BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0067.p (261 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312738|ref|YP_003776830.1| short chain dehydrogenase [Herb... 448 e-127 gi|300310578|ref|YP_003774670.1| beta-hydroxybutyrate dehydrogen... 127 6e-31 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 124 8e-30 gi|300313779|ref|YP_003777871.1| short-chain dehydrogenase [Herb... 120 8e-29 gi|300310595|ref|YP_003774687.1| 3-oxoacyl-(acyl-carrier protein... 117 7e-28 >gi|300312738|ref|YP_003776830.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 261 Score = 448 bits (1153), Expect = e-127, Method: Compositional matrix adjust. Identities = 213/260 (81%), Positives = 240/260 (92%) Query: 1 MELNLQDQKVIVTAGAQGIGLAITEPFIKAGAQVHVCDINEAFLADARARLAGAAVSFSR 60 M+LNL+DQKVIVTAGAQGIGLAIT F++AGA VH+CD++E FLA ARAR A VS+SR Sbjct: 1 MDLNLKDQKVIVTAGAQGIGLAITAAFVEAGAHVHICDVSEDFLASARARFGHAPVSYSR 60 Query: 61 TDVARPEEVDAMFADLASRWDGRLDVLVNNAGIAGPTLPVEDIAPADWDQTIAINLTGPF 120 TDV+ EVDAMFADLA RW GRLDVL+NNAGIAGPT PVE++A +DWDQT+A+NLTGPF Sbjct: 61 TDVSSEREVDAMFADLAQRWSGRLDVLINNAGIAGPTSPVEEVALSDWDQTLAVNLTGPF 120 Query: 121 LCTRRAVPMLKQNGGGAIINISSVAGRLGFALRTPYSASKYGVIGMTETWAIELGPSNIR 180 LCTRRAVP+LK+NGGGAI+NISSVAGRLGFALRTPYSASKYGVIG+TETWAIELGPS+IR Sbjct: 121 LCTRRAVPLLKKNGGGAIVNISSVAGRLGFALRTPYSASKYGVIGLTETWAIELGPSHIR 180 Query: 181 VNAVLPGIVEGPRQERIVAAKAAAYGIEHEEMRARLLSKVSLRRMVSAQDIANQILFLCS 240 VNAVLPGIVEG RQERIVAAKAAAYGI HEEMR RLLS+VSLR+MV+A+DIANQ++FLCS Sbjct: 181 VNAVLPGIVEGARQERIVAAKAAAYGIGHEEMRQRLLSRVSLRKMVTAEDIANQVIFLCS 240 Query: 241 PAGAAISGQSLSVCGNVETL 260 PAGA+ISGQSLSVCGNVE L Sbjct: 241 PAGASISGQSLSVCGNVEVL 260 >gi|300310578|ref|YP_003774670.1| beta-hydroxybutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 127 bits (320), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 84/255 (32%), Positives = 143/255 (56%), Gaps = 8/255 (3%) Query: 5 LQDQKVIVTAGAQGIGLAITEPFIKAGAQVHVCDIN-EAFLADAR--ARLAGAAVSFSRT 61 L+D+ ++T A GIG I + + GA+V + D+ EA A A + G A++ + Sbjct: 3 LKDKVALITGSASGIGKEIAVEYARQGAKVVIADLALEAAKATAEEIVKEGGTAMAVA-M 61 Query: 62 DVARPEEVDAMFADLASRWDGRLDVLVNNAGIAGPTLPVEDIAPADWDQTIAINLTGPFL 121 +V +VD AD + + G LD++++NAGI + PV +++ +W + +AI+L G FL Sbjct: 62 NVTDETQVDKGVADAVNTYGG-LDIMISNAGIQIIS-PVAELSLENWRKMLAIHLDGAFL 119 Query: 122 CTRRAVP-MLKQNGGGAIINISSVAGRLGFALRTPYSASKYGVIGMTETWAIELGPSNIR 180 TR A+ M+KQ GG+II + SV L L+ PY +K+G++G+ +T A E IR Sbjct: 120 TTRAAMKAMIKQGRGGSIIYMGSVHSHLASPLKAPYVTAKHGLLGLAKTVAKEGAKDKIR 179 Query: 181 VNAVLPGIVEGPRQERIVAAKAAAYGIEHEEMRARLLSKVSLR-RMVSAQDIANQILFLC 239 N + PG V P E+ + +A +GI E++ +++ K ++ + QD+A +FL Sbjct: 180 TNVICPGFVRTPLVEKQIPEQAKEFGISEEDVIKKIMLKDTVDGEFTTTQDVAQTAVFLA 239 Query: 240 SPAGAAISGQSLSVC 254 + A++GQS+ V Sbjct: 240 AFPTNALTGQSVVVS 254 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 124 bits (311), Expect = 8e-30, Method: Compositional matrix adjust. Identities = 88/251 (35%), Positives = 127/251 (50%), Gaps = 22/251 (8%) Query: 11 IVTAGAQGIGLAITEPFIKAGAQVHVCDINEAFLADARARLAGAAVSFS------RTDVA 64 +VT GA GIG +I++ G V V DIN DA ++ A A V+ + D+ Sbjct: 12 LVTGGAMGIGASISQQLAAKGHTVLVADIN----LDAASKTADALVAAGHRAAPLQMDLG 67 Query: 65 RPEEVDAMFADLASRWDGRLDVLVNNAGIAGPTLPVEDIAPADWDQTIAINLTGPFLCTR 124 PE + A FA + + GR DVLVNNAG+A T D +W QT+ +N+TG