BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0082.p (260 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312720|ref|YP_003776812.1| enoyl-[acyl-carrier-protein] re... 520 e-149 gi|300311721|ref|YP_003775813.1| NADH-dependent enoyl-[acyl-carr... 203 9e-54 gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herb... 79 3e-16 gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogena... 61 1e-10 gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydroge... 56 4e-09 >gi|300312720|ref|YP_003776812.1| enoyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 520 bits (1339), Expect = e-149, Method: Compositional matrix adjust. Identities = 258/260 (99%), Positives = 260/260 (100%) Query: 1 MAFLQGKKILITGLLSNRSIAYGIAQACKREGAELAFTYVGERFKDRITKFAEEFDSKLI 60 MAFLQGKKILITGLLSNRSIAYGIAQACKREGAELAFTYVGERFKDRITKFAEEFDSKL+ Sbjct: 1 MAFLQGKKILITGLLSNRSIAYGIAQACKREGAELAFTYVGERFKDRITKFAEEFDSKLV 60 Query: 61 FDCDVGSDEQINALFADLGKSWTQLDGLVHAIGFAPSEAIAGDFLEGFSREGFKIAHDIS 120 FDCDVGSDEQINALFADLGKSW+QLDGLVHAIGFAPSEAIAGDFLEGFSREGFKIAHDIS Sbjct: 61 FDCDVGSDEQINALFADLGKSWSQLDGLVHAIGFAPSEAIAGDFLEGFSREGFKIAHDIS 120 Query: 121 AYSFPAMAKAAMPLLSSNAALLTLTYLGSERVVPNYNTMGLAKASLEASVRYLAESLGPK 180 AYSFPAMAKAAMPLLSSNAALLTLTYLGSERVVPNYNTMGLAKASLEASVRYLAESLGPK Sbjct: 121 AYSFPAMAKAAMPLLSSNAALLTLTYLGSERVVPNYNTMGLAKASLEASVRYLAESLGPK 180 Query: 181 GVRVNGVSAGPIKTLAASGIKGFGKILGAVAATAPLRRNVTIEDVGNASAFLLSDLASGI 240 GVRVNGVSAGPIKTLAASGIKGFGKILGAVAATAPLRRNVTIEDVGNASAFLLSDLASGI Sbjct: 181 GVRVNGVSAGPIKTLAASGIKGFGKILGAVAATAPLRRNVTIEDVGNASAFLLSDLASGI 240 Query: 241 TGEITYVDGGFSRVVGGMAE 260 TGEITYVDGGFSRVVGGMAE Sbjct: 241 TGEITYVDGGFSRVVGGMAE 260 >gi|300311721|ref|YP_003775813.1| NADH-dependent enoyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 255 Score = 203 bits (517), Expect = 9e-54, Method: Compositional matrix adjust. Identities = 106/251 (42%), Positives = 157/251 (62%), Gaps = 1/251 (0%) Query: 4 LQGKKILITGLLSNRSIAYGIAQACKREGAELAFTYVGERFKDRITKFAEEFDSKLIFDC 63 L+GK+ LI G+ SIA+G A+A + GA+LA T++ E + + A++ + ++ Sbjct: 5 LEGKRGLIVGIADKHSIAWGCARALRAAGADLAVTWLNESARRYVEPLADQVQASIMCGL 64 Query: 64 DVGSDEQINALFADLGKSWTQLDGLVHAIGFAPSEAIAGDFLEGFSREGFKIAHDISAYS 123 DV ++ +F + W LD ++H+I FAP+ + G + S +GF +A D+S +S Sbjct: 65 DVTHPGEMEQVFDLIRTHWGSLDFVLHSIAFAPAPDLHGR-VTNCSSQGFSLAMDVSCHS 123 Query: 124 FPAMAKAAMPLLSSNAALLTLTYLGSERVVPNYNTMGLAKASLEASVRYLAESLGPKGVR 183 F MA+ A PL+ + +LLT++Y+G+E VV NY MG KA+LE++VRYLA LGP G+R Sbjct: 124 FIRMARLAEPLMKNGGSLLTMSYIGAEEVVVNYGVMGPVKAALESAVRYLAMELGPAGIR 183 Query: 184 VNGVSAGPIKTLAASGIKGFGKILGAVAATAPLRRNVTIEDVGNASAFLLSDLASGITGE 243 VN VS GP+ T AASG+ F + L A +PLRR I+DVG+ +FL+SD AS ITG Sbjct: 184 VNAVSPGPLATRAASGLNCFEQFLTDAARRSPLRRLANIDDVGDMCSFLVSDAASVITGN 243 Query: 244 ITYVDGGFSRV 254 YVD G+ V Sbjct: 244 TLYVDAGYHIV 254 >gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 369 Score = 79.3 bits (194), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 81/260 (31%), Positives = 123/260 (47%), Gaps = 31/260 (11%) Query: 4 LQGKKILITGLLSNRSIAYGIAQACKREGAELAFTYVGERFKDRIT-KFAEEFDSK-LIF 61 L GK LITG + I +A A REGA LA Y+ E R T K + D+ L+ Sbjct: 123 LAGKVALITG--GDSGIGRAVALAYAREGARLAIVYLDEEEDARQTQKDVQALDAPCLLL 180 Query: 62 