BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0111.p (459 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312689|ref|YP_003776781.1| transmembrane sensor kinase tra... 855 0.0 gi|300313819|ref|YP_003777911.1| two component sensor histidine ... 266 3e-72 gi|300309980|ref|YP_003774072.1| two component transmembrane sen... 201 1e-52 gi|300314014|ref|YP_003778106.1| two component sensor histidine ... 185 7e-48 gi|300312679|ref|YP_003776771.1| periplasmic sensor signal trans... 181 9e-47 >gi|300312689|ref|YP_003776781.1| transmembrane sensor kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 459 Score = 855 bits (2209), Expect = 0.0, Method: Compositional matrix adjust. Identities = 439/457 (96%), Positives = 451/457 (98%) Query: 1 MIDSLRLRLLWWLLIPMAVYVGLSTGDAYDNAVSSATLVQDRALLASARMMAGQVGWNDG 60 MIDSLRLRLLWWLLIPMAVYVGLST DAYDNAVSSATLVQDRALLASARM+AGQVGWNDG Sbjct: 1 MIDSLRLRLLWWLLIPMAVYVGLSTSDAYDNAVSSATLVQDRALLASARMIAGQVGWNDG 60 Query: 61 APVVSIPPAALELFASPAQDAVYYQVRMDDETLLAGRPDFPAEPDFAALYPVYTTTTFEG 120 APVVS+PPAALELFASPAQDAVYYQV MDD+TLLAGRPDFPA PDFAALYPVY+TT+F G Sbjct: 61 APVVSVPPAALELFASPAQDAVYYQVLMDDDTLLAGRPDFPAAPDFAALYPVYSTTSFNG 120 Query: 121 KPLRVVSYIRALFDEGTLRMVAVSVGQTMQGRQEMIDDQWHASLRRQIVLLLLAISLVVL 180 KPLRVVSYIRALFDEGTLRMVAVSVGQTMQGRQEMIDDQWHASLRRQIVLLLLAISLVVL Sbjct: 121 KPLRVVSYIRALFDEGTLRMVAVSVGQTMQGRQEMIDDQWHASLRRQIVLLLLAISLVVL 180 Query: 181 GLTMELRPLLALKDELATREPDSLTPLRAGGLQQELRPIVEAINQYIFRLNAQVTVQKRF 240 GLTMELRPLLALKDELATR PDSLTPLRAGGLQQELRPIVEAINQYIFRLNAQVTVQKRF Sbjct: 181 GLTMELRPLLALKDELATRRPDSLTPLRAGGLQQELRPIVEAINQYIFRLNAQVTVQKRF 240 Query: 241 VADAAHQLRTPLAVIDSQIQFARQLDDPARVKQVLASLQEGSRSMTDLTNKLLLLSQAEA 300 VADAAHQLRTPLAVIDSQIQFARQLDDPARV QVLASLQEGSRSMTDLTNKLLLLSQAEA Sbjct: 241 VADAAHQLRTPLAVIDSQIQFARQLDDPARVAQVLASLQEGSRSMTDLTNKLLLLSQAEA 300 Query: 301 SNSSAFPRAPVDLLAVAAMVLEELAGLALKKGIDLGLETTLESAMVNGNEAQLMGLLMNL 360 S+SSAFPRAPVDLLAV+AMVLEEL+GLALKKGIDLGLETTLESAMVNGNEAQLMG+LMNL Sbjct: 301 SDSSAFPRAPVDLLAVSAMVLEELSGLALKKGIDLGLETTLESAMVNGNEAQLMGMLMNL 360 Query: 361 IDNALRYTPEGGKVTVGLEKKRSQIIITVTDNGPGIPAEVRERVFERFYRNAAPDQPGSG 420 IDNALRYTPEGGKVTVGLEKKRSQ+IITV+DNGPGIPAEVRERVFERFYRNAAPDQPGSG Sbjct: 361 IDNALRYTPEGGKVTVGLEKKRSQMIITVSDNGPGIPAEVRERVFERFYRNAAPDQPGSG 420 Query: 421 LGLAIVREIVQASQGAIRLDAPAQGSGLVATVSLPAL 457 LGLAIVREIVQASQG+IRLDAPAQGSGLVATVSLPAL Sbjct: 421 LGLAIVREIVQASQGSIRLDAPAQGSGLVATVSLPAL 457 >gi|300313819|ref|YP_003777911.