BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0026.p (536 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310055|ref|YP_003774147.1| methyl-accepting chemotaxis tra... 592 e-170 gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis tra... 392 e-110 gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis tra... 376 e-105 gi|300310695|ref|YP_003774787.1| methyl-accepting chemotaxis sen... 375 e-105 gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis pro... 364 e-101 >gi|300310055|ref|YP_003774147.1| methyl-accepting chemotaxis transducer transmembrane protein I [Herbaspirillum seropedicae SmR1] Length = 547 Score = 592 bits (1526), Expect = e-170, Method: Compositional matrix adjust. Identities = 328/516 (63%), Positives = 413/516 (80%) Query: 1 MKNLKIGQRLTLGLAAVIFLLVCVAGFGLRQISSLNDRIEFLTSVDEGKLKALAKTQFGI 60 MKNLKIG RL LG AAV+ LLV VA FGL+Q+S LNDRI FLTSVDEGKL+AL+K QF + Sbjct: 1 MKNLKIGHRLGLGFAAVLILLVAVAAFGLQQVSRLNDRIVFLTSVDEGKLEALSKVQFAV 60 Query: 61 TLRAIAARNLTLITDPALQKNDALLVAKTQKDINDGLAELKRLLAESAAPEAERQLIDQL 120 LRAIAARNLTL+ A QKND LV +Q+DI+ G+AEL RL+ A+ ER+++++L Sbjct: 61 GLRAIAARNLTLVQSAADQKNDLDLVGSSQQDIDAGMAELGRLMQHPASSPQEREMLEKL 120 Query: 121 ADLEKQYYPVAVKVVELAMALKNDEARVVLNKECMPILQSVITHVQKINDYLYQNSQENA 180 LE QY PVAV +V+LA + + + AR L K+CMPIL VI V++ N L +NS+ N Sbjct: 121 RQLEAQYLPVAVNIVKLATSQQTEAARASLVKDCMPILNRVIAQVEQFNKLLRKNSEANV 180 Query: 181 AAAERAYSQSKLMMVGISVLALVVGMLIAWKLTRSIAAPLASAVEIARKVRDGDLTSRIK 240 AE AY+ +K M+ S+ AL +G ++AW LTRSI+ PLA AV +A+ V+ GDL SRI+ Sbjct: 181 EEAEAAYTLAKWTMLAASLAALTLGGMVAWLLTRSISRPLAQAVSVAQSVQSGDLGSRIE 240 Query: 241 VDSRDEVGELVGALKDMNESLAAIVTDVREGTHTIGAASGEIQRGTVDLSARTEQQASSL 300 V S+DE+G+L+ ALK MN+SL IV +VR+GT TI AS EI++G +DLS RTEQQA SL Sbjct: 241 VTSKDELGQLMSALKGMNDSLVNIVGEVRQGTDTIAVASDEIKQGNMDLSNRTEQQAGSL 300 Query: 301 EETASAMEQLTATVKQNSENAVQANRLANDASNIAAEGGEAVAVVVDTMRAISGSSREIV 360 +ETASAMEQLTATVKQN++NA +ANRLA++AS +AA GG AV VVDTM +IS SS +IV Sbjct: 301 QETASAMEQLTATVKQNADNAREANRLADNASQVAARGGAAVGRVVDTMNSISASSHKIV 360 Query: 361 EIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAAEVRALAHRSASAAKEIEALIN 420 +IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVA+EVR LA RSA AAKEIEALIN Sbjct: 361 DIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSAVAAKEIEALIN 420 Query: 421 QSVTRIATGEQQVNHAGATMGRVVESIKNVSGIVQEISVASAEQTRGLEQINQAIAQIDE 480 +SV ++ TGE+QV+ AG+TM VVESIK+V+ I+ +IS ASAEQT GLEQIN+A+AQIDE Sbjct: 421 ESVAKVVTGEKQVSEAGSTMHEVVESIKSVTHIMHDISAASAEQTSGLEQINRAVAQIDE 480 Query: 481 ATQRNAALVEEASAASSTMKQEAASLTQLVSTFKLS 516 ATQ+NAALVE+A+AA+++M+Q++ +LT++VS FKLS Sbjct: 481 ATQQNAALVEQAAAAAASMQQQSVALTEVVSIFKLS 516 >gi|300313938|ref|YP_003778030.