BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0099.p (440 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312702|ref|YP_003776794.1| VGR-related protein [Herbaspiri... 650 0.0 gi|300310274|ref|YP_003774366.1| VGR-related protein [Herbaspiri... 172 7e-44 gi|300313606|ref|YP_003777698.1| VGR-related type VI secretion s... 164 2e-41 gi|300309366|ref|YP_003773458.1| VGR-related protein [Herbaspiri... 130 2e-31 gi|300309592|ref|YP_003773684.1| VGR-related protein [Herbaspiri... 126 4e-30 >gi|300312702|ref|YP_003776794.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 824 Score = 650 bits (1677), Expect = 0.0, Method: Compositional matrix adjust. Identities = 305/341 (89%), Positives = 320/341 (93%) Query: 1 MFQFDESDSNLLHRRWEDAGWHYHYEHQSDGHQLRLSDDSTYANPIDGGGQVPFQHHGGA 60 MFQFDESDSNLLHRRWEDAGWHYHYEHQ+DGHQLRLSDDSTYA PIDG GQVPFQ HGGA Sbjct: 162 MFQFDESDSNLLHRRWEDAGWHYHYEHQADGHQLRLSDDSTYAKPIDGTGQVPFQRHGGA 221 Query: 61 IEEDGMAQWSMVRQFQPSSTRLSSYDFKTARPQQVDVPTLNKQGAVLPVEHYEYTGAYGF 120 IEEDGMAQWS VRQFQPSSTRLSSYDFK ARPQ+V+VPTLNKQGAVL VEHYEY GAYGF Sbjct: 222 IEEDGMAQWSAVRQFQPSSTRLSSYDFKAARPQEVEVPTLNKQGAVLQVEHYEYAGAYGF 281 Query: 121 SNLEAGDRQSRVRMEEFEAAAQLFEGIGNCRFLQPGRTFRLTGHFSAPATSHDHDRDPEL 180 +N + GDRQSR+RMEEFEAAAQ+FEG GNCRFLQPGRTFRLTGHFS A SHDHDRDPEL Sbjct: 282 ANRDDGDRQSRLRMEEFEAAAQVFEGTGNCRFLQPGRTFRLTGHFSGSAASHDHDRDPEL 341 Query: 181 LIVSVEHSATNNYLQDSETPPTYTNRVRCVPKRIPWRPGRSYYSRSTALHGIQTATVVGP 240 LI+SVEHSATNNYLQDSETPP Y NRVRCV KRIPWRPGRSYYS+ TAL GIQTATVVGP Sbjct: 342 LIISVEHSATNNYLQDSETPPFYANRVRCVRKRIPWRPGRSYYSQPTALLGIQTATVVGP 401 Query: 241 SGENLHVDEYGRVKVQFHWDLLGRNDERSSAWLRVASSWTGAEQGLVAVPRIGQLVIVQW 300 +GENLHVDEYGRVKVQFHWD +GRNDERSSAWLRVASSW+G EQGLV+VPRIGQLVIVQW Sbjct: 402 AGENLHVDEYGRVKVQFHWDQIGRNDERSSAWLRVASSWSGGEQGLVSVPRIGQLVIVQW 461 Query: 301 VGGHPDRPIITGSVVNQLNMPPWSLPSQSALSGMRSRELMP 341 +GGHPDRPIITGSVVNQLNMPPW+LPSQSALSGMRSREL P Sbjct: 462 LGGHPDRPIITGSVVNQLNMPPWTLPSQSALSGMRSRELAP 502 >gi|300310274|ref|YP_003774366.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 928 Score = 172 bits (435), Expect = 7e-44, Method: Compositional matrix adjust. Identities = 119/371 (32%), Positives = 189/371 (50%), Gaps = 49/371 (13%) Query: 6 ESDSNLLHRRWEDAGWHYHYEHQSDG--HQLRLSDDSTYANPIDGGGQVPFQHHGGAIEE 63 E+D + + R E+ GW+ ++E + DG H L ++D++ A P+ Q+ F G E Sbjct: 178 ETDWHFVQRLMEEEGWYAYHEQKPDGSGHVLVITDNTDQAKPL-APEQLAFHGAGTQDEV 236 Query: 64 DGMAQWSMVRQFQPSSTRLSSY--DFKTARPQQVDVPTLNKQGAVLP--VEHYEYTGAYG 119 + + WS R +STRL++ D+K+ Q+ + + LP +E YEY+G Y Sbjct: 237 NRILHWSASRGL--ASTRLATRTDDYKSPGDQKQTQERVLPEHGALPGELEVYEYSGPYS 294 Query: 120 FSNLEAGDRQSRVRMEEFEAAAQLFEGIGNCRFLQPGRTFRLTGHFSAPATSHDHDRDPE 179 ++ + GDRQ+R+R+E++E++ + F G+ R L GR F LT H PA D +D + Sbjct: 295 YAKQDDGDRQARLRVEQWESSMKRFMGVSGARTLAAGRWFTLTDH---PAHRGDSKQDSQ 351 Query: 180 