BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0030.p (400 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312803|ref|YP_003776895.1| two component sensor histidine ... 624 e-180 gi|300312674|ref|YP_003776766.1| two-component system transcript... 154 1e-38 gi|300311807|ref|YP_003775899.1| light-regulated signal transduc... 121 8e-29 gi|300311808|ref|YP_003775900.1| two component sensor histidine ... 75 1e-14 gi|300309362|ref|YP_003773454.1| transmembrane sensory transduct... 72 7e-14 >gi|300312803|ref|YP_003776895.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 401 Score = 624 bits (1609), Expect = e-180, Method: Compositional matrix adjust. Identities = 316/380 (83%), Positives = 345/380 (90%) Query: 18 MSERPILRLSDFIRSRMEPILEAWEGFAKTIQPPALTMDSKALRNHASLMLAAIADDLDK 77 MS RPILRLSDFIRSRMEPILEAWE FAKTI PPALTMDSKALRNHASLMLAAIADDL+K Sbjct: 1 MSGRPILRLSDFIRSRMEPILEAWEAFAKTIHPPALTMDSKALRNHASLMLAAIADDLEK 60 Query: 78 AQTEAQEIAKSEGRAPNAPNDTAAETHAIARLLSGFSVDQLASEYRALRSSVLRLWAREW 137 QTE QEIAKSEGRAP +DTAAETHAIARLLSGFSV+QLASEYRALRSSVLRLWA+EW Sbjct: 61 TQTEEQEIAKSEGRAPAHADDTAAETHAIARLLSGFSVEQLASEYRALRSSVLRLWAKEW 120 Query: 138 KEGNETNVDDITRFNEAIDQALAESLARYSKLLDQSRNLFLAILGHDLRNPLGATIQGAQ 197 KEG ETNVDDITRFNEAIDQALAESLARY+KLLDQSRNLFLAILGHDLRNPLGATI GAQ Sbjct: 121 KEGYETNVDDITRFNEAIDQALAESLARYTKLLDQSRNLFLAILGHDLRNPLGATIHGAQ 180 Query: 198 IILLAEDIDDRHRKIAARIYNSGQRMGKLVADLLDYTRTHLGTGLPLHLTSVNIGEIAQS 257 I+LLAEDIDDRHRKIAARIYNSGQRM KLVADLLDYTRTHLG+GLPL ++S N+G+IAQ Sbjct: 181 IMLLAEDIDDRHRKIAARIYNSGQRMSKLVADLLDYTRTHLGSGLPLEISSANVGDIAQR 240 Query: 258 VVEEISTSHPDREVTLKTSGDLKGKWDRERVAQAISNLLGNAVQHGLAEEPIRVEITAAQ 317 V++EISTSHPD+ +TLKTSG+L G+WD+ER+AQAISNLLGNAVQHG E I+VEI+A++ Sbjct: 241 VIDEISTSHPDKVITLKTSGELNGEWDKERIAQAISNLLGNAVQHGATGEAIKVEISASR 300 Query: 318 DEVIVSINNKAPRIPEEKLQSIFNPLVRLAADADEPAKQSGSMGLGLYIVREVASAHGGS 377 EV V+INNK P IPEEKL +IF+PLVRL A+ E + Q GSMGLGLYIVREVASAHGGS Sbjct: 301 GEVHVAINNKGPLIPEEKLHTIFDPLVRLVANGGEGSGQGGSMGLGLYIVREVASAHGGS 360 Query: 378 VTVGSSADEGTTFTIRLPKD 397 VT SS EGTTFTIRLP+D Sbjct: 361 VTASSSTAEGTTFTIRLPRD 380 >gi|300312674|ref|YP_003776766.1| two-component system transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 372 Score = 154 bits (389), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 84/222 (37%), Positives = 130/222 (58%), Gaps = 7/222 (3%) Query: 176 LFLAILGHDLRNPLGATIQGAQIILLAEDIDDRHRKIAARIYNSGQRMGKLVADLLDYTR 235 +F+AILGHDLRNPL A + A ++ ++ +K + +I N G+RM KL+ ++LD R Sbjct: 156 MFVAILGHDLRNPLSAVMMAATLLQRTYK-EEVAQKASQQILNGGKRMAKLIENMLDLAR 214 Query: 236 THLGTGLPLHLTSVNIGEIAQSVVEEISTSHPDREVTLKTSGDLKGKWDRERVAQAISNL 295 L G+PL S +GEI + VV+E ++P + + GD G WD +R+AQ +NL Sbjct: 215 ARLAGGIPLARASACLGEITERVVQEYQATYPGSVIERRIEGDDNGYWDGDRLAQVAANL 274 Query: 296 LGNAVQHGLAEEPIRVEITA-AQDEVIVSINNKAPRIPEEKLQSIFNPLVRLAADADEPA 354 LGNA+QHG PI + I ++ EV++ I N+ IP + L IF+P + A Sbjct: 275 LGNALQHGEPGHPICIGIDGRSEHEVVMVIANRGV-IPADLLPVIFDPF----RGGEREA 329 Query: 355 KQSGSMGLGLYIVREVASAHGGSVTVGSSADEGTTFTIRLPK 396 + +GLGL+I +++ AHGGS+++ S T T+RLP+ Sbjct: 330 GRKEGLGLGLFIAQQIVKAHGGSISMSSDDQLQTMVTVRLPR 371 >gi|300311807|ref|YP_003775899.1| light-regulated signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 732 Score = 121 bits (304), Expect = 8e-29, Method: Compositional matrix adjust. Identities = 71/228 (31%), Positives = 124/228 (54%), Gaps = 5/228 (2%) Query: 168 KLLDQSRNLFLAILGHDLRNPLGATIQGAQIILLAEDIDDRHRKIAARIYNSGQRMGKLV 