BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0140.p (421 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312188|ref|YP_003776280.1| hemolysin secretion-related tra... 157 2e-39 gi|300312349|ref|YP_003776441.1| hemolysin-type secretion transm... 122 5e-29 gi|300311130|ref|YP_003775222.1| Type I antifreeze protein:HlyD ... 103 4e-23 >gi|300312188|ref|YP_003776280.1| hemolysin secretion-related transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 447 Score = 157 bits (397), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 129/438 (29%), Positives = 211/438 (48%), Gaps = 76/438 (17%) Query: 1 LLVVLSIWAYLARIDVAASAAGKLVPEGKTQLLHATEMSLVRAIHAREGQRVKAGDLIVE 60 +++ +W+ L RID+ SA GK++P +T+ + + ++S VRAI EGQ VKAG++++E Sbjct: 69 MVIFALLWSLLGRIDIVVSANGKVIPSARTKTIASVDVSAVRAILVSEGQLVKAGEVLIE 128 Query: 61 LDDTINRAEYK----AVTQKLIQLRLERERLQAELDEREP----VFDVADALPESIAAQR 112 LD + + AE AV Q +Q + ++A + R P V VA A E++ +Q Sbjct: 129 LDSSSSDAERDKAADAVAQARLQAARAQAMMEAIQNLRTPELAQVPGVAQAQWEAVRSQ- 187 Query: 113 ALFTAQRHAHQSRVAEAERNRQHRLMQQAAATALIAKLQANLQSARERVERVRPY-AGES 171 AQ ++R+ + I++ A L+ A +R + A + Sbjct: 188 --LVAQYQDFRARLGRLDDE--------------ISRYGAALKLATQRASDYQTLVADHT 231 Query: 172 IAHFEYLRLQDNANSLAGELASQRASAQAARQEHLAAEQRLQFLEAEHRSSLLAQTQEKA 231 ++ +L + L G+L R R++L+AQT++ A Sbjct: 232 VSRHAWLEKEQARVDLQGQLDDAR----------------------NQRATLVAQTRKDA 269 Query: 232 --------ALMVAAQSDQARIEQLLRQKRLRSPVDGQVQNLSVATLGGAVTPGQVVASIV 283 + A+Q DQ R ++ + +L +PV G VQ L+V T+GG V Q + IV Sbjct: 270 HDSRIEADKTIEASQQDQRRADEHSKLLKLVAPVTGTVQQLTVHTIGGVVPAAQPLMQIV 329 Query: 284 PEGMPLLIEAALSNEDAGFVRVGQRVDIKVDAFPYQQYGTLPGVVTWISPDAEPRGAGGN 343 P+ + +EA L N+D GFV +GQ ++K+DAF Y +YGTLP V IS DA Sbjct: 330 PQDSQIEVEAMLENKDVGFVEIGQAAEVKIDAFDYTKYGTLPARVVHISRDA-------- 381 Query: 344 DAAAVATGMEPSSPPARGGLMYRMHIKPESTSLMRNGARHLVGIGMSVQADIITDRRRVI 403 + GL+Y I + +SL+ +G + + GM+ +I T RRVI Sbjct: 382 ------------IQDEKKGLLYSARIVLDQSSLIVDGRKVQLSAGMAANVEIKTGNRRVI 429 Query: 404 EFLLAPIIKNLDEGLKAR 421 E+ L+P+I++ E L R Sbjct: 430 EYALSPLIRHQKEALHER 447 >gi|300312349|ref|YP_003776441.1| hemolysin-type secretion transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 386 Score = 122 bits (307), Expect = 5e-29, Method: Compositional matrix adjust. Identities = 121/427 (28%), Positives = 194/427 (45%), Gaps = 71/427 (16%) Query: 2 LVVLSIWAYLARIDVAASAAGKLVPEGKTQLLHATEMSLVRAIHAREGQRVKAGDLIVEL 61 ++VL +WA +++ID A G+++ +TQ + A + V+ I +EG+ VK G L++ L Sbjct: 24 VLVLLLWAAVSQIDQVTRAQGQVIAVSRTQSVQAPDGGPVKQILVKEGEAVKQGQLLMVL 83 Query: 62 DDTINRAEYKAVTQKLIQLRLERERLQAELDEREPVF--DVADALPESIAAQRALFTAQR 119 + + K+ LR+ RL+AE+ F D+ D E IA Q+ L+ Sbjct: 84 EQDRVQTSLNDSRAKVAALRISLARLRAEVYGHPLNFEPDLLD-YKEYIANQKDLYV--- 139 Query: 120 HAHQSRVAEAERNRQHRLMQQAAATALIAKLQANLQSARERVERVRPY--AGESIAHFEY 177 ++ L+ Q +A LQ L A + +E + G+ + + Sbjct: 140 -------------KRKILIDQD-----LASLQDMLGLANQELEMNKSLERTGD-VGRADV 180 Query: 178 LRLQDNANSLAGELASQRASAQAARQEHLAAEQRLQFLEAEHRSSLLAQTQEKAALMVAA 237 LRLQ N + ++ ++R QE L ++Q R L +T Sbjct: 181 LRLQRNVADIRAQINNKR---NKYFQEALVDMTKVQEDLNTQREQLEDRT---------- 227 Query: 238 QSDQARIEQLLRQKRLRSPVDGQVQNLSVATLGGAVTPGQVVASIVPEGMPLLIEAALSN 297 Q+L L++P DG V+N+ V T+GG V G VV I+P L++EA +S Sbjct: 228 --------QVLEHTELKAPADGVVKNIKVTTIGGVVRSGDVVMEILPVD-NLIVEAKVST 278 Query: 298 EDAGFVRVGQRVDIKVDAFPYQQYGTLPGVVTWISPDAEPRGAGGNDAAAVATGMEPSSP 357 D+ +V+VGQ ++K+DA+ Y YGTL G V ++SPD T ++ + Sbjct: 279 ADSAYVKVGQAANVKLDAYDYSIYGTLNGHVVYVSPD---------------TLLDET-- 321 Query: 358 PARGGLM--YRMHIKPESTSLMRNGARHL-VGIGMSVQADIITDRRRVIEFLLAPIIKNL 414 R G M YR+HI E A + V GM+ +I + R V+ FL PI K L Sbjct: 322 --RQGAMPYYRVHIALEEAVFKGRKADAIQVKPGMTATVEIKANTRTVLSFLTKPIAKTL 379 Query: 415 DEGLKAR 421 E + R Sbjct: 380 QESMGER 386 >gi|300311130|ref|YP_003775222.1| Type I antifreeze protein:HlyD family secretion protein [Herbaspirillum seropedicae SmR1] Length = 457 Score = 103 bits (256), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 120/438 (27%), Positives = 192/438 (43%), Gaps = 85/438 (19%) Query: 8 WAYLARIDVAASAAGKLVPEGKTQLLHATEMSLVRAIHAREGQRVKAGDLIVELDDTINR 67 W+ A ID+ +G++V Q++ E +V AI REG VK G ++ + D Sbjct: 58 WSVFAPIDMVVRGSGRIVASEHNQVIQHLEGGIVSAILVREGAAVKKGQTLISISDVRAN 117 Query: 68 AEYKAVTQKLIQLRLERERLQAELDEREPV---FDVADALPESIAAQRALFTAQRHAHQS 124 A+ K++ LR + RL AE + D+ P ++ +++A F A Sbjct: 118 ADLGEGRVKILGLRAKIARLSAEASGASSLQMPDDLERGDP-AVQSEQAAFAA------- 169 Query: 125 RVAEAERNRQHRLMQQAAAT-ALIAKLQANLQSARERVERVRPYAGE-SIAHFEYLRLQD 182 R +LMQ+ + A+ QA L A + R + A E +A +Y + D Sbjct: 170 --------RSSKLMQEMSVLREQSAQRQAELGEA---ISRQKSQASELELAQRQYKLIHD 218 Query: 183 NANSLAGELASQRASAQAAR-----QEHLAA-------------EQRLQFLEAEHRSSLL 224 L ASQ QAA Q LAA E + + EA R Sbjct: 219 ---MLVRNAASQLEDIQAASRVQDAQSKLAATNAMIPRLNAAIAEAQAKQGEAASRFRSD 275 Query: 225 AQTQEKAALMVAAQSDQARIEQLLRQKR-------LRSPVDGQVQNLSVATLGGAVTPGQ 277 A+T+ + AA+++ AR+++ ++ + +++P+DG V + + T+GG V PG Sbjct: 276 ARTE-----LTAAETELARLQEEIKSRNDRVSRSEVKAPMDGVVNRVMINTIGGVVRPGD 330 Query: 278 VVASIVPEGMPLLIEAALSNEDAGFVRVGQRVDIKVDAFPYQQYGTLPGVVTWISPDAEP 337 + + P L+IEA +S D + G R +KV A+ Y YG + GVVT IS D P Sbjct: 331 PIIEMTPSDDKLVIEARISPTDRAELVTGARARVKVSAYDYGVYGAMDGVVTEISADTVP 390 Query: 338 RGAGGNDAAAVATGMEPSSPPARGGLMYRMHIKPESTSLMRNGARHL-----VGIGMSVQ 392 + SP RG YR+ I+ ++ G L + GM+ Sbjct: 391 ---------------DEGSP--RGERFYRLKIE------VKRGHDELQQKLALMPGMTAS 427 Query: 393 ADIITDRRRVIEFLLAPI 410 ADII RR V ++L++P+ Sbjct: 428 ADIIVGRRTVWQYLMSPL 445 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.130 0.355 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,141,648 Number of extensions: 43852 Number of successful extensions: 247 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 241 Number of HSP's successfully gapped: 5 Length of query: 421 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 333 Effective length of database: 1,203,190 Effective search space: 400662270 Effective search space used: 400662270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)