BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0117.p (435 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312683|ref|YP_003776775.1| major facilitator superfamily p... 786 0.0 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 246 3e-66 gi|300311040|ref|YP_003775132.1| major facilitator superfamily p... 234 1e-62 gi|300313188|ref|YP_003777280.1| major facilitator superfamily p... 229 3e-61 gi|300312017|ref|YP_003776109.1| multidrug resistance B (translo... 160 2e-40 >gi|300312683|ref|YP_003776775.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 786 bits (2031), Expect = 0.0, Method: Compositional matrix adjust. Identities = 401/435 (92%), Positives = 415/435 (95%) Query: 1 MQTLDTTIVNTALPAMARSLAEPVLNMHPVVVAYTLTMASLTPASGWLADRFGTRRVYFA 60 MQTLDTTIVNTALPAMARSL EPVLNMHPVVVAYTLTMASLTPASGWLADRFGTRR+YFA Sbjct: 64 MQTLDTTIVNTALPAMARSLGEPVLNMHPVVVAYTLTMASLTPASGWLADRFGTRRMYFA 123 Query: 61 AILLFALGSVLCAMAPTLPMLLAARVIQGLGGSMLLPVGRLAVLRAVPGAQYIAALASIS 120 AILLF+LGS+LCAMA +LPMLLAARV+QGLGGSMLLPVGRLAVLRAVPGAQYIAALASIS Sbjct: 124 AILLFSLGSLLCAMASSLPMLLAARVVQGLGGSMLLPVGRLAVLRAVPGAQYIAALASIS 183 Query: 121 IAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVLYFLPDDSLTGTPRFDLFG 180 IAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVLYFLPDDSL GTPRFDL G Sbjct: 184 IAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVLYFLPDDSLAGTPRFDLLG 243 Query: 181 CALLSACMVAFSLALDVPFDTHRTGWSLGLFALAALTVLVYWRHASVHPRPLFRLGLFGE 240 C LLSACMVAFSLALDVPFD+HRTGWS+ LF +AALTVL YWRHASVHPRPLFRLGLFGE Sbjct: 244 CVLLSACMVAFSLALDVPFDSHRTGWSIALFVVAALTVLGYWRHASVHPRPLFRLGLFGE 303 Query: 241 PNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMLLLPAAIAGTIAKPWIAPL 300 PNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGM+LLPAAIAGTIAKPWIAPL Sbjct: 304 PNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMVLLPAAIAGTIAKPWIAPL 363 Query: 301 VKRFGYDRFLLINTAIVGISIVSFALITPGWPLAASIVQLAVFGASNSMQFAAMNSVTLK 360 VKRFGYDRFLL+NTAIVG +I+SFALITP WPL A+IVQLA+FGASNSMQFAAMNSVTLK Sbjct: 364 VKRFGYDRFLLVNTAIVGAAIMSFALITPDWPLTAAIVQLAIFGASNSMQFAAMNSVTLK 423 Query: 361 GLRKEDAGSGNSLFSMVQMLAIGLGVTIGGTMVNAFKSEAGIATNASFQITFVIVGLITL 420 GL K DAGSGNSLFSMVQMLAIGLGVTIGGTMVN FKSE G ATNASFQITF IVG ITL Sbjct: 424 GLDKRDAGSGNSLFSMVQMLAIGLGVTIGGTMVNLFKSEVGTATNASFQITFAIVGAITL 483 Query: 421 VSAWVFRKMDPRYFS 435 SAWVFRKMDPRYF Sbjct: 484 ASAWVFRKMDPRYFK 498 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 