BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0128.p (211 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312675|ref|YP_003776767.1| protein-glutamate methylesteras... 362 e-101 gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylester... 90 1e-19 gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylester... 83 1e-17 gi|300312321|ref|YP_003776413.1| protein-glutamate methylesteras... 77 1e-15 gi|300311029|ref|YP_003775121.1| protein-glutamate methylesteras... 63 1e-11 >gi|300312675|ref|YP_003776767.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 211 Score = 362 bits (930), Expect = e-101, Method: Compositional matrix adjust. Identities = 185/211 (87%), Positives = 194/211 (91%) Query: 1 MSLVPLPALGGRAIDAIVIGASAGGVEALSEILPALRRGAQVATMVVLHIPRERPSLLAT 60 MS +PLP L R I+AIVIGASAGGVEALSEILPALRRGA VATMVVLH+PRERPSLLA Sbjct: 1 MSELPLPELAARPIEAIVIGASAGGVEALSEILPALRRGAAVATMVVLHLPRERPSLLAQ 60 Query: 61 IFADRCGQPVVEAQDKEPAQPGVIYFAPPDYHLLVDRSDQGPVLAMSNDELVNYSRPAID 120 IFADRC QPVVEAQDKEPAQ GVI+FAPPDYHLLVDRS+ GP+LAMSNDELVNYSRPAID Sbjct: 61 IFADRCRQPVVEAQDKEPAQAGVIHFAPPDYHLLVDRSEDGPILAMSNDELVNYSRPAID 120 Query: 121 VLFESAADVYRERLLGIVLTGGNHDGAAGLQAIARAGGVTVVQSPQQAQVPYMPAQALAL 180 VLFESAADVY RLLGI+LTGGNHDGAAG+QAIARAGGVTVVQSPQQAQVPYMPAQALA+ Sbjct: 121 VLFESAADVYGPRLLGILLTGGNHDGAAGMQAIARAGGVTVVQSPQQAQVPYMPAQALAM 180 Query: 181 GQIDYVLTLPQIADLLRTLGVSGNDQEKERR 211 G IDYVLTL QIADLLRTLG SG QEKERR Sbjct: 181 GNIDYVLTLQQIADLLRTLGASGLAQEKERR 211 >gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 90.1 bits (222), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 60/174 (34%), Positives = 84/174 (48%), Gaps = 3/174 (1%) Query: 17 IVIGASAGGVEALSEILPALRRGAQV-ATMVVLHIPRERPSLLATIFADRCGQPVVEAQD 75 I+IGAS GG EA+ E L +R + ++ H+P A + C VVE+ Sbjct: 167 IIIGASTGGTEAIKEFL--IRMPSDCPGILITQHMPEGFTRSFAQRLNNLCKISVVESAG 224 Query: 76 KEPAQPGVIYFAPPDYHLLVDRSDQGPVLAMSNDELVNYSRPAIDVLFESAADVYRERLL 135 E PG Y AP HL + RS + + VN RP++DVLF+SAA + + Sbjct: 225 NERVLPGHAYIAPGHSHLKLVRSGANYMTQLDQGPPVNRHRPSVDVLFQSAATCAGKNAV 284 Query: 136 GIVLTGGNHDGAAGLQAIARAGGVTVVQSPQQAQVPYMPAQALALGQIDYVLTL 189 G++LTG DGA G+ + AG Q V MP +A+A+G VL L Sbjct: 285 GVILTGMGKDGAQGMLEMRNAGAYNFAQDEASCVVFGMPREAIAVGAAHEVLAL 338 >gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 381 Score = 83.2 bits (204), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 58/180 (32%), Positives = 89/180 (49%), Gaps = 1/180 (0%) Query: 17 IVIGASAGGVEALSEILPALRRGAQVATMVVLHIPRERPSLLATIFADRCGQPVVEAQDK 76 + IGAS GG +AL +L L + +VV H+P+ A G V EA+ Sbjct: 197 VAIGASTGGPQALERVLGKLSQRCP-GIVVVQHMPQRFTRSFAERLHRISGAEVKEAEHL 