BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0047.p (447 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312188|ref|YP_003776280.1| hemolysin secretion-related tra... 583 e-168 gi|300312349|ref|YP_003776441.1| hemolysin-type secretion transm... 147 2e-36 gi|300311130|ref|YP_003775222.1| Type I antifreeze protein:HlyD ... 109 3e-25 >gi|300312188|ref|YP_003776280.1| hemolysin secretion-related transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 447 Score = 583 bits (1504), Expect = e-168, Method: Compositional matrix adjust. Identities = 294/447 (65%), Positives = 364/447 (81%) Query: 1 MKWFHWLAAWRDLWRRYLAVFFHFWKSRERLRSDYYNQQEADFLPAALALQESPSSPTLH 60 M H AW DLWRRYL VF H W+ R RSD++N+ EA+FLPAAL+LQE+P S +L Sbjct: 1 MSLRHRWQAWTDLWRRYLRVFGHAWRQRGHYRSDFFNKDEAEFLPAALSLQEAPDSRSLR 60 Query: 61 WTGRVLMCLVGFFFLWAIFGRIDIIVQASGKVIPSSRTKTIASVDVASVKALHVVEGQSV 120 WT R+L+ +V F LW++ GRIDI+V A+GKVIPS+RTKTIASVDV++V+A+ V EGQ V Sbjct: 61 WTARLLVGMVIFALLWSLLGRIDIVVSANGKVIPSARTKTIASVDVSAVRAILVSEGQLV 120 Query: 121 KAGELLIELDSSSSDAEHDKAADIAAQARLQLARTNAMRDAVASGLGPSLPQVQASSAAQ 180 KAGE+LIELDSSSSDAE DKAAD AQARLQ AR AM +A+ + P L QV + AQ Sbjct: 121 KAGEVLIELDSSSSDAERDKAADAVAQARLQAARAQAMMEAIQNLRTPELAQVPGVAQAQ 180 Query: 181 WQAAQRQLESQYRDFKSKLDRLNDEIARYAAALPLAEQLAHDYHSLLADNTVSRHAWQEK 240 W+A + QL +QY+DF+++L RL+DEI+RY AAL LA Q A DY +L+AD+TVSRHAW EK Sbjct: 181 WEAVRSQLVAQYQDFRARLGRLDDEISRYGAALKLATQRASDYQTLVADHTVSRHAWLEK 240 Query: 241 EQARIELRGQLADARNQRAALIAQTLKEAHDEGIDASKRIEAALQDQRRAGEHSKLLKLV 300 EQAR++L+GQL DARNQRA L+AQT K+AHD I+A K IEA+ QDQRRA EHSKLLKLV Sbjct: 241 EQARVDLQGQLDDARNQRATLVAQTRKDAHDSRIEADKTIEASQQDQRRADEHSKLLKLV 300 Query: 301 APVSGTVQQLAVHTVGGVVPAAQPLMQIVPQEAAVEVEAFLENKDIGFVEVGQQAHVKIE 360 APV+GTVQQL VHT+GGVVPAAQPLMQIVPQ++ +EVEA LENKD+GFVE+GQ A VKI+ Sbjct: 301 APVTGTVQQLTVHTIGGVVPAAQPLMQIVPQDSQIEVEAMLENKDVGFVEIGQAAEVKID 360 Query: 361 AYDYTKYGTVPATVRHVSHDAIEDEKRGLIYASKIVLGRNTLTADGRTLLLSPGMSVTVE 420 A+DYTKYGT+PA V H+S DAI+DEK+GL+Y+++IVL +++L DGR + LS GM+ VE Sbjct: 361 AFDYTKYGTLPARVVHISRDAIQDEKKGLLYSARIVLDQSSLIVDGRKVQLSAGMAANVE 420 Query: 421 IRTGTRRVIEYVLSPLIRHQRESLNER 447 I+TG RRVIEY LSPLIRHQ+E+L+ER Sbjct: 421 IKTGNRRVIEYALSPLIRHQKEALHER 447 >gi|300312349|ref|YP_003776441.1| hemolysin-type secretion transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 386 Score = 147 bits (370), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 123/395 (31%), Positives = 201/395 (50%), Gaps = 29/395 (7%) Query: 60 HWTGRVLMCLVGFFFLWAIFGRIDIIVQASGKVIPSSRTKTIASVDVASVKALHVVEGQS 119 W R+ M V LWA +ID + +A G+VI SRT+++ + D VK + V EG++ Sbjct: 14 QWVLRLSMASVLVLLLWAAVSQIDQVTRAQGQVIAVSRTQSVQAPDGGPVKQILVKEGEA 73 Query: 120 VKAGELLIELDSSSSDAE-HDKAADIAAQARLQLARTNAMRDAVASGLGPSLPQVQASSA 178 VK G+LL+ L+ +D A +AA R+ LAR A P L + Sbjct: 74 VKQGQLLMVLEQDRVQTSLNDSRAKVAA-LRISLARLRAEVYGHPLNFEPDLLDYK---- 128 Query: 179 AQWQAAQRQLESQYRDFKSKLDRLNDEIARYAAALPLAEQLAHDYHSLLADNTVSRHAWQ 238 ++ A Q+ L Y K +D+ ++A L LA Q SL V R Sbjct: 