246 bits (628), Expect = 3e-66, Method: Compositional matrix adjust. Identities = 149/436 (34%), Positives = 237/436 (54%), Gaps = 17/436 (3%) Query: 4 LDTTIVNTALPAMARSLAEPVLNMHPVVVAYTLTMASLTPASGWLADRFGTRRVYFAAIL 63 +D+T+++T+LPA+A + +++ + +Y +++A P SGW+ADRFG+ ++ +AI Sbjct: 1 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 60 Query: 64 LFALGSVLCAMAPTLPMLLAARVIQGLGGSMLLPVGRLAVLRAVPGAQYIAALASISIAG 123 +F LGS+LC ++ TLP +AAR +QG+GG+M++PVGRL +LR+V + AL+ ++I Sbjct: 61 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPVGRLVILRSVEKVNLVKALSYLTIPA 120 Query: 124 QLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVLYFLPDDSLTGTPRFDL----- 178 GPV+GP LGG++ W WIF IN+P+ +LG+ F+PD DL Sbjct: 121 LFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQVAPLDLKGFLL 180 Query: 179 --FGCALLSACMVAFSLALDVPFDTHRTGWSLGLFALAALTVLVYWRHASVHPRPLFRLG 236 GC+LL + L V + T GL LAA Y HA PL L Sbjct: 181 SALGCSLLMMGLATAGRHL-VSGEVALTCVLAGLVFLAA-----YVWHARRASAPLIDLA 234 Query: 237 LFGEPNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMLLLPAAIAGTIAKPW 296 L P G+ G R+G A+PFLLPL+LQ+ GY+P H+GML +A K Sbjct: 235 LLKIPTLRAGVFGGSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAMFMKTI 294 Query: 297 IAPLVKRFGYDRFLLINTAIVGISIVSFALITPGWPLAASIVQLAVFGASNSMQFAAMNS 356 A ++R+G+ R LL+N A+ + + + A TP P +V L + G S+QF ++N+ Sbjct: 295 AARFLRRYGFRRVLLVNGALGVLGLWAIATFTPQTPYLLMVVILLIGGCFRSLQFTSLNA 354 Query: 357 VTLKGLRKEDAGSGNSLFSMVQMLAIGLGVTIGG---TMVNAFKSEAGIATNASFQITFV 413 ++ + K S+ S+ Q L+IG+GV + G M N + A + F F+ Sbjct: 355 ISYADVDKLRLSQATSMASVAQQLSIGMGVVLAGFALQMSNEIQGHATLVAQ-DFWPAFL 413 Query: 414 IVGLITLVSAWVFRKM 429 +VG + + + R++ Sbjct: 414 VVGAVAACALLMLRQL 429 >gi|300311040|ref|YP_003775132.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 234 bits (596), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 141/434 (32%), Positives = 242/434 (55%), Gaps = 13/434 (2%) Query: 4 LDTTIVNTALPAMARSLAEPVLNMHPVVVAYTLTMASLTPASGWLADRFGTRRVYFAAIL 63 +D T++ T+LPA+AR L + + + + AY + P SGW+AD+ G R+V+ AA++ Sbjct: 1 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGWMADKLGARKVFSAAMI 60 Query: 64 LFALGSVLCAMAPTLPMLLAARVIQGLGGSMLLPVGRLAVLRAVPGAQYIAALASISIAG 123 +F GS+LCA++ +L +AAR +QG+GG+M++PVGR+ + R+V ++ + A++ +++ Sbjct: 61 VFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTLPA 120 Query: 124 QLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVLYFLPDDSLTGTPRFDLFGCAL 183 LGPVVGP+LGG++ W W+FLIN+P