BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0100.p (160 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312702|ref|YP_003776794.1| VGR-related protein [Herbaspiri... 278 1e-76 gi|300310098|ref|YP_003774190.1| VGR-related protein [Herbaspiri... 74 7e-15 gi|300313606|ref|YP_003777698.1| VGR-related type VI secretion s... 70 1e-13 gi|300310274|ref|YP_003774366.1| VGR-related protein [Herbaspiri... 61 5e-11 gi|300310071|ref|YP_003774163.1| VGR-related protein [Herbaspiri... 61 5e-11 >gi|300312702|ref|YP_003776794.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 824 Score = 278 bits (712), Expect = 1e-76, Method: Compositional matrix adjust. Identities = 133/160 (83%), Positives = 142/160 (88%) Query: 1 MHSRTTGWDSLTRQRQHQRILQLHFVNDDAPDVELLINRLDASEALSRDYAYTLELLSDD 60 MHS TT WD LTRQRQH RILQL F+NDDAPD ELLINRL+ASEALSRDY YTLELLSDD Sbjct: 1 MHSHTTRWDKLTRQRQHNRILQLFFINDDAPDAELLINRLEASEALSRDYVYTLELLSDD 60 Query: 61 ACIPLEALMGKLLRVALVRADGTQRHFTGYVERFSFLRTDGGIAFYEARLVPWMAFLAAR 120 A IPL+A+MGKLLRVAL RADGTQRHFTGYVERF+FLR+DGGIAFYEARLVPW+AFLA+R Sbjct: 61 AHIPLDAMMGKLLRVALTRADGTQRHFTGYVERFAFLRSDGGIAFYEARLVPWLAFLASR 120 Query: 121 INNRIFHQRNLEGLSAEIFSAYPSHARWDCQLRHDDPSYT 160 NNRIFH +LEGLSA IF AYP HARWDCQLRH DP T Sbjct: 121 HNNRIFHHLSLEGLSAAIFRAYPMHARWDCQLRHGDPVRT 160 >gi|300310098|ref|YP_003774190.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 982 Score = 73.6 bits (179), Expect = 7e-15, Method: Compositional matrix adjust. Identities = 39/123 (31%), Positives = 69/123 (56%) Query: 26 VNDDAPDVELLINRLDASEALSRDYAYTLELLSDDACIPLEALMGKLLRVALVRADGTQR 85 + D AP ELL+ ASE L + +LS DA + L++L+G+ + +DG++ Sbjct: 22 LGDAAPAGELLVEAFAASEGLHAVGLREVIVLSLDAGLRLKSLIGQRATLVTTLSDGSRT 81 Query: 86 HFTGYVERFSFLRTDGGIAFYEARLVPWMAFLAARINNRIFHQRNLEGLSAEIFSAYPSH 145 F+G V + L ++GG+A Y R+VPW+ L+ N+R++ + + + ++F+ Y H Sbjct: 82 QFSGLVNEAAKLGSEGGLARYRLRIVPWVWLLSQSHNSRVWQDKTVIDIVEDVFADYAPH 141 Query: 146 ARW 148 A W Sbjct: 142 AAW 144 >gi|300313606|ref|YP_003777698.1| VGR-related type VI secretion system protein [Herbaspirillum seropedicae SmR1] Length = 886 Score = 69.7 bits (169), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 46/134 (34%), Positives = 73/134 (54%), Gaps = 3/134 (2%) Query: 10 SLTRQRQHQRILQLHFVNDDAPDVELLINRLDASEALSRDYAYTLELLSDDACIPLEALM 69 S R QH+R+LQL + PD+ LL RL A+E L+ Y T++LL+ I LE L+ Sbjct: 12 SQARITQHRRLLQLD--SSLGPDI-LLPQRLVATERLNDGYEVTIDLLATHTGIALEQLI 68 Query: 70 GKLLRVALVRADGTQRHFTGYVERFSFLRTDGGIAFYEARLVPWMAFLAARINNRIFHQR 129 + + + + + DG GYV L +DG + F + L PW+ FL R + RI+ + Sbjct: 69 AQPVTLWIRQHDGDTLPLHGYVHTAKRLGSDGELDFCQLSLSPWLHFLRFRKDARIWQDK 128 Query: 130 NLEGLSAEIFSAYP 143 E + A++F+ +P Sbjct: 129 RTEDILADVFNNHP 142 >gi|300310274|ref|YP_003774366.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 928 Score = 60.8 bits (146), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 41/124 (33%), Positives = 65/124 (52%), Gaps = 3/124 (2%) Query: 16 QHQRILQLHFVNDDAPDVELLINRLDASEALSRDYAYTLELLSDDACIPLEALMGKLLRV 75 Q +RIL+L V PDV LL R+ SE L R Y YT++L+S I L+ L+ + + + Sbjct: 30 QEKRILKLDSVL--GPDV-LLPQRMVGSERLGRGYDYTIDLVSTRDDIELKKLIAQPVTL 86 Query: 76 ALVRADGTQRHFTGYVERFSFLRTDGGIAFYEARLVPWMAFLAARINNRIFHQRNLEGLS 135 L + GYV L +DG +A ++ + PW+ FL R + RI+ + + Sbjct: 87 WLQQDGSDYAPLHGYVHTARRLGSDGQLASFQIGMAPWLHFLKYRKDARIWQDSKADDIL 146 Query: 136 AEIF 139 A++F Sbjct: 147 ADVF 150 >gi|300310071|ref|YP_003774163.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 954 Score = 60.8 bits (146), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 33/128 (25%), Positives = 67/128 (52%) Query: 16 QHQRILQLHFVNDDAPDVELLINRLDASEALSRDYAYTLELLSDDACIPLEALMGKLLRV 75 Q+ R++ + L + R + E+++ ++ + ++ LS + L+ +G + + Sbjct: 23 QNARLITMTTAQSAGLPETLAVERFEGEESVNDNFRFDIDALSISTDLDLKQFIGAEISL 82 Query: 76 ALVRADGTQRHFTGYVERFSFLRTDGGIAFYEARLVPWMAFLAARINNRIFHQRNLEGLS 135 L++ADG R + GY + S+L DGG+A Y RL ++AFL R ++ +F + ++ Sbjct: 83 RLLQADGGTRAWHGYCTQASWLGADGGLARYRLRLESFLAFLDRRHDSFLFQDMTVTDIA 142 Query: 136 AEIFSAYP 143 A + YP Sbjct: 143 AAVLKEYP 150 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.138 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 454,314 Number of extensions: 16204 Number of successful extensions: 58 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 58 Number of HSP's successfully gapped: 5 Length of query: 160 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 82 Effective length of database: 1,250,540 Effective search space: 102544280 Effective search space used: 102544280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 127 (53.5 bits)