BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0144.p (177 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312665|ref|YP_003776757.1| membrane protein [Herbaspirillu... 286 7e-79 >gi|300312665|ref|YP_003776757.1| membrane protein [Herbaspirillum seropedicae SmR1] Length = 177 Score = 286 bits (732), Expect = 7e-79, Method: Compositional matrix adjust. Identities = 146/177 (82%), Positives = 157/177 (88%) Query: 1 MANNNASPSLFARLLRHLRMTRAAARRIFPSSTLKAIQASIGEGERQHRAQVRVIVEAAL 60 M +NN SPSL ARLL HLRMTRAAARR FPS+TLKAIQASIG GER+HRAQVRVIVEAAL Sbjct: 1 MPHNNTSPSLPARLLHHLRMTRAAARRAFPSTTLKAIQASIGAGEREHRAQVRVIVEAAL 60 Query: 61 TLGAVRRGESARQRAHELFSLYRIWDTEENCGVLVYVNLADRKVEIVADRGINACVTREQ 120 +LGAVRRG+SARQRAHELFS YRIWDTEENCGVLVY+NLADRKVEIVADRG+NA VTREQ Sbjct: 61 SLGAVRRGDSARQRAHELFSHYRIWDTEENCGVLVYINLADRKVEIVADRGVNARVTREQ 120 Query: 121 WQQVCRQMTAGYARGDYEASTLQALAALHALLESVLPRRNDGNAKATNELSDKPLLL 177 WQQVCR MTAGYA+G+YEASTLQALA+LH +L VLPR D NELSDKPLLL Sbjct: 121 WQQVCRAMTAGYAQGEYEASTLQALASLHEILRKVLPREPDAAGDMHNELSDKPLLL 177 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.131 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 465,987 Number of extensions: 15400 Number of successful extensions: 44 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 44 Number of HSP's successfully gapped: 1 Length of query: 177 Length of database: 1,619,870 Length adjustment: 79 Effective length of query: 98 Effective length of database: 1,245,805 Effective search space: 122088890 Effective search space used: 122088890 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)