BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0027.p (336 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313025|ref|YP_003777117.1| chemotaxis signal transduction ... 551 e-158 gi|300311277|ref|YP_003775369.1| chemotaxis signal transduction ... 405 e-114 gi|300311026|ref|YP_003775118.1| chemotaxis response regulator r... 60 2e-10 gi|300312322|ref|YP_003776414.1| two component hybrid sensor his... 59 5e-10 gi|300311339|ref|YP_003775431.1| CheY chemotaxis protein [Herbas... 59 8e-10 >gi|300313025|ref|YP_003777117.1| chemotaxis signal transduction protein [Herbaspirillum seropedicae SmR1] Length = 320 Score = 551 bits (1421), Expect = e-158, Method: Compositional matrix adjust. Identities = 267/319 (83%), Positives = 291/319 (91%) Query: 17 MDNFQKEIDERTNLTSTNKFELLLFRVGTPDGSTDRPGELYGINVFKIREIVPMMPITAA 76 MD+FQKEIDERTNLTSTNKFELLLFR+GTPDGSTD+PGELYGINVFKIREIVPMM ITAA Sbjct: 1 MDSFQKEIDERTNLTSTNKFELLLFRLGTPDGSTDKPGELYGINVFKIREIVPMMDITAA 60 Query: 77 AGMTSPMLGMVNIRGQVMSVVDLSAVTGCRASQGLNILLITEFARHTQAFAVESVEEIVR 136 AGM SPM+GMVNIRGQVMSVVDL AV GC+A G NILLITEFARHTQAFAVESVEEIVR Sbjct: 61 AGMRSPMMGMVNIRGQVMSVVDLPAVAGCKAGHGNNILLITEFARHTQAFAVESVEEIVR 120 Query: 137 LDWSQVLPADAHAPGGLVTSIARMDTERDGGRLVQVLDVEHILNLVSPMKESIEPNKGGV 196 LDWSQVLPAD+H+PG LVTSIAR+D+E DGGRLVQVLDVEHILNLVSP+KE ++P G Sbjct: 121 LDWSQVLPADSHSPGSLVTSIARLDSEVDGGRLVQVLDVEHILNLVSPVKEQMQPAPPGA 180 Query: 197 RPVTLKPGTVVLAADDSRVARALIEQCLKELNLPFIMATDGLQAWNKLQQLSAEAEAEGK 256 + LKPG VVLAADDSR+ARALIEQCLK+LNLPFIM TDGLQAW KLQQLS A EGK Sbjct: 181 AELKLKPGAVVLAADDSRMARALIEQCLKDLNLPFIMETDGLQAWKKLQQLSQAAAQEGK 240 Query: 257 SVMDKVAMVLTDLEMPEMDGFTLTRKIKSSDKYKRLPVVIHSSLTGAANEDHVRSVGADG 316 + DKVAMVLTDLEMPEMDGFTLTRKIK+SD+YK+LPVVIHSSLTG+ANEDHVRSVGA+G Sbjct: 241 PLTDKVAMVLTDLEMPEMDGFTLTRKIKASDQYKQLPVVIHSSLTGSANEDHVRSVGANG 300 Query: 317 YVAKFAPSDLAAMLAKVLP 335 YVAKF+P +LAAML +VLP Sbjct: 301 YVAKFSPPELAAMLRQVLP 319 >gi|300311277|ref|YP_003775369.1| chemotaxis signal transduction protein [Herbaspirillum seropedicae SmR1] Length = 324 Score = 405 bits (1040), Expect = e-114, Method: Compositional matrix adjust. Identities = 205/325 (63%), Positives = 249/325 (76%), Gaps = 15/325 (4%) Query: 17 MDNFQKEIDERTNLTSTNKFELLLFRVGT-PDGSTDRPGELYGINVFKIREIVPMMPITA 75 MDNFQKEIDERTNLT++NKFELLLFR+GT P G EL+GINVFKIREIVPM P+T Sbjct: 1 MDNFQKEIDERTNLTASNKFELLLFRLGTDPHGER---SELFGINVFKIREIVPMPPVTK 57 Query: 76 AAGMTSPMLGMVNIRGQVMSVVDLSAVTGCRASQGLNILLITEFARHTQAFAVESVEEIV 135 AAG SPMLGMVNIRGQ++SV+DL AV GC GLNILL+TE+AR TQAFAVESV+EIV Sbjct: 58 AAGTQSPMLGMVNIRGQIISVIDLPAVVGCVPKTGLNILLVTEYARSTQAFAVESVDEIV 117 Query: 136 RLDWSQVLPADAHAPGGLVTSIARMDTERDGGRLVQVLDVEHILNLVSPMK-----ESIE 190 RL+WSQVL A+ + G VTSIAR+D ++D RL QVLDVE IL+ + P ES+E Sbjct: 118 RLEWSQVLSAETSSGGAFVTSIARLDADKDTSRLAQVLDVEQILHEIMPTDRDLKMESLE 177 Query: 191 PNKG-GVRPVTLKPGTVVLAADDSRVARALIEQCLKELNLPFIMATDGLQAWNKLQQLSA 249 + G+R PGTV +AADDS+VAR+LIEQ L+ + +P+ M G +AW K+Q+L+ Sbjct: 178 KSASHGIR-----PGTVAIAADDSKVARSLIEQGLRAMEIPYEMHVTGKEAWEKIQKLAR 