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 485 Score = 266 bits (680), Expect = 3e-72, Method: Compositional matrix adjust. Identities = 167/470 (35%), Positives = 261/470 (55%), Gaps = 21/470 (4%) Query: 2 IDSLRLRLLWWLLIPMAVYVGLSTGDAYDNAVSSATLVQDRALLASARMMAGQVGWNDGA 61 + SLR +L+ WLLIP+ + V + Y NA+ A L DR+LLAS R +A +V DG Sbjct: 8 LGSLRSQLVRWLLIPLTLLVAVDAVSVYYNALEVADLAYDRSLLASTRALAERVSVVDGH 67 Query: 62 PVVSIPPAALELFASPAQDAVYYQVRMDDETLLAGRPDFPAEP---DFAALYPV---YTT 115 +P AL+ F + +YY+V + L++G D P P + +YP + Sbjct: 68 VTADVPYVALDSFETDNLGRIYYKVTGLNGELVSGYDDLPPVPPDVKRSEVYPALVRFYQ 127 Query: 116 TTFEGKPLRVVSYIRALFDEGTLRMVAVSVGQTMQGRQEMIDDQWHASLRRQIVLLLLAI 175 + +P+R+ + ++ ++D+ + + VG+TM+GR M +L RQ L+ +A Sbjct: 128 ADYHDQPVRIAALLQPVYDDSIRGIALIQVGETMEGRNGMTRKILFDTLWRQGTLVTVAA 187 Query: 176 SLVVLGLTMELRPLLALKDELATREPDSLTPLRAGGLQQELRPIVEAINQYIFRLNAQVT 235 LV + LRPL+ LK E+ +R P L+ + +E+RP+V+A+N Y+ RL A ++ Sbjct: 188 LLVWFAVRFVLRPLMQLKGEVESRAPTDLSGFDTALVHKEMRPLVQAMNGYMGRLQALIS 247 Query: 236 VQKRFVADAAHQLRTPLAVIDSQIQFA-----RQLDDPARVKQVLASLQEGSRSMTDLTN 290 Q+RF+ADA+HQLRTPL V+ +Q + R+ D A ++ ++ + + + L N Sbjct: 248 AQRRFIADASHQLRTPLTVLKTQSELTLRELRREQVDAAELRDLVEGMARTTDAAVHLAN 307 Query: 291 KLLLLSQAEASNSSAFPRAPVDLLAVAAMVLEELAGLALKKGIDLGLETTLESAMVNGNE 350 +LL L++AE + P+ L A V EL+ A+ + I L E + + + N Sbjct: 308 RLLTLARAEHGAAEG-GTMPLSLSDAARQVALELSQQAVSREIGLAFEEEGDVS-IEANA 365 Query: 351 AQLMGLLMNLIDNALRYTPEGGKVTVGLEKKRSQ-----IIITVTDNGPGIPAEVRERVF 405 L L++NL+DNA+RYTP GG VT+ + + S ++ V D+GPGIPA RE+VF Sbjct: 366 LLLHELMVNLLDNAIRYTPAGGHVTLRVRAQSSPGQPTVALLEVEDSGPGIPAAEREKVF 425 Query: 406 ERFYRNAAPDQ---PGSGLGLAIVREIVQASQGAIRLDAPAQGSGLVATV 452 E FYR A G+GLGL+IVREI + ++ LDAPAQGSGLV + Sbjct: 426 EPFYRAGATQHVNPGGTGLGLSIVREIAAMHRASVTLDAPAQGSGLVVRI 475 >gi|300309980|ref|YP_003774072.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 390 Score = 201 bits (511), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 116/307 (37%), Positives = 178/307 (57%), Gaps = 6/307 (1%) Query: 139 RMVAVSVGQTMQGRQEMIDDQWHASLRRQIVLLLLAISLVVLGLTMELRPLLALKDELAT 198 R+ V VG+T++ R ++ ++ + Q ++L +A++LV L+ L PL L+ + Sbjct: 51 RLATVQVGETLEKRAQLANEIIKGVILPQFIILPVALALVWFALSRGLSPLSELQQRIRA 110 Query: 199 REPDSLTPLRAGGLQQELRPIVEAINQYIFRLNAQVTVQKRFVADAAHQLRTPLAVIDSQ 258 R PD L+P+ +G + +E+ P+V ++N + RL+ + +QKRF+ADAAHQ++TPLA + Q Sbjct: 