227 L+ ++R LA LGHDLR+PL A + A++I + E ++ RI +S RM +L+ Sbjct: 506 NLVKRARETLLATLGHDLRDPLQAIMMAARMIEVREH-SPSSSNLSKRISSSSSRMHRLI 564 Query: 228 ADLLDYTRTHLGTGLPLHLTSVNIGEIAQSVVEEISTSHPDREVTLKTSGDLKGKWDRER 287 + +LD +R G GL + V++ ++ +V E ++PD E+ L+ + D +R Sbjct: 565 SQVLDISRLQSGMGLDIQRRPVDVRKMVSEIVSEARMAYPDNEIILEAEDCGEIALDGDR 624 Query: 288 VAQAISNLLGNAVQHGLAEEPIRVEITAAQDEVIVSINNKAPRIPEEKLQSIFNPLVRLA 347 ++Q +SNL+ N HG +P + + +D + ++++N P IPE ++F P + Sbjct: 625 ISQVVSNLISNTRHHGEIGKPSTLIVFRREDVLTIAVSNHGPAIPEPVRSNLFKPYKK-- 682 Query: 348 ADADEPAKQSGSMGLGLYIVREVASAHGGSVTVGSSADEGTTFTIRLP 395 ++ + + +GLGLYIV E+ HGG + V S + TFT+ LP Sbjct: 683 -ESLGNQRNTRGLGLGLYIVSEIVHGHGGKIAV-SCDNNIITFTVTLP 728 >gi|300311808|ref|YP_003775900.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 289 Score = 74.7 bits (182), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 60/224 (26%), Positives = 101/224 (45%), Gaps = 8/224 (3%) Query: 177 FLAILGHDLRNPLGATIQGAQIILLAEDIDDRHRKIAARIYNSGQRMGKLVADLLDYTRT 236 FLA L H+LR PL A + Q +L DR K I + + ++V DLLD +R Sbjct: 53 FLATLSHELRTPLNAIVGWTQYLLNDHSDADRLVKGLQVIERNARLQAQMVEDLLDMSRM 112 Query: 237 HLGTGLPLHLTSVNIGEIAQSVVEEISTSHPDREVTLKTS----GDLKGKWDRERVAQAI 292 G L L +++ +A+ + + + R++ L + G + G D R+ Q + Sbjct: 113 LAGK-LRLETRPMDLASVAEEAIAAVRPAAEARQIELHPALGQGGVILG--DPARLQQVV 169 Query: 293 SNLLGNAVQHGLAEEPIRVEITAAQDEVIVSINNKAPRIPEEKLQSIFNPLVRLAADADE 352 NLL NA++ + + + + D V + +++ I E L S+F+ + Sbjct: 170 WNLLMNAIKFTPRQGRVSLSLRQHDDTVALEVSDNGRGIKGEFLGSVFDRFTQEDVSTGS 229 Query: 353 PAKQSGSMGLGLYIVREVASAHGGSVTVGSSA-DEGTTFTIRLP 395 + +GLGL I R + HGG + S+ G FT+ LP Sbjct: 230 TGSTASGLGLGLAIARHLVELHGGQIRAASAGPGMGAAFTVTLP 273 >gi|300309362|ref|YP_003773454.1| transmembrane sensory transduction histidine kinase for cobalt/zinc/ cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 467 Score = 72.0 bits (175), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 64/248 (25%), Positives = 117/248 (47%), Gaps = 13/248 (5%) Query: 148 ITRFNEAIDQALAESLARYSKLLDQSRNLFLAILGHDLRNPLGATIQGAQIILLAEDIDD 207 + FNE +D+ L R + L Q F A L HD+R PL + Q+ L D Sbjct: 228 VESFNEMLDR-----LHRSFQQLSQ----FSADLAHDVRTPLNNLMVQTQVALGKPRGID 278 Query: 208 RHRKIAARIYNSGQRMGKLVADLLDYTRTHLGTGLPLHLTSVNIGEIAQSVVEEISTSHP 267 ++ + + +R+ +++ +L R + L +++ + + + Sbjct: 279 EYQALLSSNIEEYERLSRMMESMLFLARAE-NAHVVLQWQELDVAQELTRIADYFEGIAD 337 Query: 268 DREVTLKTSGDLKGKWDRERVAQAISNLLGNAVQHGLAEEPIRVEITAAQDEVIVSINNK 327 + +V L G + + D + +A+ NL+ NA+++ A+ IR+++ AA D + +S++N Sbjct: 338 EAQVRLVVQGAGRLQADATLLRRAVGNLVANAIRYTPADGVIRLQVDAAGDGMEISVSNP 397 Query: 328 APRIPEEKLQSIFNPLVRLAADADEPAKQSGSMGLGLYIVREVASAHGGSVTVGSSADEG 387 IP E+L+ +F+ R AD + S GL IVR + HGG +V SS + Sbjct: 398 GAGIPAEQLERLFDRFYR--ADPSRSSAGGSSGL-GLAIVRSIMMLHGGQASVTSSPGQL 454 Query: 388 TTFTIRLP 395 T F++R P Sbjct: 455 TVFSLRFP 462 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.130 0.360 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,130,743 Number of extensions: 44099 Number of successful extensions: 298 Number of sequences better than 1.0e-08: 33 Number of HSP's gapped: 275 Number of HSP's successfully gapped: 33 Length of query: 400 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 313 Effective length of database: 1,207,925 Effective search space: 378080525 Effective search space used: 378080525 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 132 (55.5 bits)