BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0142.p (393 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312570|ref|YP_003776662.1| hypothetical protein Hsero_3273... 57 4e-09 >gi|300312570|ref|YP_003776662.1| hypothetical protein Hsero_3273 [Herbaspirillum seropedicae SmR1] Length = 386 Score = 56.6 bits (135), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 75/296 (25%), Positives = 128/296 (43%), Gaps = 45/296 (15%) Query: 18 GMSRQTFSADYQEKQPLLIRNAVHHIDFSWTDACEILERCHVDDPSFKLSYHGSILPKKD 77 G++ F DY K+PLLIRNA+ E+ + DD +L H Sbjct: 22 GITPAQFLRDYWHKKPLLIRNAIPGFSAPLPRQ-ELFDLSRRDDVESRLITH-------- 72 Query: 78 YVERYLDVGTPSYRLIKPVVYDYMRKGATLIANRIK-HEPKVSAFARAVGEFTGRQV--V 134 E D+ + + P+ +K TL+ + H P A R ++ + Sbjct: 73 -REGGWDMQHGPFNKLPPL----KQKAWTLLVQGVNLHHPAADALLRQFRFVPDARLDDL 127 Query: 135 SSAYAAFGHHSSFRSHWDTRDVFAVQLIGRKRWLIYAPTFEFPLSHQQSRDYELTFPHSS 194 +YA+ G H+D+ DVF +Q G++RW I A + L+ ++ ++ + Sbjct: 128 MISYASDG--GGVGPHFDSYDVFLLQAHGQRRWRISA---QDDLTLEEGVPLKILSHFQA 182 Query: 195 EPYLDLVLEPGDVLYVPRGWWHDPLPLGEGCFHLALGTFPPYVADYLRWATHQ-IQENVL 253 E + VLEPGD+LY+P + HD + +GE C ++G R T+Q + E+ L Sbjct: 183 EE--EFVLEPGDMLYLPPHYAHDGVAVGE-CMTYSIG---------FRAPTYQELGESFL 230 Query: 254 ARRSLDTWEFEQELLEKAAQEMAHFIRSSENYAQFMDEYCAHVRDESALDLGRFAD 309 EF + ++ + +++S A+ + A +RDE L+ RF D Sbjct: 231 --------EFMMDTIDLPGRYADPDLKTSARPAELQADMIARIRDE--LNKIRFTD 276 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.139 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,268,795 Number of extensions: 55616 Number of successful extensions: 152 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 151 Number of HSP's successfully gapped: 1 Length of query: 393 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 306 Effective length of database: 1,207,925 Effective search space: 369625050 Effective search space used: 369625050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)