LLIVSVEHSATNNYLQDSETPPTY-------------------------------TNRVR 208 ++++VE NN L S T + NR Sbjct: 352 FMLIAVEWFIENN-LPLSGTARDFPGSLKPQLDAFKASMGRSNDDAGGNARTGHCFNRFE 410 Query: 209 CVPKRIPWRPGRSYYSRSTALHGIQTATVVGPSGENLHVDEYGRVKVQFHWDLLGRNDER 268 +++P+R S + + + QTA VVGP GE + D+ RVKVQF WD + DE+ Sbjct: 411 VQRRKLPYR---SPFDHTKPVMHPQTAVVVGPQGEEVFTDKLNRVKVQFRWDRINPGDEK 467 Query: 269 SSAWLRVASSWTGAEQGLVAVPRIGQLVIVQWVGGHPDRPIITGSVVNQLNMPPWSLPSQ 328 +S W+RV+ G G V VPR+GQ +I+ ++ G PDRP+ TG + NQ P W + Sbjct: 468 ASCWVRVSYPNAGENWGEVKVPRVGQELIISFLNGDPDRPVATGRLFNQEQPPQWH--TD 525 Query: 329 SALSGMRSREL 339 LSG++S+E Sbjct: 526 GRLSGLKSKEF 536 >gi|300313606|ref|YP_003777698.1| VGR-related type VI secretion system protein [Herbaspirillum seropedicae SmR1] Length = 886 Score = 164 bits (414), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 120/371 (32%), Positives = 185/371 (49%), Gaps = 48/371 (12%) Query: 6 ESDSNLLHRRWEDAGWHYHYEHQSDG--HQLRLSDDSTYANPIDGGGQVPFQHHGGA--I 61 E+D + + R E+ GW H+E + DG H L ++D+ T+ P G Q HG A Sbjct: 166 ETDWHFVQRLMEEEGWFAHHEQREDGSGHVLVITDN-TWNLP--GLPQQGIAFHGAAPRD 222 Query: 62 EEDGMAQWSMVRQFQPSSTRLSSYDFKTARPQQVDVPTLNKQGAVLP--VEHYEYTGAYG 119 E D + WS R R S D+K+ ++ + + LP +E YEYTGAY Sbjct: 223 ELDKILHWSASRTLSSKGWRGRSDDYKSPGMKKEAEEQVMPEYGQLPAQLEMYEYTGAYS 282 Query: 120 FSNLEAGDRQSRVRMEEFEAAAQLFEGIGNCRFLQPGRTFRLTGHFSAPATSHDHDRDPE 179 F E G+RQ+ +++E++E++ Q + + R L GR FRL H P + DR+ + Sbjct: 283 FRLQEQGNRQADLQVEQWESSMQRYSAVSGVRNLACGRWFRLEEH---PQHRAEPDRERQ 339 Query: 180 LLIVSVEHSATNNY---------------------------LQDSETPPTYT----NRVR 208 +I++++ NN Q +ET +T NR Sbjct: 340 FVILAIDWFVENNLPLSHQALDFPGSLKGALMAFKESIRRERQVAETDNEHTGHCFNRFE 399 Query: 209 CVPKRIPWRPGRSYYSRSTALHGIQTATVVGPSGENLHVDEYGRVKVQFHWDLLGRNDER 268 +++P+RP R++ +R L QTA VVGP+ E ++ D R+KVQF WD + +E Sbjct: 400 VQRRKLPFRPARAH-ARPVMLP--QTAIVVGPASEEVYTDHLDRIKVQFRWDRINPGNEA 456 Query: 269 SSAWLRVASSWTGAEQGLVAVPRIGQLVIVQWVGGHPDRPIITGSVVNQLNMPPWSLPSQ 328 +S W+RV+ G G + VPRIGQ VIV ++ G PDRP++TG + N P W + Sbjct: 457 ASCWVRVSYPNAGQYWGAIQVPRIGQEVIVSFLNGDPDRPVVTGRLFNAEQRPQWH--TN 514 Query: 329 SALSGMRSREL 339 LSG++S+E Sbjct: 515 GRLSGVKSKEF 525 >gi|300309366|ref|YP_003773458.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 913 Score = 130 bits (328), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 108/399 (27%), Positives = 172/399 (43%), Gaps = 70/399 (17%) Query: 1 MFQFDESDSNLLHRRWEDAGWHYHYE-------HQSDG--HQLRLSDDSTYANPIDGGGQ 51 + Q +ESD++ L R W+ G + HQ+ H L L DD+ P + G Sbjct: 170 IMQHNESDADFLRRLWKRRGISWFIRPGEPPLPHQAGSPRHTLVLFDDAVQL-PRNAAGA 228 Query: 52 VPFQHHGGAIEEDGMAQWSMVRQFQPSSTRLSSYDFKTARPQQVDVPTLNKQGAV----- 106 V ++ EEDG+ +W R+ + S ++D P + V T+ +QG + Sbjct: 229 VSYRQDHAPAEEDGIVRWLGTRRLRAGSVSRHAWDHAQDGPMRSQVNTVAEQGELGDHFA 288 Query: 107 LPVEHYEYTGAYGFSNLEAGDRQSRVRMEEFEAAAQLFEGIGNCRFLQPGRTFRLTGHFS 166 ++ Y + + E + + RM+ F+ R L+ G + H Sbjct: 289 RLLDDYFIEAPHIADDREDFHQIATARMQRLAQETAEFQADSTIRALRVGEWIGVKDH-- 346 Query: 167 APATSHDHDRDPELLIVSVEHSATNNYLQDSETPPTYTNRVRCVPKR--------IPWR- 217 P + E +I +E A NN+ P + VR V + P R Sbjct: 347 -PELDRRAAHEREFVITRLEVYAENNF------PASLDEHVRLVARHHDSSLEDLAPLRR 399 Query: 218 ----PGRSYYSRSTALHG------------------IQTATVVGPSGENLHVDEYGRVKV 255 GR Y +R T + +Q A VVGP+GE L+ D GR+K+ Sbjct: 400 RCLAQGRRYLNRFTCVRRGTPLVAAFDPRVDLPAVRVQNAHVVGPAGEELYCDALGRIKL 459 Query: 256 QFHWDLL----------GRNDERSSAWLRVASSWTGAEQGLVAVPRIGQLVIVQWVGGHP 305 +FH + ER SAW+RVAS W G + G +++PR+G V+V ++GG P Sbjct: 460 RFHGTRMQDHQHAGGAGASGSERDSAWVRVASHWAGRQWGAISLPRVGDEVLVDFLGGDP 519 Query: 306 DRPIITGSVVNQLNMPPW-----SLPSQSALSGMRSREL 339 D+PI+ G V N ++PP SLP L+G++S+E+ Sbjct: 520 DKPIVVGRVYNGQSLPPAFSRVGSLPGNRFLAGIKSKEI 558 >gi|300309592|ref|YP_003773684.1| VGR-related protein [Herbaspirillum seropedicae SmR1] Length = 966 Score = 126 bits (316), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 118/415 (28%), Positives = 173/415 (41%), Gaps = 86/415 (20%) Query: 3 QFDESDSNLLHR--RWEDAGWHYHYEHQSDG-----------HQLRLSDDSTYANPIDGG 49 QF+ESD++ + R R E W+ Q+D H L D P Sbjct: 177 QFNESDAHFIRRLCRREGIAWYVASGQQADETAIATHADIPIHTLVFCDQPALL-PRAAA 235 Query: 50 GQVPFQHHGGAIEEDGMAQWSMVRQFQPSSTRLSSYDFKTARPQQVDVPTLNKQGAVLPV 109 G V + G E D ++ WS R P S S+D+K R ++ ++ QG Sbjct: 236 GTVRYHRDGATEERDAISAWSRQRSLVPGSVWRRSWDYKAVRAEEASSHSIVDQG----- 290 Query: 110 EHYEYTGAYGFSNL---------EAGDRQ------SRVRMEEFEAAAQLFEGIGNCRFLQ 154 Y +N+ A D Q + RM EA ++ F+ G R L Sbjct: 291 -----DAGYKLANILRDGLIEAPHAADSQNDHLRLADTRMLAHEARSESFDASGTVRDLA 345 Query: 155 PGRTFRLTGHFSAPATSHDHDRDPELLIVSVEHSATNNY---LQD--------------- 196 G F L GH P E L+ S++ A NN+ LQD Sbjct: 346 VGHWFLLDGH---PDMRGQKPAQREFLVTSLQQQAENNFPTVLQDAIRRLAPASPWKNLA 402 Query: 197 -SETPPTYTNRVRCVPKRIPWRPGRSYYSRSTALHGIQTATVVGPSGENLHVDEYGRVKV 255 S Y N++ C + P P +H I TA VVGPS E ++ DE GR+KV Sbjct: 403 HSAADLRYRNQLTCRRRTAPLAPHYDPRLHLPPVHPI-TALVVGPSKEEVYCDELGRIKV 461 Query: 256 QF---------HWDLLGRN-DERSSAWLRVASSWTGAEQGLVAVPRIGQLVIVQWVGGHP 305 Q H G + E+ SA++RV+S+W GA G AVPR+G V++ ++GG P Sbjct: 462 QLQGLHPEDHAHAQGAGASGHEQDSAFVRVSSAWAGAGYGHDAVPRVGMEVLIDFLGGDP 521 Query: 306 DRPIITGSVVNQLNMPP-----WSLPSQSALSGMRSREL---------MPDXPGK 346 D+ + G + N +N+P SLP LSG++++E+ + D PG+ Sbjct: 522 DKMFVAGVLHNGINLPARFSHSGSLPGNRFLSGIKTKEIGGARYNQLRLDDSPGQ 576 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,582,027 Number of extensions: 68945 Number of successful extensions: 174 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 152 Number of HSP's successfully gapped: 13 Length of query: 440 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 352 Effective length of database: 1,203,190 Effective search space: 423522880 Effective search space used: 423522880 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)