L + Sbjct: 68 SPESIAAAFAHIEKDY-GRCDVLVNNAGVA-RTYSFLDYPLENWLQTMNVNVTGVLLAGQ 125 Query: 125 RAVPMLKQNGGGAIINISSVAGRLGFALRTPYSASKYGVIGMTETWAIELGPSNIRVNAV 184 A ++ + G I+NISS++G A RT Y SK VIG+T AIEL I VN+V Sbjct: 126 HAARLMVKQQWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNSV 185 Query: 185 LPGIVEGPRQERIVAAKAAAYGIEHEEMRARLLSKVSLRRMVSAQDIANQILFLCSPAGA 244 PG V+ P + + E+ R V +RR + +IA+ + +L + A Sbjct: 186 APGPVDTPMTQ----------AMHSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEESA 235 Query: 245 AISGQSLSVCG 255 I+G + V G Sbjct: 236 YITGHVIPVDG 246 >gi|300313779|ref|YP_003777871.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 120 bits (302), Expect = 8e-29, Method: Compositional matrix adjust. Identities = 81/254 (31%), Positives = 129/254 (50%), Gaps = 17/254 (6%) Query: 4 NLQDQKVIVTAGAQGIGLAITEPFIKAGAQVHVCDI----NEAFLADARARLAGAAVSFS 59 +L ++V++T G GIG A+ E F+ GAQV DI ++A +A + A A F Sbjct: 16 SLAGKRVVITGGGSGIGAALVEAFVGQGAQVCFLDIAAEPSQALVASLKD--APIAPRFF 73 Query: 60 RTDVARPEEVDAMFADLASRWDGRLDVLVNNAGIAGPTLPVEDIAPADWDQTIAINLTGP 119 ++ E + A F ++ + G +D+L+NNA D+ PA WD+ +A+NL Sbjct: 74 PCNLMNLEALRATFTEIETVMGG-VDILINNAA-NDDRHKTADVTPAYWDERLAVNLRHQ 131 Query: 120 FLCTRRAVPMLKQNGGGAIINISSVAGRLGFALRTPYSASKYGVIGMTETWAIELGPSNI 179 F C + +P +++ G I+N S++ LG T Y +K G+ GMT A + G + Sbjct: 132 FFCAQAVLPGMRERKQGVILNFGSISWHLGLPDLTLYMTAKAGIEGMTHGMARDFGRDGV 191 Query: 180 RVNAVLPGIVEGPRQERIVAAKAAAYGIEHEEMRARLLSKVSLRRMVSAQDIANQILFLC 239 RVNA++PG + PRQ + + EE A++L+ L V D+A LFL Sbjct: 192 RVNAIIPGAIRTPRQTLL-------WHTPEEE--AKILAAQCLPTRVDPHDVAALALFLS 242 Query: 240 SPAGAAISGQSLSV 253 S +GA +G+ V Sbjct: 243 SDSGAKCTGREYYV 256 >gi|300310595|ref|YP_003774687.1| 3-oxoacyl-(acyl-carrier protein) reductase [Herbaspirillum seropedicae SmR1] Length = 250 Score = 117 bits (294), Expect = 7e-28, Method: Compositional matrix adjust. Identities = 77/255 (30%), Positives = 130/255 (50%), Gaps = 13/255 (5%) Query: 2 ELNLQDQKVIVTAGAQGIGLAITEPFIKAGAQVHVCDINEAFLADARARLAG-AAVSFSR 60 + Q + I+T GAQG G A+ E ++ A+V + D++E LA+AR +L V + Sbjct: 3 HYDFQGRSAIITGGAQGFGYAVAERLLQGKAKVVLWDMDEKALAEARRKLESLGNVETVK 62 Query: 61 TDVARPEEVDAMFADLASRWDGRLDVLVNNAGIAGPTLPVEDIAPADWDQTIAINLTGPF 120 D++ EV + + +D+LV++AGIAG PV + +P +W + I I+L G F Sbjct: 63 VDISSQAEVQQAI-EATEKLTQSIDILVHSAGIAGQNNPVAEYSPEEWRRVIDIDLNGAF 121 Query: 121 LCTRRAVPMLKQNGGGAIINISSVAGRLGFALRTPYSASKYGVIGMTETWAIELGPSNIR 180 + V + G I+NI+S+AG+ G + YSA+K G+I +T++ E NI Sbjct: 122 YVNQVVVQRMIAQNYGRIVNIASIAGKEGNPTASAYSAAKAGMIALTKSLGKETATKNIA 181 Query: 181 VNAVLPGIVEGPRQERIVAAKAAAYGIEHEEMRARLLSKVSLRRMVSAQDIANQILFLCS 240 VNA+ P Q RI+ A+ I++ +LSK+ R V +++A + +L S Sbjct: 182 VNAITP----AAAQTRILEQCTQAH-IDY------MLSKIPRARFVKVEELAAMVAWLVS 230 Query: 241 PAGAAISGQSLSVCG 255 + + + G Sbjct: 231 EENSFTTASVFDLSG 245 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 754,952 Number of extensions: 29700 Number of successful extensions: 255 Number of sequences better than 1.0e-08: 48 Number of HSP's gapped: 164 Number of HSP's successfully gapped: 48 Length of query: 261 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 178 Effective length of database: 1,226,865 Effective search space: 218381970 Effective search space used: 218381970 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)