BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0036.p (380 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312761|ref|YP_003776853.1| branched-chain amino acid ABC t... 733 0.0 gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC t... 357 e-100 gi|300310948|ref|YP_003775040.1| branched-chain amino acid ABC t... 249 3e-67 gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC t... 189 3e-49 gi|300313247|ref|YP_003777339.1| branched-chain amino acid ABC t... 127 1e-30 >gi|300312761|ref|YP_003776853.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 380 Score = 733 bits (1891), Expect = 0.0, Method: Compositional matrix adjust. Identities = 365/380 (96%), Positives = 376/380 (98%) Query: 1 MKFKSAIIPLTAAIGLAFAGAAHAQEVVKIAHVGPLSGPNAHMGKDNENGARMAVEELNA 60 MK KSAIIPLTAAIGLAFAGAA++QEVVKIAHVGPLSGPNAHMGKDNENGARMAV+ELNA Sbjct: 1 MKLKSAIIPLTAAIGLAFAGAAYSQEVVKIAHVGPLSGPNAHMGKDNENGARMAVDELNA 60 Query: 61 KGFTIGGKKVKFELLGEDDASDPKQATAVATKLVDQKVAAVIGHLNSGTTIPASKIYSDA 120 KGFTIGGKKVKFEL+GEDDASDPKQATAVATKLVDQKVAAVIGHLNSGTTIPASKIYSDA Sbjct: 61 KGFTIGGKKVKFELVGEDDASDPKQATAVATKLVDQKVAAVIGHLNSGTTIPASKIYSDA 120 Query: 121 GIPQVSPSATNPKYTQQGFKTTFRVVANDAQLGAALGKYAVQKLGTKQIAVIDDRTAYGQ 180 GIPQVSPSATNPKYTQQGFKT FRVVANDAQLGAALGKYAVQKLGTKQIAVIDDRTAYGQ Sbjct: 121 GIPQVSPSATNPKYTQQGFKTAFRVVANDAQLGAALGKYAVQKLGTKQIAVIDDRTAYGQ 180 Query: 181 GVAEEFAKGAKAAGGTIVGTQFTNDKATDFNAILTSLKGKKPDVIFFGGMDAVGGPMLRQ 240 GVA+EFAKGAKAAGGTIVGTQ+TNDKATDFNAILTSLKGKKPDV+FFGGMDAVGGPMLRQ Sbjct: 181 GVADEFAKGAKAAGGTIVGTQYTNDKATDFNAILTSLKGKKPDVVFFGGMDAVGGPMLRQ 240 Query: 241 MKQLGIGAKFMGGDGICTGSLPGLAGDGLGEDQVICAEAGGVDASGKKGMDDFRAAYKKK 300 MKQLGI AKFMGGDGICTGSLPGLAGDGLG+DQVICAEAGGVD +GKKGMDDFRAAYKKK Sbjct: 241 MKQLGISAKFMGGDGICTGSLPGLAGDGLGDDQVICAEAGGVDEAGKKGMDDFRAAYKKK 300 Query: 301 FGIDVVYNAAYAYDATMTVADAMAKAGSADPKKYLPELAKISHKGVTGVIAFDAKGDVKD 360 FGIDVVYNAAYAYDATMTVADAMAKAGSADPKKYLPELAKISHKGVTGVIAFDAKGD+KD Sbjct: 301 FGIDVVYNAAYAYDATMTVADAMAKAGSADPKKYLPELAKISHKGVTGVIAFDAKGDIKD 360 Query: 361 GSLTLYTYKGGQRTVLAVTK 380 GSLTLYTYKGGQRT+LAVTK Sbjct: 361 GSLTLYTYKGGQRTLLAVTK 380 >gi|300309516|ref|YP_003773608.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 378 Score = 357 bits (917), Expect = e-100, Method: Compositional matrix adjust. Identities = 194/365 (53%), Positives = 244/365 (66%), Gaps = 4/365 (1%) Query: 7 IIPLTAAIGLAFAGAAHAQEVVKIAHVGPLSGPNAHMGKDNENGARMAVEELNAKGFTIG 66 II + A+ AFA A Q VVKI PL+GP A GKDN+ G MA+E LNA+ T+G Sbjct: 10 IIAASLALIPAFAMAQETQ-VVKIGFSSPLTGPQASAGKDNQGGLMMAIERLNAQPITVG 68 Query: 67 GKKVKFELLGEDDASDPKQATAVATKLVDQKVAAVIGHLNSGTTIPASKIYSDAGIPQVS 126 GKK+KF+++ EDD +DPK AVA KL DQ V A++G NSG TIPAS++Y+DAGI V+ Sbjct: 69 GKKIKFDVIAEDDQADPKSGVAVAQKLADQGVKAIVGPYNSGVTIPASRVYNDAGI-VVA 127 Query: 127 