255 Query: 77 EPAQPGVIYFAPPDYHLLVDRSDQGPVLAMSNDELVNYSRPAIDVLFESAADVYRERLLG 136 + PG + AP HL+V R + + LV+ +P++DVLF S A +G Sbjct: 256 DRILPGRVLIAPGGRHLVVRREGLQYYVETLDGPLVSRHKPSVDVLFRSLAKAAGSNAVG 315 Query: 137 IVLTGGNHDGAAGLQAIARAGGVTVVQSPQQAQVPYMPAQALALGQIDYVLTLPQIADLL 196 I++TG DGA G++ +A GG T Q + V MP +A+ G + V+TL I+ ++ Sbjct: 316 IIMTGMGDDGARGMRELADCGGATYAQDEASSVVFGMPKEAIQQGGVGDVITLEHISAVI 375 >gi|300312321|ref|YP_003776413.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 76.6 bits (187), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 59/184 (32%), Positives = 93/184 (50%), Gaps = 5/184 (2%) Query: 17 IVIGASAGGVEALSEILPALRRGAQVATMVVLHIPRERPSLLATIFADRCGQPVVEAQDK 76 + +GASAGG AL+ +L A+ +G A ++V HI +A + V AQ+ Sbjct: 161 VAVGASAGGPAALATLLKAIPQGFAGAVVIVQHIDAAFAPGMAQWLQQQSALKVGLAQEG 220 Query: 77 EPAQPGVIYFAPPDYHLLVDRSDQ-GPVLAMSNDELVNYSRPAIDVLFESAADVYRERLL 135 + Q G + A + HL+V + G V ND RP++DV F+S + ++ Sbjct: 221 QAPQRGAVLLAASNRHLVVKEGGRLGYVEGAPND----VYRPSVDVFFQSIVRHWPGKVA 276 Query: 136 GIVLTGGNHDGAAGLQAIARAGGVTVVQSPQQAQVPYMPAQALALGQIDYVLTLPQIADL 195 G++LTG DGAAGL+A+ +G T+ Q + V MP A+AL VL + +A Sbjct: 277 GLLLTGMGRDGAAGLKALRDSGAHTMAQDQKSCAVYGMPKAAVALNAAVEVLPVTGMAAR 336 Query: 196 LRTL 199 L+ + Sbjct: 337 LQAI 340 >gi|300311029|ref|YP_003775121.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 353 Score = 63.2 bits (152), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 54/182 (29%), Positives = 77/182 (42%), Gaps = 1/182 (0%) Query: 17 IVIGASAGGVEALSEILPALRRGAQVATMVVLHIPRERPSLLATIFADRCGQPVVEAQDK 76 ++IG S GG L EILPAL +V H+P A C V+E Sbjct: 167 VLIGVSTGGPSTLEEILPALPASFPWPILVAQHMPAAFTRSFAERMDKLCALNVLEVAAP 226 Query: 77 EPAQPGVIYFAPPDYHLLVDRSDQGPVLAMSNDELVNYSRPAIDVLFESAADVYRER-LL 135 Q G +Y A +LV + + + + P++++L SA R L+ Sbjct: 227 TVVQGGHVYIARGGADMLVAKRGSHLSVLPKPESPTHLWHPSVELLARSALQSMAARQLI 286 Query: 136 GIVLTGGNHDGAAGLQAIARAGGVTVVQSPQQAQVPYMPAQALALGQIDYVLTLPQIADL 195 G++LTG +DGAA I GG T+ +S V MP + G VL +IA Sbjct: 287 GVMLTGMGNDGAAAFAEICGDGGRTIAESESSCVVFGMPKDLIDRGGASVVLPASKIATQ 346 Query: 196 LR 197 LR Sbjct: 347 LR 348 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 725,513 Number of extensions: 31390 Number of successful extensions: 131 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 127 Number of HSP's successfully gapped: 5 Length of query: 211 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 130 Effective length of database: 1,236,335 Effective search space: 160723550 Effective search space used: 160723550 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)