111 RRPDDLSPIDSGQVPEEISPLVRSLNDMLARLSQTIAIQKRFIADAAHQMKTPLAGMRMQ 170 Query: 259 IQFARQLDDPARVKQVLASLQEGSRSMTDLTNKLLLLSQAEASNSSAFPRAPVDLLAVAA 318 + A + DD V++ L L + S + T L N+LL L++AE S + PV+L +A Sbjct: 171 SELALRQDDQVDVRRSLEQLAKSSETATRLVNQLLSLARAENQQSQSGGMIPVELNELAR 230 Query: 319 MVLEELAGLALKKGIDLGLETTLESAMVNGNEAQLMGLLMNLIDNALRYTPEGGKVTVGL 378 V+++ ++ + IDLG E ++ GN L LL NL+DNAL YTP G V + Sbjct: 231 SVVQDWVPMSFTQRIDLGFEQPDHPILITGNPVMLRELLSNLLDNALHYTPHDGNGRVTV 290 Query: 379 EKKRSQ----IIITVTDNGPGIPAEVRERVFERFYRNAAPDQPGS--GLGLAIVREIVQA 432 + + I+ V DNGPGIP R VFERFYR GS GLGLAIVREI Q Sbjct: 291 RARTDEGGGLAILEVEDNGPGIPVAERANVFERFYRILGTPSTGSGLGLGLAIVREIAQQ 350 Query: 433 SQGAIRL 439 + A+ + Sbjct: 351 HEAALEI 357 >gi|300314014|ref|YP_003778106.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 467 Score = 185 bits (469), Expect = 7e-48, Method: Compositional matrix adjust. Identities = 139/466 (29%), Positives = 233/466 (50%), Gaps = 18/466 (3%) Query: 4 SLRLRLLWWLLIPMAVYVGLSTGDAYDNAVSSATLVQDRALLASARMMAGQVGWNDGAPV 63 S+R LL W++ P+A+++ + + Y N + S DR L+AS + + +G Sbjct: 10 SVRAYLLAWIISPIALFIIIDSFSLYRNTLESVNTAYDRMLIASVHSIGDLLRIENGELK 69 Query: 64 VSIPPAALELFASPAQDAVYYQVRMDDETLLAGRPDFPA---EPDFAALYPVYTTT---T 117 S+P AALE++ + + Y++ D G D P+ + D A+YP T Sbjct: 70 ASLPYAALEIYEADYSSRMIYRINGLDGKFFDGDADLPSYKGKADKEAIYPALAHIYEDT 129 Query: 118 FEGKPLRVVSYIRALFDEGTLRMVAVSVGQTMQGRQEMIDDQWHASLRRQIVLLLLAISL 177 + G P+RV + + + V V +TM+ R + + +L RQ LL++ +S+ Sbjct: 130 YNGVPVRVAALFQPVVTNDPRGAALVQVAETMENRSALARKIFWETLIRQAALLVVIVSV 189 Query: 178 VVLGLTMELRPLLALKDELATREPDSLTPLRAGGLQQELRPIVEAINQYIFRLNAQVTVQ 237 + + L+P+ AL+ +L R D L+P+ +EL+P ++A+NQ + RL + Q Sbjct: 190 TLYVVRRALQPVDALRRQLDERPADDLSPVAPPMAPRELQPFIDALNQLMARLRRLLDHQ 249 Query: 238 KRFVADAAHQLRTPLAVIDSQIQFARQLDDPARVKQVLASLQEGSRSMTDLTNKLLLLSQ 297 +RFVA+A+HQLRTPLAV+ +Q+Q + D PA V + + T L N+LL L++ Sbjct: 250 QRFVANASHQLRTPLAVLKTQLQSGLRGDAPAPV--IWQEMSGTVERATTLANQLLSLAK 307 Query: 298 AEASNSSAFPRAPVDLLAVAAMVLEELAGLALKKGIDLGLETTLESAMVNGNEAQLMGLL 357 E + DL +A +L+ L +K +D LE E +V G+ + L+ Sbjct: 308 VEQIRGRG-AQEVCDLGMLARETAVDLSPLIAEKDLDFELEA--EKILVMGHPWMIGELI 364 Query: 358 MNLIDNALRYTPEGGKVTVGLEKKRSQIIITVTDNGPGIPAEVRERVFERFYRNAAPDQP 417 NL+ NA+R+TP G++ + + + + + D+G GI + E VF+ F N + Sbjct: 365 SNLLHNAIRHTPAQGRLGIRITAREDVAELLIWDSGEGIDDQAMENVFKAFSSNVS---S 421 Query: 418 GSGLGLAIVREIVQASQGAI----RLDAPAQGSGLVATVSLPALAG 