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 533 Score = 392 bits (1006), Expect = e-110, Method: Compositional matrix adjust. Identities = 249/531 (46%), Positives = 335/531 (63%), Gaps = 10/531 (1%) Query: 1 MKNLKIGQRLTLGLAAVIFLLVCVAGFGLRQISSLNDRIEFLTSVDEGKLKALAKTQFGI 60 MKNLKIG RL +G A++ L+ + G+ ++ S+ D + +T + AL K + + Sbjct: 1 MKNLKIGVRLAIGFIALLMLMAVMVALGVWRLGSIGDATDDMTRI------ALKKERDAV 54 Query: 61 TLRAIAARNLTLITDPALQKNDALLVAKTQKDINDGLAELKRLL--AESAAPEAE-RQLI 117 A N + T ++ +D A QK I+ A + +L E+A +AE ++L Sbjct: 55 QWHAAIKEN-GVRTFAVMKSDDDAFQAYFQKQIDAQSARISQLQKEVEAAITDAEEKRLF 113 Query: 118 DQLADLEKQYYPVAVKVVELAMALKNDEARVVLNKECMPILQSVITHVQKINDYLYQNSQ 177 D++ L Y + ++ A + AR + + + ++ V + D+ Q Sbjct: 114 DEVGKLRAAYRATRQSIFDVKTAGDDARAREMTDTTLVKMMDDYADGVLRYADFQKQVID 173 Query: 178 ENAAAAERAYSQSKLMMVGISVLALVVGMLIAWKLTRSIAAPLASAVEIARKVRDGDLTS 237 + AAA R Y + M++ + + +V+G +AW LTRSI PL AV IA V GDLTS Sbjct: 174 QTAAAINRDYRSGRSMLLALGAIEIVLGAALAWLLTRSITRPLNQAVTIAETVAAGDLTS 233 Query: 238 RIKVDSRDEVGELVGALKDMNESLAAIVTDVREGTHTIGAASGEIQRGTVDLSARTEQQA 297 RI SRDE G+L+ ALK MN +L IV VR GT TI AS EI G +DLSARTE QA Sbjct: 234 RIDSSSRDETGKLLAALKTMNGNLLDIVGRVRMGTETINTASREIATGNLDLSARTEAQA 293 Query: 298 SSLEETASAMEQLTATVKQNSENAVQANRLANDASNIAAEGGEAVAVVVDTMRAISGSSR 357 SLEETASAMEQLT+TVKQN++NA QAN LA AS +A +GG V VV TM AI SS+ Sbjct: 294 GSLEETASAMEQLTSTVKQNADNARQANELAASASQVATQGGAVVDQVVATMGAIHASSQ 353 Query: 358 EIVEIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAAEVRALAHRSASAAKEIEA 417 +I +II VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVA+EVR+LA RSASAAKEI+ Sbjct: 354 KIADIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIKQ 413 Query: 418 LINQSVTRIATGEQQVNHAGATMGRVVESIKNVSGIVQEISVASAEQTRGLEQINQAIAQ 477 LI+ SV ++ G + V AG TM VV+S+ V+ +V+EI++AS EQ+ G+EQ+N AI Sbjct: 414 LIDDSVEKVDDGSRLVARAGDTMQEVVQSVARVTAVVREITLASQEQSDGIEQVNLAITH 473 Query: 478 IDEATQRNAALVEEASAASSTMKQEAASLTQLVSTFKLSSTAGAVVAEIPR 528 IDE TQ+NAALVE+A+AA+ +M+ +AASLT VS F+L A + + R Sbjct: 474 IDETTQQNAALVEQAAAAARSMQDQAASLTDAVSVFRLEDDAQVLAVAVQR 524 >gi|300312336|ref|YP_003776428.