BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0061.p (286 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312742|ref|YP_003776834.1| outer membrane efflux protein [... 417 e-118 gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipo... 140 9e-35 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 140 1e-34 gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 135 4e-33 gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipo... 135 5e-33 >gi|300312742|ref|YP_003776834.1| outer membrane efflux protein [Herbaspirillum seropedicae SmR1] Length = 492 Score = 417 bits (1072), Expect = e-118, Method: Compositional matrix adjust. Identities = 230/255 (90%), Positives = 240/255 (94%) Query: 28 KXILANQRQLIPDLQQQEQDAQTALAILLGRPPQNFKVEEKGLAKIQMPRISAGLPSELL 87 + LANQRQLIPDLQQQEQDAQTALAILLGRPPQNFKVEEKGLA+IQMPRISAGLPSELL Sbjct: 237 RSALANQRQLIPDLQQQEQDAQTALAILLGRPPQNFKVEEKGLARIQMPRISAGLPSELL 296 Query: 88 TRRPDIRRAEANLAAANANVAVARASLFPSISLTGSTGAQSNALLSLFNGPNLFANLGTS 147 TRRPDIRRAEA+LAAANANVAVARA+LFPSI+LTGSTGAQS+ALLSLF+GPNLFANLG Sbjct: 297 TRRPDIRRAEASLAAANANVAVARAALFPSITLTGSTGAQSSALLSLFDGPNLFANLGAG 356 Query: 148 LIAPIFDGGNLRNQQALAEAQKEELVQVYRQRVITSLSEVEKALGAIRSLEERYRLKVNE 207 LIAPIFDGG LR Q+ LA AQKEELVQVYRQRVITSLSEVEKALGAIRSLEERYRLKV E Sbjct: 357 LIAPIFDGGKLRGQRDLAIAQKEELVQVYRQRVITSLSEVEKALGAIRSLEERYRLKVGE 416 Query: 208 VDQARFAFELAEIRYRAGAEDLLVVLDTQRTLSEAQNQLGQIKLQRLQATVSLYKALGGG 267 VDQARFAFELAEIRYRAGAEDLLVVLDTQRTLSEAQNQLGQIKLQRLQATVSLYKALGGG Sbjct: 417 VDQARFAFELAEIRYRAGAEDLLVVLDTQRTLSEAQNQLGQIKLQRLQATVSLYKALGGG 476 Query: 268 WQDKQVPAEPGAAST 282 WQD Q P + G +S Sbjct: 477 WQDSQAPGDSGTSSV 491 >gi|300313898|ref|YP_003777990.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 486 Score = 140 bits (354), Expect = 9e-35, Method: Compositional matrix adjust. Identities = 94/240 (39%), Positives = 128/240 (53%), Gaps = 1/240 (0%) Query: 41 LQQQEQDAQTALAILLGRPPQNFKVEEKGLAKIQMPRISAGLPSELLTRRPDIRRAEANL 100 L +Q + A+A L G P F + L I P I G+PS++L RRPDI AE + Sbjct: 243 LGRQRAQYEHAIATLTGTPAPAFSITPS-LTPITPPAIPVGVPSDVLERRPDIAAAERAM 301 Query: 101 AAANANVAVARASLFPSISLTGSTGAQSNALLSLFNGPNLFANLGTSLIAPIFDGGNLRN 160 AAANA + VA A+ +PS L S G S +LFN P+L +LG S +FDGG LR Sbjct: 302 AAANAQIGVASAAYYPSFMLQPSYGVDSRNWATLFNAPSLLWSLGVSASQSLFDGGRLRA 361 Query: 161 QQALAEAQKEELVQVYRQRVITSLSEVEKALGAIRSLEERYRLKVNEVDQARFAFELAEI 220 ++A E V YR+ V+T++ EVE + + +LE Y + AR E+A Sbjct: 362 GVDFSQAGYEATVASYRRTVLTAMQEVEDGITGLAALERAYAQSQLAIASARRVLEIASS 421 Query: 221 RYRAGAEDLLVVLDTQRTLSEAQNQLGQIKLQRLQATVSLYKALGGGWQDKQVPAEPGAA 280 RY GA L V+ Q++L ++ Q Q+ QRL +V L KALGG WQ + PAE A Sbjct: 422 RYEGGATPYLDVITAQQSLLNSERQAAQLMGQRLLVSVFLIKALGGDWQSRTPPAEAAGA 481 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 140 bits (353), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 95/238 (39%), Positives = 131/238 (55%), Gaps = 10/238 (4%) Query: 41 LQQQEQDAQTALAILLGR-----PPQNFKVEEKGLAKIQMPRISAGLPSELLTRRPDIRR 95 L++Q A+ AL +L+G+ PP +EK + I AGLPS+LLT RPDIR Sbjct: 245 