BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0106.p (345 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312693|ref|YP_003776785.1| D-xylulose reductase [Herbaspir... 635 0.0 gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herb... 165 6e-42 gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase... 89 7e-19 gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspi... 78 9e-16 gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldeh... 75 1e-14 >gi|300312693|ref|YP_003776785.1| D-xylulose reductase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 635 bits (1638), Expect = 0.0, Method: Compositional matrix adjust. Identities = 319/345 (92%), Positives = 331/345 (95%) Query: 1 MQALVLEATRELALRDIDLPQDLGAQDVRIRIHTVGICGSDLHYYTHGRIGPFKVEAPMV 60 MQALVLEATREL LR+IDLPQ +GAQDVRIRIHTVGICGSDLHYYTHG IGPFKVEAPMV Sbjct: 1 MQALVLEATRELKLREIDLPQQMGAQDVRIRIHTVGICGSDLHYYTHGSIGPFKVEAPMV 60 Query: 61 LGHEASGTIIEVGSGVTQLKVGDRVCMEPGIPRLDSPATLRGLYNLDPSVRFWATPPIHG 120 LGHEASGT+IEVGS V+ LKVGDRVCMEPGIPRLDSPATLRG+YNLDP+VRFWATPPIHG Sbjct: 61 LGHEASGTVIEVGSAVSHLKVGDRVCMEPGIPRLDSPATLRGMYNLDPAVRFWATPPIHG 120 Query: 121 CLTGSVVHPAAFTYRLPDNVSFAEGAIVEPLSIGLQAATKARMKPGDTAVVIGAGTIGAM 180 CLTGSVVHPAAFTYRLPDNVSFAEGAIVEPLSIGLQAATKARMKPGDTAVVIGAGTIGAM Sbjct: 121 CLTGSVVHPAAFTYRLPDNVSFAEGAIVEPLSIGLQAATKARMKPGDTAVVIGAGTIGAM 180 Query: 181 TALAALAGGAARVILADVVAEKLAHFANNPAVITVDVTQQALTDVVQQVTEGWGADVVFE 240 TALAALAGGAARVILADVVAEKLAHFA+NPAVITVDVT++ LTDVV+Q T+GWGADVVFE Sbjct: 181 TALAALAGGAARVILADVVAEKLAHFADNPAVITVDVTRETLTDVVRQATDGWGADVVFE 240 Query: 241 ASGHAGVYQTLLDLVCPGGCAVLVGMPPEPVGLDVVTMQTKEVRLESVFRYANIFPRALA 300 ASGHAGVYQTLLDLVCPGGCAVLVGMPP PV LDVV MQTKEVRLESVFRYANIFPRALA Sbjct: 241 ASGHAGVYQTLLDLVCPGGCAVLVGMPPAPVALDVVAMQTKEVRLESVFRYANIFPRALA 300 Query: 301 LLSSGMIDVKPFISRSFPFSQGIEAFEEAARGRPQDVKIQIEMEG 345 L+SSGMIDVKPFISR FPFSQ I AFEEAA GRPQDVKIQIEMEG Sbjct: 301 LISSGMIDVKPFISRKFPFSQSIRAFEEAASGRPQDVKIQIEMEG 345 >gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 165 bits (417), Expect = 6e-42, Method: Compositional matrix adjust. Identities = 111/347 (31%), Positives = 175/347 (50%), Gaps = 8/347 (2%) Query: 2 QALVLEATRELALRDIDLPQDLGAQDVRIRIHTVGICGSDLHYYTHGRIGPFKVEAPMVL 61 Q LV+ A +L ++ +D L Q +++++ GICGSDLHYY HG G +++ PMVL Sbjct: 3 QGLVIHAPHDLRIQALDTGA-LQPQQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMVL 61 Query: 62 GHEASGTIIEVGSGVTQLKVGDRVCMEPGIPRLDSPATLRGLYNLDPSVRFWA----TPP 117 GHE SG I EVG+ VT + G R+ + P P G N +RF+ TP Sbjct: 62 