232 Query: 250 EAEAEGKSVMDKVAMVLTDLEMPEMDGFTLTRKIKSSDKYKRLPVVIHSSLTGAANEDHV 309 EA AEG+ + DK++ VLTDLEMPEMDGFTLTR IK D K +PV+IHSSL+G+ANEDHV Sbjct: 233 EARAEGRPISDKISFVLTDLEMPEMDGFTLTRNIKREDYLKSIPVIIHSSLSGSANEDHV 292 Query: 310 RSVGADGYVAKFAPSDLAAMLAKVL 334 RSVG D YVAKF ++LAA + VL Sbjct: 293 RSVGGDAYVAKFEANELAATIYGVL 317 >gi|300311026|ref|YP_003775118.1| chemotaxis response regulator receiver domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 134 Score = 60.5 bits (145), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 44/129 (34%), Positives = 62/129 (48%), Gaps = 13/129 (10%) Query: 208 LAADDSRVARALIEQCLKELN-LPFIMATDGLQAWNKLQQLSAEAEAEGKSVMDKVAMVL 266 L DD R +I LKEL L A DG AW L+ + ++ V+ Sbjct: 8 LVVDDFSTMRRIIIGLLKELEYLKVTDADDGTSAWKILESGALP-----------ISFVI 56 Query: 267 TDLEMPEMDGFTLTRKIKSSDKYKRLPVVIHSSLTGAANEDHVRSVGADGYVAK-FAPSD 325 TD MP MDG L +KI+ + + KRLPV++ S+ N GADGY+ K F + Sbjct: 57 TDWNMPGMDGLGLLKKIRETPRLKRLPVLMVSAEAKKENIVLAAETGADGYIVKPFNAAT 116 Query: 326 LAAMLAKVL 334 L ++K+L Sbjct: 117 LDDKISKIL 125 >gi|300312322|ref|YP_003776414.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 775 Score = 58.9 bits (141), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 44/157 (28%), Positives = 64/157 (40%), Gaps = 16/157 (10%) Query: 172 VLDVEHILNLVSPMKESIE---PNKGGVRPVTLKPGTVVLAADDSRVARALIEQCLKELN 228 +LDV +L V + S ++ G P T VL DDS R L + L Sbjct: 617 ILDVADMLRTVEKLTSSGRLQTVDQDGAAPTTTAARKKVLVVDDSLTVRELERKLLTNRG 676 Query: 229 LPFIMATDGLQAWNKLQQLSAEAEAEGKSVMDKVAMVLTDLEMPEMDGFTLTRKIKSSDK 288 +A DG+ WN ++ D +V+TD++MP MDG L I+ + Sbjct: 677 YQVTVAVDGMDGWNAVR-------------ADSFDLVITDIDMPRMDGIELVSLIRGAAN 723 Query: 289 YKRLPVVIHSSLTGAANEDHVRSVGADGYVAKFAPSD 325 + LPV+I S + GAD Y K + D Sbjct: 724 LQSLPVMIVSYKDREEDRQRGLEAGADHYFTKSSFHD 760 >gi|300311339|ref|YP_003775431.1| CheY chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 131 Score = 58.5 bits (140), Expect = 8e-10, Method: Compositional matrix adjust. Identities = 41/134 (30%), Positives = 61/134 (45%), Gaps = 15/134 (11%) Query: 203 PGTVVLAADDSRVARALIEQCLKELNLPFI-MATDGLQAWNKLQQLSAEAEAEGKSVMDK 261 P T L DD R ++ LKEL + A DG+QA KL+ D+ Sbjct: 4 PNTKFLVVDDFSTMRRIVRNLLKELGYTNVDEAEDGVQALQKLRS-------------DQ 50 Query: 262 VAMVLTDLEMPEMDGFTLTRKIKSSDKYKRLPVVIHSSLTGAANEDHVRSVGADGYVAK- 320 V++D MP MDG T+ ++I+ +LPV++ ++ N GA+GYV K Sbjct: 51 FDFVVSDWNMPNMDGLTMLQEIRKDPALSKLPVLMVTAEAKKENIVAAAQAGANGYVVKP 110 Query: 321 FAPSDLAAMLAKVL 334 F + L L K+ Sbjct: 111 FTAATLDEKLGKIF 124 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 994,152 Number of extensions: 39181 Number of successful extensions: 191 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 195 Number of HSP's successfully gapped: 8 Length of query: 336 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 250 Effective length of database: 1,212,660 Effective search space: 303165000 Effective search space used: 303165000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)