BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0015.0013.p (153 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311201|ref|YP_003775293.1| threonine efflux protein [Herba... 80 7e-17 gi|300314064|ref|YP_003778156.1| threonine efflux protein [Herba... 58 3e-10 >gi|300311201|ref|YP_003775293.1| threonine efflux protein [Herbaspirillum seropedicae SmR1] Length = 219 Score = 80.1 bits (196), Expect = 7e-17, Method: Compositional matrix adjust. Identities = 56/161 (34%), Positives = 85/161 (52%), Gaps = 11/161 (6%) Query: 1 VAAVGMGALLMASQMAFTVVKVLGAAYLIWLGCKLFFARV--ELGGQPLEPQQRSIAGYV 58 +A G+G+LL+AS+ AF V+KV GA YLI+LG + ARV E G+ L+ S + Sbjct: 56 IAGAGVGSLLVASETAFNVIKVAGALYLIYLGFSQWRARVPSEEQGEALDAANASARAHA 115 Query: 59 RKE--------FLVAIGNPKAILVFTAFFPQFVVPENYLS-SFLTLGAIFLALEVVAIAA 109 R+ FL NPK I+ A PQF+ + L L L +A+++V + Sbjct: 116 RQTWWQRCMTGFLTNATNPKGIVFMVAVLPQFIDHDRPLWLQLLVLAVTMVAVDLVVMHG 175 Query: 110 YAYAGTRLSGLMQNARGLRWMNRISGGMMIFFGTLLALAKR 150 YA+A +RL ++ + +R NR GG+++ G L KR Sbjct: 176 YAFAASRLQRFFRSQKAIRAQNRFFGGVLMLVGAGLFFFKR 216 >gi|300314064|ref|YP_003778156.1| threonine efflux protein [Herbaspirillum seropedicae SmR1] Length = 210 Score = 58.2 bits (139), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 38/84 (45%), Positives = 52/84 (61%), Gaps = 5/84 (5%) Query: 17 FTVVKVLGAAYLIWLGCKLFFARVELGGQPLEPQQRSIAGYVRKEFLVAIGNPKAILVFT 76 F ++ LGAAYL +LG +L FAR E GG + P S A R+ FLV + NPKAI+ + Sbjct: 79 FHGLQYLGAAYLAYLGARLLFARGE-GGGTVVP--FSNAAEFRRGFLVTLFNPKAIVFYM 135 Query: 77 AFFPQFVVPENYLS--SFLTLGAI 98 AFFP F+ P +F+T+GA+ Sbjct: 136 AFFPLFLDPATQQGALTFITMGAV 159 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.330 0.141 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 476,748 Number of extensions: 18374 Number of successful extensions: 86 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 84 Number of HSP's successfully gapped: 2 Length of query: 153 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 76 Effective length of database: 1,255,275 Effective search space: 95400900 Effective search space used: 95400900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 126 (53.1 bits)