BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0014.0025.p (395 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310449|ref|YP_003774541.1| 4-hydroxybenzoate transporter t... 222 4e-59 gi|300310481|ref|YP_003774573.1| major facilitator superfamily p... 177 2e-45 gi|300310645|ref|YP_003774737.1| major facilitator superfamily b... 173 2e-44 gi|300310615|ref|YP_003774707.1| major facilitator superfamily p... 88 1e-18 gi|300310666|ref|YP_003774758.1| major facilitator superfamily p... 74 3e-14 >gi|300310449|ref|YP_003774541.1| 4-hydroxybenzoate transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 447 Score = 222 bits (566), Expect = 4e-59, Method: Compositional matrix adjust. Identities = 140/381 (36%), Positives = 208/381 (54%), Gaps = 11/381 (2%) Query: 1 VLSAALFGLAAGALTSGPLADRYGRRKILLLSVLVMGVACMGSAFAGNLQELTILRFLTG 60 V A LFG+ G+L P+ DRYGRR +L+LS L + + + L +L +LRF+TG Sbjct: 69 VFGAGLFGMLLGSLVLTPVGDRYGRRPVLILSTLFFALCMLATPMVTTLDQLLVLRFITG 128 Query: 61 LGLGAAMPNAVTLTSEYSPAARRAMLTNAMFCGFPLGAALGGFLAAWMIPLWGWRSVLML 120 GLG+ MPNA+ L E+SP++ R + CGF +GAA GGF++A +IP +GW +V + Sbjct: 129 FGLGSIMPNAMALVGEFSPSSSRVTRMMLVSCGFTVGAAAGGFVSAALIPAYGWHAVFWV 188 Query: 121 GGAAPLLLLLVMLPNLPESVRHMVAKGYAAERIGAVLRRISSSAAAFNR--FTLAETGPQ 178 GGA PL+L L ML LPES++ +V ++ LR++ + ++ + ET + Sbjct: 189 GGAVPLVLGLAMLVWLPESIQFLVLHRRPRTQVARWLRKLDPNIVIDDKTEVVVKETKAE 248 Query: 179 GEAPTGKSGIALLFEPRYLVGTCMLWLAYFMGLVIFYALVNWMPVLFKDAGLAPKTATLI 238 G +A LF T +LWL FM L+ Y L NW+P L +DAG + A LI Sbjct: 249 GMP------VAALFRDGRAGVTVLLWLISFMNLINLYFLSNWLPTLIRDAGYSTSMAVLI 302 Query: 239 AALFPLGGV-GAILFGWLMDRYNANRVIAIGFAATAVAVFAIGQAAGNVGVLTVVVFVAG 297 +GGV G + G + R+ RV+ F +++ IG+ A +L V V VAG Sbjct: 303 GTSLQVGGVIGTLSLGRFIQRFGFTRVLGSCFLLACLSIALIGKLATVPALLFVAVIVAG 362 Query: 298 TLMNTAQSSLPALAAAYYPTRGRATGVAWMLGVGRFGGIAGSFLVAELTRRQLGFSEVFA 357 + Q ++ ALA +YPT R+TG+ W LG+GR G + G + +L Q + +F Sbjct: 363 FCIVGGQPAVNALAGTFYPTTLRSTGIGWALGIGRIGSVVGPVIGGQLIALQWTNASLFL 422 Query: 358 VVAVAALVS--TVALLVKQAS 376 AV AL+S T+A L + A Sbjct: 423 AAAVPALISALTIARLHRTAQ 443 >gi|300310481|ref|YP_003774573.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 460 Score = 177 bits (448), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 128/374 (34%), Positives = 199/374 (53%), Gaps = 8/374 (2%) Query: 2 LSAALFGLAAGALTSGPLADRYGRRKILLLSVLVMGVACMGSAFAGNLQELTILRFLTGL 61 ++A L G+ G+ +G ADR+GRR ++ SV V GVA AF+ ++ + ILRF GL Sbjct: 70 VAAGLVGMGIGSAFAGLFADRFGRRMAIIGSVFVFGVATSLIAFSPDVTTIAILRFCAGL 129 Query: 62 GLGAAMPNAVTLTSEYSPAARRAMLTNAMFCGFPLGAALGGFLAAWMIPLWGWRSVLMLG 121 G+G A+P++ T+T+E+SPA RR + A PLG L G A ++P +GWR + ++G Sbjct: 130 GIGGALPSSTTMTAEFSPARRRTLAVTATIVCVPLGGMLAGLFANAVLPHYGWRGLFLIG 189 Query: 122 GAAPLLLLLVMLPNLPESVRHMVAKGYAAERIGAVLRRISSSAAAFNRFTLAETGPQGEA 181 G P+ +++ +PES R++ + + + +L+R+S +A F A +A Sbjct: 190 GLLPVAFSVLLWFVIPESPRYLARHPHRWDELRRLLQRMSRPVSADTGFADARE----QA 245 Query: 182 PTGKSGIALLFEPRYLVGTCMLWLAYFMGLVIFYALVNWMPVLFKDAGLAPKTATLIAAL 241 ++G LF T LW+A+FM L+ Y+ +W+P + GL+P A Sbjct: 246 IETRAGFGALFASGQARDTMALWVAFFMNLLAVYSAFSWLPTMLAGEGLSPSVAGAGLTA 305 Query: 242 FPLGGV-GAILFGWLMDRYNAN-RVIAIGFAATAVAVFAIG-QAAGNVGVLTVVVFVAGT 298 + GGV GA+L + R+ + ++ AATA A+F A +L V + + G Sbjct: 306 YNFGGVFGALLCALFITRFGSRLPMLLCCLAATASALFLRSLDVASQTTLLVVGIGLHGL 365 Query: 299 LMNTAQSSLPALAAAYYPTRGRATGVAWMLGVGRFGGIAGSFLVAELTRRQLGFSEVFAV 358 +N QS++ AL A YPT+ RATG A L GR G I +F L Q G + A+ Sbjct: 366 FVNAVQSTMYALCAYIYPTQVRATGTAAALAFGRLGAILSAF-AGALVITQGGAAGYLAM 424 Query: 359 VAVAALVSTVALLV 372 +A A L+ VALLV Sbjct: 425 LAGAMLMVFVALLV 438 >gi|300310645|ref|YP_003774737.1| major facilitator superfamily benzoate permease [Herbaspirillum seropedicae SmR1] Length = 455 Score = 173 bits (438), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 137/370 (37%), Positives = 194/370 (52%), Gaps = 10/370 (2%) Query: 3 SAALFGLAAGALTSGPLADRYGRRKILLLSVLVMGVACMGSAFAGNLQELTILRFLTGLG 62 S ALFG+ GA GPLADR GR+K + +S ++ V+ S FA + E + RFL GLG Sbjct: 63 SYALFGMMFGAFIFGPLADRIGRKKGVAISFVLFSVSTFLSGFATSPTEFGVYRFLAGLG 122 Query: 63 LGAAMPNAVTLTSEYSPAARRAMLTNAMFCGFPLGAALGGFLAAWMIPLWGWRSVLMLGG 122 G MPNAV L +EY+P R+ L MF G+ LG L L WM+P +GW+S + Sbjct: 123 CGGLMPNAVALMNEYAPKRLRSTLVAIMFSGYSLGGVLCAGLGIWMLPNFGWQS-MFFAA 181 Query: 123 AAPLLLLLVMLPNLPESVRHMVAKGYAAERIGAVLRRISSSAAAFNRFTLAETGPQGE-A 181 A PL+LL V+L LPESV ++ +G E+ A+L ++ A L + +G A Sbjct: 182 AVPLVLLPVILWKLPESVGFLLRQG-RLEQARAMLTQVDPQARIATDTQLVQADVKGAGA 240 Query: 182 PTGKSGIALLFEPRYLVGTCMLWLAYFMGLVIFYALVNWMPVLFKDAGLAPKTATLIAAL 241 P + LF+ VGT M+W+++F L++ YAL +W+P L AG + ++ Sbjct: 241 P-----MLHLFKQDRAVGTLMIWVSFFCCLLMVYALGSWLPKLMASAGYSLGSSLSFLLA 295 Query: 242 FPL-GGVGAILFGWLMDRYNANRVIAIGFAATAVAVFAIGQAAGNVGVLTVVVFVAGTLM 300 G VGAI GWL DR+ +V+ F VA+ +G + VL +++ VAG Sbjct: 296 LNFGGVVGAIGGGWLGDRFTLPKVVVSFFTVGTVAIALLGFNS-PTPVLYLLIIVAGATT 354 Query: 301 NTAQSSLPALAAAYYPTRGRATGVAWMLGVGRFGGIAGSFLVAELTRRQLGFSEVFAVVA 360 Q L A A +YP R+TG+ W GVGR G I G L L L F V A Sbjct: 355 IGTQILLYANTAQFYPLAIRSTGLGWASGVGRSGAIVGPLLGGALMAAALPLKMNFLVFA 414 Query: 361 VAALVSTVAL 370 + LV+ +A+ Sbjct: 415 IPGLVAALAM 424 >gi|300310615|ref|YP_003774707.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 478 Score = 87.8 bits (216), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 93/339 (27%), Positives = 152/339 (44%), Gaps = 22/339 (6%) Query: 1 VLSAALFGLAAGALTSGPLADRYGRRKILLLSVLVMGVACMGSAFAGNLQELTILRFLTG 60 ++++ G GAL G +A+RYGR ++V +M V + A G+ L + RFL G Sbjct: 84 LIASGYLGQVIGALFFGWMAERYGRVPSASVTVGLMSVLSIVCALTGSFHMLFLCRFLQG 143 Query: 61 LGLGAAMPNAVTLTSEYSPAARRAMLTNAMFCGFPLGAALGGFLAAWMIPLWGWRSVLML 120 +G+G +P A T +E S A R FPLG + A+++P +GW + ++ Sbjct: 144 IGVGGEVPVAATYINELSQAHGRGKFFVLYELIFPLGLLAAAQVGAFLVPRFGWEYMFLV 203 Query: 121 GGAAPLLLLLVMLPNLPESVRHMVAKGY--AAERIGAVLRRISSSAAAFNRFTLAETGPQ 178 GG P +L+ +++ LPES R ++A+G AE++ +R + +S R A+T Q Sbjct: 204 GG-IPGILVALLITRLPESPRWLIARGRLDDAEKL---IRTLEASTP--KRNLDAQTDKQ 257 Query: 179 -----------GEAPTGKSGIALLFEPRYLVGTCMLWLAYFMGLVIFYALVNWMPVLFKD 227 G + LF P Y T ++WL + + + NW+P L+K Sbjct: 258 AIEQRINALAAGAGKRQRGSWKELFSPVYRGRTIIVWLLWASSYFVANGINNWLPSLYKT 317 Query: 228 AGLAPKTATL-IAALFPLGGVGAIL-FGWLMDRYNANR-VIAIGFAATAVAVFAIGQAAG 284 P +L +A++ + A+L L+DR R IA + A+ A Sbjct: 318 VYHLPLQESLRMASVSNVLSACAVLGCALLIDRVGRRRWAIASFILSGALLALLGVLKAE 377 Query: 285 NVGVLTVVVFVAGTLMNTAQSSLPALAAAYYPTRGRATG 323 + + + A +M T L YPTR RA G Sbjct: 378 SAWSVMFLASSAYAVMGTTTVLLYLYTPEIYPTRMRAIG 416 >gi|300310666|ref|YP_003774758.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 438 Score = 73.6 bits (179), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 93/382 (24%), Positives = 170/382 (44%), Gaps = 25/382 (6%) Query: 8 GLAAGALTSGPLADRYGRRKILLLSVLVMGVACMGSAFAGNLQELTILRFLTGLGLGAAM 67 G+ GA G LADR GRR +L+ +V++ + SAFA + L LR LTG+G+G + Sbjct: 70 GMLLGAFVFGRLADRIGRRPVLMGAVVIDACFGVASAFAPDFTWLLALRLLTGIGVGGTL 129 Query: 68 PNAVTLTSEYSPAARRAMLTNAMFCGFPLGAALGGFLAAWMIPLWG---WRSVLMLGGAA 124 P T+ +E+ P+ARR + + +G LA + WG WR + + G Sbjct: 130 PVDYTMMAEFLPSARRGRWLVMLESFWAIGTICLAVLALVAVS-WGNDAWRVIFFVTG-L 187 Query: 125 PLLLLLVMLPNLPESVRHMVAKGYAAERIGAVLRRISSSAAAFNRFTLAETGPQGEAPTG 184 P L+ +V+ +PES ++ G ++ VL R++ + P Sbjct: 188 PALVGVVLRFYVPESPMYLNRSG-KSDAARQVLERVAKVNGSST-----PIPPLQPEVVE 241 Query: 185 KSGIALLFEPRYLVGTCMLWLAYFMGLVIFYALVNWMPVLFKDAG---LAPKTATLIAAL 241 + ++ LF + L+LA+ + + +Y + ++PV G + + + AL Sbjct: 242 RKPMSALFSAALRRRSLSLYLAWGLISIAYYGVFVYLPVKLGSEGFGFMRGQIFLIFLAL 301 Query: 242 FPLGGVGAILFGWLMDRYNANRVIAIGF---AATAVAVFAIGQAAGNVGVLTVVVFVAGT 298 L G +G ++R+ + +GF +A ++++G+ + ++ + Sbjct: 302 VQLPGYALSAYG--VERWGRKPTL-VGFLVLSAVGCMLYSLGK---DPMLVVGSTLLLSF 355 Query: 299 LMNTAQSSLPALAAAYYPTRGRATGVAWMLGVGRFGGIAGSFLVAELTRRQLGFSEVFAV 358 + ++L A YPT RA+G+ V RFGG+ +VA + F+ A+ Sbjct: 356 SLLGTWAALYAFTPEVYPTDLRASGMGTAGAVARFGGLFAPAIVAPVMASH--FTLALAM 413 Query: 359 VAVAALVSTVALLVKQASDRNR 380 ++ + VA+L RNR Sbjct: 414 LSAFLVAGAVAILCVDVESRNR 435 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.139 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,042,607 Number of extensions: 39010 Number of successful extensions: 366 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 344 Number of HSP's successfully gapped: 19 Length of query: 395 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 308 Effective length of database: 1,207,925 Effective search space: 372040900 Effective search space used: 372040900 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 131 (55.1 bits)