PSATNPKYTQQGFKTTFRVVANDAQLGAALGKYAVQKLGTKQIAVIDDRTAYGQGVAEEF 186 A+NPK TQQGF T FRV A+D+QLG + YA ++L K++AVIDDRTAYGQG+A+EF Sbjct: 128 TVASNPKITQQGFATLFRVAASDSQLGGKMALYAAKELKFKRVAVIDDRTAYGQGLAQEF 187 Query: 187 AKGAKAAGGTIVGTQFTNDKATDFNAILTSLKGKKPDVIFFGGMDAVGGPMLRQMKQLGI 246 K AKA G +V T FTNDKATDF AILTS+KGKKPD +F GG GGP+ RQMKQLG+ Sbjct: 188 IKVAKANGIDVVSTDFTNDKATDFTAILTSIKGKKPDAVFLGGYAPQGGPIKRQMKQLGV 247 Query: 247 GAKFMGGDGICTGSLPGLAGDGLGEDQVICAEAGGVDASGKKGMDDFRAAYKKKFGIDVV 306 MGGDGIC+ + L GD +GE V C + G + K+G F Y+KK+ Sbjct: 248 DVPLMGGDGICSPEMGRLGGDAIGE-SVYCTQGGTMLDKAKEG-KVFSDEYQKKYNRPAE 305 Query: 307 YNAAYAYDATMTVADAMAKAGSADPKKYLPELAKISHKGVTGVIAFDAKGDVKDGSLTLY 366 A YD M +A AM +A S DPK++ P LAKIS+KGV G FDA D+K +T+Y Sbjct: 306 TYAVSFYDGMMLIAQAMKQANSVDPKQFGPALAKISYKGVAGQYDFDANHDLKQSPVTVY 365 Query: 367 TYKGG 371 +K G Sbjct: 366 RFKDG 370 >gi|300310948|ref|YP_003775040.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 394 Score = 249 bits (635), Expect = 3e-67, Method: Compositional matrix adjust. Identities = 130/342 (38%), Positives = 195/342 (57%), Gaps = 3/342 (0%) Query: 28 VKIAHVGPLSGPNAHMGKDNENGARMAVEELNAKGFTIGGKKVKFELLGEDDASDPKQAT 87 + I GPLSGP+ +GK +NG ++A+ E N +G IGGK + L+ +DD +DP A Sbjct: 40 LAIGFAGPLSGPSGGVGKSMQNGVQLALAEANRRGLRIGGKSWQLRLIAQDDRADPATAE 99 Query: 88 AVATKLVDQKVAAVIGHLNSGTTIPASKIYSDAGIPQVSPSATNPKYTQQGFKTTFRVVA 147 VA LV Q+V AV+GH SGT++ A+ +Y AG+ QV+PSA + + T + FR + Sbjct: 100 FVARSLVQQQVIAVVGHWTSGTSLAAAPVYHRAGVIQVTPSAMSRRLTAMSYPRIFRTIP 159 Query: 148 NDAQLGAALGKYAVQKLGTKQIAVIDDRTAYGQGVAEEFAKGAKAAGGTIVGTQFTNDKA 207 N+ LG YAV KL T+ I IDDRT +GQG+AE+FA+ A G +VG +DK Sbjct: 160 NNESLGRLAAGYAVDKLETRTIVTIDDRTPFGQGLAEQFARTASERGAQVVGRYSVSDKT 219 Query: 208 TDFNAILTSLKGKKPDVIFFGGMDAVGGPMLRQMKQLGIGAKFMGGDGICTGSLPGLAGD 267 +DFNA L + +PD+IFFGG+D G + + +++L + A+ M G T LP L Sbjct: 220 SDFNAALLEARKLQPDLIFFGGLDWQAGVLAKSIRRLKLHARLMASPG--TLGLPFLMRA 277 Query: 268 GLGEDQVICAEAGGVDASGKKGMDDFRAAYKKKFGIDVVYNAAYAYDATMTVADAMAKAG 327 G + + E G G FR Y + F D+ A +AY+A + + +AG Sbjct: 278 GPDANGALVLEPGPPQ-DKMPGWKSFRQRYSENFDSDMDLYAVFAYEAAQAIIQGIRQAG 336 Query: 328 SADPKKYLPELAKISHKGVTGVIAFDAKGDVKDGSLTLYTYK 369 SADP++ + ++ +GV+G +AF+ +GD+ S T+Y K Sbjct: 337 SADPERIAEAMHRLRFEGVSGPVAFNEEGDLLQPSFTMYEVK 378 >gi|300310991|ref|YP_003775083.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 373 Score = 189 bits (480), Expect = 3e-49, Method: Compositional matrix adjust. Identities = 128/350 (36%), Positives = 188/350 (53%), Gaps = 15/350 (4%) Query: 23 HAQEVVKIAHVGPLSGPNAHMGKDNENGARMAVEELNAKGFTIGGKKVKFELLGEDDASD 82 HA E VKIA GP++G A G + GA A+E++NA G G KFE + DDA + Sbjct: 25 HA-ETVKIAIAGPMTGAVAQYGDMVKAGALTAIEQINAAG---GANGNKFEAVMMDDACE 80 Query: 83 PKQATAVATKLVDQKVAAVIGHLNSGTTIPASKIYSDAGIPQVSPSATNPKYTQ-QGFKT 141 PKQA AVA K+V Q + VIGH+ SG+TIPAS IY + G+ ++PSAT P+ T+ + K Sbjct: 81 PKQAVAVANKIVSQGIKYVIGHVCSGSTIPASDIYENEGVVMITPSATAPQLTEAKPHKF 140 Query: 142 TFRVVANDAQLGAALGKYAVQKLGTKQIAVIDDRTAYGQGVAEEFAKGAKAAGGTIVGTQ 201 FR + D Q G A +Y ++KL K++AV+ D+ +YGQGVA AA +V + Sbjct: 141 IFRTIGRDDQQGPAAARYVIEKLKPKKVAVLHDKQSYGQGVASSVKAALDAAKVPVVVFE 200 Query: 202 FTNDKATDFNAILTSLKGKKPDVIFFGGMDAVGGPMLRQMKQLGIGAKFMGGDGICTGSL 261 N +D++AI+T LK + D ++FGG G ++RQ ++ GI A FMG +G+ + Sbjct: 201 GINAGDSDYSAIVTKLKSQGVDFVYFGGYHPEMGLIMRQAREQGIKAVFMGPEGVGNKDI 260 Query: 262 PGLAGDGLGEDQVICAEAGGVDASGKKGMDDFRAAYKKKFGIDVVYNAAY---AYDATMT 318 +AG E ++ A + AA K+ N + AY Sbjct: 261 TAIAGPA-SEGMLVTLPADFAADPANAALVKAFAAAKRD------PNGPFQMPAYSGVKI 313 Query: 319 VADAMAKAGSADPKKYLPELAKISHKGVTGVIAFDAKGDVKDGSLTLYTY 368 +ADA+A A S DP+K + S K G + +D KGD+K ++T+ Sbjct: 314 IADAIAGAKSTDPEKVAAYIHANSFKTPIGNVEYDKKGDLKSFKFVVFTW 363 >gi|300313247|ref|YP_003777339.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 127 bits (320), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 99/355 (27%), Positives = 165/355 (46%), Gaps = 17/355 (4%) Query: 19 AGAAHAQEVVKIAHVGPLSGPNAHMGKDNENGARMAVEELNAKGFTIGGKK---VKFELL 75 AG A ++ + + G LS P + + GA++A++E NA + K+ ++FELL Sbjct: 22 AGWAQERKTIALGFTGRLSEP---IAQSALQGAQLAIDEANA----LAAKRRSPLRFELL 74 Query: 76 GEDDASDPKQATAVATKLVDQKVAAVIGHLNSGTTIPASKIYSDAGIPQVSPSATNPKYT 135 +DD + A +A V +KVA VIG +S + + IY A IPQ+ +AT+ ++T Sbjct: 75 PQDDHGNENFAVNIARYYVKEKVAGVIGPWSSDAALATAAIYESAQIPQIGFTATSSQWT 134 Query: 136 QQGFKTTFRVVANDAQLGAALGKYAVQKLGTKQIAVIDDRTAYGQGVAEEFAKGAKAAGG 195 G + FRVV +L A L + A + L ++I VI AY + +A+ + + Sbjct: 135 GMGNRMPFRVVGGTRELAATLAEVATRSLQGRRIFVIQSDAAYSRALADTLVENLGGSAQ 194 Query: 196 TIVGTQF-TNDKATDFNAILTSLKGKKPDVIFFGGMDAVGGPMLRQMKQLGIGAKFMGGD 254 I ++ ++TDF+A L + + + DVI F G+ + + A+ + Sbjct: 195 QIALARYQVGRRSTDFHAALKAAEAHQADVIVFVGLYPQARAFIDGANRTRTTARLLLTG 254 Query: 255 GICTGSLPGLAGDGLGEDQVICAEAGGVDASGKKGMDDFRAAYKKKFGIDVVYNAAYAYD 314 G SLP L V+ E K + Y+++FG + YAYD Sbjct: 255 GASNLSLPAPLELRL---HVLEYEIARPHCPQWKVVTQ---TYQRRFGAAPTTYSFYAYD 308 Query: 315 ATMTVADAMAKAGSADPKKYLPELAKISHKGVTGVIAFDAKGDVKDGSLTLYTYK 369 A + A+ +AGS + K L ++ H G+ G ++FDA G TL Y+ Sbjct: 309 AASVLTAAIVQAGSDNGDKIATALHRMRHAGLHGTLSFDATGANAHPRYTLLRYQ 363 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.133 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,087,746 Number of extensions: 46095 Number of successful extensions: 164 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 150 Number of HSP's successfully gapped: 15 Length of query: 380 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 293 Effective length of database: 1,207,925 Effective search space: 353922025 Effective search space used: 353922025 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 131 (55.1 bits)