BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0014.0028.p (150 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309980|ref|YP_003774072.1| two component transmembrane sen... 296 6e-82 gi|300313819|ref|YP_003777911.1| two component sensor histidine ... 100 5e-23 gi|300312689|ref|YP_003776781.1| transmembrane sensor kinase tra... 92 1e-20 gi|300312679|ref|YP_003776771.1| periplasmic sensor signal trans... 90 9e-20 gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine ... 80 6e-17 >gi|300309980|ref|YP_003774072.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 390 Score = 296 bits (757), Expect = 6e-82, Method: Compositional matrix adjust. Identities = 143/152 (94%), Positives = 146/152 (96%), Gaps = 2/152 (1%) Query: 1 MSFTQRIDLGFEQPDHPILITGNPVMLRELLSNLLDNALHYTPHDGNGRVTVRARTDEGG 60 MSFTQRIDLGFEQPDHPILITGNPVMLRELLSNLLDNALHYTPHDGNGRVTVRARTDEGG Sbjct: 239 MSFTQRIDLGFEQPDHPILITGNPVMLRELLSNLLDNALHYTPHDGNGRVTVRARTDEGG 298 Query: 61 GLAILEVEDNGPGIPVAERTNVFERFYRILGTPSTGS--GLGLAIVREIAQQHEAIVEIT 118 GLAILEVEDNGPGIPVAER NVFERFYRILGTPSTGS GLGLAIVREIAQQHEA +EIT Sbjct: 299 GLAILEVEDNGPGIPVAERANVFERFYRILGTPSTGSGLGLGLAIVREIAQQHEAALEIT 358 Query: 119 HNPRSSDPKYPGSLFRVSFRMMPRTPFFDDIT 150 NPRS+DPKYPG +FRVSFRMMPRTPFFDDIT Sbjct: 359 SNPRSTDPKYPGCVFRVSFRMMPRTPFFDDIT 390 >gi|300313819|ref|YP_003777911.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 485 Score = 100 bits (249), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 62/141 (43%), Positives = 86/141 (60%), Gaps = 10/141 (7%) Query: 2 SFTQRIDLGFEQPDHPILITGNPVMLRELLSNLLDNALHYTPHDGNGRVTVRARTDEGG- 60 + ++ I L FE+ + + I N ++L EL+ NLLDNA+ YTP G+ + VRA++ G Sbjct: 344 AVSREIGLAFEE-EGDVSIEANALLLHELMVNLLDNAIRYTPAGGHVTLRVRAQSSPGQP 402 Query: 61 GLAILEVEDNGPGIPVAERTNVFERFYRILGTPST---GSGLGLAIVREIAQQHEAIVEI 117 +A+LEVED+GPGIP AER VFE FYR T G+GLGL+IVREIA H A V + Sbjct: 403 TVALLEVEDSGPGIPAAEREKVFEPFYRAGATQHVNPGGTGLGLSIVREIAAMHRASVTL 462 Query: 118 THNPRSSDPKYPGSLFRVSFR 138 + S G + R+ F+ Sbjct: 463 DAPAQGS-----GLVVRIVFQ 478 >gi|300312689|ref|YP_003776781.1| transmembrane sensor kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 459 Score = 92.4 bits (228), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 50/117 (42%), Positives = 64/117 (54%), Gaps = 4/117 (3%) Query: 1 MSFTQRIDLGFEQPDHPILITGNPVMLRELLSNLLDNALHYTPHDGNGRVTVRARTDEGG 60 ++ + IDLG E ++ GN L +L NL+DNAL YTP G V + + + Sbjct: 327 LALKKGIDLGLETTLESAMVNGNEAQLMGMLMNLIDNALRYTPEGGKVTVGLEKKRSQ-- 384 Query: 61 GLAILEVEDNGPGIPVAERTNVFERFYRILGTPSTGSGLGLAIVREIAQQHEAIVEI 117 I+ V DNGPGIP R VFERFYR GSGLGLAIVREI Q + + + Sbjct: 385 --MIITVSDNGPGIPAEVRERVFERFYRNAAPDQPGSGLGLAIVREIVQASQGSIRL 439 >gi|300312679|ref|YP_003776771.1| periplasmic sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 89.7 bits (221), Expect = 9e-20, Method: Compositional matrix adjust. Identities = 55/137 (40%), Positives = 79/137 (57%), Gaps = 12/137 (8%) Query: 5 QRIDLGFEQPDHPILITGNPVMLRELLSNLLDNALHYTPHDGNGRVTVRARTDEGGGLAI 64 +RIDLGF+ P + G+ +L +L+ NL+DNA+ Y+P +G VTVR ++ + Sbjct: 332 KRIDLGFDL--RPARLRGDRFLLSDLIDNLIDNAVRYSP--PHGTVTVRCLEEQDA--TV 385 Query: 65 LEVEDNGPGIPVAERTNVFERFYRILGTPSTGSGLGLAIVREIAQQHEAIVEITHNPRSS 124 L VED+GPGI R +F+RFYR GSGLGLAIVREIA H ++ + + Sbjct: 386 LSVEDSGPGIAPEHRELIFDRFYRA-NDKVAGSGLGLAIVREIAHDHGGVISVDCKEQGQ 444 Query: 125 DPKYPGSLFRVSFRMMP 141 G++F V F + P Sbjct: 445 -----GTIFTVRFPLAP 456 >gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 444 Score = 80.5 bits (197), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 55/121 (45%), Positives = 69/121 (57%), Gaps = 13/121 (10%) Query: 7 IDLGF-EQPD--HPILITGNPVMLRELLSNLLDNALHYTPHDGNGRVTVRARTDEGGGLA 63 IDLG +Q D P+ +TGN LR L +NL+DNAL YTP G V V T G Sbjct: 309 IDLGLSDQGDDGRPLRVTGNADALRILFNNLVDNALRYTPAGGVVDVGVDLST---AGYC 365 Query: 64 ILEVEDNGPGIPVAERTNVFERFYRILGT-------PSTGSGLGLAIVREIAQQHEAIVE 116 + V+D+GPGI +E VF+RFYR + + GSGLGLAIVR+IA H VE Sbjct: 366 TVVVQDSGPGIAESELPRVFDRFYRAQHSQEGSGSRAAFGSGLGLAIVRQIADLHRVPVE 425 Query: 117 I 117 + Sbjct: 426 L 426 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.141 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 479,196 Number of extensions: 18142 Number of successful extensions: 127 Number of sequences better than 1.0e-08: 22 Number of HSP's gapped: 118 Number of HSP's successfully gapped: 22 Length of query: 150 Length of database: 1,619,870 Length adjustment: 77 Effective length of query: 73 Effective length of database: 1,255,275 Effective search space: 91635075 Effective search space used: 91635075 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)