129 -EYIANQKDL---YVKRKILIDQ---DLASLQDMLGLANQELEMNKSLERTGDVGRADVL 181 Query: 239 EKEQARIELRGQLADARNQ--RAALIAQTLKEAHDEGIDASKRIEAALQDQRRAGEHSKL 296 ++ ++R Q+ + RN+ + AL+ T + E ++ + L+D+ + EH+ Sbjct: 182 RLQRNVADIRAQINNKRNKYFQEALVDMTKVQ---EDLNTQRE---QLEDRTQVLEHT-- 233 Query: 297 LKLVAPVSGTVQQLAVHTVGGVVPAAQPLMQIVPQEAAVEVEAFLENKDIGFVEVGQQAH 356 +L AP G V+ + V T+GGVV + +M+I+P + + VEA + D +V+VGQ A+ Sbjct: 234 -ELKAPADGVVKNIKVTTIGGVVRSGDVVMEILPVDNLI-VEAKVSTADSAYVKVGQAAN 291 Query: 357 VKIEAYDYTKYGTVPATVRHVSHDAIEDEKR-GLIYASKIVLGRNTLTADGR---TLLLS 412 VK++AYDY+ YGT+ V +VS D + DE R G + ++ + GR + + Sbjct: 292 VKLDAYDYSIYGTLNGHVVYVSPDTLLDETRQGAMPYYRVHIALEEAVFKGRKADAIQVK 351 Query: 413 PGMSVTVEIRTGTRRVIEYVLSPLIRHQRESLNER 447 PGM+ TVEI+ TR V+ ++ P+ + +ES+ ER Sbjct: 352 PGMTATVEIKANTRTVLSFLTKPIAKTLQESMGER 386 >gi|300311130|ref|YP_003775222.1| Type I antifreeze protein:HlyD family secretion protein [Herbaspirillum seropedicae SmR1] Length = 457 Score = 109 bits (273), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 109/410 (26%), Positives = 194/410 (47%), Gaps = 51/410 (12%) Query: 76 WAIFGRIDIIVQASGKVIPSSRTKTIASVDVASVKALHVVEGQSVKAGELLIELDSSSSD 135 W++F ID++V+ SG+++ S + I ++ V A+ V EG +VK G+ LI + ++ Sbjct: 58 WSVFAPIDMVVRGSGRIVASEHNQVIQHLEGGIVSAILVREGAAVKKGQTLISISDVRAN 117 Query: 136 AEHDKAADIAAQARLQLARTNA---------MRDAVASGLGPSLPQVQASSAAQWQAAQR 186 A+ + R ++AR +A M D + G P++ QA+ AA+ + Sbjct: 118 ADLGEGRVKILGLRAKIARLSAEASGASSLQMPDDLERG-DPAVQSEQAAFAARSSKLMQ 176 Query: 187 QLESQYRDFKSKLDRLNDEIARY---AAALPLAEQLAHDYHSLLADNTVSRHAWQEKEQA 243 ++ + L + I+R A+ L LA++ H +L N S + Sbjct: 177 EMSVLREQSAQRQAELGEAISRQKSQASELELAQRQYKLIHDMLVRNAAS-------QLE 229 Query: 244 RIELRGQLADARNQRAALIAQ------TLKEAHDEGIDASKRIEAALQDQRRAGEHSKLL 297 I+ ++ DA+++ AA A + EA + +A+ R + + + A E ++L Sbjct: 230 DIQAASRVQDAQSKLAATNAMIPRLNAAIAEAQAKQGEAASRFRSDARTELTAAE-TELA 288 Query: 298 KL----------------VAPVSGTVQQLAVHTVGGVVPAAQPLMQIVPQEAAVEVEAFL 341 +L AP+ G V ++ ++T+GGVV P++++ P + + +EA + Sbjct: 289 RLQEEIKSRNDRVSRSEVKAPMDGVVNRVMINTIGGVVRPGDPIIEMTPSDDKLVIEARI 348 Query: 342 ENKDIGFVEVGQQAHVKIEAYDYTKYGTVPATVRHVSHDAIEDE--KRG-LIYASKIVL- 397 D + G +A VK+ AYDY YG + V +S D + DE RG Y KI + Sbjct: 349 SPTDRAELVTGARARVKVSAYDYGVYGAMDGVVTEISADTVPDEGSPRGERFYRLKIEVK 408 Query: 398 -GRNTLTADGRTLLLSPGMSVTVEIRTGTRRVIEYVLSPLIRHQRESLNE 446 G + L + L L PGM+ + +I G R V +Y++SPL R + +L E Sbjct: 409 RGHDELQ---QKLALMPGMTASADIIVGRRTVWQYLMSPLSRFGQGALRE 455 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.131 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,326,449 Number of extensions: 53018 Number of successful extensions: 241 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 239 Number of HSP's successfully gapped: 3 Length of query: 447 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 359 Effective length of database: 1,203,190 Effective search space: 431945210 Effective search space used: 431945210 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)