459 GLGL I EIV + Q I R+ GL A+V + +AG Sbjct: 422 AGGLGLTICGEIVDSMQATISLRNRVGEDGTIEGLNASVHVRVVAG 467 >gi|300312679|ref|YP_003776771.1| periplasmic sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 181 bits (460), Expect = 9e-47, Method: Compositional matrix adjust. Identities = 143/466 (30%), Positives = 229/466 (49%), Gaps = 27/466 (5%) Query: 1 MIDSLRLRLLWWLLIPMAVYVGLSTGDAYDNAVSSATLVQDRALLASARMMAGQVGWNDG 60 M S+R LL WL+ P+ + + G Y A A D+ L+ S + QV Sbjct: 4 MHSSIRRDLLKWLIAPLLLVNLIGAGLTYWLAWLPAQHAFDQNLMDSTWGLYAQVRHRQE 63 Query: 61 APVVSIPPAALELFASPAQDAVYYQVRMDDETLLAGRPDFPAEPDFAAL-YPVYTTTTFE 119 + A ++ S D ++ VR + ++ G FP P P+ Sbjct: 64 RTTAELTQQAEQILRSNHSDTTFFAVRNTEGQIVVGDKGFPQLPASDVFDRPINYDGEMR 123 Query: 120 GKPLRVVSYIRALFDEGTLRMVAVSVGQTMQGRQEMIDDQWHASLRRQIVLL-----LLA 174 G+P+R+ + ++ G ++ V+V +T++ R D+ H ++ ++L + Sbjct: 124 GEPVRIAA-MQVRVKNG---VIWVAVAETIRKR-----DRAHYTILLSFLVLDGALTFAS 174 Query: 175 ISLVVLGLTMELRPLLALKDELATREPDSLTPLRAGGLQQELRPIVEAINQYIFRLNAQV 234 I +V++ + LRPL +L+ L R+ L + G EL+P++ A+N+ + R+N Sbjct: 175 IFVVLVAVRRGLRPLKSLQRNLEQRDHGKLGAIDGRGAPVELQPLIVAMNELMARINKGE 234 Query: 235 TVQKRFVADAAHQLRTPLAVIDSQIQF--ARQLDDPARVKQVLASLQEGSRSMTDLTNKL 292 Q+ F+AD AHQLRTPL + Q++ + D P + LA + M + +L Sbjct: 235 QAQQNFMADVAHQLRTPLTGLKLQLELLHDKHQDQPDTARS-LAMMNSSVERMIRQSRQL 293 Query: 293 LLLSQAEASNSSAFPRAPVDLLAVAAMVLEELAGL---ALKKGIDLGLETTLESAMVNGN 349 L L+++E F ++ L++ +V E + A KK IDLG + L A + G+ Sbjct: 294 LALARSE---PGLFESRKLEELSLDKLVEESVQHFIEEADKKRIDLGFD--LRPARLRGD 348 Query: 350 EAQLMGLLMNLIDNALRYTPEGGKVTVGLEKKRSQIIITVTDNGPGIPAEVRERVFERFY 409 L L+ NLIDNA+RY+P G VTV +++ +++V D+GPGI E RE +F+RFY Sbjct: 349 RFLLSDLIDNLIDNAVRYSPPHGTVTVRCLEEQDATVLSVEDSGPGIAPEHRELIFDRFY 408 Query: 410 RNAAPDQPGSGLGLAIVREIVQASQGAIRLDAPAQGSGLVATVSLP 455 R A GSGLGLAIVREI G I +D QG G + TV P Sbjct: 409 R-ANDKVAGSGLGLAIVREIAHDHGGVISVDCKEQGQGTIFTVRFP 453 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,240,470 Number of extensions: 48803 Number of successful extensions: 342 Number of sequences better than 1.0e-08: 44 Number of HSP's gapped: 272 Number of HSP's successfully gapped: 44 Length of query: 459 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 371 Effective length of database: 1,203,190 Effective search space: 446383490 Effective search space used: 446383490 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)