LG+ F+ + D+ G L Sbjct: 121 LLGPVVGPLLGGFITTYFHWRWVFLINIPFCFLGLYLARRFIQNFKEDNPAPLDVKGFVL 180 Query: 184 LS--ACMVAFSLALDVPFDTHRTGWS--LGLFALAALTVLVYWRHASVHPRPLFRLGLFG 239 S A M+ L+L F +H + + + ++ A+ +++Y+RH++ PL L Sbjct: 181 TSVGAAMIMLGLSL---FGSHLVDGATPVAMCSIGAIALVMYYRHSTQIEFPLLDFRLLR 237 Query: 240 EPNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMLLLPAAIAGTIAKPWIAP 299 P + G + RIG AVPFLLPL LQ LG + AG + +A + Sbjct: 238 IPTLHASVVGGSLFRIGLGAVPFLLPLALQEGLGMTAFQAGSITCASAFGSLFIRLLAGR 297 Query: 300 LVKRFGYDRFLLINTAIVGISIVSFALITPGWP--LAASIVQLAVFGASNSMQFAAMNSV 357 +++R+G+ L++N GI++ ++ + + P L SIV + G ++QF +NS+ Sbjct: 298 VLRRYGFRTVLMVNAVFAGIALAAYGIFSIHTPYLLIVSIVLMG--GVFPALQFTCLNSI 355 Query: 358 TLKGLRKEDAGSGNSLFSMVQMLAIGLGVTIGGTMVNAFKSEAGIAT--NASFQITFVIV 415 + DAG SL S++Q L++GLGVT+ G ++ G AT A F F+++ Sbjct: 356 AYADIDSRDAGRVTSLASVIQQLSLGLGVTVAGIVLQLSNHLQGHATIVEADFWPAFLVI 415 Query: 416 GLITLVSAWVFRKM 429 GL ++ S + K+ Sbjct: 416 GLFSVASVPISGKL 429 >gi|300313188|ref|YP_003777280.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 229 bits (585), Expect = 3e-61, Method: Compositional matrix adjust. Identities = 146/438 (33%), Positives = 233/438 (53%), Gaps = 9/438 (2%) Query: 1 MQTLDTTIVNTALPAMARSLAEPVLNMHPVVVAYTLTMASLTPASGWLADRFGTRRVYFA 60 M+ LDTTI+ TALP MA+S + + AY LT+A P SGW+ADRFG+R V+ Sbjct: 42 MEYLDTTIIATALPQMAQSFGVGPNELSLGMTAYMLTLAVFIPISGWVADRFGSRTVFGM 101 Query: 61 AILLFALGSVLCAMAPTLPMLLAARVIQGLGGSMLLPVGRLAVLRAVPGAQYIAALASIS 120 AI +F L S+LC + + AARV+QGLGG+M++PVGR+ V+R+ + + A+A+I+ Sbjct: 102 AIAIFTLASILCGFSQNVWEFTAARVLQGLGGAMMVPVGRMIVVRSTGKDRLMKAIATIT 161 Query: 121 IAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVLYFLPDDSLTGTPRFDLFG 180 + PVVGP +GG++ SWHWIFL+N+P G+ AV+ + ++ DL G Sbjct: 162 WPAIVAPVVGPSIGGFITTYASWHWIFLMNVPFGLAAFTAVMMVVRNEHGVSKRPLDLVG 221 Query: 181 CALLSACMVAFSLALDVPFDTHRT-GWSLGLFALAALTVLVYWRHASVHPRPLFRLGLFG 239 L A + + ++ +LG AL + WRH PL Sbjct: 222 FLLSGAALTSLLYGTEIASHMESNIKVALGFVALGVVLGYGAWRHMQRIEHPLLDFSNLR 281 Query: 240 EPNFSIGLAGNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMLLLPAAIAGTIAKPWIAP 299 P +S+ + + RIG AVP+L+PL+ Q+ G + +G+LLL A+ K + Sbjct: 282 IPTYSVTVLTGSLTRIGVDAVPYLMPLMFQIGFGLTAFQSGLLLLATALGNLGMKAFTTR 341 Query: 300 LVKRFGYDRFLLINTAIVGISIVSFALITPGWPLAASIVQLAVFGASNSMQFAAMNSVTL 359 +++RFG+ + N AI G I++ L+TP PL + + +G S SMQF+ + ++ Sbjct: 342 ILQRFGFRSVAVNNAAIAGGLIIACGLLTPATPLPVILGVIFCYGLSRSMQFSTLATLAY 401 Query: 