BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0118.p (228 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312682|ref|YP_003776774.1| iron-regulated protein [Herbasp... 441 e-125 gi|300312553|ref|YP_003776645.1| PKHD-type hydroxylase [Herbaspi... 240 7e-65 gi|300313662|ref|YP_003777754.1| PKHD-type hydroxylase [Herbaspi... 203 1e-53 >gi|300312682|ref|YP_003776774.1| iron-regulated protein [Herbaspirillum seropedicae SmR1] Length = 228 Score = 441 bits (1133), Expect = e-125, Method: Compositional matrix adjust. Identities = 211/228 (92%), Positives = 220/228 (96%) Query: 1 MLITIPQLLDPQALQTVRSLLDNAGPAWVDGRVTAGYQGAPVKYNQQIDEQSDVALQCQR 60 MLITIPQLLD QALQTVRSLL+ AG AWVDGRVTAGYQGAPVK+NQQIDE S+VALQCQR Sbjct: 1 MLITIPQLLDTQALQTVRSLLEQAGEAWVDGRVTAGYQGAPVKFNQQIDEHSEVALQCQR 60 Query: 61 IIVSALERHPRFISAALPNLIYPPMFNRYGEGMTFGAHVDGSVRIHPHSGRKLRTDVSAT 120 IIVSALERHPRFISAALPNLIYPPMFNRYGEGM FGAHVDGSVRIHPH+GRKLRTDVSAT Sbjct: 61 IIVSALERHPRFISAALPNLIYPPMFNRYGEGMHFGAHVDGSVRIHPHNGRKLRTDVSAT 120 Query: 121 LFLADPEDYDGGELQIDDSYGMHSVKLAAGDMVLYPATSLHTVTPVTRGVRVGCFFWVQS 180 LFLADP DYDGG+LQIDDSYG HSVKLAAGDMVLYPATSLHTVTPVTRGVR+ CFFWVQS Sbjct: 121 LFLADPGDYDGGDLQIDDSYGAHSVKLAAGDMVLYPATSLHTVTPVTRGVRLACFFWVQS 180 Query: 181 LVRDDGQRQMLFDMDNAIQRLNQTDADALARRTLVGCYHNLLRQWSET 228 LVRDDGQRQ+LFDMDNAIQRLNQT+ADALARRTLVGCYHNLLRQWS+T Sbjct: 181 LVRDDGQRQLLFDMDNAIQRLNQTEADALARRTLVGCYHNLLRQWSDT 228 >gi|300312553|ref|YP_003776645.1| PKHD-type hydroxylase [Herbaspirillum seropedicae SmR1] Length = 226 Score = 240 bits (612), Expect = 7e-65, Method: Compositional matrix adjust. Identities = 118/228 (51%), Positives = 155/228 (67%), Gaps = 2/228 (0%) Query: 1 MLITIPQLLDPQALQTVRSLLDNAGPAWVDGRVTAGYQGAPVKYNQQIDEQSDVALQCQR 60 M++ IP++L + ++ +R+ L A WVDGR T G QGA VK N+Q+ E S + + Sbjct: 1 MMLHIPEVLSAEQVRHIRAELARAD--WVDGRATVGDQGARVKRNRQLPEHSPLGFKLGE 58 Query: 61 IIVSALERHPRFISAALPNLIYPPMFNRYGEGMTFGAHVDGSVRIHPHSGRKLRTDVSAT 120 II+ AL ++P F +AALP PP+FN Y G +G HVDGSVR P++ +RTDVS+T Sbjct: 59 IILEALRKNPLFFAAALPKKTIPPLFNSYEGGEHYGLHVDGSVRNLPNAVGSIRTDVSST 118 Query: 121 LFLADPEDYDGGELQIDDSYGMHSVKLAAGDMVLYPATSLHTVTPVTRGVRVGCFFWVQS 180 LFL+DPE+YDGGEL + D+YG H VKL AGD+++YP+TSLH V PVTRG RV FFW QS Sbjct: 119 LFLSDPEEYDGGELVVVDTYGTHEVKLPAGDLIVYPSTSLHRVEPVTRGARVCSFFWTQS 178 Query: 181 LVRDDGQRQMLFDMDNAIQRLNQTDADALARRTLVGCYHNLLRQWSET 228 +VRDD +R MLF++D I L + D L G YHNLLRQW+E Sbjct: 179 MVRDDWKRSMLFELDRNIFGLREKIGDTEEVLGLTGHYHNLLRQWAEV 226 >gi|300313662|ref|YP_003777754.1| PKHD-type hydroxylase [Herbaspirillum seropedicae SmR1] Length = 227 Score = 203 bits (516), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 107/228 (46%), Positives = 150/228 (65%), Gaps = 5/228 (2%) Query: 1 MLITIPQLLDPQALQTVRSLLDNAGPAWVDGRVTAGYQGAPVKYNQQIDEQSDVALQCQR 60 M++ + Q+L + R+LL+ A W+DGRVT+G +K N+Q+ EQS A+ Q+ Sbjct: 1 MMLHLKQVLSTDEVTRARALLEQA--QWIDGRVTSGSLPDTLKQNRQLPEQSASAVPLQQ 58 Query: 61 IIVSALERHPRFISAALPNLIYPPMFNRYGEGMT-FGAHVDGSVRIHPHSGRKLRTDVSA 119 ++++AL R+ F SAALP I PMFNRYG G G HVD ++R P GR++RTDVS Sbjct: 59 MVLAALRRNTLFFSAALPRQIVTPMFNRYGAGGDHLGDHVDVAMRFMP-DGRRVRTDVSC 117 Query: 120 TLFLADPEDYDGGELQIDDSYGMHSVKLAAGDMVLYPATSLHTVTPVTRGVRVGCFFWVQ 179 TLFL++PE+Y+GGEL + D + +KL AGDM+LYPAT++H V PV G+R FFW + Sbjct: 118 TLFLSEPEEYEGGELVVADHWEDRHIKLPAGDMLLYPATTIHRVEPVRTGIRYASFFWTE 177 Query: 180 SLVRDDGQRQMLFDMDNAIQRL-NQTDADALARRTLVGCYHNLLRQWS 226 S+VR+ +RQ+LFDMD + L ++ AD A L YHNLLR W+ Sbjct: 178 SMVREGERRQLLFDMDCQLMSLRSRLAADDAAVVGLSATYHNLLRMWT 225 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 710,861 Number of extensions: 27289 Number of successful extensions: 75 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 73 Number of HSP's successfully gapped: 3 Length of query: 228 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 146 Effective length of database: 1,231,600 Effective search space: 179813600 Effective search space used: 179813600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)