGFIAGMVGGLCI-MVLAPIVGRLADRIGR 308 Query: 314 RNSMLCFAFFGMVGTFPILHFLKDVSSPGVAMALAILALTIVSFYTSISGLIKAEMFPPE 373 R + A ++ +P+ ++ S + ++ T+++ YT +E+FP + Sbjct: 309 RPLLSGSAALILMLAYPMFAYINSAPSLSTLLIFQLVFGTLIAAYTGPILAAFSELFPAK 368 Query: 374 VRALGVGLSYAVGNAIFGGSAE-FVALSLKSAGVESAFYWYVSALCLVALIISLRMPDPQ 432 V + G+ ++Y + IFGG A F+ + + G A +YV ++ + + + +PQ Sbjct: 369 VLSTGLSVAYNLAVTIFGGFASFFITWLIAATGSTMAPAFYVMIAAAISFVGTRFVREPQ 428 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.138 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,372,787 Number of extensions: 56981 Number of successful extensions: 339 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 302 Number of HSP's successfully gapped: 16 Length of query: 463 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 374 Effective length of database: 1,198,455 Effective search space: 448222170 Effective search space used: 448222170 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 132 (55.5 bits)