DCDVGSDEQINALFADLGKSWTQLDGLVH-AIGFAPSEAIAGDFLEGFSREGFKIAHDIS 120 DVGS E + + ++ +LD LV+ A P+++I E S+ + + Sbjct: 181 PGDVGSPEFCRSAVRAVMDAYGRLDVLVNNAAQQYPAQSI-----EEISQAQLEHTFRTN 235 Query: 121 AYSFPAMAKAAMPLLSSNAALLTLT----YLGSERVVPNYNTMGLAKASLEASVRYLAES 176 +S M K AMP L+ A ++ T Y GS+ +V +Y K ++ A R L++ Sbjct: 236 IFSMFYMVKEAMPFLTRGARIINTTSVTAYRGSKHLV-DY---AATKGAIVAFTRSLSQQ 291 Query: 177 LGPKGVRVNGVSAGPIKT------LAASGIKGFGKILGAVAATAPLRRNVTIEDVGNASA 230 L G+ VN V+ GPI T +A + FGK PL R ++V A Sbjct: 292 LASAGIHVNAVAPGPIWTPLIPASFSAEEVASFGK-------NVPLGRPGQPDEVAPAYV 344 Query: 231 FLLSDLASGITGEITYVDGG 250 FL ++ AS +TG++ + +GG Sbjct: 345 FLATEGASYMTGQVLHPNGG 364 >gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 60.8 bits (146), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 70/262 (26%), Positives = 113/262 (43%), Gaps = 28/262 (10%) Query: 4 LQGKKILITGLLSNRSIAYGIAQACKREGAELAFTYVGERFKDRITKFAEEFDSKLIFDC 63 L GK L+TG S I AQ +GA++ T + D + + D Sbjct: 5 LNGKIALVTGGTSG--IGLATAQELAAQGAQVFITGRRQAELDAAVASIGAAATGIRADA 62 Query: 64 DVGSDEQINALFADLGKSWTQLDGLVHAIGFAPSEAIAGDFLE--GFSREGFKIAHDISA 121 V S +++ ++A + KS +LD L G GD + + E F + Sbjct: 63 SVLS--ELDTVYAQIAKSAGRLDILFANAG-------GGDMMPLGAITEEHFDRIFGTNV 113 Query: 122 YSFPAMAKAAMPLLSSNAALLTLTYLGSERVVPNYNTMGLAKASLEASVRYLAESLGPKG 181 + A+PLL A+++ + S N++ +KA++ R A L + Sbjct: 114 RGVLFTVQKALPLLVDGASVILTSSTTSVLGTANFSVYSASKAAVRNFARSWALDLKDRA 173 Query: 182 VRVNGVSAGPIKTLAASGIKGFGKIL---------GAVAATAPLRRNVTIEDVGNASAFL 232 +RVN VS GPI+T G G+++ +A+ PL R +++G A AFL Sbjct: 174 IRVNVVSPGPIRT------PGLGELVPDDARQGLFDYLASQVPLGRLGEPQEIGKAVAFL 227 Query: 233 LSDLASGITGEITYVDGGFSRV 254 SD AS + G +VDGG ++V Sbjct: 228 ASDAASFVNGIELFVDGGMAQV 249 >gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 241 Score = 55.8 bits (133), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 70/254 (27%), Positives = 111/254 (43%), Gaps = 19/254 (7%) Query: 1 MAFLQGKKILITGLLSNRSIAYGIAQACKREGAELAFTYVGERFKDRITKFAEEFDSKLI 60 M+F GK ++ITG + R I A + GA++ R + + EE +++I Sbjct: 1 MSF-AGKTVIITG--AGRGIGRACALLLAQRGAQVIAL---ARTASDLARLQEEIGARVI 54 Query: 61 FDCDVGSDEQINALFADLGKSWTQLDGLVHAIGFAPSEAIAGDFLEGFSREGFK--IAHD 118 D+ A+ G + D L+++ G E++ LE S EG++ + + Sbjct: 55 A-VDLSDPAAARRAMAEAGTA----DFLINSAGINVLESV----LE-MSDEGYEAVLGVN 104 Query: 119 ISAYSFPAMAKAAMPLLSSNA-ALLTLTYLGSERVVPNYNTMGLAKASLEASVRYLAESL 177 + A A A M + A++ +T + R + +KA LE + R LA L Sbjct: 105 LRAALITCQAFARMRIAQGGGGAMVNITSIAGHRGFEQHLCYAASKAGLEGATRVLAREL 164 Query: 178 GPKGVRVNGVSAGPIKTLAASGIKGFGKILGAVAATAPLRRNVTIEDVGNASAFLLSDLA 237 GP G+RVN V+ T A G + A P R + EDV + A LLS A Sbjct: 165 GPHGIRVNAVAPTITLTELAEAAWSDPAKSGPMMARHPSARFASPEDVARSIAMLLSPDA 224 Query: 238 SGITGEITYVDGGF 251 + + G + VDGGF Sbjct: 225 AMLNGAVVPVDGGF 238 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 749,637 Number of extensions: 30288 Number of successful extensions: 170 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 172 Number of HSP's successfully gapped: 17 Length of query: 260 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 177 Effective length of database: 1,226,865 Effective search space: 217155105 Effective search space used: 217155105 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)