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 536 Score = 376 bits (966), Expect = e-105, Method: Compositional matrix adjust. Identities = 249/536 (46%), Positives = 349/536 (65%), Gaps = 13/536 (2%) Query: 3 NLKIGQRLTLGLAAVIFLLVCVAGFGLRQISSLND--RIEFLTSVDEGKLKALAKTQFGI 60 N+ IG+RLTLG A ++ + +AG GL ++ + R + +L A T Sbjct: 6 NMNIGRRLTLGFALILTFSIIIAGIGLWRLDHVAQATREMMAEPLKTERLMADWYTNLVA 65 Query: 61 TLRAIAARNLTLITDPALQKNDALLVAKTQKDINDGLAELKRLLAESAAPEAERQLIDQL 120 +R A + +DPAL A VA + K + +++ LL+ PE E+ L ++ Sbjct: 66 GIRRTIA--IAKSSDPALGPYFAAEVAASSKSSGEYQKQVEALLS---TPE-EKALFQKI 119 Query: 121 ADLEKQYYPVAVKVVELAMALKNDEARVVLNKECMPILQSVITHVQKINDYLYQNSQENA 180 +L K Y ++ + A +EA +L++ +P + ++K+ D+ + + A Sbjct: 120 GELRKLYLSSRDEINKAKAAGNTEEAMRILDQVFVPNATAYQDTMRKLVDFQRKEMDDTA 179 Query: 181 AAAERAYSQSKLMMVGISVLALVVGMLIAWKLTRSIAAPLASAVEIARKVRDGDLTSRIK 240 + ++S+ +++ + L L++G++ A LT I PL AV+++R V +GDL+S I Sbjct: 180 REIDAIAAKSRTLILVLEGLILLLGIVFARYLTLGITKPLHRAVDVSRCVAEGDLSSEIA 239 Query: 241 VDSRDEVGELVGALKDMNESLAAIVTDVREGTHTIGAASGEIQRGTVDLSARTEQQASSL 300 V SRDE G+L+ +LKDMN+SL IV++VR GT TI AS EI G +DLS+RTEQQASSL Sbjct: 240 VTSRDETGQLLQSLKDMNQSLRGIVSNVRSGTDTISTASSEIAAGNLDLSSRTEQQASSL 299 Query: 301 EETASAMEQLTATVKQNSENAVQANRLANDASNIAAEGGEAVAVVVDTMRAISGSSREIV 360 ETASAME+L +TV+QN++NA QA++LAN AS +A +GG V+ VVDTM AI+ SSR+IV Sbjct: 300 AETASAMEELISTVRQNADNARQASQLANSASAVAEQGGGVVSQVVDTMGAINASSRKIV 359 Query: 361 EIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAAEVRALAHRSASAAKEIEALIN 420 +IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVA+EVR LA RSASAAKEI+ LIN Sbjct: 360 DIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRTLAQRSASAAKEIKELIN 419 Query: 421 QSVTRIATGEQQVNHAGATMGRVVESIKNVSGIVQEISVASAEQTRGLEQINQAIAQIDE 480 SV ++ G + V AG TM VV S++ V+ IV EIS AS EQT G+EQIN AI Q+D+ Sbjct: 420 DSVGKVDDGSRLVEQAGNTMQEVVSSVRRVTDIVAEISAASTEQTHGIEQINLAITQMDQ 479 Query: 481 ATQRNAALVEEASAASSTMKQEAASLTQLVSTFKLSSTAGAVVAEIPRRQFETLPA 536 TQ+NAALVEEA+AA+++M+ +A L Q+VS F+LS V RR+ + P+ Sbjct: 480 VTQQNAALVEEAAAAAASMQTQAGRLMQMVSVFRLSEREPLV-----RREIDVTPS 530 >gi|300310695|ref|YP_003774787.1| methyl-accepting chemotaxis sensory transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 585 Score = 375 bits (964), Expect = e-105, Method: Compositional matrix adjust. Identities = 248/552 (44%), Positives = 348/552 (63%), Gaps = 51/552 (9%) Query: 1 MKNLKIGQRLTLGL---AAVIFLLVCVAGFGLRQISSLNDRIEFLTSVDEGKLKALAKT- 56 + +LKIG RL +G AA++ +LV VA ++ + N+ + T +L+A+A++ Sbjct: 3 LADLKIGTRLYMGFGLVAAILVILVSVAYTNFARLGAANE-LNVHTYEVRAQLRAMAESL 61 Query: 57 ------QFGITLRAIAAR----NLTLIT-DPALQKNDALLVAKTQKDINDGLAELKRLLA 105 Q G +L I A +L + T AL+K L T Sbjct: 62 INIETGQRGFSLSGIDASLEPYHLGMTTFKTALEKTRTLTSDNT---------------- 105 Query: 106 ESAAPEAERQLIDQLADLEKQYYPVAV-KVVELAMALKNDEARVVL---------NKECM 155 ++Q + +L+D E+++ A+ +EL A++ ++ K M Sbjct: 106 ------LQQQRLQELSDSEQRWLKSAIDPAIELRRAVREGREQMAAVVAFEQAGKGKSQM 159 Query: 156 PILQSVITHVQKINDYLYQNSQENAAAAERAYSQSKLMMVGISVLALVVGMLIAWKLTRS 215 ++ ++ + + L E AAA S + ++G +AL++ +L+AW L + Sbjct: 160 DSMRVLLDDLDRTESALLGERAEEAAALRSVTSTT---LIGGGAIALMLAVLLAWWLANN 216 Query: 216 IAAPLASAVEIARKVRDGDLTSRIKVDSRDEVGELVGALKDMNESLAAIVTDVREGTHTI 275 I PLA AV++AR+V GDLT+RI+V SRDE G L+GALKDMNESL IV+ VR GT I Sbjct: 217 ITRPLALAVQLARRVASGDLTARIEVRSRDETGALLGALKDMNESLVRIVSGVRRGTEMI 276 Query: 276 GAASGEIQRGTVDLSARTEQQASSLEETASAMEQLTATVKQNSENAVQANRLANDASNIA 335 AS EI G +DLS+RTEQQASSLEETAS+ME+LT+TV+QN+ NA +ANR+A AS+IA Sbjct: 277 ATASSEIATGNLDLSSRTEQQASSLEETASSMEELTSTVQQNTANAREANRMAQAASDIA 336 Query: 336 AEGGEAVAVVVDTMRAISGSSREIVEIISVIDGIAFQTNILALNAAVEAARAGEQGRGFA 395 + GG VA VVDTM I+ SSR+IV+IISVIDGIAFQTNILALNAAVEAARAGEQGRGFA Sbjct: 337 SSGGGVVARVVDTMEEINDSSRKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFA 396 Query: 396 VVAAEVRALAHRSASAAKEIEALINQSVTRIATGEQQVNHAGATMGRVVESIKNVSGIVQ 455 VVA+EVR+LA RSA+AAKEI+ALI+ SV ++ +G VN AG TM +VES++ V+ ++ Sbjct: 397 VVASEVRSLAQRSAAAAKEIKALISDSVAKVESGSLLVNEAGDTMREIVESVQRVTNMIN 456 Query: 456 EISVASAEQTRGLEQINQAIAQIDEATQRNAALVEEASAASSTMKQEAASLTQLVSTFKL 515 EIS A EQ G+EQ++QA+ Q+D+ TQ+NAALVE+A+AAS +++++A L Q VS F+L Sbjct: 457 EISAAGEEQRSGIEQVSQAVGQMDQVTQQNAALVEQAAAASQSLQEQAQELAQAVSVFRL 516 Query: 516 SSTAGAVVAEIP 527 + VA P Sbjct: 517 EAGQALEVAPEP 528 >gi|300312730|ref|YP_003776822.