360 KGLRKEDAGSGNSLFSMVQMLAIGLGVTIGGTMVNAFKSEAGIATNAS--------FQIT 411 + S ++L+S+ Q + IG+G+ G + + G A +AS FQ Sbjct: 402 ADISDARKSSASTLWSVAQQMTIGMGIAFGALALRIAAALRGEAIDASGAHFTLGDFQWA 461 Query: 412 FVIVGLITLVSAWVFRKM 429 F + G++TL+S +R + Sbjct: 462 FAMAGVVTLISVIGYRGL 479 >gi|300312017|ref|YP_003776109.1| multidrug resistance B (translocase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 524 Score = 160 bits (405), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 135/413 (32%), Positives = 196/413 (47%), Gaps = 35/413 (8%) Query: 1 MQTLDTTIVNTALPAMARSLAEPVLNMHPVVVAYTLTMASLTPASGWLADRFGTRRVYFA 60 M LD++I N A+P ++ +L V V+ ++ A P +GWL R G R++ Sbjct: 35 MNVLDSSIANVAIPTISGNLGVSVDEGTWVITSFAAANAISIPLTGWLTQRLGAVRLFVT 94 Query: 61 AILLFALGSVLCAMAPTLPMLLAARVIQGLGGSMLLPVGRLAVLRAVPGAQYIAALASIS 120 ++LLF L S LC +APTLP+LLAARV+QGL ++P+ + +L A P ++ ALA Sbjct: 95 SVLLFVLASWLCGLAPTLPILLAARVLQGLVAGPMVPLSQSLLLGAYPKSKSSFALALWG 154 Query: 121 IAGQLGPVVGPVLGGWLVESLSWHWIFLINLPVGVLGMGAVL-YFLPDDSLTGTPRFDLF 179 + + PV GP LGGW+ +S +W WIF IN+PVG++ G L + ++ T D Sbjct: 155 MTAVVAPVAGPALGGWITDSYTWPWIFYINIPVGLIAAGVTLAIYRSRETPTHKLPIDKI 214 Query: 180 GCALLSACMVAFSLALDVPFD----THRTGWSLGLFALAALTVLVYWRHASVHPRPLFRL 235 G ALL + AF + LD D T +LG+ AL A V W + H P+ L Sbjct: 215 GLALLVTWVAAFQIMLDKGKDLDWFASPTIVTLGIIALIAFAYFVVWELNNDH--PVVDL 272 Query: 236 GLFGEPNF-------SIGLA---GNLVCRIGSSAVPFLLPLLLQVRLGYSPLHAGMLLLP 285 LFG NF SIG GNLV +LP LQ LGY + +G+ P Sbjct: 273 RLFGRRNFAGGTIAISIGYGVFFGNLV----------ILPQWLQQYLGYRSIDSGLSTAP 322 Query: 286 AAIAGTIAKPWIAPLVKRF--GYDRFLLINTAIVGISIVSFALITPGWPLAASIVQLAVF 343 I I + PL+ RF D + + +G +IV + + V L Sbjct: 323 IGIFAVI----LMPLIGRFLPLVDARKVATASFIGFAIVFWLRSRYNLQVDQMTVILPTL 378 Query: 344 --GASNSMQFAAMNSVTLKGLRKEDAGSGNSLFSMVQMLAIGLGVTIGGTMVN 394 G +M F + + L GL E + L S V++ +G +I T N Sbjct: 379 LQGIPTAMFFVPLTVLLLSGLPPERIPAAAGLSSFVRVFCGAVGTSISTTAWN 431 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.141 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,314,569 Number of extensions: 54259 Number of successful extensions: 398 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 376 Number of HSP's successfully gapped: 19 Length of query: 435 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 347 Effective length of database: 1,203,190 Effective search space: 417506930 Effective search space used: 417506930 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)