GHEVSGQIAEVGTAVTDIAPGTRIAISPSRPCGACKYCQEGKQNHCLDMRFYGSAMRTPH 121 Query: 118 IHGCLTGSVVHPAAFTYRLPDNVSFAEGAIVEPLSIGLQAATKARMKPGDTAVVIGAGTI 177 + G ++ + + + D+VS E A+ EPLS+ L A +A G +V G G I Sbjct: 122 VQGAFRQEIIIERSQAHVVADHVSDGEAAMAEPLSVALHAVRRAGPLVGKRVLVTGCGPI 181 Query: 178 GAMTALAALAGGAARVILADVVAEKLAHFANNPAVITVDVTQQALTDVVQQVTEGWGADV 237 GA+ +AA GA V++ DV + L A +++ + +G DV Sbjct: 182 GALVVVAARRAGALEVVVTDVASMPLQAALQVGADSAINMAETPAALKAYAADKGT-FDV 240 Query: 238 VFEASGHAGVYQTLLDLVCPGGCAVLVGMPPEPVGLDVVTMQTKEVRLESVFRYANIFPR 297 +FEASG+ L+ + P V VG+ + + + KE+ FR+ F Sbjct: 241 LFEASGNERALVGALEALRPQAIIVQVGLGGD-IQFPANLLVGKELEWRGAFRFHEEFAM 299 Query: 298 ALALLSSGMIDVKPFISRSFPFSQGIEAFEEAARGRPQDVKIQIEME 344 A+ L++ ++DVKP I+ ++P +Q +EAFE A R + +K+QI + Sbjct: 300 AVELMNRKLVDVKPLITATYPIAQAVEAFEIAG-DRSRMMKVQISFD 345 >gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 374 Score = 88.6 bits (218), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 99/346 (28%), Positives = 142/346 (41%), Gaps = 46/346 (13%) Query: 16 DIDLPQDLGAQDVRIRIHTVGICGSDLHYYTHGRIGPFKVEAPMVLGHEASGTIIEVGSG 75 D+D P G +V +R+ G+C SDL T R P PM LGHEAS ++EVGSG Sbjct: 29 DLDPP---GPGEVLVRMKAAGLCHSDLSVITGVRPRPV----PMALGHEASAEVVEVGSG 81 Query: 76 VTQLKVGDRV-------------CMEPGIPRLDSPA----TLRGLYNLDPSVRFWATPPI 118 V L+ GD V CME G P L P T+ L + +R T P+ Sbjct: 82 VNDLRAGDLVVLVFVPSCGHCLPCME-GRPALCEPGAATNTVGELLSGQRRLRD-KTQPV 139 Query: 119 HGCLTGSVVHPAAFTYRLPDNVSFAEGAIVEPLSIGL----------QAATKARMKPGDT 168 H L S A R A ++P+ L A A +K G T Sbjct: 140 HHHLGVSAFADHAVVSR---RSCVKIEADIDPVQAALFGCAVLTGVGAAVNTAEVKAGTT 196 Query: 169 AVVIGAGTIGAMTALAALAGGAARVILADVVAEKLAHFANNPAVITVDVTQQALTDVVQQ 228 A V+G G +G L ALA GA VI D+ KLA + A TV+ + V+ Sbjct: 197 AAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLALARSLGATATVNARDPDAVEKVRA 256 Query: 229 VTEGWGADVVFEASGHAGVYQTLLDLVCPGGCAVLVGMPP--EPVGLDVVTMQTKEVRLE 286 +T G G D FE +G + + GG V G+P + L V++ +E ++ Sbjct: 257 LTRG-GVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGLPAPGQHWALQQVSLVAEERTVK 315 Query: 287 SVFRYANI----FPRALALLSSGMIDVKPFISRSFPFSQGIEAFEE 328 + + + PR + L +G + V + + F+ Sbjct: 316 GSYIGSCVPVRDIPRYIGLFQAGKLPVDRLMGERLALADINRGFDR 361 >gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 341 Score = 78.2 bits (191), Expect = 9e-16, Method: Compositional matrix adjust. Identities = 82/291 (28%), Positives = 122/291 (41%), Gaps = 25/291 (8%) Query: 3 ALVLEATRELALRDIDLPQDLGAQDVRIRIHTVGICGSDLHYYTHGRIGPFKVEAPMVLG 62 A+V E + L++ + +P Q + ++ G+C +DLH HG P K P + G Sbjct: 8 AVVREFGKPLSIEQVPVPTPAPGQ-ILVKFEASGVCHTDLHA-AHGDW-PVKPTPPFIPG 64 Query: 63 HEASGTIIEVGSGVTQLKVGDRVCMEPGIPRLDSPATLRGLYNLDPSVRFWATPPIHGCL 122 HE +G + VG+GV +K GDRV G+P L + P W T Sbjct: 65 HEGTGYVAAVGAGVKHVKEGDRV----GVPWLHTACGC-----CSPCRTGWETLCAEQQN 115 Query: 123 TGSVVHPA---------AFTYRLPDNVSFAEGAIVEPLSIGLQAATK-ARMKPGDTAVVI 172 TG V+ + F LPDN+ F A V + + K ++PG+ V+ Sbjct: 116 TGYSVNGSFAEYGLADPKFVGHLPDNLDFGPAAPVLCAGVTVYKGLKETEVRPGEWVVIS 175 Query: 173 GAGTIGAMTALAALAGGAARVILADVVAEKLAHFANNPAVITVDVTQQALTDVVQQVTEG 232 G G +G M A A G V+ AD+ +KLA A +TVD VQ++ G Sbjct: 176 GIGGLGHMAVQYAKAMG-MHVVAADIHEDKLALAKKLGADLTVDGRNHNAVAEVQRIIGG 234 Query: 233 WGADVVFEASGHAGVYQTLLDLVCPGGCAVLVGMPPEPVGLDVVTMQTKEV 283 +V S A + + G LVG+PP + L V K + Sbjct: 235 AHGALVTAVSPKA--MEQAFGFLRARGTMALVGLPPGDISLPVFNTVLKRI 283 >gi|300309893|ref|YP_003773985.1| glutathione-dependent formaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 74.7 bits (182), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 73/267 (27%), Positives = 115/267 (43%), Gaps = 27/267 (10%) Query: 1 MQALVLEATRELALRDIDLPQDLGAQDVRIRIHTVGICGSDLHYYTHGRIGPFKVEAPMV 60 M+AL+ + ++ + + P A DV +++ ICGSDLH Y G+I K A + Sbjct: 1 MRALLYHSAHDVRVETVPDPIIQEADDVILKVTATAICGSDLHLY-RGKIPATK--AGDI 57 Query: 61 LGHEASGTIIEVGSGVTQLKVGDRV-------------CMEPGIPRLDSPATLRGLYNLD 107 LGHE G ++E G + +LK GDRV C + ++ RG Sbjct: 58 LGHEFMGVVVEAGPAIKRLKKGDRVVVPFVIACGQCFFCDQHLYAACETTNPDRGALMNA 117 Query: 108 PSVRFWATPPIHGCLTGSVVHPAAFTYRLPD--------NVSFAEGAIV---EPLSIGLQ 156 +R A + L G + A R+P + + A+ ++ + L G Q Sbjct: 118 KGIRSGAAMFGYSHLYGGMPGGQAEYVRVPKADVGPIRIDSALADEQVLFLSDILPTGYQ 177 Query: 157 AATKARMKPGDTAVVIGAGTIGAMTALAALAGGAARVILADVVAEKLAHFANNPAVITVD 216 A A ++ G + + GAG +G M A +A GA R+ + D A +L VI V+ Sbjct: 178 AVKNAGVREGSSVAIYGAGPVGQMAAASARMLGAERIFMVDHHAYRLRFAQEQYDVIPVN 237 Query: 217 VTQQALTDVVQQVTEGWGADVVFEASG 243 Q + + T G G D V +A G Sbjct: 238 FDQVDPAEYIIANTAGRGVDGVIDAVG 264 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.138 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,047,793 Number of extensions: 44649 Number of successful extensions: 176 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 171 Number of HSP's successfully gapped: 5 Length of query: 345 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 259 Effective length of database: 1,212,660 Effective search space: 314078940 Effective search space used: 314078940 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)