LERQRAQAENALTLLVGKKIGELPPAQLLSDEKIVTDI-----PAGLPSDLLTNRPDIRS 299 Query: 96 AEANLAAANANVAVARASLFPSISLTGSTGAQSNALLSLFNGPNLFANLGTSLIAPIFDG 155 AE +L AANAN+ ARA+ FP ISLTG+ G+ S+ L LF + + G SL PIFD Sbjct: 300 AEQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSLSLPIFDF 359 Query: 156 GNLRNQQALAEAQKEELVQVYRQRVITSLSEVEKALGAIRSLEERYRLKVNEVDQARFAF 215 G LAE +K + V Y + + T+ EV AL A SL+E+ + + + + Sbjct: 360 GRNSANLDLAEVRKNKAVVTYEKAIQTAFREVADALVARGSLDEQVKAQEAFLAAQQERL 419 Query: 216 ELAEIRYRAGAEDLLVVLDTQRTLSEAQNQLGQIKLQRLQATVSLYKALGGGWQDKQV 273 +L ++R++ G L LD QR L AQ L Q + RL + LYKALGGG + V Sbjct: 420 KLTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGLNENTV 477 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 135 bits (340), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 91/217 (41%), Positives = 119/217 (54%), Gaps = 2/217 (0%) Query: 51 ALAILLGRPPQ-NFKVEEKGLAKIQMPRISAGLPSELLTRRPDIRRAEANLAAANANVAV 109 AL +L+G P GL + P + GLP+ LLTRRPD+ AE L AANAN+ Sbjct: 259 ALTLLVGSPVVLPVGQARAGLDAVFAP-LQPGLPALLLTRRPDVAAAEHRLMAANANIGA 317 Query: 110 ARASLFPSISLTGSTGAQSNALLSLFNGPNLFANLGTSLIAPIFDGGNLRNQQALAEAQK 169 ARA+ FPSISLTG+ G S L LF + S+ PIF G LRN AL+E ++ Sbjct: 318 ARAAFFPSISLTGNVGTASAELSGLFRDGSHAWTFSPSITLPIFTAGRLRNNLALSEVRR 377 Query: 170 EELVQVYRQRVITSLSEVEKALGAIRSLEERYRLKVNEVDQARFAFELAEIRYRAGAEDL 229 + V Y + V T+ EV AL A + L ++ + + V R L+ +RY GA Sbjct: 378 DLAVAAYEKTVQTAFREVADALAARQWLTQQLEIAQSAVRVQRERARLSALRYDNGAAPF 437 Query: 230 LVVLDTQRTLSEAQNQLGQIKLQRLQATVSLYKALGG 266 L VLD QR L AQ QL QI+ L ++V+LY ALGG Sbjct: 438 LDVLDAQRDLLSAQQQLVQIRRALLSSSVALYAALGG 474 >gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 497 Score = 135 bits (339), Expect = 5e-33, Method: Compositional matrix adjust. Identities = 77/204 (37%), Positives = 111/204 (54%) Query: 75 MPRISAGLPSELLTRRPDIRRAEANLAAANANVAVARASLFPSISLTGSTGAQSNALLSL 134 +P AGLPS++L RRPD+ AE L +ANA++ ARA+ FPSISLT + G S L L Sbjct: 291 LPATPAGLPSQVLLRRPDVLAAEHTLKSANADIGAARAAFFPSISLTANGGTASRTLGGL 350 Query: 135 FNGPNLFANLGTSLIAPIFDGGNLRNQQALAEAQKEELVQVYRQRVITSLSEVEKALGAI 194 F + S+ PIF+ G+LR A+ ++ V Y + + T+ EV AL Sbjct: 351 FKAGSGSWTFSPSINLPIFNAGSLRASLDSAKITRDIDVANYEKAIQTAFREVADALAVR 410 Query: 195 RSLEERYRLKVNEVDQARFAFELAEIRYRAGAEDLLVVLDTQRTLSEAQNQLGQIKLQRL 254 +L++R + + AF L++ RYR G + L L QRTL AQ L ++L Sbjct: 411 ATLDQRLDAQGKATAASEKAFRLSDARYRNGVDSYLTTLVAQRTLYSAQQTLISLRLTEQ 470 Query: 255 QATVSLYKALGGGWQDKQVPAEPG 278 ++LYK LGGGW ++ P +PG Sbjct: 471 SNRITLYKVLGGGWSEQTQPMQPG 494 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.130 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 778,478 Number of extensions: 27624 Number of successful extensions: 160 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 152 Number of HSP's successfully gapped: 19 Length of query: 286 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 202 Effective length of database: 1,222,130 Effective search space: 246870260 Effective search space used: 246870260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)