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 583 Score = 364 bits (934), Expect = e-101, Method: Compositional matrix adjust. Identities = 237/526 (45%), Positives = 338/526 (64%), Gaps = 14/526 (2%) Query: 1 MKNLKIGQRLTLGLAAVIFLLVCVAGFGLRQISSLNDRIEFLTSVDEGKLKALAKTQFG- 59 NL I RL + + +I ++ + GL +++ +N ++ + + D K A+ T G Sbjct: 3 FNNLSIATRLAIAFSILILVIAGIVALGLARLADINKALDLVVN-DRYKKIAIINTISGK 61 Query: 60 ITLRAIAARNLTLITDPALQKNDALLVAKTQKDINDGLAELKRLLAE----SAAPEAERQ 115 + AI+ RN L+TDPA +A QK I AE +L E A PEA+ + Sbjct: 62 VDDVAISLRNQLLMTDPAQ-------IAAEQKTIESLSAENTKLYDELTRLVANPEAKAK 114 Query: 116 LIDQLADLEKQYYPVAVKVVELAMALKNDEARVVLNKECMPILQSVITHVQKINDYLYQN 175 L D++ + K Y + ++ +L + D A ++ E +P+ + + + +++D + Sbjct: 115 L-DKVTETRKSYAALRKQIDKLQSSGDKDAAIKLMLSEMVPLQKIYFSQLDELSDTQQKL 173 Query: 176 SQENAAAAERAYSQSKLMMVGISVLALVVGMLIAWKLTRSIAAPLASAVEIARKVRDGDL 235 ++ AE AY ++++MV VLA IAW ++RSI PL+ AVE+A V GDL Sbjct: 174 MDQSVDQAEAAYETTRMIMVIAGVLAATFAAFIAWTISRSITRPLSRAVEVAETVAAGDL 233 Query: 236 TSRIKVDSRDEVGELVGALKDMNESLAAIVTDVREGTHTIGAASGEIQRGTVDLSARTEQ 295 T+ I S DE G L+ AL MN+ L AIV +VR GT ++ AS +I G +DLS+RTE+ Sbjct: 234 TAVIAAHSNDETGRLLQALAAMNQKLKAIVLEVRRGTDSMVTASTQIATGNLDLSSRTEE 293 Query: 296 QASSLEETASAMEQLTATVKQNSENAVQANRLANDASNIAAEGGEAVAVVVDTMRAISGS 355 QAS+LEETAS++E+LT++VKQN+++ +++ LA +AS +A +GG AV VV TM AI+ S Sbjct: 294 QASALEETASSLEELTSSVKQNADHTRKSSELAANASQVANQGGLAVKQVVQTMGAINDS 353 Query: 356 SREIVEIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAAEVRALAHRSASAAKEI 415 S +IV+IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVA+EVR LA RSA+AAKEI Sbjct: 354 SHKIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRGLAQRSATAAKEI 413 Query: 416 EALINQSVTRIATGEQQVNHAGATMGRVVESIKNVSGIVQEISVASAEQTRGLEQINQAI 475 + LIN SV ++ +G + V AG+TM VV SI+ VS IV EIS A+ EQ+ G+EQ+NQAI Sbjct: 414 KELINDSVNQVTSGTELVAQAGSTMDAVVNSIEQVSHIVGEISAATREQSDGIEQVNQAI 473 Query: 476 AQIDEATQRNAALVEEASAASSTMKQEAASLTQLVSTFKLSSTAGA 521 Q+D TQ+NAALVEEA+AA+ ++ +A L LV F+L G+ Sbjct: 474 MQMDNTTQQNAALVEEAAAAAQALQDQAQRLLDLVGVFRLDEVGGS 519 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.126 0.321 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,162,159 Number of extensions: 39747 Number of successful extensions: 525 Number of sequences better than 1.0e-08: 71 Number of HSP's gapped: 278 Number of HSP's successfully gapped: 73 Length of query: 536 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 446 Effective length of database: 1,193,720 Effective search space: 532399120 Effective search space used: 532399120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 133 (55.8 bits)